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The map label for this gene is nemA [H]

Identifier: 116696100

GI number: 116696100

Start: 2450103

End: 2451212

Strand: Direct

Name: nemA [H]

Synonym: H16_B2164

Alternate gene names: 116696100

Gene position: 2450103-2451212 (Clockwise)

Preceding gene: 116696099

Following gene: 116696101

Centisome position: 84.12

GC content: 66.58

Gene sequence:

>1110_bases
ATGAGCCCCCTCTTCAAGCCCTTTGCCATGGCCGAGCAAACGCTATCCAACCGGATCGCCATGGCGCCGCTGACCCGTTC
GCGCAACCCGGACGGGGTGCCCAACGATCTCAACGCGCTCTACTACGGCCAGCGTGCCGACGCCGGCCTGATCGTGACCG
AAGGCACGGTGATCTCGCCCAGCGCGCAGGGATTCCTGTTCAATCCGGGCCTCTACACGCCGCAACAGGTTGAAGGCTGG
CGCAAGGTGACCGACGCGGTGCATGCGAAGCGCGGAACGATCTTCACGCAGCTCTGGCACGTCGGCCGGGTGAGCCACGT
CTCGATCCAGCCTGGCGGCATCCAGCCGGTCAGCGCAACCGACCAGGTTGCGAAGAACACCAAGGCCTGGGGCTATACCG
CCGACGGCACGCCCGGTGCGGTCGATGTATCCGCGCCGCGCGCGCTGGCCAGCGGTGAGGTCTACGGCGTAATCGCCGAT
TTCGCGGAAGCCGCGGCCAATGCGATCGCCGCCGGCTTCGACGGCGTGGAACTTCACGGTGCCAATGGCTACCTGATCGA
ACAGTTCCTGAACCCGACGGTGAACGACCGCACCGACGAATTCCGTGGCGACACGCTGGAAGGCCGTACCCGCTTTGTAC
TGGCTGCCATCGACGCCGTGGCAGCCCGGATCGGCGCGCATCGCACGGCCATCCGCCTGTCTCCCTATGGCGGACTGGGC
GACATGGCCGAGTACCCTGAGCTGGAGCAGACCTATCTCCATCTGGCCGACGAGCTCTCGAAGCGGAATATCGCCTACGT
CCACCTCATGGACCAGAGCACGCGCGGCAGCTCGGCCACGCCGGGCGACTTCCTGGCAAGGTTCCGACGTCGCTTCACGG
GTGCGCTGATCCTTGCGGGTGGCATGACACGCGCACGCGCCGAGCAGCTGATTGCCGATGACCTGATCGACATTGCCGCC
TTCGGCGAGCCGTTCATCGCCAACCCCGATCTGGTTGCCCGGCTTGAGAACAACTGGCCGCTGGCCTCGTCGGACCGCGC
GCACCACTACGGTGGCGGCGCCGAGGGCTACACGGACTATCCCGCCTACGACGGCGCCGCCGCAGCCTGA

Upstream 100 bases:

>100_bases
TTTGATGCCAGATCAAAAGTATTTGACCGATACACGGCTTAATCTGCAAAGATGAACCGGGCAAACTGCGCTCCATCGAA
ACCCATTCCCTGGAGCACAA

Downstream 100 bases:

>100_bases
ACTGCGCCAGACCACCTCGCGCAGCTTCACGCGGCGCTTCGGGCAGCCGCCCGGGGCGGGGCGGGTGTCCGCACACGGAT
AGCAAGGCTGCACTCAGTCC

Product: NADH:flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSPLFKPFAMAEQTLSNRIAMAPLTRSRNPDGVPNDLNALYYGQRADAGLIVTEGTVISPSAQGFLFNPGLYTPQQVEGW
RKVTDAVHAKRGTIFTQLWHVGRVSHVSIQPGGIQPVSATDQVAKNTKAWGYTADGTPGAVDVSAPRALASGEVYGVIAD
FAEAAANAIAAGFDGVELHGANGYLIEQFLNPTVNDRTDEFRGDTLEGRTRFVLAAIDAVAARIGAHRTAIRLSPYGGLG
DMAEYPELEQTYLHLADELSKRNIAYVHLMDQSTRGSSATPGDFLARFRRRFTGALILAGGMTRARAEQLIADDLIDIAA
FGEPFIANPDLVARLENNWPLASSDRAHHYGGGAEGYTDYPAYDGAAAA

Sequences:

>Translated_369_residues
MSPLFKPFAMAEQTLSNRIAMAPLTRSRNPDGVPNDLNALYYGQRADAGLIVTEGTVISPSAQGFLFNPGLYTPQQVEGW
RKVTDAVHAKRGTIFTQLWHVGRVSHVSIQPGGIQPVSATDQVAKNTKAWGYTADGTPGAVDVSAPRALASGEVYGVIAD
FAEAAANAIAAGFDGVELHGANGYLIEQFLNPTVNDRTDEFRGDTLEGRTRFVLAAIDAVAARIGAHRTAIRLSPYGGLG
DMAEYPELEQTYLHLADELSKRNIAYVHLMDQSTRGSSATPGDFLARFRRRFTGALILAGGMTRARAEQLIADDLIDIAA
FGEPFIANPDLVARLENNWPLASSDRAHHYGGGAEGYTDYPAYDGAAAA
>Mature_368_residues
SPLFKPFAMAEQTLSNRIAMAPLTRSRNPDGVPNDLNALYYGQRADAGLIVTEGTVISPSAQGFLFNPGLYTPQQVEGWR
KVTDAVHAKRGTIFTQLWHVGRVSHVSIQPGGIQPVSATDQVAKNTKAWGYTADGTPGAVDVSAPRALASGEVYGVIADF
AEAAANAIAAGFDGVELHGANGYLIEQFLNPTVNDRTDEFRGDTLEGRTRFVLAAIDAVAARIGAHRTAIRLSPYGGLGD
MAEYPELEQTYLHLADELSKRNIAYVHLMDQSTRGSSATPGDFLARFRRRFTGALILAGGMTRARAEQLIADDLIDIAAF
GEPFIANPDLVARLENNWPLASSDRAHHYGGGAEGYTDYPAYDGAAAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=45.8333333333333, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1789463, Length=349, Percent_Identity=29.512893982808, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=254, Percent_Identity=30.3149606299213, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=309, Percent_Identity=27.831715210356, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=255, Percent_Identity=31.7647058823529, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559804, Length=329, Percent_Identity=26.4437689969605, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=26.7806267806268, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540738, Length=355, Percent_Identity=24.2253521126761, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=36.7609254498715, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=35.4922279792746, Blast_Score=208, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39524; Mature: 39392

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLFKPFAMAEQTLSNRIAMAPLTRSRNPDGVPNDLNALYYGQRADAGLIVTEGTVISP
CCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHEEECCCCCCCEEEECCEEECC
SAQGFLFNPGLYTPQQVEGWRKVTDAVHAKRGTIFTQLWHVGRVSHVSIQPGGIQPVSAT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCHH
DQVAKNTKAWGYTADGTPGAVDVSAPRALASGEVYGVIADFAEAAANAIAAGFDGVELHG
HHHHHCCCCCCEECCCCCCEEECCCCCHHHCCCEEEHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLIEQFLNPTVNDRTDEFRGDTLEGRTRFVLAAIDAVAARIGAHRTAIRLSPYGGLG
CCCHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
DMAEYPELEQTYLHLADELSKRNIAYVHLMDQSTRGSSATPGDFLARFRRRFTGALILAG
CHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEEC
GMTRARAEQLIADDLIDIAAFGEPFIANPDLVARLENNWPLASSDRAHHYGGGAEGYTDY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCC
PAYDGAAAA
CCCCCCCCC
>Mature Secondary Structure 
SPLFKPFAMAEQTLSNRIAMAPLTRSRNPDGVPNDLNALYYGQRADAGLIVTEGTVISP
CCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHEEECCCCCCCEEEECCEEECC
SAQGFLFNPGLYTPQQVEGWRKVTDAVHAKRGTIFTQLWHVGRVSHVSIQPGGIQPVSAT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCHH
DQVAKNTKAWGYTADGTPGAVDVSAPRALASGEVYGVIADFAEAAANAIAAGFDGVELHG
HHHHHCCCCCCEECCCCCCEEECCCCCHHHCCCEEEHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLIEQFLNPTVNDRTDEFRGDTLEGRTRFVLAAIDAVAARIGAHRTAIRLSPYGGLG
CCCHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
DMAEYPELEQTYLHLADELSKRNIAYVHLMDQSTRGSSATPGDFLARFRRRFTGALILAG
CHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEEC
GMTRARAEQLIADDLIDIAAFGEPFIANPDLVARLENNWPLASSDRAHHYGGGAEGYTDY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCC
PAYDGAAAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]