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The map label for this gene is yafC [H]

Identifier: 116696099

GI number: 116696099

Start: 2449092

End: 2449994

Strand: Direct

Name: yafC [H]

Synonym: H16_B2163

Alternate gene names: 116696099

Gene position: 2449092-2449994 (Clockwise)

Preceding gene: 116696096

Following gene: 116696100

Centisome position: 84.09

GC content: 66.0

Gene sequence:

>903_bases
ATGAAGACGACCATCGACGAACTGCTGGCCTTTGCCGCCGTGGTGGACACCGGCTCGATCACGGCGGCCGCCGAGCAGCT
CAAGCTGACGGTCTCCGCCGCCAGCCGCACCCTGGGCCGGCTGGAGGAGAAACTGCAGACCACGCTGCTGCGGCGAACCA
CGCGGCGGCTGGAGCTGACCGAGGAAGGGGCGGCATTCCTGGAACACGCCCGCGCCATCCTGGCGATGGTTGACGAGGCG
GAAGAGCAGATCGCGGCGCGCCGCCTGCGGCCGGTTGGGCGCTTGCGGGTGGATGCGGCAACGCCGTTCATGCTGCATGT
GATCGTGCCGCTGCTGGAGGGCTTTCGCGCCAGCTACCCGGAAGTGGAACTGGAACTCAACTCGAACGAAGGCATCATCG
ACCTGATCGAAAAGCGCACCGATGTGGCGTTCCGCATCGGCCAGCTGAAGGACTCAACGCTGCATGCGCGCCCCATCGGC
ACCAGCCGAATCCGCATCCTGGCCAGTCCCGGCTACCTGAAGCGCCACGGCACGCCCACCAAGCCGGCGCAGCTCGCGCA
GCATGCGCTGCTGGGCTTCACGCAGCCGGAATCGCTGAACGACTGGCCGCTGCGCGACGACGACGGCAGCGTGCTGCATA
TCAAGCCAACCATCGCCTCGTCCAGCGGCGAGACCCTCCGGAGCCTGGCCCTGAACGGCCAGGGCATTGCCTGCCTGTCG
GACTTCATGACGCGCGACGATCGGCAAGACGGGCGGCTGGTGCAGCTGTTTGCGAAACAGACCCTCGATGTGCGCCAGTC
GATCAACGCGGTCTACTATCGCAACACCGCGTTGGCGTCCCGCATCACCTGCTTTGTCGAGTATGTGGTCAAGGTGCTTG
GCCAGAAGCCGTTCGAGAGCTAG

Upstream 100 bases:

>100_bases
GCAACTCCTATGAAGGAAACTCGAATGCACGCAGTTTGCCGCCCTTACCTTTGCGGATAAAGCCGTGTATAAGGGAAATT
CATTTGATTTGAAATCAATA

Downstream 100 bases:

>100_bases
TTTTCATATTTGATGCCAGATCAAAAGTATTTGACCGATACACGGCTTAATCTGCAAAGATGAACCGGGCAAACTGCGCT
CCATCGAAACCCATTCCCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKTTIDELLAFAAVVDTGSITAAAEQLKLTVSAASRTLGRLEEKLQTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEA
EEQIAARRLRPVGRLRVDAATPFMLHVIVPLLEGFRASYPEVELELNSNEGIIDLIEKRTDVAFRIGQLKDSTLHARPIG
TSRIRILASPGYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDDGSVLHIKPTIASSSGETLRSLALNGQGIACLS
DFMTRDDRQDGRLVQLFAKQTLDVRQSINAVYYRNTALASRITCFVEYVVKVLGQKPFES

Sequences:

>Translated_300_residues
MKTTIDELLAFAAVVDTGSITAAAEQLKLTVSAASRTLGRLEEKLQTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEA
EEQIAARRLRPVGRLRVDAATPFMLHVIVPLLEGFRASYPEVELELNSNEGIIDLIEKRTDVAFRIGQLKDSTLHARPIG
TSRIRILASPGYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDDGSVLHIKPTIASSSGETLRSLALNGQGIACLS
DFMTRDDRQDGRLVQLFAKQTLDVRQSINAVYYRNTALASRITCFVEYVVKVLGQKPFES
>Mature_300_residues
MKTTIDELLAFAAVVDTGSITAAAEQLKLTVSAASRTLGRLEEKLQTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEA
EEQIAARRLRPVGRLRVDAATPFMLHVIVPLLEGFRASYPEVELELNSNEGIIDLIEKRTDVAFRIGQLKDSTLHARPIG
TSRIRILASPGYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDDGSVLHIKPTIASSSGETLRSLALNGQGIACLS
DFMTRDDRQDGRLVQLFAKQTLDVRQSINAVYYRNTALASRITCFVEYVVKVLGQKPFES

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=46.8013468013468, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=31.4878892733564, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787128, Length=197, Percent_Identity=28.9340101522843, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787589, Length=264, Percent_Identity=28.4090909090909, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=27, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=29.1095890410959, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788481, Length=304, Percent_Identity=24.0131578947368, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786448, Length=178, Percent_Identity=28.0898876404494, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=267, Percent_Identity=28.4644194756554, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788297, Length=137, Percent_Identity=33.5766423357664, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082132, Length=304, Percent_Identity=24.0131578947368, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=175, Percent_Identity=29.7142857142857, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=209, Percent_Identity=26.7942583732057, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693105, Length=164, Percent_Identity=27.4390243902439, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33215; Mature: 33215

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTIDELLAFAAVVDTGSITAAAEQLKLTVSAASRTLGRLEEKLQTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGAAFLEHARAILAMVDEAEEQIAARRLRPVGRLRVDAATPFMLHVIVPLLEGFRASYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHHHHCCCC
EVELELNSNEGIIDLIEKRTDVAFRIGQLKDSTLHARPIGTSRIRILASPGYLKRHGTPT
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCCHHCCCCCC
KPAQLAQHALLGFTQPESLNDWPLRDDDGSVLHIKPTIASSSGETLRSLALNGQGIACLS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHH
DFMTRDDRQDGRLVQLFAKQTLDVRQSINAVYYRNTALASRITCFVEYVVKVLGQKPFES
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTTIDELLAFAAVVDTGSITAAAEQLKLTVSAASRTLGRLEEKLQTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGAAFLEHARAILAMVDEAEEQIAARRLRPVGRLRVDAATPFMLHVIVPLLEGFRASYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHHHHCCCC
EVELELNSNEGIIDLIEKRTDVAFRIGQLKDSTLHARPIGTSRIRILASPGYLKRHGTPT
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCCHHCCCCCC
KPAQLAQHALLGFTQPESLNDWPLRDDDGSVLHIKPTIASSSGETLRSLALNGQGIACLS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHH
DFMTRDDRQDGRLVQLFAKQTLDVRQSINAVYYRNTALASRITCFVEYVVKVLGQKPFES
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]