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The map label for this gene is proP [H]

Identifier: 116696008

GI number: 116696008

Start: 2332084

End: 2333394

Strand: Direct

Name: proP [H]

Synonym: H16_B2072

Alternate gene names: 116696008

Gene position: 2332084-2333394 (Clockwise)

Preceding gene: 116696005

Following gene: 116696010

Centisome position: 80.07

GC content: 66.97

Gene sequence:

>1311_bases
ATGTCTACCACCAGCAGTACCGCCGCAGGTATCGGCGCGCCGCGAGCCAGCGCGCGCCCGCTGACCGGCCAGGATTACAA
GACCCTTGCCCTTGCCGCGCTTGGCGGCGCCCTTGAGTTCTACGACTTCATCATCTTCGTTTTTTTCGCGACGGTCATCG
GGCAGCTGTTCTTCCCGCCGTCGGTGCCGGACTGGCTGCGCCAGTTGCAGACCTTCGGCATTTTCGCGGCCGGCTACCTG
GCCCGGCCGCTGGGCGGCATCATCATGGCGCACTTCGGTGACCTGCTCGGCCGCAAGAAGATGTTCACGCTGTCGATCCT
GCTGATGTCGGTGCCCACGCTGCTGATGGGCCTGATGCCCACCTACCAGTCGATCGGCCTGGCCGCGCCGGTGCTGCTGC
TGGTGCTGCGCATCCTGCAAGGCGCGGCCGTCGGCGGCGAAGTGCCCGGCGCCTGGGTGTTCGTATCGGAGCACGTGCCG
GCGCGCCATGTCGGCTATGCCTGCGGCACGCTGACCGCGGGGCTGACTGCCGGCATCCTGCTGGGTTCGCTGGTCGCCAC
CGGCATCAACAGCGTCTTCACCCCGGCCGAGCTGGCCGACCACGGCTGGCGCGTGCCGTTCCTGATCGGCGGCGTGTTCG
GCATCGCCTCGATGTACCTGCGGCGCTGGCTGCATGAGACGCCGGTCTTCGCCGAACTGCAGAAGCGCAAGGCGCTGGCC
GCGGAAATGCCGCTGAAGTCGGTGGTGCGCAACCACCGCGGCAGCGTCGCGGTGTCGATGCTGCTGACCTGGATGCTGTC
GGCCGGCATCGTGGTGGTGATCCTGATGACGCCCACCTTCCTGCAGAAGCTGTATGGCTTTGACGCGCGCACGGCACTGG
TCGCCAACAGCGTCGCCACGCTGTGCCTGACGATCGGCTGTGTCGTCTCCGGCGCATTAGCCGACCGCATCGGTGCGCGC
CGCACGCTGTTCGTCGGCGGCCTGCTGCTGGCCATCTGCGCCTATATGTTCTACACGACGATCTTCACCCGCCCCGACCT
GCTGCTGCCGCTCTATGCGCTGACCGGCTTCCTGGTCGGCACCATCGGCGCGGTGCCGTTCGTGCTGGTCAATGCGTTCC
CGGCGCAGGTGCGCTTCTCGGGCCTGTCATTCTCGTACAACGTGTCGTATGCGATCTTCGGCGGGCTGACGCCGATGGTC
GTCACGCTGATGCTGAAGCAGGATCCGCTGGCACCGGCCCACTATGTGGTCGCGCTGTGCGTGCTGGGCATGGTGACTGC
GCTGTTCGTCAAGGACCGCTCGCGCGGCTGA

Upstream 100 bases:

>100_bases
ACATCGGGCCTGCCTCCCCTGCGCTGTACCCTCCGGCCACACCCATCACGTATTCCGTGCTGCGCGCTTCGCATGCGATC
TCTTCCATGCGAGTCCATTC

Downstream 100 bases:

>100_bases
CGCGGCTGCTGCAAGGAAAAAGGGAGGGCAGGCGCCCTCCCTTTACTTGTGCGCGCTTGATGCGCATTGGTGCTGCCCGA
CTTCAACGGCGGTGCTGGCT

Product: MFS transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTTSSTAAGIGAPRASARPLTGQDYKTLALAALGGALEFYDFIIFVFFATVIGQLFFPPSVPDWLRQLQTFGIFAAGYL
ARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMPTYQSIGLAAPVLLLVLRILQGAAVGGEVPGAWVFVSEHVP
ARHVGYACGTLTAGLTAGILLGSLVATGINSVFTPAELADHGWRVPFLIGGVFGIASMYLRRWLHETPVFAELQKRKALA
AEMPLKSVVRNHRGSVAVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALVANSVATLCLTIGCVVSGALADRIGAR
RTLFVGGLLLAICAYMFYTTIFTRPDLLLPLYALTGFLVGTIGAVPFVLVNAFPAQVRFSGLSFSYNVSYAIFGGLTPMV
VTLMLKQDPLAPAHYVVALCVLGMVTALFVKDRSRG

Sequences:

>Translated_436_residues
MSTTSSTAAGIGAPRASARPLTGQDYKTLALAALGGALEFYDFIIFVFFATVIGQLFFPPSVPDWLRQLQTFGIFAAGYL
ARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMPTYQSIGLAAPVLLLVLRILQGAAVGGEVPGAWVFVSEHVP
ARHVGYACGTLTAGLTAGILLGSLVATGINSVFTPAELADHGWRVPFLIGGVFGIASMYLRRWLHETPVFAELQKRKALA
AEMPLKSVVRNHRGSVAVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALVANSVATLCLTIGCVVSGALADRIGAR
RTLFVGGLLLAICAYMFYTTIFTRPDLLLPLYALTGFLVGTIGAVPFVLVNAFPAQVRFSGLSFSYNVSYAIFGGLTPMV
VTLMLKQDPLAPAHYVVALCVLGMVTALFVKDRSRG
>Mature_435_residues
STTSSTAAGIGAPRASARPLTGQDYKTLALAALGGALEFYDFIIFVFFATVIGQLFFPPSVPDWLRQLQTFGIFAAGYLA
RPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMPTYQSIGLAAPVLLLVLRILQGAAVGGEVPGAWVFVSEHVPA
RHVGYACGTLTAGLTAGILLGSLVATGINSVFTPAELADHGWRVPFLIGGVFGIASMYLRRWLHETPVFAELQKRKALAA
EMPLKSVVRNHRGSVAVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALVANSVATLCLTIGCVVSGALADRIGARR
TLFVGGLLLAICAYMFYTTIFTRPDLLLPLYALTGFLVGTIGAVPFVLVNAFPAQVRFSGLSFSYNVSYAIFGGLTPMVV
TLMLKQDPLAPAHYVVALCVLGMVTALFVKDRSRG

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=450, Percent_Identity=30, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=29.4117647058824, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=32.7751196172249, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789941, Length=434, Percent_Identity=28.8018433179723, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082231, Length=468, Percent_Identity=23.0769230769231, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=23.5616438356164, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46490; Mature: 46359

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STTSSTAAGIGAPRASARPLTGQDYKTLALAALGGALEFYDFIIFVFFATVIGQLFFPP
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVPDWLRQLQTFGIFAAGYLARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TYQSIGLAAPVLLLVLRILQGAAVGGEVPGAWVFVSEHVPARHVGYACGTLTAGLTAGIL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
LGSLVATGINSVFTPAELADHGWRVPFLIGGVFGIASMYLRRWLHETPVFAELQKRKALA
HHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AEMPLKSVVRNHRGSVAVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALVANSVAT
HHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHH
LCLTIGCVVSGALADRIGARRTLFVGGLLLAICAYMFYTTIFTRPDLLLPLYALTGFLVG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TIGAVPFVLVNAFPAQVRFSGLSFSYNVSYAIFGGLTPMVVTLMLKQDPLAPAHYVVALC
HHHHHHHHHHHCCCHHEEECCCEEEEECHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
VLGMVTALFVKDRSRG
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]