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The map label for this gene is gatA [H]

Identifier: 116695949

GI number: 116695949

Start: 2270646

End: 2271950

Strand: Direct

Name: gatA [H]

Synonym: H16_B2013

Alternate gene names: 116695949

Gene position: 2270646-2271950 (Clockwise)

Preceding gene: 116695948

Following gene: 116695950

Centisome position: 77.96

GC content: 70.96

Gene sequence:

>1305_bases
ATGGACGACTTCCTCGATCTTCCGGTCCGCACGCTGGCGGCGATGGTGTCGCGGCGCGAACTGAGCGCCGAAGCGGTGGT
CCGTGCGGCACTGGCCCGCATTGCGGCGCAAGACGCAGGCATTCTGGCCTGGCAGCACGTGGGTGGCGACACCGCCGTGG
CGCAGGCGCGGCGGCTGGATGCCGGCGGCGCGTCAGGCGCGTTGCCGGGCGTCCCGCTTGGCGCCAAGGACCTGATGGAT
ACAGCCGACATGCCGACCACTTATGGTTCGCCGATATACGCCGGACATCGGCCGGGCGTGGATGCGGCGGCGGTGGCACT
GGCCCGATCGGCGGGCGCGGTCGTGGTCGGCAAGACCGTCACCACCGAATTCGCCACGTTTCAGCCGGGGCCGACCCGCA
ACCCGCGCGCGCCGGCGGACAGGCCCCGCACCCCCGGCGGCTCGTCGAGCGGATCGGCCGCCGCCGTCGCCGCAGGCATG
GTGCCGCTGGCCTTCGGCACACAGACCGCGGGATCGATTGTGCGGCCGGCCGCCTATTGCGGCGTGGTGGGCTACAAGCC
CACGCATGGCACACTGCCGCTGGCCGGTATCAAGCCGCTGGCACCAAGCCTGGATACGGTGGGCGTGCTGGCCCGCCGCG
TGGACGACGCGGCCTTCTTCATCGGCACCCTGGCGCGCGATGCCGCGCTAGCGGACGCGTCGACGCTACGCCACGCGCCG
CTGCGCATCGGCATTTGCCGCACGCCGCATTGGGAACGTGCTGGTGCCGATACACAAGCCGCACTGGACACCGCGATCCG
TGCGCTCGAACGCATCGGTGGCACGGTGCGCGACTTCAACCTGCCGCAGGCTTGCCACGGGCTGACTGAAGCCCAACTGG
ACATCATGGCATTCGAGGCACTCGCCGCATTCGCGCCGGAAGCACGCGAGCACGCAGAAGACTTCAGCGCGGCTTTCGCG
CGCCAGCTCGAAGCGGGCCGCGCCGTCACCGGCCAGCGCTTCCATGTCGCGCTGGCATACGCGGAAGCGGCCCGCACGGC
GTTCAACACGGCGATGGCAGGATTCGACGTCGTGCTGGCCCCGAGCGCCGAAGGCGAAGCGCCTGCTGGCCTTGGCGCCA
CCGGCGATCCGATTTTCTGCCGCATGTGGTCGCTGCTGGGCACCCCATGCGTCCATGTGCCGACGGGCACCGGCGCCACC
GGCATGCCGGTTGGCGTGACCGTGACGGGGCCGCGCCATGCCGATGCCCGCGTGCTCGGCATGGCTCATCGCCTTGAGCA
GGCGCTTGGCGCCAGCCTTGCCTGA

Upstream 100 bases:

>100_bases
GCTGACCCAGGGCCGTGTCGTGGCCCAGGGCACCGCGCAGGCGCTGCGCGAGAACGATGAGGTTCGCGCAGCCTATTTCG
GCTGATCCGGAGAACATGCG

Downstream 100 bases:

>100_bases
TATCTGGAGACCAAATTGACCGTATCGAACCCGCCCCCCCTCGCCAACGCACAAGCCATTGCCGAACGCGCACAGGATCA
TGTGGGCGCCGGGCGCCTGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDDFLDLPVRTLAAMVSRRELSAEAVVRAALARIAAQDAGILAWQHVGGDTAVAQARRLDAGGASGALPGVPLGAKDLMD
TADMPTTYGSPIYAGHRPGVDAAAVALARSAGAVVVGKTVTTEFATFQPGPTRNPRAPADRPRTPGGSSSGSAAAVAAGM
VPLAFGTQTAGSIVRPAAYCGVVGYKPTHGTLPLAGIKPLAPSLDTVGVLARRVDDAAFFIGTLARDAALADASTLRHAP
LRIGICRTPHWERAGADTQAALDTAIRALERIGGTVRDFNLPQACHGLTEAQLDIMAFEALAAFAPEAREHAEDFSAAFA
RQLEAGRAVTGQRFHVALAYAEAARTAFNTAMAGFDVVLAPSAEGEAPAGLGATGDPIFCRMWSLLGTPCVHVPTGTGAT
GMPVGVTVTGPRHADARVLGMAHRLEQALGASLA

Sequences:

>Translated_434_residues
MDDFLDLPVRTLAAMVSRRELSAEAVVRAALARIAAQDAGILAWQHVGGDTAVAQARRLDAGGASGALPGVPLGAKDLMD
TADMPTTYGSPIYAGHRPGVDAAAVALARSAGAVVVGKTVTTEFATFQPGPTRNPRAPADRPRTPGGSSSGSAAAVAAGM
VPLAFGTQTAGSIVRPAAYCGVVGYKPTHGTLPLAGIKPLAPSLDTVGVLARRVDDAAFFIGTLARDAALADASTLRHAP
LRIGICRTPHWERAGADTQAALDTAIRALERIGGTVRDFNLPQACHGLTEAQLDIMAFEALAAFAPEAREHAEDFSAAFA
RQLEAGRAVTGQRFHVALAYAEAARTAFNTAMAGFDVVLAPSAEGEAPAGLGATGDPIFCRMWSLLGTPCVHVPTGTGAT
GMPVGVTVTGPRHADARVLGMAHRLEQALGASLA
>Mature_434_residues
MDDFLDLPVRTLAAMVSRRELSAEAVVRAALARIAAQDAGILAWQHVGGDTAVAQARRLDAGGASGALPGVPLGAKDLMD
TADMPTTYGSPIYAGHRPGVDAAAVALARSAGAVVVGKTVTTEFATFQPGPTRNPRAPADRPRTPGGSSSGSAAAVAAGM
VPLAFGTQTAGSIVRPAAYCGVVGYKPTHGTLPLAGIKPLAPSLDTVGVLARRVDDAAFFIGTLARDAALADASTLRHAP
LRIGICRTPHWERAGADTQAALDTAIRALERIGGTVRDFNLPQACHGLTEAQLDIMAFEALAAFAPEAREHAEDFSAAFA
RQLEAGRAVTGQRFHVALAYAEAARTAFNTAMAGFDVVLAPSAEGEAPAGLGATGDPIFCRMWSLLGTPCVHVPTGTGAT
GMPVGVTVTGPRHADARVLGMAHRLEQALGASLA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=25.1485148514851, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI195972892, Length=204, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=429, Percent_Identity=26.3403263403263, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=25.2609603340292, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=25.4966887417219, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=384, Percent_Identity=27.6041666666667, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648113, Length=317, Percent_Identity=33.4384858044164, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078093, Length=484, Percent_Identity=23.3471074380165, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45550774, Length=481, Percent_Identity=21.8295218295218, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=481, Percent_Identity=22.037422037422, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648437, Length=481, Percent_Identity=22.037422037422, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356731, Length=203, Percent_Identity=32.0197044334975, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 44339; Mature: 44339

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDFLDLPVRTLAAMVSRRELSAEAVVRAALARIAAQDAGILAWQHVGGDTAVAQARRLD
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCC
AGGASGALPGVPLGAKDLMDTADMPTTYGSPIYAGHRPGVDAAAVALARSAGAVVVGKTV
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCEE
TTEFATFQPGPTRNPRAPADRPRTPGGSSSGSAAAVAAGMVPLAFGTQTAGSIVRPAAYC
ECHHEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCHHHCHHHHH
GVVGYKPTHGTLPLAGIKPLAPSLDTVGVLARRVDDAAFFIGTLARDAALADASTLRHAP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LRIGICRTPHWERAGADTQAALDTAIRALERIGGTVRDFNLPQACHGLTEAQLDIMAFEA
EEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCEECCCCCHHHCCCHHHHHHHHHHHH
LAAFAPEAREHAEDFSAAFARQLEAGRAVTGQRFHVALAYAEAARTAFNTAMAGFDVVLA
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEE
PSAEGEAPAGLGATGDPIFCRMWSLLGTPCVHVPTGTGATGMPVGVTVTGPRHADARVLG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHH
MAHRLEQALGASLA
HHHHHHHHHCCCCC
>Mature Secondary Structure
MDDFLDLPVRTLAAMVSRRELSAEAVVRAALARIAAQDAGILAWQHVGGDTAVAQARRLD
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCC
AGGASGALPGVPLGAKDLMDTADMPTTYGSPIYAGHRPGVDAAAVALARSAGAVVVGKTV
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCEE
TTEFATFQPGPTRNPRAPADRPRTPGGSSSGSAAAVAAGMVPLAFGTQTAGSIVRPAAYC
ECHHEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCHHHCHHHHH
GVVGYKPTHGTLPLAGIKPLAPSLDTVGVLARRVDDAAFFIGTLARDAALADASTLRHAP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LRIGICRTPHWERAGADTQAALDTAIRALERIGGTVRDFNLPQACHGLTEAQLDIMAFEA
EEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCEECCCCCHHHCCCHHHHHHHHHHHH
LAAFAPEAREHAEDFSAAFARQLEAGRAVTGQRFHVALAYAEAARTAFNTAMAGFDVVLA
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEE
PSAEGEAPAGLGATGDPIFCRMWSLLGTPCVHVPTGTGATGMPVGVTVTGPRHADARVLG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHH
MAHRLEQALGASLA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA