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The map label for this gene is mscS4 [H]

Identifier: 116695791

GI number: 116695791

Start: 2095550

End: 2098003

Strand: Direct

Name: mscS4 [H]

Synonym: H16_B1855

Alternate gene names: 116695791

Gene position: 2095550-2098003 (Clockwise)

Preceding gene: 116695789

Following gene: 116695792

Centisome position: 71.95

GC content: 68.34

Gene sequence:

>2454_bases
ATGAGCAAAGTTTCACGGCTCGCACTGGGCCTTGCGCTCGCTGCGCTATGCCACCTGACGCCGGCCGCACTGGCCGCCCC
GGCATCAGAAGCGGCTTCCGAGGCCGGCGCCCAGCAGGCGCCCGCCATCAGCCACGGCGAGGCCGCCGCCGAACTCAAGC
GCCTGCAGACCGAGCAGGACCGCATCAAGCAGCAGGCCTCCGACCCGGAAGGCAACACCAGGCTGCATGAACTCGAAGAA
ACGCTGCAGAAGCTCGATGCCGACGTCGACAAGCTGTCTTCGGCGCTGACGCCGCAGCGGGCGCAGCTGCAGGCGCAGCT
GGACGTGCTGGGCCCGCCACCGGCGGCCGCCACCACCAAGGAAGCGCCGGCGGTGGTCAGGCAGCGCGCCGATCTGAATG
CGCGCCGTGCCCAGCTTGACGCACAACTCAAGCAGGCAGGAGAGAGCAAGGCGAACATCGCCAACCTGAGCGACCAGCTG
GCGCGGCTGCAGCGCGGCCAGATGAAGGACCAGCTCGCGCTGCGCTCCGAGAGCATCCTGAACCCGCAGTTCTGGAAGCC
GCTGGTCAGCCCGACACAGGAAGACCAGGCGCGCCTGGCCACCTTTACCGACCAGGTGGTGCCGATGGCGCAACTGGCCT
GGCAGCCGTCACAGCGCGCCGCCACCATCGCCTTGCTGTTGCTGGCCCTGGCGGTATGGACCCTGGGCCGGCGCCTGGCG
GAACGGGCACTGGCGTGGTTCTGCCTGCACAAGCTGCCGGAGACGCGCCTGCGCCGCAGCGCCCTGGCACTGTCCACCAC
GCTGGCCACCGTGTGCACCACCGGGCTGGCGGTGCAGCTGGTCTACAACGCCTTCACGCGCAGCTATGAACTGCCGCCGG
ACCTGGTCGAGCTGTACGGCCAGCTGCTCAAGCTGACGCTGACCAGCGCGCTGATCGCGGGCCTGGGGCGCGCGCTGCTG
TGCACGCGCCATCCCTCCTGGCGCCTGCCGGCGCTGGCCGATCCGGTGGCGCTGGCGATGAAGCCGTTCCCCGCCGTGCT
GGCCGGCCTGTTGCTGCTGGCCGGCACGCTCGAGCAGCTGAACCGCATTGCCGATACCAGCGTGCAGGTCACGCTGCTGG
GACGCGGGCTGGTCTCGCTGGTGGTGGTGCTGACCATCGGCGCCGCGCTGCTGCGCGCCAACCGCGTGCGCAACCTGCTG
GCGGCCGCCGGCGAGCGGCCCGAGGCGCGCGCCACGCTGGCGGGCATGATCCACGCCGGCGTGTCGGTCATCGTGCTGGT
GTCGGTGGTGGCGCTGCTGGCCGGCTACATCAGCTTTGCGCGCTTCCTGACCTACGAGCTGGTCTGGTTCGACATCGTGC
TGTGCAGCCTGTACCTGCTGACCCAGGTCACGCGCGACGTCTGCGAAAGCCTGCTCTCGACCAACTACGCCAGCGGCCGT
GTCATCAAGCAGCTGTTCGGCGTGGACGATTCGCACCTGGAGCAGGCCTCCACCATCCTGTCAGGCATCGGCGCCAGCGT
GCTGCTGCTGGTGGCGGTGCTGGCGCTGCTGACCGGCGGCTTCGGCACCACACCCGCGGACCTGCTCAACAGCCTGGTAA
TGGTGCTGGGCGGCGAGAAGCTGCACAGCCTCAACATCATGCCCGACCGCATCCTGAATGCGCTGGTCGCTCTCGGGGTC
GGCATCTGGCTGCTGCGCTCGGTGCGGCGCTGGCTCGATGGAGAACTGCTGCCCAAGGTCTGCAAGGAGCCGGGCCTGCG
CGCCTCGCTGATTACGCTGTACAGCAATGTCGGCTACGTGTTGCTGGTGCTGCTGGTGCTGTCGCTGCTGGGCGTGCGCT
GGGACAACCTGGCGTGGATCGTCAGCGCGCTGTCGGTGGGTATCGGCTTCGGCCTGCAGGAGATCGTGAAGAACTTCGTC
TCCGGCCTGATCCTGCTGACCGAGCGTCCGGTCAAGGTGGGCGACATGGTCAGCATTGCCGGCGTGGAGGGCGATATCCG
CCGCATCAACGTGCGCGCTACCGAAATCCAGCTGGGCGACCGCTCCACCGTGATCGTGCCCAACTCGCAGCTGATCTCGC
AGAACCTGCGCAACGTGACCATGAGCAACAGCACCCAGGGAGTGGCGTCGCTGCAACTTACCTTCCCCCTGAATACCGAC
CCCGAACAGCTGCGCGACCTGCTGCTGGACGTCTACCGCGAGAACGAGAGCATCCTGGACGTGCCGGCACCCTCGGTCAT
GTTCAGCCAGCTGGCGCCCAACGGCATCACGCTGTCGGTGACGGGCTATGTCGGCAGCCCGCGCATCGCCGCCAACACGC
GCAGCGACCTGTTGTTCGAAATCCTCAAGCGCCTGCGCGCCGCGGACATCTCGCTGTCGGTGCCGCAATCGCTGCGGCTG
GAAAACATGCCGCCATTCGGGCAGGAGCCCGAGCGCGCGCTGGCCGCCGGCTGA

Upstream 100 bases:

>100_bases
CCGTTCCCGCATCCCATCGCCGCCGCACCCGACGCGTGCCAGCGCGATGCCGTTGCCGATGCCGGGGCCGCCGCAAGTCA
CCATCCGACGAGATTCCGCC

Downstream 100 bases:

>100_bases
CCGGCATTAAAGCAGCCGTTACAACCCTAACAAGTCGCCGGCCGCGCAGAAACACCTTGAGGGGCGCTTTGCGTGCATCA
TGAATGCACACAAGCTGCAT

Product: small-conductance mechano-sensitive channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 817; Mature: 816

Protein sequence:

>817_residues
MSKVSRLALGLALAALCHLTPAALAAPASEAASEAGAQQAPAISHGEAAAELKRLQTEQDRIKQQASDPEGNTRLHELEE
TLQKLDADVDKLSSALTPQRAQLQAQLDVLGPPPAAATTKEAPAVVRQRADLNARRAQLDAQLKQAGESKANIANLSDQL
ARLQRGQMKDQLALRSESILNPQFWKPLVSPTQEDQARLATFTDQVVPMAQLAWQPSQRAATIALLLLALAVWTLGRRLA
ERALAWFCLHKLPETRLRRSALALSTTLATVCTTGLAVQLVYNAFTRSYELPPDLVELYGQLLKLTLTSALIAGLGRALL
CTRHPSWRLPALADPVALAMKPFPAVLAGLLLLAGTLEQLNRIADTSVQVTLLGRGLVSLVVVLTIGAALLRANRVRNLL
AAAGERPEARATLAGMIHAGVSVIVLVSVVALLAGYISFARFLTYELVWFDIVLCSLYLLTQVTRDVCESLLSTNYASGR
VIKQLFGVDDSHLEQASTILSGIGASVLLLVAVLALLTGGFGTTPADLLNSLVMVLGGEKLHSLNIMPDRILNALVALGV
GIWLLRSVRRWLDGELLPKVCKEPGLRASLITLYSNVGYVLLVLLVLSLLGVRWDNLAWIVSALSVGIGFGLQEIVKNFV
SGLILLTERPVKVGDMVSIAGVEGDIRRINVRATEIQLGDRSTVIVPNSQLISQNLRNVTMSNSTQGVASLQLTFPLNTD
PEQLRDLLLDVYRENESILDVPAPSVMFSQLAPNGITLSVTGYVGSPRIAANTRSDLLFEILKRLRAADISLSVPQSLRL
ENMPPFGQEPERALAAG

Sequences:

>Translated_817_residues
MSKVSRLALGLALAALCHLTPAALAAPASEAASEAGAQQAPAISHGEAAAELKRLQTEQDRIKQQASDPEGNTRLHELEE
TLQKLDADVDKLSSALTPQRAQLQAQLDVLGPPPAAATTKEAPAVVRQRADLNARRAQLDAQLKQAGESKANIANLSDQL
ARLQRGQMKDQLALRSESILNPQFWKPLVSPTQEDQARLATFTDQVVPMAQLAWQPSQRAATIALLLLALAVWTLGRRLA
ERALAWFCLHKLPETRLRRSALALSTTLATVCTTGLAVQLVYNAFTRSYELPPDLVELYGQLLKLTLTSALIAGLGRALL
CTRHPSWRLPALADPVALAMKPFPAVLAGLLLLAGTLEQLNRIADTSVQVTLLGRGLVSLVVVLTIGAALLRANRVRNLL
AAAGERPEARATLAGMIHAGVSVIVLVSVVALLAGYISFARFLTYELVWFDIVLCSLYLLTQVTRDVCESLLSTNYASGR
VIKQLFGVDDSHLEQASTILSGIGASVLLLVAVLALLTGGFGTTPADLLNSLVMVLGGEKLHSLNIMPDRILNALVALGV
GIWLLRSVRRWLDGELLPKVCKEPGLRASLITLYSNVGYVLLVLLVLSLLGVRWDNLAWIVSALSVGIGFGLQEIVKNFV
SGLILLTERPVKVGDMVSIAGVEGDIRRINVRATEIQLGDRSTVIVPNSQLISQNLRNVTMSNSTQGVASLQLTFPLNTD
PEQLRDLLLDVYRENESILDVPAPSVMFSQLAPNGITLSVTGYVGSPRIAANTRSDLLFEILKRLRAADISLSVPQSLRL
ENMPPFGQEPERALAAG
>Mature_816_residues
SKVSRLALGLALAALCHLTPAALAAPASEAASEAGAQQAPAISHGEAAAELKRLQTEQDRIKQQASDPEGNTRLHELEET
LQKLDADVDKLSSALTPQRAQLQAQLDVLGPPPAAATTKEAPAVVRQRADLNARRAQLDAQLKQAGESKANIANLSDQLA
RLQRGQMKDQLALRSESILNPQFWKPLVSPTQEDQARLATFTDQVVPMAQLAWQPSQRAATIALLLLALAVWTLGRRLAE
RALAWFCLHKLPETRLRRSALALSTTLATVCTTGLAVQLVYNAFTRSYELPPDLVELYGQLLKLTLTSALIAGLGRALLC
TRHPSWRLPALADPVALAMKPFPAVLAGLLLLAGTLEQLNRIADTSVQVTLLGRGLVSLVVVLTIGAALLRANRVRNLLA
AAGERPEARATLAGMIHAGVSVIVLVSVVALLAGYISFARFLTYELVWFDIVLCSLYLLTQVTRDVCESLLSTNYASGRV
IKQLFGVDDSHLEQASTILSGIGASVLLLVAVLALLTGGFGTTPADLLNSLVMVLGGEKLHSLNIMPDRILNALVALGVG
IWLLRSVRRWLDGELLPKVCKEPGLRASLITLYSNVGYVLLVLLVLSLLGVRWDNLAWIVSALSVGIGFGLQEIVKNFVS
GLILLTERPVKVGDMVSIAGVEGDIRRINVRATEIQLGDRSTVIVPNSQLISQNLRNVTMSNSTQGVASLQLTFPLNTDP
EQLRDLLLDVYRENESILDVPAPSVMFSQLAPNGITLSVTGYVGSPRIAANTRSDLLFEILKRLRAADISLSVPQSLRLE
NMPPFGQEPERALAAG

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=252, Percent_Identity=32.9365079365079, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI2367355, Length=426, Percent_Identity=27.2300469483568, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789291, Length=190, Percent_Identity=23.1578947368421, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 87898; Mature: 87766

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVSRLALGLALAALCHLTPAALAAPASEAASEAGAQQAPAISHGEAAAELKRLQTEQD
CCHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
RIKQQASDPEGNTRLHELEETLQKLDADVDKLSSALTPQRAQLQAQLDVLGPPPAAATTK
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
EAPAVVRQRADLNARRAQLDAQLKQAGESKANIANLSDQLARLQRGQMKDQLALRSESIL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPQFWKPLVSPTQEDQARLATFTDQVVPMAQLAWQPSQRAATIALLLLALAVWTLGRRLA
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERALAWFCLHKLPETRLRRSALALSTTLATVCTTGLAVQLVYNAFTRSYELPPDLVELYG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QLLKLTLTSALIAGLGRALLCTRHPSWRLPALADPVALAMKPFPAVLAGLLLLAGTLEQL
HHHHHHHHHHHHHHHCHHHEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
NRIADTSVQVTLLGRGLVSLVVVLTIGAALLRANRVRNLLAAAGERPEARATLAGMIHAG
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VSVIVLVSVVALLAGYISFARFLTYELVWFDIVLCSLYLLTQVTRDVCESLLSTNYASGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VIKQLFGVDDSHLEQASTILSGIGASVLLLVAVLALLTGGFGTTPADLLNSLVMVLGGEK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
LHSLNIMPDRILNALVALGVGIWLLRSVRRWLDGELLPKVCKEPGLRASLITLYSNVGYV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHH
LLVLLVLSLLGVRWDNLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSIA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEE
GVEGDIRRINVRATEIQLGDRSTVIVPNSQLISQNLRNVTMSNSTQGVASLQLTFPLNTD
CCCCCCEEEEEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCC
PEQLRDLLLDVYRENESILDVPAPSVMFSQLAPNGITLSVTGYVGSPRIAANTRSDLLFE
HHHHHHHHHHHHHCCCCEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHH
ILKRLRAADISLSVPQSLRLENMPPFGQEPERALAAG
HHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SKVSRLALGLALAALCHLTPAALAAPASEAASEAGAQQAPAISHGEAAAELKRLQTEQD
CHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
RIKQQASDPEGNTRLHELEETLQKLDADVDKLSSALTPQRAQLQAQLDVLGPPPAAATTK
HHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCC
EAPAVVRQRADLNARRAQLDAQLKQAGESKANIANLSDQLARLQRGQMKDQLALRSESIL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPQFWKPLVSPTQEDQARLATFTDQVVPMAQLAWQPSQRAATIALLLLALAVWTLGRRLA
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERALAWFCLHKLPETRLRRSALALSTTLATVCTTGLAVQLVYNAFTRSYELPPDLVELYG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QLLKLTLTSALIAGLGRALLCTRHPSWRLPALADPVALAMKPFPAVLAGLLLLAGTLEQL
HHHHHHHHHHHHHHHCHHHEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
NRIADTSVQVTLLGRGLVSLVVVLTIGAALLRANRVRNLLAAAGERPEARATLAGMIHAG
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VSVIVLVSVVALLAGYISFARFLTYELVWFDIVLCSLYLLTQVTRDVCESLLSTNYASGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VIKQLFGVDDSHLEQASTILSGIGASVLLLVAVLALLTGGFGTTPADLLNSLVMVLGGEK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
LHSLNIMPDRILNALVALGVGIWLLRSVRRWLDGELLPKVCKEPGLRASLITLYSNVGYV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHH
LLVLLVLSLLGVRWDNLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSIA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEE
GVEGDIRRINVRATEIQLGDRSTVIVPNSQLISQNLRNVTMSNSTQGVASLQLTFPLNTD
CCCCCCEEEEEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCC
PEQLRDLLLDVYRENESILDVPAPSVMFSQLAPNGITLSVTGYVGSPRIAANTRSDLLFE
HHHHHHHHHHHHHCCCCEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHH
ILKRLRAADISLSVPQSLRLENMPPFGQEPERALAAG
HHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]