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The map label for this gene is 116695699

Identifier: 116695699

GI number: 116695699

Start: 2003792

End: 2004916

Strand: Direct

Name: 116695699

Synonym: H16_B1763

Alternate gene names: NA

Gene position: 2003792-2004916 (Clockwise)

Preceding gene: 116695697

Following gene: 116695700

Centisome position: 68.8

GC content: 68.53

Gene sequence:

>1125_bases
ATGGACACTTCCAGGCCTTCCAGCCAGCGCCGCAACCTTTGTTCGATCCTGGGCATTTCCACGCCCATCATCCAGGCGCC
CATGGCCGGTGTCAGCACAGCGGCCATGGCTGCAGCCGTGTCAGAGGCCGGCGGGCTGGGCTCGCTCGGCGTCGGGGCAA
TGGATGCCGAAGCGGCGCGAAAGACAATCCGCGAGACACGAGCGCTGACCGGCAAGCCATTCAACGTCAACCTGTTCTGC
CACGCCCCCGCCAAGGCCGATGAGGTGCGCGAGCAGGCCTGGCTCAGCTACCTTGCCGCCCAGTTTGCCGAGCACGGCGC
GACCACACCCGCCAGCTTGCGCGAGATCTACAGGAGTTTCGTGGCCGACGACGCCATGTTCCAGATGCTGCTGGAAGAAA
AGCCAGCGGTTGTGAGCTTCCATTTTGGCCTTCCCGCCAAGCAGAAAATTGACGCGCTGCGCGCGGCCGGCATTGTGCTG
CTGGCCAGTGCAACGTCCCTGCATGAGGCCCGCCAGATAGAGGCCGCCGGCGTGGACGCCATCGTCGCGCAAGGCATTGA
AGCTGGCGGACACCGCGGCGCCTTCGACACCGATGGCTACGACGAAGGCCTCGGCACGCTGGCTCTGGTGCGTCTTCTGG
TGCTGGGCACGAAACTTCCGGTGATTGCCTCGGGCGGCATCATGGACGGGGCAGGCATTGCCGCGGTACTGGCTCTCGGG
GCAGAAGCCGCCCAGCTTGGCACCGCATTCGTCGCCTGCCCGGAGTCATCTGCCGATGCCGCCTATCGCGCGGCGCTGAC
CGCGCCCCAGCCGCGCGCGACCACGCTCACCCGCGCCATATCGGGCCGCGCGGCCAGGGGCTTCACCAACCGGCTCACGG
CACTCGGCGCCGCCGCCGACGCACCTCCGGTGCCGGACTACCCGATCGCCTATGACGCGGGAAAGGCGCTTCACGCCGCT
GCCAAGGCCGCGGGGAGCGCTGAGTACGCGGCGCAGTGGGCCGGGCAGGCAGCGCCGCTGGCCAGGTCCCTGCCGGCCGC
GGAGCTGGTGGCGCAACTCAGGTCGGAGCTCAGGCAAGCGATCCTGCGCATGGCGACGTTTGCGGACCCGCAGGACGCAG
CCTGA

Upstream 100 bases:

>100_bases
CGCCGCGAGATTTCATGGCATTCTCGATACCCTCCGACACAGCCTCGCCTTCGAAAATTTCCCGAGCGGAATCATGCACA
TCAAAGCGAAAGAGACTCTC

Downstream 100 bases:

>100_bases
GCGTATCTATAACAATTATTTGAATGTACTGACTGCCGGCTCTACGATGGCGCAACCCAACGTCGCCACTCCCACGAGGC
CCCATGCATATCGCCATCCT

Product: 2-nitropropane dioxygenase

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDTSRPSSQRRNLCSILGISTPIIQAPMAGVSTAAMAAAVSEAGGLGSLGVGAMDAEAARKTIRETRALTGKPFNVNLFC
HAPAKADEVREQAWLSYLAAQFAEHGATTPASLREIYRSFVADDAMFQMLLEEKPAVVSFHFGLPAKQKIDALRAAGIVL
LASATSLHEARQIEAAGVDAIVAQGIEAGGHRGAFDTDGYDEGLGTLALVRLLVLGTKLPVIASGGIMDGAGIAAVLALG
AEAAQLGTAFVACPESSADAAYRAALTAPQPRATTLTRAISGRAARGFTNRLTALGAAADAPPVPDYPIAYDAGKALHAA
AKAAGSAEYAAQWAGQAAPLARSLPAAELVAQLRSELRQAILRMATFADPQDAA

Sequences:

>Translated_374_residues
MDTSRPSSQRRNLCSILGISTPIIQAPMAGVSTAAMAAAVSEAGGLGSLGVGAMDAEAARKTIRETRALTGKPFNVNLFC
HAPAKADEVREQAWLSYLAAQFAEHGATTPASLREIYRSFVADDAMFQMLLEEKPAVVSFHFGLPAKQKIDALRAAGIVL
LASATSLHEARQIEAAGVDAIVAQGIEAGGHRGAFDTDGYDEGLGTLALVRLLVLGTKLPVIASGGIMDGAGIAAVLALG
AEAAQLGTAFVACPESSADAAYRAALTAPQPRATTLTRAISGRAARGFTNRLTALGAAADAPPVPDYPIAYDAGKALHAA
AKAAGSAEYAAQWAGQAAPLARSLPAAELVAQLRSELRQAILRMATFADPQDAA
>Mature_374_residues
MDTSRPSSQRRNLCSILGISTPIIQAPMAGVSTAAMAAAVSEAGGLGSLGVGAMDAEAARKTIRETRALTGKPFNVNLFC
HAPAKADEVREQAWLSYLAAQFAEHGATTPASLREIYRSFVADDAMFQMLLEEKPAVVSFHFGLPAKQKIDALRAAGIVL
LASATSLHEARQIEAAGVDAIVAQGIEAGGHRGAFDTDGYDEGLGTLALVRLLVLGTKLPVIASGGIMDGAGIAAVLALG
AEAAQLGTAFVACPESSADAAYRAALTAPQPRATTLTRAISGRAARGFTNRLTALGAAADAPPVPDYPIAYDAGKALHAA
AKAAGSAEYAAQWAGQAAPLARSLPAAELVAQLRSELRQAILRMATFADPQDAA

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=360, Percent_Identity=32.2222222222222, Blast_Score=162, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 38339; Mature: 38339

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSRPSSQRRNLCSILGISTPIIQAPMAGVSTAAMAAAVSEAGGLGSLGVGAMDAEAAR
CCCCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KTIRETRALTGKPFNVNLFCHAPAKADEVREQAWLSYLAAQFAEHGATTPASLREIYRSF
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VADDAMFQMLLEEKPAVVSFHFGLPAKQKIDALRAAGIVLLASATSLHEARQIEAAGVDA
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCHHH
IVAQGIEAGGHRGAFDTDGYDEGLGTLALVRLLVLGTKLPVIASGGIMDGAGIAAVLALG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHC
AEAAQLGTAFVACPESSADAAYRAALTAPQPRATTLTRAISGRAARGFTNRLTALGAAAD
HHHHHHCCEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHCCCCC
APPVPDYPIAYDAGKALHAAAKAAGSAEYAAQWAGQAAPLARSLPAAELVAQLRSELRQA
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHH
ILRMATFADPQDAA
HHHHHHCCCCCCCC
>Mature Secondary Structure
MDTSRPSSQRRNLCSILGISTPIIQAPMAGVSTAAMAAAVSEAGGLGSLGVGAMDAEAAR
CCCCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KTIRETRALTGKPFNVNLFCHAPAKADEVREQAWLSYLAAQFAEHGATTPASLREIYRSF
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VADDAMFQMLLEEKPAVVSFHFGLPAKQKIDALRAAGIVLLASATSLHEARQIEAAGVDA
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCHHH
IVAQGIEAGGHRGAFDTDGYDEGLGTLALVRLLVLGTKLPVIASGGIMDGAGIAAVLALG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHC
AEAAQLGTAFVACPESSADAAYRAALTAPQPRATTLTRAISGRAARGFTNRLTALGAAAD
HHHHHHCCEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHCCCCC
APPVPDYPIAYDAGKALHAAAKAAGSAEYAAQWAGQAAPLARSLPAAELVAQLRSELRQA
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHH
ILRMATFADPQDAA
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA