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The map label for this gene is pulA [H]

Identifier: 116695501

GI number: 116695501

Start: 1754188

End: 1756422

Strand: Direct

Name: pulA [H]

Synonym: H16_B1560

Alternate gene names: 116695501

Gene position: 1754188-1756422 (Clockwise)

Preceding gene: 116695500

Following gene: 116695502

Centisome position: 60.23

GC content: 70.43

Gene sequence:

>2235_bases
ATGAGCCGACAGTTCGCGGACCGGCTGCTGCCGGGCAAGCCGTATCCCCTCGGGGCCCACTGGGACGGCCTGGGTGTCAA
TTTCGCGGTGTTCTCGGCCCATGCCTCGCGCATCGACCTGTGCCTGTTCTCCGACAACGGGCGCAAGGAGCTGGCGCGCC
TGCAGCTGCCGGAACGCACCGACGAGATCTGGCACGGCTACCTGCCCGATGCGGCGCTCGGCACGCTGTACGGCTACCGC
GCGCACGGCCCCTACGATCCCGCGCACGGCCACCGCTTCAACCCGCACAAGCTGCTGCTCGACCCGTATGCGCGCCAGTT
GAGCGGCCCGGTGCGCTGGTCCGATGCGCTGTTCGGCTACCGCCTGCAAAGCGCGCGCGGCGACCTGACACCGGACCGCC
GCGACAGCGCCCCCACCATGCCCAAGGCGGTGGTGGTCGACGAGAGCTTCCACTGGGGCGACGACCGCCCGCCCGCGGTG
CCGTGGCCGTCCACCGTCATCTATGAAGCCCACCTGCGGGGCCTGTCGATGCTGCGCGGCGATCTGCTGCCCAACCTGCG
CGGCTCCTTCGCCGCGCTGTGCGACCCGCGCTTTATCGACCACCTGGTGCAGCTCGGCATCACCACCGTCGAGCTGCTGC
CGGTCCACGCCATCCTGCAGGACCGCTTCCTGCTGGAGCGCGGACTGCGCAACTACTGGGGCTACAACACCATGGCGTAC
TTCGCGCCGGAGCCCGCTTACCTGGGCACCGGGCAACTGCAGGAGTTCAAGGTGGCGATGCGGCGCCTGCACGCGGCCGG
CATCGAAGTGATCCTGGACGTGGTCTACAACCATACCTGCGAGGGCAACGAGCTGGGGCCAACGGTGTCGTGGCGCGGGC
TGGACAACGCCAGCTACTACCGGCTGGTGCCCGGCGACGAGCGCCACTACATCAACGACACCGGCTGCGGCAATACGCTC
AACCTGTCGCATCCGCGCGTGCTGCAGATGGTGATGGACTCGTTGCGCTACTGGGTGCACTGCTACCACGTCGATGGCTT
CCGCTTCGACCTGGGCACCACCCTCGGGCGCGAGGGCAACGGCTTCGATGCGGGCTCGGGCTTTTTCGATGCACTGATGC
AGGATCCGGTGCTGAGCCGCGTCAAGCTGATCTCCGAGCCGTGGGACCTGGGCCCGGGCGGCTACCAGCTTGGCAACCAC
CCGCCCGGCTTTGCCGAGTGGAACGACAAGTTCCGCGACGTGGCGCGCCGCTTCTGGCGCGGCGACGCCGGCCACCGCGG
CGAACTGGCGGCGCGGCTGGCGGCCTCGGCCGACCTCTTCGACCGCCGCCACCGGCGCCCGTGGGCCAGCATCAACTTCA
TCACCGCACACGACGGCTTCACGCTGGCCGACCTGGTCAGCTACAGCAGCAAGCACAACGAGGCCAACGGCGAAGACAAC
CGCGACGGCACCGACGACAACGCCAGCGCCAACTGGAGCCCCGACGGCAGCATCGAAGGCCCCACCGACGACCCCGCTAT
CCTGGCCTGCCGCGCGCGCGTGGCACAGGCACTGATGGCCACGCTGCTGCTGTCGCAGGGCACGCCGATGCTGACCGCCG
GCGATGAATGGGGCCGCAGCCAGCAAGGCAACAACAACGCCTACTGCCAGGACGACGAGATCTCGTGGCTGGACTGGAAG
CAGGCCCAGACGCCCGGCGCCCAGCCGCTGCAGCACATGGTGCGCCGCCTGCTGGACCTGCGCCGGCGGCTGCACTCCCT
GGCCGGCGACCGCTTCGCGCACGGGCGCGAGGAACCGGCGCAGGGCATTGCCGATATTGCCTGGTTCGATACCGACGGCA
AACCGCCCACCCCCGAGGAATGGGCCGACCCGGAGATCCGCACGCTCGCGCTGCGCCGCGCGGCCGCGCCGCCCCAACCG
GAGCGCGCGCAGCTGGGCTCGCTGGCGCAGGACGAAGAGAACGATGCCGGCGCCGTGCAGGTAACCTTGCTGCTGCTCAA
CGCCGGCGATGCCGAGGCCTCCTTCCTGCTGCCCGGGCCCACGCTGGCCTGGAAACTGCTGTTCGACAGCAGCCGGCCCG
ACGCCACCGAGGCCGAACTCGCGAGCGAGGCGGTCGCCGACAGCCAGGCGGTGGCGGGCCACTCGCTGGTGCTGCTGGCG
GCCCAGGCCCGCAGCCGCCAGAGCGCGCCCCAGGAAAGCCCGCCGCTGGGCGCGCCGCAGGAGGCACAGGCATGA

Upstream 100 bases:

>100_bases
CTTCGCACGGGCAGCCGGCTTCGCTCGCGCTGACGCTGCCGCCGCTGGCCACGCTGGTGCTGCGCTTCGATCCTGGTGGC
GGCATCCAGGGCGCATCGAC

Downstream 100 bases:

>100_bases
GCCGGCGCGCCACCCGCCACGACACGCCCACGGCGCTGCATGCGCTGGCACTGCGCGCGGGCCTGCTGCCCCGGTGGACG
GATGCCTGGAACCAGCCCCA

Product: Type II secretory pathway, pullulanase PulA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MSRQFADRLLPGKPYPLGAHWDGLGVNFAVFSAHASRIDLCLFSDNGRKELARLQLPERTDEIWHGYLPDAALGTLYGYR
AHGPYDPAHGHRFNPHKLLLDPYARQLSGPVRWSDALFGYRLQSARGDLTPDRRDSAPTMPKAVVVDESFHWGDDRPPAV
PWPSTVIYEAHLRGLSMLRGDLLPNLRGSFAALCDPRFIDHLVQLGITTVELLPVHAILQDRFLLERGLRNYWGYNTMAY
FAPEPAYLGTGQLQEFKVAMRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYYRLVPGDERHYINDTGCGNTL
NLSHPRVLQMVMDSLRYWVHCYHVDGFRFDLGTTLGREGNGFDAGSGFFDALMQDPVLSRVKLISEPWDLGPGGYQLGNH
PPGFAEWNDKFRDVARRFWRGDAGHRGELAARLAASADLFDRRHRRPWASINFITAHDGFTLADLVSYSSKHNEANGEDN
RDGTDDNASANWSPDGSIEGPTDDPAILACRARVAQALMATLLLSQGTPMLTAGDEWGRSQQGNNNAYCQDDEISWLDWK
QAQTPGAQPLQHMVRRLLDLRRRLHSLAGDRFAHGREEPAQGIADIAWFDTDGKPPTPEEWADPEIRTLALRRAAAPPQP
ERAQLGSLAQDEENDAGAVQVTLLLLNAGDAEASFLLPGPTLAWKLLFDSSRPDATEAELASEAVADSQAVAGHSLVLLA
AQARSRQSAPQESPPLGAPQEAQA

Sequences:

>Translated_744_residues
MSRQFADRLLPGKPYPLGAHWDGLGVNFAVFSAHASRIDLCLFSDNGRKELARLQLPERTDEIWHGYLPDAALGTLYGYR
AHGPYDPAHGHRFNPHKLLLDPYARQLSGPVRWSDALFGYRLQSARGDLTPDRRDSAPTMPKAVVVDESFHWGDDRPPAV
PWPSTVIYEAHLRGLSMLRGDLLPNLRGSFAALCDPRFIDHLVQLGITTVELLPVHAILQDRFLLERGLRNYWGYNTMAY
FAPEPAYLGTGQLQEFKVAMRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYYRLVPGDERHYINDTGCGNTL
NLSHPRVLQMVMDSLRYWVHCYHVDGFRFDLGTTLGREGNGFDAGSGFFDALMQDPVLSRVKLISEPWDLGPGGYQLGNH
PPGFAEWNDKFRDVARRFWRGDAGHRGELAARLAASADLFDRRHRRPWASINFITAHDGFTLADLVSYSSKHNEANGEDN
RDGTDDNASANWSPDGSIEGPTDDPAILACRARVAQALMATLLLSQGTPMLTAGDEWGRSQQGNNNAYCQDDEISWLDWK
QAQTPGAQPLQHMVRRLLDLRRRLHSLAGDRFAHGREEPAQGIADIAWFDTDGKPPTPEEWADPEIRTLALRRAAAPPQP
ERAQLGSLAQDEENDAGAVQVTLLLLNAGDAEASFLLPGPTLAWKLLFDSSRPDATEAELASEAVADSQAVAGHSLVLLA
AQARSRQSAPQESPPLGAPQEAQA
>Mature_743_residues
SRQFADRLLPGKPYPLGAHWDGLGVNFAVFSAHASRIDLCLFSDNGRKELARLQLPERTDEIWHGYLPDAALGTLYGYRA
HGPYDPAHGHRFNPHKLLLDPYARQLSGPVRWSDALFGYRLQSARGDLTPDRRDSAPTMPKAVVVDESFHWGDDRPPAVP
WPSTVIYEAHLRGLSMLRGDLLPNLRGSFAALCDPRFIDHLVQLGITTVELLPVHAILQDRFLLERGLRNYWGYNTMAYF
APEPAYLGTGQLQEFKVAMRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYYRLVPGDERHYINDTGCGNTLN
LSHPRVLQMVMDSLRYWVHCYHVDGFRFDLGTTLGREGNGFDAGSGFFDALMQDPVLSRVKLISEPWDLGPGGYQLGNHP
PGFAEWNDKFRDVARRFWRGDAGHRGELAARLAASADLFDRRHRRPWASINFITAHDGFTLADLVSYSSKHNEANGEDNR
DGTDDNASANWSPDGSIEGPTDDPAILACRARVAQALMATLLLSQGTPMLTAGDEWGRSQQGNNNAYCQDDEISWLDWKQ
AQTPGAQPLQHMVRRLLDLRRRLHSLAGDRFAHGREEPAQGIADIAWFDTDGKPPTPEEWADPEIRTLALRRAAAPPQPE
RAQLGSLAQDEENDAGAVQVTLLLLNAGDAEASFLLPGPTLAWKLLFDSSRPDATEAELASEAVADSQAVAGHSLVLLAA
QARSRQSAPQESPPLGAPQEAQA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=618, Percent_Identity=50.4854368932039, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82374; Mature: 82243

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQFADRLLPGKPYPLGAHWDGLGVNFAVFSAHASRIDLCLFSDNGRKELARLQLPERT
CCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHCCCCHH
DEIWHGYLPDAALGTLYGYRAHGPYDPAHGHRFNPHKLLLDPYARQLSGPVRWSDALFGY
HHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHCCCCCHHHHHHHH
RLQSARGDLTPDRRDSAPTMPKAVVVDESFHWGDDRPPAVPWPSTVIYEAHLRGLSMLRG
HHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHH
DLLPNLRGSFAALCDPRFIDHLVQLGITTVELLPVHAILQDRFLLERGLRNYWGYNTMAY
HHCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FAPEPAYLGTGQLQEFKVAMRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYY
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEE
RLVPGDERHYINDTGCGNTLNLSHPRVLQMVMDSLRYWVHCYHVDGFRFDLGTTLGREGN
EECCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCEEEECCCCCCCCCC
GFDAGSGFFDALMQDPVLSRVKLISEPWDLGPGGYQLGNHPPGFAEWNDKFRDVARRFWR
CCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GDAGHRGELAARLAASADLFDRRHRRPWASINFITAHDGFTLADLVSYSSKHNEANGEDN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCC
RDGTDDNASANWSPDGSIEGPTDDPAILACRARVAQALMATLLLSQGTPMLTAGDEWGRS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCC
QQGNNNAYCQDDEISWLDWKQAQTPGAQPLQHMVRRLLDLRRRLHSLAGDRFAHGREEPA
CCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHH
QGIADIAWFDTDGKPPTPEEWADPEIRTLALRRAAAPPQPERAQLGSLAQDEENDAGAVQ
CCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEE
VTLLLLNAGDAEASFLLPGPTLAWKLLFDSSRPDATEAELASEAVADSQAVAGHSLVLLA
EEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
AQARSRQSAPQESPPLGAPQEAQA
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRQFADRLLPGKPYPLGAHWDGLGVNFAVFSAHASRIDLCLFSDNGRKELARLQLPERT
CHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHCCCCHH
DEIWHGYLPDAALGTLYGYRAHGPYDPAHGHRFNPHKLLLDPYARQLSGPVRWSDALFGY
HHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHCCCCCHHHHHHHH
RLQSARGDLTPDRRDSAPTMPKAVVVDESFHWGDDRPPAVPWPSTVIYEAHLRGLSMLRG
HHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHH
DLLPNLRGSFAALCDPRFIDHLVQLGITTVELLPVHAILQDRFLLERGLRNYWGYNTMAY
HHCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FAPEPAYLGTGQLQEFKVAMRRLHAAGIEVILDVVYNHTCEGNELGPTVSWRGLDNASYY
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEE
RLVPGDERHYINDTGCGNTLNLSHPRVLQMVMDSLRYWVHCYHVDGFRFDLGTTLGREGN
EECCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCEEEECCCCCCCCCC
GFDAGSGFFDALMQDPVLSRVKLISEPWDLGPGGYQLGNHPPGFAEWNDKFRDVARRFWR
CCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GDAGHRGELAARLAASADLFDRRHRRPWASINFITAHDGFTLADLVSYSSKHNEANGEDN
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCC
RDGTDDNASANWSPDGSIEGPTDDPAILACRARVAQALMATLLLSQGTPMLTAGDEWGRS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCC
QQGNNNAYCQDDEISWLDWKQAQTPGAQPLQHMVRRLLDLRRRLHSLAGDRFAHGREEPA
CCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHH
QGIADIAWFDTDGKPPTPEEWADPEIRTLALRRAAAPPQPERAQLGSLAQDEENDAGAVQ
CCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEE
VTLLLLNAGDAEASFLLPGPTLAWKLLFDSSRPDATEAELASEAVADSQAVAGHSLVLLA
EEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
AQARSRQSAPQESPPLGAPQEAQA
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]