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The map label for this gene is glgB

Identifier: 116695500

GI number: 116695500

Start: 1751921

End: 1754191

Strand: Direct

Name: glgB

Synonym: H16_B1559

Alternate gene names: 116695500

Gene position: 1751921-1754191 (Clockwise)

Preceding gene: 116695499

Following gene: 116695501

Centisome position: 60.15

GC content: 70.32

Gene sequence:

>2271_bases
ATGACCCCCGCCCATGGCACGCAGCCCGGCATGCTGCCGGGCCACGAGTTCGAGGCCTTGCTTGGTGCGCGCCACCATGA
TCCGTTTGCCGTGCTGGGCCCGCACCCTGACGGCGACGGCACGCTGGTGCGCGCCTGCCTGCCAGGGGCGGCGTCGGTGC
AGCTGACCGATGCGGGCGGCACGCCACTGGCGGAGATGGCACGGCTGCATGGCGGCGGCGTCTTCGCCGCGCGGCTGCCG
CGCGAATACAAGGGCGGTGCGCCCGATTACCGGCTGCGCGTGCAATGGTCCGACGGCAGCGGGCAGTGCGGCGCCGACCC
TTACGCCTTTGGCCTGCTGCTGGGTGAGCTGGACCTGCACCTGATCGCCGAGGGCCGCCACTTCGAGCTCGGCGCCTGCC
TGGGCGCGCAGTGGCAGCGCGTGGACGGCATCGACGGCGTGCGCTTTGCCGTATGGGCGCCGAACGCGCGGCGGGTCTCT
GTGATCGCTGACTTCAACGGCTGGCATCCGGCGCGGCACCCGATGCGGCTGCGCCACCCCAGCGGCATCTGGGAGCTGTT
CATCCCCGCCGCACTGGGCGCGCAGCCCGGCAGCCGCTACAAGTACGACCTGCTCGACCCGCACGGCACCGAGCTGCCGG
ACAAGGCCGACCCGCTCGCGCTGGCCACCGAGGCGCCCCCCGCCACCGCCTCGGTGGTGGCCGGACCGGGCCAGGGCGCG
CCCCCCTTCGCCTGGCATGACGCCGACTGGATGGCGCGCCGCGGCGGCGCCGATCCCTACGCAGCGCCGATGTCGGTCTA
CGAGGTCCACGCTCTGTCGTGGCTGCGCGCGGCCAACGACACGCAGCGCGGCTGGGAGATCCTGGCCGAGCGGCTGGTGC
CATACGTGCAGGAACTGGGCTTTACGCATATCGAACTGCTGCCGATCACCGAGCATCCGTTCGGCGGCTCGTGGGGCTAC
CAGCCGCTGTCGCTGTATGCGCCCACCGCGCGGCTGGGGCCGCCGCAGGCCTTCGCCGCCTTTATCGACCGCTGCCACCA
GGCCGGCATCGGCGTGCTCCTGGACTGGGTGCCCGCGCACTTCCCGACCGATCCGCACGGGCTGGCGCGCTTCGACGGCA
CCGCCCTGTATGAGCACGAGGACCCGCGCGAAGGCTTCCACCAGGACTGGAACACCCTGATCTACAACCTCGGCCGCAAC
GAGGTGCGCGGCTTCCTGCTGGCCGGCGCGCTGCACTGGCTGGAGCACTTCCACGCCGACGGCCTGCGCGTGGACGCGGT
GGCATCCATGCTCTACCGCGACTACAGCCGCGAGCCCGGCCAGTGGGTGCCGAACCGCTTCGGCGGCCGGGAAAACCTGG
AAGCCATCGACTTCCTGCGCGAACTGAACGCCGTGGTCCACGAACGCTGCCCCGGTGCGCTGACCATCGCCGAAGAATCC
ACCGCCTGGCCCGGCGTGACCGCCAGCGTCGCCAGCGGCGGGCTTGGCTTCGACTTCAAGTGGAACATGGGCTGGATGCA
CGACACGCTGCATTATCTCGGTCACGAGCCCGTCCATCGCGCCTGGCACCACCAGGACATGACCTTCGGGCTGGTCTATG
CGTGGTCCGAGGCCTTCGTGCTGCCGCTGTCGCATGACGAGGTGGTGCACGGCAAGGCCTCGATGATCGGCAAGGTGCCC
GGCGACGAGTGGCAGCGCTTTGCCGGCCTGCGCGCCTACTACGGCTTCATGTGGGCGCACCCGGGCAAGAAGCTGCTGTT
CATGGGCGGCGAACTGGCGCAGTGGCAGGAATGGAACCACGATGCCGAGCTTGACTGGGCCTTGCTGGACCACCCCATGC
ACCGCGGCATGCACACGCTGGTGCGGGATCTGAACCGCCTCTACCGGGAGCTGCCCGCGCTGCATGCGCTGGACCACCGC
CCGGAAGGCTTCCAGTGGGTGGTGGGCGACGACAACCATAACAGCGTGTTTGCGTGGCTGCGCCGCGCGGGCCCATACAG
CCGCGAAGTGGTGCTGGTGGTGGTCAACATGACCCCGGTGCCGCGCTATGGCTACCGGCTCGGGGTGCCGTATGCCGGCG
CCTGGCAGGAATGCCTGAATACCGATGCCGCCGTGTATGGCGGCACCAATGTCGGCAACAGCGGCGCGGTAGCGGCAGTG
GACGTGCCTTCGCACGGGCAGCCGGCTTCGCTCGCGCTGACGCTGCCGCCGCTGGCCACGCTGGTGCTGCGCTTCGATCC
TGGTGGCGGCATCCAGGGCGCATCGACATGA

Upstream 100 bases:

>100_bases
AGGTCGACTACGAAGCCGCCAACCGCGTGGCTTGGATCGACCTGCCGGTCAGCGGCCTGGCGCGCCTGGTGCGCAAGCTG
CTGGCGCCGGGAGACCAGCC

Downstream 100 bases:

>100_bases
GCCGACAGTTCGCGGACCGGCTGCTGCCGGGCAAGCCGTATCCCCTCGGGGCCCACTGGGACGGCCTGGGTGTCAATTTC
GCGGTGTTCTCGGCCCATGC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTPAHGTQPGMLPGHEFEALLGARHHDPFAVLGPHPDGDGTLVRACLPGAASVQLTDAGGTPLAEMARLHGGGVFAARLP
REYKGGAPDYRLRVQWSDGSGQCGADPYAFGLLLGELDLHLIAEGRHFELGACLGAQWQRVDGIDGVRFAVWAPNARRVS
VIADFNGWHPARHPMRLRHPSGIWELFIPAALGAQPGSRYKYDLLDPHGTELPDKADPLALATEAPPATASVVAGPGQGA
PPFAWHDADWMARRGGADPYAAPMSVYEVHALSWLRAANDTQRGWEILAERLVPYVQELGFTHIELLPITEHPFGGSWGY
QPLSLYAPTARLGPPQAFAAFIDRCHQAGIGVLLDWVPAHFPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRN
EVRGFLLAGALHWLEHFHADGLRVDAVASMLYRDYSREPGQWVPNRFGGRENLEAIDFLRELNAVVHERCPGALTIAEES
TAWPGVTASVASGGLGFDFKWNMGWMHDTLHYLGHEPVHRAWHHQDMTFGLVYAWSEAFVLPLSHDEVVHGKASMIGKVP
GDEWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNHDAELDWALLDHPMHRGMHTLVRDLNRLYRELPALHALDHR
PEGFQWVVGDDNHNSVFAWLRRAGPYSREVVLVVVNMTPVPRYGYRLGVPYAGAWQECLNTDAAVYGGTNVGNSGAVAAV
DVPSHGQPASLALTLPPLATLVLRFDPGGGIQGAST

Sequences:

>Translated_756_residues
MTPAHGTQPGMLPGHEFEALLGARHHDPFAVLGPHPDGDGTLVRACLPGAASVQLTDAGGTPLAEMARLHGGGVFAARLP
REYKGGAPDYRLRVQWSDGSGQCGADPYAFGLLLGELDLHLIAEGRHFELGACLGAQWQRVDGIDGVRFAVWAPNARRVS
VIADFNGWHPARHPMRLRHPSGIWELFIPAALGAQPGSRYKYDLLDPHGTELPDKADPLALATEAPPATASVVAGPGQGA
PPFAWHDADWMARRGGADPYAAPMSVYEVHALSWLRAANDTQRGWEILAERLVPYVQELGFTHIELLPITEHPFGGSWGY
QPLSLYAPTARLGPPQAFAAFIDRCHQAGIGVLLDWVPAHFPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRN
EVRGFLLAGALHWLEHFHADGLRVDAVASMLYRDYSREPGQWVPNRFGGRENLEAIDFLRELNAVVHERCPGALTIAEES
TAWPGVTASVASGGLGFDFKWNMGWMHDTLHYLGHEPVHRAWHHQDMTFGLVYAWSEAFVLPLSHDEVVHGKASMIGKVP
GDEWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNHDAELDWALLDHPMHRGMHTLVRDLNRLYRELPALHALDHR
PEGFQWVVGDDNHNSVFAWLRRAGPYSREVVLVVVNMTPVPRYGYRLGVPYAGAWQECLNTDAAVYGGTNVGNSGAVAAV
DVPSHGQPASLALTLPPLATLVLRFDPGGGIQGAST
>Mature_755_residues
TPAHGTQPGMLPGHEFEALLGARHHDPFAVLGPHPDGDGTLVRACLPGAASVQLTDAGGTPLAEMARLHGGGVFAARLPR
EYKGGAPDYRLRVQWSDGSGQCGADPYAFGLLLGELDLHLIAEGRHFELGACLGAQWQRVDGIDGVRFAVWAPNARRVSV
IADFNGWHPARHPMRLRHPSGIWELFIPAALGAQPGSRYKYDLLDPHGTELPDKADPLALATEAPPATASVVAGPGQGAP
PFAWHDADWMARRGGADPYAAPMSVYEVHALSWLRAANDTQRGWEILAERLVPYVQELGFTHIELLPITEHPFGGSWGYQ
PLSLYAPTARLGPPQAFAAFIDRCHQAGIGVLLDWVPAHFPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRNE
VRGFLLAGALHWLEHFHADGLRVDAVASMLYRDYSREPGQWVPNRFGGRENLEAIDFLRELNAVVHERCPGALTIAEEST
AWPGVTASVASGGLGFDFKWNMGWMHDTLHYLGHEPVHRAWHHQDMTFGLVYAWSEAFVLPLSHDEVVHGKASMIGKVPG
DEWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNHDAELDWALLDHPMHRGMHTLVRDLNRLYRELPALHALDHRP
EGFQWVVGDDNHNSVFAWLRRAGPYSREVVLVVVNMTPVPRYGYRLGVPYAGAWQECLNTDAAVYGGTNVGNSGAVAAVD
VPSHGQPASLALTLPPLATLVLRFDPGGGIQGAST

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=638, Percent_Identity=26.6457680250784, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1789839, Length=731, Percent_Identity=52.9411764705882, Blast_Score=744, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=637, Percent_Identity=24.8037676609105, Blast_Score=157, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI32564391, Length=564, Percent_Identity=25, Blast_Score=141, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320826, Length=652, Percent_Identity=25, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI28573410, Length=601, Percent_Identity=27.6206322795341, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_CUPNH (Q0K0X8)

Other databases:

- EMBL:   AM260480
- RefSeq:   YP_841076.1
- ProteinModelPortal:   Q0K0X8
- STRING:   Q0K0X8
- GeneID:   4455403
- GenomeReviews:   AM260480_GR
- KEGG:   reh:H16_B1559
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   Q0K0X8
- ProtClustDB:   PRK05402
- BioCyc:   REUT381666:H16_B1559-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 83099; Mature: 82967

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: ACT_SITE 320-320 ACT_SITE 355-355 ACT_SITE 360-360 ACT_SITE 423-423 ACT_SITE 425-425 ACT_SITE 478-478 ACT_SITE 545-545 ACT_SITE 546-546

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAHGTQPGMLPGHEFEALLGARHHDPFAVLGPHPDGDGTLVRACLPGAASVQLTDAGG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEECCCC
TPLAEMARLHGGGVFAARLPREYKGGAPDYRLRVQWSDGSGQCGADPYAFGLLLGELDLH
CCHHHHHHHCCCCEEEECCCHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCEE
LIAEGRHFELGACLGAQWQRVDGIDGVRFAVWAPNARRVSVIADFNGWHPARHPMRLRHP
EEECCCEEEEEHHHCCCHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCEEECCC
SGIWELFIPAALGAQPGSRYKYDLLDPHGTELPDKADPLALATEAPPATASVVAGPGQGA
CCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCC
PPFAWHDADWMARRGGADPYAAPMSVYEVHALSWLRAANDTQRGWEILAERLVPYVQELG
CCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
FTHIELLPITEHPFGGSWGYQPLSLYAPTARLGPPQAFAAFIDRCHQAGIGVLLDWVPAH
CCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHCCC
FPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRNEVRGFLLAGALHWLEHFHAD
CCCCCCCHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
GLRVDAVASMLYRDYSREPGQWVPNRFGGRENLEAIDFLRELNAVVHERCPGALTIAEES
CEEHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
TAWPGVTASVASGGLGFDFKWNMGWMHDTLHYLGHEPVHRAWHHQDMTFGLVYAWSEAFV
CCCCCCCHHHHCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEECCEEE
LPLSHDEVVHGKASMIGKVPGDEWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNH
EECCCCCEECCHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCHHHHHHCCC
DAELDWALLDHPMHRGMHTLVRDLNRLYRELPALHALDHRPEGFQWVVGDDNHNSVFAWL
CCCEEHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEECCCCCCHHHHHH
RRAGPYSREVVLVVVNMTPVPRYGYRLGVPYAGAWQECLNTDAAVYGGTNVGNSGAVAAV
HHCCCCCCEEEEEEEECCCCCCCCEEECCCCCHHHHHHCCCCCEEECCCCCCCCCCEEEE
DVPSHGQPASLALTLPPLATLVLRFDPGGGIQGAST
ECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TPAHGTQPGMLPGHEFEALLGARHHDPFAVLGPHPDGDGTLVRACLPGAASVQLTDAGG
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEECCCC
TPLAEMARLHGGGVFAARLPREYKGGAPDYRLRVQWSDGSGQCGADPYAFGLLLGELDLH
CCHHHHHHHCCCCEEEECCCHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCEE
LIAEGRHFELGACLGAQWQRVDGIDGVRFAVWAPNARRVSVIADFNGWHPARHPMRLRHP
EEECCCEEEEEHHHCCCHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCEEECCC
SGIWELFIPAALGAQPGSRYKYDLLDPHGTELPDKADPLALATEAPPATASVVAGPGQGA
CCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCC
PPFAWHDADWMARRGGADPYAAPMSVYEVHALSWLRAANDTQRGWEILAERLVPYVQELG
CCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
FTHIELLPITEHPFGGSWGYQPLSLYAPTARLGPPQAFAAFIDRCHQAGIGVLLDWVPAH
CCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHCCC
FPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRNEVRGFLLAGALHWLEHFHAD
CCCCCCCHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
GLRVDAVASMLYRDYSREPGQWVPNRFGGRENLEAIDFLRELNAVVHERCPGALTIAEES
CEEHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
TAWPGVTASVASGGLGFDFKWNMGWMHDTLHYLGHEPVHRAWHHQDMTFGLVYAWSEAFV
CCCCCCCHHHHCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCEEEEEEEECCEEE
LPLSHDEVVHGKASMIGKVPGDEWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNH
EECCCCCEECCHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCHHHHHHCCC
DAELDWALLDHPMHRGMHTLVRDLNRLYRELPALHALDHRPEGFQWVVGDDNHNSVFAWL
CCCEEHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEECCCCCCHHHHHH
RRAGPYSREVVLVVVNMTPVPRYGYRLGVPYAGAWQECLNTDAAVYGGTNVGNSGAVAAV
HHCCCCCCEEEEEEEECCCCCCCCEEECCCCCHHHHHHCCCCCEEECCCCCCCCCCEEEE
DVPSHGQPASLALTLPPLATLVLRFDPGGGIQGAST
ECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA