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The map label for this gene is fdoH [H]

Identifier: 116695395

GI number: 116695395

Start: 1629311

End: 1630201

Strand: Direct

Name: fdoH [H]

Synonym: H16_B1453

Alternate gene names: 116695395

Gene position: 1629311-1630201 (Clockwise)

Preceding gene: 116695394

Following gene: 116695396

Centisome position: 55.94

GC content: 64.98

Gene sequence:

>891_bases
ATGAACTCGCAGAACATCATCCGTAGCTCGGCCTCGTCGGAGCTGACACCAGCCCCTCGTATCCGCGACCATCAGATGGA
AGTGGCCAAGCTGATCGACGTGTCGATCTGCATCGGCTGCAAGGCGTGCCAGGTCGCCTGCAACGAATGGAACGACCTGC
GCGGCGACGTGCAGGAGAATGTCGGCGTCTACGACAATCCGCGCGACCTGTCGCCCAACACCTGGACCCTGATGCGCTTC
ACCGAGCATGAGGACCAGGCGGGCAAGCTGGAATGGCTGATCCGCAAGGATGGCTGCATGCATTGCGAGGATCCCGGCTG
CCTGAAAGCCTGCCCGGCGCCGGGCGCCATCGTCCAGTACGCCAACGGCATCGTCGACTTCCAGTCCGACCTGTGCATCG
GCTGCGGCTATTGCGTCGCGGGCTGCCCCTTCGACGTGCCGCGTATTTCCAAGCTGGACAACAAGGCCTACAAGTGCACG
CTGTGCTCGGACCGCGTCTCGGTCGGGCAGGAGCCTGCCTGCGTCAAGACCTGCCCGACCGGCGCCATCGCCTTTGGCTC
GAAGGCAGAGATGCAATCCCTTGCCGGCGAGCGCGTGGCCGAGTTGAATGAGCGCGGCTATGCCAGGGCCGGCCTGTATG
ACCCGGCGGGGGTCGGCGGCACCCACGTGATGTATGTGCTGCAACATGCCGACGACCCGAAGCGTTACGCGGGCTTGCCG
AAGGACCCGGAGATCAGCTCGGTGGTCTCCGGCTGGAAGGATGGCCTGAAACCCGTAGCCGGCTTCGTCGGCGCGGCGGC
GGTCGTGGGCATGGCGGCGCATTTCATGGCGGTCGGCCCCAACATCACCGAATCAGACGAACACGACGCCCCGACTGACG
GCGCAGCATAA

Upstream 100 bases:

>100_bases
TCGCCAACACGCTTGCGCCCGGTGTCGGCGACGCCAATTCGCAAACGCCGGAATTCAAGGCATTCCTTGTAAACATTGAA
AAAGTGGCGGGAGCGCAAGC

Downstream 100 bases:

>100_bases
GCGGAGACCGGAAATGGCGACCAACAACGACAGGATCCTGCGCACGAAATTCGCCGAGCGGCTGTGTCACTGGGCGATCG
TGCTCTGCTTCTTCCTTGCG

Product: formate dehydrogenase beta subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNSQNIIRSSASSELTPAPRIRDHQMEVAKLIDVSICIGCKACQVACNEWNDLRGDVQENVGVYDNPRDLSPNTWTLMRF
TEHEDQAGKLEWLIRKDGCMHCEDPGCLKACPAPGAIVQYANGIVDFQSDLCIGCGYCVAGCPFDVPRISKLDNKAYKCT
LCSDRVSVGQEPACVKTCPTGAIAFGSKAEMQSLAGERVAELNERGYARAGLYDPAGVGGTHVMYVLQHADDPKRYAGLP
KDPEISSVVSGWKDGLKPVAGFVGAAAVVGMAAHFMAVGPNITESDEHDAPTDGAA

Sequences:

>Translated_296_residues
MNSQNIIRSSASSELTPAPRIRDHQMEVAKLIDVSICIGCKACQVACNEWNDLRGDVQENVGVYDNPRDLSPNTWTLMRF
TEHEDQAGKLEWLIRKDGCMHCEDPGCLKACPAPGAIVQYANGIVDFQSDLCIGCGYCVAGCPFDVPRISKLDNKAYKCT
LCSDRVSVGQEPACVKTCPTGAIAFGSKAEMQSLAGERVAELNERGYARAGLYDPAGVGGTHVMYVLQHADDPKRYAGLP
KDPEISSVVSGWKDGLKPVAGFVGAAAVVGMAAHFMAVGPNITESDEHDAPTDGAA
>Mature_296_residues
MNSQNIIRSSASSELTPAPRIRDHQMEVAKLIDVSICIGCKACQVACNEWNDLRGDVQENVGVYDNPRDLSPNTWTLMRF
TEHEDQAGKLEWLIRKDGCMHCEDPGCLKACPAPGAIVQYANGIVDFQSDLCIGCGYCVAGCPFDVPRISKLDNKAYKCT
LCSDRVSVGQEPACVKTCPTGAIAFGSKAEMQSLAGERVAELNERGYARAGLYDPAGVGGTHVMYVLQHADDPKRYAGLP
KDPEISSVVSGWKDGLKPVAGFVGAAAVVGMAAHFMAVGPNITESDEHDAPTDGAA

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=287, Percent_Identity=68.6411149825784, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1787749, Length=290, Percent_Identity=66.8965517241379, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1789370, Length=221, Percent_Identity=30.316742081448, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787122, Length=170, Percent_Identity=33.5294117647059, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1787872, Length=170, Percent_Identity=32.3529411764706, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI2367345, Length=156, Percent_Identity=35.8974358974359, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI226510944, Length=216, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788811, Length=170, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082179, Length=178, Percent_Identity=28.6516853932584, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=30.5389221556886, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=112, Percent_Identity=29.4642857142857, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787478, Length=112, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787963, Length=147, Percent_Identity=25.1700680272109, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 31640; Mature: 31640

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQNIIRSSASSELTPAPRIRDHQMEVAKLIDVSICIGCKACQVACNEWNDLRGDVQEN
CCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHC
VGVYDNPRDLSPNTWTLMRFTEHEDQAGKLEWLIRKDGCMHCEDPGCLKACPAPGAIVQY
CCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
ANGIVDFQSDLCIGCGYCVAGCPFDVPRISKLDNKAYKCTLCSDRVSVGQEPACVKTCPT
HCCCEECCCCHHEECCHHHCCCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCEEEECCC
GAIAFGSKAEMQSLAGERVAELNERGYARAGLYDPAGVGGTHVMYVLQHADDPKRYAGLP
CCEECCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEECCCCHHHHCCCC
KDPEISSVVSGWKDGLKPVAGFVGAAAVVGMAAHFMAVGPNITESDEHDAPTDGAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSQNIIRSSASSELTPAPRIRDHQMEVAKLIDVSICIGCKACQVACNEWNDLRGDVQEN
CCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHC
VGVYDNPRDLSPNTWTLMRFTEHEDQAGKLEWLIRKDGCMHCEDPGCLKACPAPGAIVQY
CCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
ANGIVDFQSDLCIGCGYCVAGCPFDVPRISKLDNKAYKCTLCSDRVSVGQEPACVKTCPT
HCCCEECCCCHHEECCHHHCCCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCEEEECCC
GAIAFGSKAEMQSLAGERVAELNERGYARAGLYDPAGVGGTHVMYVLQHADDPKRYAGLP
CCEECCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEECCCCHHHHCCCC
KDPEISSVVSGWKDGLKPVAGFVGAAAVVGMAAHFMAVGPNITESDEHDAPTDGAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]