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The map label for this gene is fdoI [H]

Identifier: 116695396

GI number: 116695396

Start: 1630215

End: 1630880

Strand: Direct

Name: fdoI [H]

Synonym: H16_B1454

Alternate gene names: 116695396

Gene position: 1630215-1630880 (Clockwise)

Preceding gene: 116695395

Following gene: 116695397

Centisome position: 55.97

GC content: 63.66

Gene sequence:

>666_bases
ATGGCGACCAACAACGACAGGATCCTGCGCACGAAATTCGCCGAGCGGCTGTGTCACTGGGCGATCGTGCTCTGCTTCTT
CCTTGCGGCGGTTTCGGGAATCTCGTGGTTCTTCCCGACGGTGAGCTGGCTGAGCGGCTTCCTTGGCACGCCGCAGATGG
CACGGCTGCTCCACCCGTTCCTGGGCATCGCGGTGTTCGCGGGGCTGTGCTACATGTTCTGGCGCTTTGTCGGCCACAAC
ATGCCCGCGCGCACCGACGCGATCTGGTTCCGCCGCGCCAGGGAAGTGCTGCTGAACCAGCATGGTGGCGAACCGCTGCA
GATCGGCAAGTACAACGCCGGCCAGAAGGTGCTGTTCTGGCTCATTATGGCTTCGATCCTGGCCCTGCTGGTCTCAGGCC
TGATCATGTGGCGCGCCTATTTCGCCGAGTATTTCCCGATTCCCGTGCTCCGCCTGGCGATCCTGGCCCATTCCGTCGCG
GGGATCGGCCTGATCCTGCTGATCGTGGGCCACATCTATCTGGCGATCTGGGTCCGCGGCTCGATCACCGGCATGGTGAC
GGGCTACGTCTCGCGCGCCTGGGCCAGGCAGCACCATGACCGCTGGTATGGCGAGTTGCAGGCAAAGGAGTCGAAAGCGG
CGCAAGCGAAGGGGAACGAGGCATGA

Upstream 100 bases:

>100_bases
GTGGGCATGGCGGCGCATTTCATGGCGGTCGGCCCCAACATCACCGAATCAGACGAACACGACGCCCCGACTGACGGCGC
AGCATAAGCGGAGACCGGAA

Downstream 100 bases:

>100_bases
ACCAGCATCAGGCCACGCCGGGAACACTGCCATCGGACGAGGCCTCCCGGCATTTCACGCCGCTGATCCAGCCAGACCTT
GCCGGTCTTTACAGCCGCCG

Product: formate dehydrogenase gamma subunit

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MATNNDRILRTKFAERLCHWAIVLCFFLAAVSGISWFFPTVSWLSGFLGTPQMARLLHPFLGIAVFAGLCYMFWRFVGHN
MPARTDAIWFRRAREVLLNQHGGEPLQIGKYNAGQKVLFWLIMASILALLVSGLIMWRAYFAEYFPIPVLRLAILAHSVA
GIGLILLIVGHIYLAIWVRGSITGMVTGYVSRAWARQHHDRWYGELQAKESKAAQAKGNEA

Sequences:

>Translated_221_residues
MATNNDRILRTKFAERLCHWAIVLCFFLAAVSGISWFFPTVSWLSGFLGTPQMARLLHPFLGIAVFAGLCYMFWRFVGHN
MPARTDAIWFRRAREVLLNQHGGEPLQIGKYNAGQKVLFWLIMASILALLVSGLIMWRAYFAEYFPIPVLRLAILAHSVA
GIGLILLIVGHIYLAIWVRGSITGMVTGYVSRAWARQHHDRWYGELQAKESKAAQAKGNEA
>Mature_220_residues
ATNNDRILRTKFAERLCHWAIVLCFFLAAVSGISWFFPTVSWLSGFLGTPQMARLLHPFLGIAVFAGLCYMFWRFVGHNM
PARTDAIWFRRAREVLLNQHGGEPLQIGKYNAGQKVLFWLIMASILALLVSGLIMWRAYFAEYFPIPVLRLAILAHSVAG
IGLILLIVGHIYLAIWVRGSITGMVTGYVSRAWARQHHDRWYGELQAKESKAAQAKGNEA

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=217, Percent_Identity=53.4562211981567, Blast_Score=242, Evalue=1e-65,
Organism=Escherichia coli, GI1790325, Length=210, Percent_Identity=46.6666666666667, Blast_Score=214, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24979; Mature: 24848

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNNDRILRTKFAERLCHWAIVLCFFLAAVSGISWFFPTVSWLSGFLGTPQMARLLHPF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LGIAVFAGLCYMFWRFVGHNMPARTDAIWFRRAREVLLNQHGGEPLQIGKYNAGQKVLFW
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHH
LIMASILALLVSGLIMWRAYFAEYFPIPVLRLAILAHSVAGIGLILLIVGHIYLAIWVRG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SITGMVTGYVSRAWARQHHDRWYGELQAKESKAAQAKGNEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATNNDRILRTKFAERLCHWAIVLCFFLAAVSGISWFFPTVSWLSGFLGTPQMARLLHPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LGIAVFAGLCYMFWRFVGHNMPARTDAIWFRRAREVLLNQHGGEPLQIGKYNAGQKVLFW
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHH
LIMASILALLVSGLIMWRAYFAEYFPIPVLRLAILAHSVAGIGLILLIVGHIYLAIWVRG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SITGMVTGYVSRAWARQHHDRWYGELQAKESKAAQAKGNEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]