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The map label for this gene is cls [H]

Identifier: 116695199

GI number: 116695199

Start: 1414165

End: 1415601

Strand: Direct

Name: cls [H]

Synonym: H16_B1255

Alternate gene names: 116695199

Gene position: 1414165-1415601 (Clockwise)

Preceding gene: 116695197

Following gene: 116695200

Centisome position: 48.56

GC content: 67.85

Gene sequence:

>1437_bases
ATGCTGCAGAGCCCGAGTGTCATCGCCATCATCGTCCTGTGCTTTCACGTCGTCGGCGTGGTGGCTGCCCTGCATGCCGT
CATGACGGTGCGCACCGCGCCCGGCGCGATCGCGTGGGCGGGGTCGCTGCTGATGATGCCGTACTTCACGCTGGTGCCCT
ACCTGATCTTCGGGCGCAGCACCTTCGCCGGCTACGTCGACGCCCGGCGCTTCAATGACGACCACCTGCGCGAGCTGCGC
CACGGCATGACCCCGCGCGAGCGCGAGGCGCGCAGCGCCTGCGTGGTCCATGCGCCGGCGCAGGCCTGCATGCGTGCGCT
ACCCCGGCTGACCGGCATGCCGTGCCTGGCCAACAATGACGTGCGCTTGCTGGTCAACGGCGAGCAGACCTTCGAGGCGA
TCTTTGCCGCGATCGCGCGGGCCAGCCAGGTGCTGATCGTGCAGTTCTTCATCGTGCATGACGATGCGCTGGGCCAGCGC
CTGCAGGCGCTGCTGTGCGAGCGCGCGCGGGCCGGCGTCAAGGTGTACTTCCTGTTCGATCGCATCGGCTGCCACGCGCT
GCCGCGCCGCTACGTGCGCACGCTGGCCGAGGCCGGCGTCGATGCGCGGGCGTTCTCCACGCACCGCGGCTTTGTCAACC
GGTTTCAGCTCAATTTTCGCAACCATCGCAAGCTGGTGATCGTCGATGGCAGCGAGGCCTTTGTCGGTGGCCACAACGTC
GGCGTCGAATACCTGGGCGAACGCCCGCCACTGGCGCCGTGGCGCGACACCCATATTGCACTGCGCGGCAGCGCCGTGCT
GGACCTGCAGATGGCCTTTGCCGAAGACTGGTTCTGGGCCGCGCGCGAGGTGCCGCACCTGCTGATGCCGCCGCCAGAAG
CGGGCGGGCGCATGACCTGCCAGGTGGTGCCGTCAGGCCCTGCCGACGCGCAGGAGACCTGTTCGCTGTTCTTTGTCGAG
GCCATCCAGGCGGCGCGCCACCGGCTGTGGATCACGTCGCCGTATTTCGTGCCCGACGAGGCGGTGTTTGCGGTGCTGCG
GCTGGCCGTGCTGCGCGGCGTGGATGTGCGCATCCTGATTCCAGCGCGGCCCGATCACCTGGTGGTCTATGCCGCCTCCA
CTATCTATGCGTACCAGGCCGTGTCCGCGGGCATCAAGCTGTATCGCTACCAGCCCGGCTTCCTGCACCAGAAGGTCATC
CTGATCGACGACGAAGCCGCGGCGGTCGGCACCGCCAACCTGGACAACCGCTCGTTCCGGCTCAACTTCGAGCTGATGGT
GATGACTGCCGACAAGGGCTTTGCCGCGAGCGTGGCCACCATGCTCGAAGCCGACTTCGCCCAAGCCCGGCGCGTCGGGC
TGAACGAGTTCCTCGATGCGCCGGCGCCGCTGCGCGTGGCGATGCACGTCGCCAAGCTGTTTGCGCCCATCCTCTGA

Upstream 100 bases:

>100_bases
GGCAGCGCGCGGGCACTATGGGCGCCGTGCTGTCAACATTCGGGCAGGGTGTGCCAAAATAGCGCGCATCGCGTTCCGGT
CCGGGGACTCAAGCCCACCG

Downstream 100 bases:

>100_bases
AGCCCGCATTCCGGTCCGCTGGTTCCTGCAATTCTCAATGCAAAAGACTTGTTGCGGCCTGATTGTGTACACTGTTCACT
ATACGCGTATGCACATCATG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLQSPSVIAIIVLCFHVVGVVAALHAVMTVRTAPGAIAWAGSLLMMPYFTLVPYLIFGRSTFAGYVDARRFNDDHLRELR
HGMTPREREARSACVVHAPAQACMRALPRLTGMPCLANNDVRLLVNGEQTFEAIFAAIARASQVLIVQFFIVHDDALGQR
LQALLCERARAGVKVYFLFDRIGCHALPRRYVRTLAEAGVDARAFSTHRGFVNRFQLNFRNHRKLVIVDGSEAFVGGHNV
GVEYLGERPPLAPWRDTHIALRGSAVLDLQMAFAEDWFWAAREVPHLLMPPPEAGGRMTCQVVPSGPADAQETCSLFFVE
AIQAARHRLWITSPYFVPDEAVFAVLRLAVLRGVDVRILIPARPDHLVVYAASTIYAYQAVSAGIKLYRYQPGFLHQKVI
LIDDEAAAVGTANLDNRSFRLNFELMVMTADKGFAASVATMLEADFAQARRVGLNEFLDAPAPLRVAMHVAKLFAPIL

Sequences:

>Translated_478_residues
MLQSPSVIAIIVLCFHVVGVVAALHAVMTVRTAPGAIAWAGSLLMMPYFTLVPYLIFGRSTFAGYVDARRFNDDHLRELR
HGMTPREREARSACVVHAPAQACMRALPRLTGMPCLANNDVRLLVNGEQTFEAIFAAIARASQVLIVQFFIVHDDALGQR
LQALLCERARAGVKVYFLFDRIGCHALPRRYVRTLAEAGVDARAFSTHRGFVNRFQLNFRNHRKLVIVDGSEAFVGGHNV
GVEYLGERPPLAPWRDTHIALRGSAVLDLQMAFAEDWFWAAREVPHLLMPPPEAGGRMTCQVVPSGPADAQETCSLFFVE
AIQAARHRLWITSPYFVPDEAVFAVLRLAVLRGVDVRILIPARPDHLVVYAASTIYAYQAVSAGIKLYRYQPGFLHQKVI
LIDDEAAAVGTANLDNRSFRLNFELMVMTADKGFAASVATMLEADFAQARRVGLNEFLDAPAPLRVAMHVAKLFAPIL
>Mature_478_residues
MLQSPSVIAIIVLCFHVVGVVAALHAVMTVRTAPGAIAWAGSLLMMPYFTLVPYLIFGRSTFAGYVDARRFNDDHLRELR
HGMTPREREARSACVVHAPAQACMRALPRLTGMPCLANNDVRLLVNGEQTFEAIFAAIARASQVLIVQFFIVHDDALGQR
LQALLCERARAGVKVYFLFDRIGCHALPRRYVRTLAEAGVDARAFSTHRGFVNRFQLNFRNHRKLVIVDGSEAFVGGHNV
GVEYLGERPPLAPWRDTHIALRGSAVLDLQMAFAEDWFWAAREVPHLLMPPPEAGGRMTCQVVPSGPADAQETCSLFFVE
AIQAARHRLWITSPYFVPDEAVFAVLRLAVLRGVDVRILIPARPDHLVVYAASTIYAYQAVSAGIKLYRYQPGFLHQKVI
LIDDEAAAVGTANLDNRSFRLNFELMVMTADKGFAASVATMLEADFAQARRVGLNEFLDAPAPLRVAMHVAKLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=496, Percent_Identity=27.0161290322581, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1787007, Length=336, Percent_Identity=30.6547619047619, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52897; Mature: 52897

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSPSVIAIIVLCFHVVGVVAALHAVMTVRTAPGAIAWAGSLLMMPYFTLVPYLIFGRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TFAGYVDARRFNDDHLRELRHGMTPREREARSACVVHAPAQACMRALPRLTGMPCLANND
HHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCEEEEECCHHHHHHHHHHHCCCCEEECCC
VRLLVNGEQTFEAIFAAIARASQVLIVQFFIVHDDALGQRLQALLCERARAGVKVYFLFD
EEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEEE
RIGCHALPRRYVRTLAEAGVDARAFSTHRGFVNRFQLNFRNHRKLVIVDGSEAFVGGHNV
HHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCEEEEEECCCEEECCCCC
GVEYLGERPPLAPWRDTHIALRGSAVLDLQMAFAEDWFWAAREVPHLLMPPPEAGGRMTC
CHHCCCCCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCEEEE
QVVPSGPADAQETCSLFFVEAIQAARHRLWITSPYFVPDEAVFAVLRLAVLRGVDVRILI
EEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEE
PARPDHLVVYAASTIYAYQAVSAGIKLYRYQPGFLHQKVILIDDEAAAVGTANLDNRSFR
ECCCCEEEEEEEHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCEEE
LNFELMVMTADKGFAASVATMLEADFAQARRVGLNEFLDAPAPLRVAMHVAKLFAPIL
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQSPSVIAIIVLCFHVVGVVAALHAVMTVRTAPGAIAWAGSLLMMPYFTLVPYLIFGRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TFAGYVDARRFNDDHLRELRHGMTPREREARSACVVHAPAQACMRALPRLTGMPCLANND
HHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCEEEEECCHHHHHHHHHHHCCCCEEECCC
VRLLVNGEQTFEAIFAAIARASQVLIVQFFIVHDDALGQRLQALLCERARAGVKVYFLFD
EEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEEE
RIGCHALPRRYVRTLAEAGVDARAFSTHRGFVNRFQLNFRNHRKLVIVDGSEAFVGGHNV
HHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCEEEEEECCCEEECCCCC
GVEYLGERPPLAPWRDTHIALRGSAVLDLQMAFAEDWFWAAREVPHLLMPPPEAGGRMTC
CHHCCCCCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCEEEE
QVVPSGPADAQETCSLFFVEAIQAARHRLWITSPYFVPDEAVFAVLRLAVLRGVDVRILI
EEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEE
PARPDHLVVYAASTIYAYQAVSAGIKLYRYQPGFLHQKVILIDDEAAAVGTANLDNRSFR
ECCCCEEEEEEEHHHHHHHHHHCCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCEEE
LNFELMVMTADKGFAASVATMLEADFAQARRVGLNEFLDAPAPLRVAMHVAKLFAPIL
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA