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The map label for this gene is hupB3 [H]

Identifier: 116695179

GI number: 116695179

Start: 1395887

End: 1396168

Strand: Direct

Name: hupB3 [H]

Synonym: H16_B1235

Alternate gene names: 116695179

Gene position: 1395887-1396168 (Clockwise)

Preceding gene: 116695176

Following gene: 116695180

Centisome position: 47.93

GC content: 61.7

Gene sequence:

>282_bases
ATGAATAAAGGTGAACTGGTATCGGCCATCGCCGCGGAAGCAGAACTGAGCAATGCCGCCGCCGAGCGCGCGCTCAACGC
AGCGCTGGACAGTATCAAGAAGGCAGTGGCAAAGGGCGATTCGGTGACGCTGGTGGGCTTTGGCAGCTTCAGCGCCGGCA
AACGCGCCGCCCGCACCGGCCGCAATCCGCGCACCGGTGAAACCATCAAGATCCCGGCCGCCAAGACGGTCAAGTTCTCT
GCCGGGCAGGGTTTCAAGGACGCGGTGAACAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
GGATTGCTCTACGCGCGACGGGCAGATTAGACTGCGCCGATCGATGGCTTTGCCGCGAGGCACGCACATCGGTGACGACG
ACCAACAAGGGAAATCAGCC

Downstream 100 bases:

>100_bases
GCAGTTTGCGTCCACAGCGTGAAAAGCGGCCTTGCAGGCCGCTTTTTTGTTGCCTGGCACGGCCACGCCTGCCGGTACCG
GGCTGCAGCCGGCAACGGCG

Product: HU family DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 93; Mature: 93

Protein sequence:

>93_residues
MNKGELVSAIAAEAELSNAAAERALNAALDSIKKAVAKGDSVTLVGFGSFSAGKRAARTGRNPRTGETIKIPAAKTVKFS
AGQGFKDAVNKKK

Sequences:

>Translated_93_residues
MNKGELVSAIAAEAELSNAAAERALNAALDSIKKAVAKGDSVTLVGFGSFSAGKRAARTGRNPRTGETIKIPAAKTVKFS
AGQGFKDAVNKKK
>Mature_93_residues
MNKGELVSAIAAEAELSNAAAERALNAALDSIKKAVAKGDSVTLVGFGSFSAGKRAARTGRNPRTGETIKIPAAKTVKFS
AGQGFKDAVNKKK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=55.5555555555556, Blast_Score=98, Evalue=1e-22,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=50.561797752809, Blast_Score=89, Evalue=4e-20,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=32.967032967033, Blast_Score=64, Evalue=1e-12,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=34.8314606741573, Blast_Score=60, Evalue=2e-11,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9533; Mature: 9533

Theoretical pI: Translated: 11.07; Mature: 11.07

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGELVSAIAAEAELSNAAAERALNAALDSIKKAVAKGDSVTLVGFGSFSAGKRAARTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCC
RNPRTGETIKIPAAKTVKFSAGQGFKDAVNKKK
CCCCCCCEEECCCCCEEEECCCCCHHHHCCCCC
>Mature Secondary Structure
MNKGELVSAIAAEAELSNAAAERALNAALDSIKKAVAKGDSVTLVGFGSFSAGKRAARTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCC
RNPRTGETIKIPAAKTVKFSAGQGFKDAVNKKK
CCCCCCCEEECCCCCEEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]