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The map label for this gene is gatA [H]

Identifier: 116695117

GI number: 116695117

Start: 1325173

End: 1326699

Strand: Direct

Name: gatA [H]

Synonym: H16_B1172

Alternate gene names: 116695117

Gene position: 1325173-1326699 (Clockwise)

Preceding gene: 116695116

Following gene: 116695118

Centisome position: 45.5

GC content: 70.53

Gene sequence:

>1527_bases
ATGACAAACTCGTACCAACCGGTGCCAGACGGCCCCGGCCATCACGCGCCGTTCAGCGTGGTGGAAGCCACCGTTGCCAG
CGCGCATGCCGCGATGCGCGACGGCAGGCTGACGGCGCGCCAACTGGCCAGCGCCTGCCTCGCGCGCATCTCGGCCTATG
ATCAGGACGGTCCCGCCCTGCGCAGCATCCTGCAGCGCAACCCGCAGGCGCTGGAAGAGGCCGGCCGCATCGACGCCGTG
GCGGGCCGCAACCCCACACGGCCGCTGGCGCCGCTGCATGGCATCCCGGTGCTGGTCAAGGACAACATCGAATGCGGCGG
CATGGAAACCACCGCCGGCGCCGCCTGCCTGCGCGGCAACCTGTCCGCCGACGATGCCTTCGTCATCCGCCGGCTGCGCG
AGGCGGGTGCCATCGTCCTGGCCAAGACCAACCTGCACGAGCTTGCCTCCGGCGGCGAGACGGTCAGCACCTTGTCCGGC
CAGACCCTCAATCCCTATGACCTGACCCGCACGCCCGGCGGTTCCAGCGGCGGCACCGGCGCCGGCATCGCAGCCAGCTT
CGGCCTGCTCGGCATCGGCACCGACGGGGTCAATTCCATCCGCTCGCCGGCCTCGGCCAACAACCTGGTCGGCCTGCGTC
CGACCATGGGCCTGGTCAGCCGGGCCGGGCTGGTCCCTTGCGGGCTGACGCAGGACACCATCGGCCCGATCACCCGTACC
GTCGCCGATACGGCGCTGCTGCTCGATGTCATCGCCGGCCATGACCCGGCCGACCCGGTCACCAGCCAGGGCGCGCCCCA
CATCCCGGCGAGCTATGCCGCCAGCCTCGACCGCGACGGACTCAGGGGGGCGCGCATCGGCGTGTTGCGCCATTTCTTCG
GCGACCAGGATGTGCATCGTCCCGTCAACGCCGTGATGCAGGCAGCGCTGGCCACCATCGCTGCACAAGGCGCCGAGCTG
ATCGCGATTGAAGACGCCATCAGCCCGGACGAGCTGCTCGCGTCCACGCTTGTGCACCACTACGAGATGGAGCGCGATCT
CGATGCCTACCTGGCGGGGCTGCCCCCCGGCGTCCCGGTGCGATCGATGCAGGACATCATCGCTGCCGGTTCTGTCCATC
CAAGCGTGGCGGGCACCCTTACCACCGCGGCAGCGCTGAGCGAGCGGGAGGGGGAGTACCGCGAACGCCTGCAGCGCCAG
CAGGCATTGCGCCAGCGGCTGCAGGACCTGATGGCGCGGCATCGGCTTGACGCGCTGGCATTTCCGCACCAGCGGCGCCT
GGTGGCGCCGGTCGGCGAGACCCAGGCGGAGCGCAATGGCGTGCTCGCGTCGGCCACCGGCTTTCCGGCCATCGTGATTC
CGGCCGGATTCTCGGCGCCGGACGGCAATGCCCCCCAAGGCGTACCGGTCGGCCTGGAGTTCTTTGGCCTGCCTTTCACC
GAACCCGTGCTGATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCT
GGAATAG

Upstream 100 bases:

>100_bases
CCGATAGCGAGACAAGGCGGGGTGCGGCACCGTGCCGCTGCCCCGCCTGCCCATCATCTCGCAAACACTTCGCGCCCTTG
CCGGCGCATGGAAGGCTCTC

Downstream 100 bases:

>100_bases
GCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGACACGCGGCGACAAGTCGCGGACATAGCGCGAC
AACCTCGAAACGAGGAGGAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTNSYQPVPDGPGHHAPFSVVEATVASAHAAMRDGRLTARQLASACLARISAYDQDGPALRSILQRNPQALEEAGRIDAV
AGRNPTRPLAPLHGIPVLVKDNIECGGMETTAGAACLRGNLSADDAFVIRRLREAGAIVLAKTNLHELASGGETVSTLSG
QTLNPYDLTRTPGGSSGGTGAGIAASFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRAGLVPCGLTQDTIGPITRT
VADTALLLDVIAGHDPADPVTSQGAPHIPASYAASLDRDGLRGARIGVLRHFFGDQDVHRPVNAVMQAALATIAAQGAEL
IAIEDAISPDELLASTLVHHYEMERDLDAYLAGLPPGVPVRSMQDIIAAGSVHPSVAGTLTTAAALSEREGEYRERLQRQ
QALRQRLQDLMARHRLDALAFPHQRRLVAPVGETQAERNGVLASATGFPAIVIPAGFSAPDGNAPQGVPVGLEFFGLPFT
EPVLIRLAYAAEQALRARRPPHSTPALE

Sequences:

>Translated_508_residues
MTNSYQPVPDGPGHHAPFSVVEATVASAHAAMRDGRLTARQLASACLARISAYDQDGPALRSILQRNPQALEEAGRIDAV
AGRNPTRPLAPLHGIPVLVKDNIECGGMETTAGAACLRGNLSADDAFVIRRLREAGAIVLAKTNLHELASGGETVSTLSG
QTLNPYDLTRTPGGSSGGTGAGIAASFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRAGLVPCGLTQDTIGPITRT
VADTALLLDVIAGHDPADPVTSQGAPHIPASYAASLDRDGLRGARIGVLRHFFGDQDVHRPVNAVMQAALATIAAQGAEL
IAIEDAISPDELLASTLVHHYEMERDLDAYLAGLPPGVPVRSMQDIIAAGSVHPSVAGTLTTAAALSEREGEYRERLQRQ
QALRQRLQDLMARHRLDALAFPHQRRLVAPVGETQAERNGVLASATGFPAIVIPAGFSAPDGNAPQGVPVGLEFFGLPFT
EPVLIRLAYAAEQALRARRPPHSTPALE
>Mature_507_residues
TNSYQPVPDGPGHHAPFSVVEATVASAHAAMRDGRLTARQLASACLARISAYDQDGPALRSILQRNPQALEEAGRIDAVA
GRNPTRPLAPLHGIPVLVKDNIECGGMETTAGAACLRGNLSADDAFVIRRLREAGAIVLAKTNLHELASGGETVSTLSGQ
TLNPYDLTRTPGGSSGGTGAGIAASFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRAGLVPCGLTQDTIGPITRTV
ADTALLLDVIAGHDPADPVTSQGAPHIPASYAASLDRDGLRGARIGVLRHFFGDQDVHRPVNAVMQAALATIAAQGAELI
AIEDAISPDELLASTLVHHYEMERDLDAYLAGLPPGVPVRSMQDIIAAGSVHPSVAGTLTTAAALSEREGEYRERLQRQQ
ALRQRLQDLMARHRLDALAFPHQRRLVAPVGETQAERNGVLASATGFPAIVIPAGFSAPDGNAPQGVPVGLEFFGLPFTE
PVLIRLAYAAEQALRARRPPHSTPALE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=24.5059288537549, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=25.5102040816327, Blast_Score=111, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=446, Percent_Identity=26.9058295964126, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=275, Percent_Identity=30.1818181818182, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=252, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=272, Percent_Identity=28.3088235294118, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=225, Percent_Identity=28.4444444444444, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=410, Percent_Identity=26.0975609756098, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=238, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550774, Length=302, Percent_Identity=34.4370860927152, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648435, Length=302, Percent_Identity=34.4370860927152, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648437, Length=302, Percent_Identity=34.4370860927152, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648441, Length=239, Percent_Identity=35.9832635983264, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648439, Length=239, Percent_Identity=35.9832635983264, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24652985, Length=473, Percent_Identity=26.6384778012685, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922090, Length=473, Percent_Identity=26.6384778012685, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652981, Length=473, Percent_Identity=26.6384778012685, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652983, Length=473, Percent_Identity=26.6384778012685, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=25.4582484725051, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648113, Length=258, Percent_Identity=34.4961240310078, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=24.4186046511628, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=28.0392156862745, Blast_Score=97, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53003; Mature: 52871

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSYQPVPDGPGHHAPFSVVEATVASAHAAMRDGRLTARQLASACLARISAYDQDGPAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
RSILQRNPQALEEAGRIDAVAGRNPTRPLAPLHGIPVLVKDNIECGGMETTAGAACLRGN
HHHHHCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCC
LSADDAFVIRRLREAGAIVLAKTNLHELASGGETVSTLSGQTLNPYDLTRTPGGSSGGTG
CCCCHHHHHHHHHHCCEEEEEECCHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCC
AGIAASFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRAGLVPCGLTQDTIGPITRT
CHHHHHCCEEEECCCCHHHHCCCCCCCCCEECCHHHHHHHHCCCCCCCCCHHHHHHHHHH
VADTALLLDVIAGHDPADPVTSQGAPHIPASYAASLDRDGLRGARIGVLRHFFGDQDVHR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHH
PVNAVMQAALATIAAQGAELIAIEDAISPDELLASTLVHHYEMERDLDAYLAGLPPGVPV
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RSMQDIIAAGSVHPSVAGTLTTAAALSEREGEYRERLQRQQALRQRLQDLMARHRLDALA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPHQRRLVAPVGETQAERNGVLASATGFPAIVIPAGFSAPDGNAPQGVPVGLEFFGLPFT
CCCCCCEECCCCCCHHHHCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHEECCCCC
EPVLIRLAYAAEQALRARRPPHSTPALE
CHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TNSYQPVPDGPGHHAPFSVVEATVASAHAAMRDGRLTARQLASACLARISAYDQDGPAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
RSILQRNPQALEEAGRIDAVAGRNPTRPLAPLHGIPVLVKDNIECGGMETTAGAACLRGN
HHHHHCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCC
LSADDAFVIRRLREAGAIVLAKTNLHELASGGETVSTLSGQTLNPYDLTRTPGGSSGGTG
CCCCHHHHHHHHHHCCEEEEEECCHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCC
AGIAASFGLLGIGTDGVNSIRSPASANNLVGLRPTMGLVSRAGLVPCGLTQDTIGPITRT
CHHHHHCCEEEECCCCHHHHCCCCCCCCCEECCHHHHHHHHCCCCCCCCCHHHHHHHHHH
VADTALLLDVIAGHDPADPVTSQGAPHIPASYAASLDRDGLRGARIGVLRHFFGDQDVHR
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHH
PVNAVMQAALATIAAQGAELIAIEDAISPDELLASTLVHHYEMERDLDAYLAGLPPGVPV
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RSMQDIIAAGSVHPSVAGTLTTAAALSEREGEYRERLQRQQALRQRLQDLMARHRLDALA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPHQRRLVAPVGETQAERNGVLASATGFPAIVIPAGFSAPDGNAPQGVPVGLEFFGLPFT
CCCCCCEECCCCCCHHHHCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHEECCCCC
EPVLIRLAYAAEQALRARRPPHSTPALE
CHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA