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The map label for this gene is lpdA [H]

Identifier: 116695043

GI number: 116695043

Start: 1242169

End: 1243560

Strand: Direct

Name: lpdA [H]

Synonym: H16_B1098

Alternate gene names: 116695043

Gene position: 1242169-1243560 (Clockwise)

Preceding gene: 116695042

Following gene: 116695044

Centisome position: 42.65

GC content: 69.32

Gene sequence:

>1392_bases
ATGACCACCATCCAGACCGACGTCGCCGTGATCGGCGCAGGCACCGCCGGCCTTGCCGCCTACCGTGCCGCGATTGCCGC
GGGCAAGCGCGCCGTGATCATCGAAGGCGGCCCGTACGGCACCACCTGCGCCCGGGTGGGCTGCATGCCCTCCAAGCTGC
TGATCGCTGCCGCCGAGGCCGCGCACGAACTGCGCCACACTGCGCCGTTCGGCGTGCACGTGGATGGCGAGGTCCGCATC
GATGGCCGCGAGGTGATGGCCCGCGTGCGCAGCGAGCGCGACCGCTTTGTCGGCTTCGTGGTGCGCGGCGTGGAGAACAT
CCCCGAAGCCGACCGCGTCCGCGGCTATGCCCGCTTTATCGACAACACCACGCTGGAAGTGGATGGGCACACCACCGTGC
GTGCCAGCCGCGTGGTCATTGCCACCGGCTCCACGCCGATGCTGCCGGCGCCGTTCCAGGTGTTCGGCGACCGCCTGATC
GTCAATGACGATGTGTTCGCTTGGGAAGACCTGCCCGCCAGCGTGGTGGCGTTCGGCCCCGGCGTGATCGGGCTGGAGCT
TGGCCAGGCCTTGTCGCGCCTTGGCGTGCGGGTGCGCGTGTTCGGCGTCAGCGGCTCGCTCGGGCCGCTGACTGATCCCG
TGATCCGCGCCTACGCGGCGAAGACCTTCAACGGCGAGTTCTATCTCGATCCGGACGCCAGGGTGACCGACATGGCCCGC
GACGGCGACCAGGTGGTGGTGACTTACCTGGACCGCGAAGGCCGCAGCGTGACCGAGCGCTTCGACTATGCGCTGGTCGC
CACCGGCCGCGTGCCCAATGTGCGCGGCCTGGGCCTGGAAAATGCCGGCCTGGCGCTGGATGCGCGCGGCGTGCCGCTGT
TCGATGCGCGCACGCTCCAGTGCGGCAATGCACCGGTATTCATTGCCGGCGACGCCAACAACATCCTGCCGCTGCTGCAT
GAGGCCGCGGACGAGGGCAAGGCCGCGGGCGAGAACGCCGCCAGCTATCCGCAAGTGAAGCCGCTGGCACGGCGCGCGCC
GATCGCGGTGGTGTTCACGGACCCGCAGATCGCGATGGTCGGCCAGCGTTACGCCGACCTGGCCGAAGGCACGTTCGTGA
CCGGCGAGGTCAGCTTCGAGGACCAGGGCCGCAGCCGCGTGATGCTGAAGAACCGCGGCCTGATGCATGTCTATGCCGAC
AAGGCCACGGGCCGCTTCCTGGGCGCGGAATGGACCGGCCCGCGTGCCGAGAACATCGCCCACCTGCTGGCGTGGTCGTA
CCAGCAGGGCCTGACCATCGGGCAGATGCTGGGCATGCCTTTCTACCACCCGGTGGTGGAAGAGGGCCTGCGTACGGCGC
TGCGTGATGCGGAGGCGAAGCTGGAGGCCTGA

Upstream 100 bases:

>100_bases
TCGCCGGACCCGATGGGGTCGCCTTGCCCGGCATGGCGGCCCCATCCAGCCCGCTGATTGACACCAGCTTGACGCCATCA
AGACAAAGGAATTGACCGCC

Downstream 100 bases:

>100_bases
GCGGGCCAGGTTTGCTAGACAGGTCTGTCTGTCATAGTATCGACCTATAGAGAGGCAGGGCGGCGCAGGGCGCTGTCCGG
CCGCGCACCTTTACCGACTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHELRHTAPFGVHVDGEVRI
DGREVMARVRSERDRFVGFVVRGVENIPEADRVRGYARFIDNTTLEVDGHTTVRASRVVIATGSTPMLPAPFQVFGDRLI
VNDDVFAWEDLPASVVAFGPGVIGLELGQALSRLGVRVRVFGVSGSLGPLTDPVIRAYAAKTFNGEFYLDPDARVTDMAR
DGDQVVVTYLDREGRSVTERFDYALVATGRVPNVRGLGLENAGLALDARGVPLFDARTLQCGNAPVFIAGDANNILPLLH
EAADEGKAAGENAASYPQVKPLARRAPIAVVFTDPQIAMVGQRYADLAEGTFVTGEVSFEDQGRSRVMLKNRGLMHVYAD
KATGRFLGAEWTGPRAENIAHLLAWSYQQGLTIGQMLGMPFYHPVVEEGLRTALRDAEAKLEA

Sequences:

>Translated_463_residues
MTTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHELRHTAPFGVHVDGEVRI
DGREVMARVRSERDRFVGFVVRGVENIPEADRVRGYARFIDNTTLEVDGHTTVRASRVVIATGSTPMLPAPFQVFGDRLI
VNDDVFAWEDLPASVVAFGPGVIGLELGQALSRLGVRVRVFGVSGSLGPLTDPVIRAYAAKTFNGEFYLDPDARVTDMAR
DGDQVVVTYLDREGRSVTERFDYALVATGRVPNVRGLGLENAGLALDARGVPLFDARTLQCGNAPVFIAGDANNILPLLH
EAADEGKAAGENAASYPQVKPLARRAPIAVVFTDPQIAMVGQRYADLAEGTFVTGEVSFEDQGRSRVMLKNRGLMHVYAD
KATGRFLGAEWTGPRAENIAHLLAWSYQQGLTIGQMLGMPFYHPVVEEGLRTALRDAEAKLEA
>Mature_462_residues
TTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHELRHTAPFGVHVDGEVRID
GREVMARVRSERDRFVGFVVRGVENIPEADRVRGYARFIDNTTLEVDGHTTVRASRVVIATGSTPMLPAPFQVFGDRLIV
NDDVFAWEDLPASVVAFGPGVIGLELGQALSRLGVRVRVFGVSGSLGPLTDPVIRAYAAKTFNGEFYLDPDARVTDMARD
GDQVVVTYLDREGRSVTERFDYALVATGRVPNVRGLGLENAGLALDARGVPLFDARTLQCGNAPVFIAGDANNILPLLHE
AADEGKAAGENAASYPQVKPLARRAPIAVVFTDPQIAMVGQRYADLAEGTFVTGEVSFEDQGRSRVMLKNRGLMHVYADK
ATGRFLGAEWTGPRAENIAHLLAWSYQQGLTIGQMLGMPFYHPVVEEGLRTALRDAEAKLEA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=27.1367521367521, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=25.1072961373391, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI22035672, Length=295, Percent_Identity=28.135593220339, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786307, Length=465, Percent_Identity=27.5268817204301, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87082354, Length=460, Percent_Identity=25.2173913043478, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789915, Length=346, Percent_Identity=28.0346820809249, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=23.644251626898, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=28.2051282051282, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=482, Percent_Identity=25.3112033195021, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=344, Percent_Identity=27.6162790697674, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71983429, Length=344, Percent_Identity=27.6162790697674, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=26.890756302521, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=326, Percent_Identity=24.8466257668712, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325240, Length=499, Percent_Identity=23.8476953907816, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=28.7846481876333, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=25.4237288135593, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=25.4736842105263, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=25.1612903225806, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17737741, Length=498, Percent_Identity=25.5020080321285, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49615; Mature: 49484

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEA
CCEEECCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
AHELRHTAPFGVHVDGEVRIDGREVMARVRSERDRFVGFVVRGVENIPEADRVRGYARFI
HHHHHHCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DNTTLEVDGHTTVRASRVVIATGSTPMLPAPFQVFGDRLIVNDDVFAWEDLPASVVAFGP
CCCEEEECCCEEEEEEEEEEEECCCCCCCCCHHHCCCEEEEECCCEEECCCCCEEEEECC
GVIGLELGQALSRLGVRVRVFGVSGSLGPLTDPVIRAYAAKTFNGEFYLDPDARVTDMAR
CEEHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHEEECCEEEECCCCCEEHHCC
DGDQVVVTYLDREGRSVTERFDYALVATGRVPNVRGLGLENAGLALDARGVPLFDARTLQ
CCCEEEEEEECCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEECCCEEE
CGNAPVFIAGDANNILPLLHEAADEGKAAGENAASYPQVKPLARRAPIAVVFTDPQIAMV
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCEEEH
GQRYADLAEGTFVTGEVSFEDQGRSRVMLKNRGLMHVYADKATGRFLGAEWTGPRAENIA
HHHHHHHHCCEEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCEECCCCCCCCHHHHH
HLLAWSYQQGLTIGQMLGMPFYHPVVEEGLRTALRDAEAKLEA
HHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEA
CEEECCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
AHELRHTAPFGVHVDGEVRIDGREVMARVRSERDRFVGFVVRGVENIPEADRVRGYARFI
HHHHHHCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DNTTLEVDGHTTVRASRVVIATGSTPMLPAPFQVFGDRLIVNDDVFAWEDLPASVVAFGP
CCCEEEECCCEEEEEEEEEEEECCCCCCCCCHHHCCCEEEEECCCEEECCCCCEEEEECC
GVIGLELGQALSRLGVRVRVFGVSGSLGPLTDPVIRAYAAKTFNGEFYLDPDARVTDMAR
CEEHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHEEECCEEEECCCCCEEHHCC
DGDQVVVTYLDREGRSVTERFDYALVATGRVPNVRGLGLENAGLALDARGVPLFDARTLQ
CCCEEEEEEECCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEECCCEEE
CGNAPVFIAGDANNILPLLHEAADEGKAAGENAASYPQVKPLARRAPIAVVFTDPQIAMV
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCEEEH
GQRYADLAEGTFVTGEVSFEDQGRSRVMLKNRGLMHVYADKATGRFLGAEWTGPRAENIA
HHHHHHHHCCEEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCEECCCCCCCCHHHHH
HLLAWSYQQGLTIGQMLGMPFYHPVVEEGLRTALRDAEAKLEA
HHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339281; 1581034 [H]