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The map label for this gene is garD [H]

Identifier: 116694910

GI number: 116694910

Start: 1088097

End: 1089662

Strand: Direct

Name: garD [H]

Synonym: H16_B0965

Alternate gene names: 116694910

Gene position: 1088097-1089662 (Clockwise)

Preceding gene: 116694907

Following gene: 116694913

Centisome position: 37.36

GC content: 67.56

Gene sequence:

>1566_bases
ATGACAGTGACCCCGACAGCAGGTGGACAGGCGGCTCGCCCGCTGTACATCACGATGCACGCGAACGACAACGTCGCGAT
CGTGGCCAACGACGGCGGCCTGCCGGCGGGAATGGAATTCCCCTGCGGCCTGGTGCTGGCCGAGGGCGTACCCCAGGGCC
ACAAGGTGGCGCTGACTGACCTGCGCGAGGGCGACGCGGTGATCCGCTACAACGTGGTGATCGGCTATGCTCTGAAAGAC
CTGCCGCGCGGCAGCTGGGTCAATGAGCGCGTGATCCGCATGCCGGCCGCGCCGGAACTGCACGACCTGCCGGTCGGCAC
GCGCGTGGCGCCGCTGCTACCGCTGGAAGGCTATACCTTCGAGGGCTACCGCAATGCGGACGGCTCGGTTGGCACACGCA
ATATCCTGGGCATCACCACTACGGTGCAGTGCGTGGAGGGCGTGGTGGAGTTTGCCGTGCGGCGCATCAAGGAAGAACTT
CTGCCGAAGTATCCCAATGTCGACGACGTGGTGGGCCTTGAGCACACCTACGGCTGCGGCGTGGCAATCGACGCGCCCGA
TGCCGGCATCCCGATCCGCACCATCCGCAATATCGCGCTGAACCCCAATTTCGGCGGCGAGGTGATGATCGTCAGCCTGG
GTTGCGAGAAGCTGCAGCCGGCGCGCCTGATCGGCGCCGGCACCATCCCGATCCAGCAGGAGGGCGAGCCCGACGTGGTC
TGCCTGCAGGACGAGCAGCACGTGGGCTTTGCCTCGATGATCGATTCGATCATGGCCACGGCAGAGAAGCATCTGGCCCG
CCTGAACGCGCGCCGGCGCGAGACCTGTCCGGCGTCAGACCTGGTGGTCGGCGTGCAGTGCGGCGGCAGCGATGCCTTCT
CCGGCGTCACCGCCAACCCCGCGGTGGGCTTTGCCACCGACCTGCTGGTGCGCGCCGGGGCCACGGTGATGTTCTCCGAA
GTGACCGAGGTGCGCGACGGCATCGACCAACTGACCGCGCGCGCGGCCACCCCCGAGGTGGCCGAGGCGCTGGTGCGGCA
GATGGCCTGGTACGACCGCTACCTGGAGCAGGGCAAGGTCGACCGCAGCGCCAACACCACGCCGGGCAACAAGAAGGGCG
GCCTGTCGAACATCGTGGAAAAGGCGATGGGCTCGATTGTCAAGTCGGGCACCGGCCCGATCCACGGCGTCACCGGCCCC
GGCGAGAAGGTCACGCAGAAGGGGCTGATCTACGCCGCCACGCCCGCGGGCGATTTTGTCTGCGGCACGCTGCAGCTGGC
CGCCGGCATGAACCTGCACGTGTTCACCACCGGGCGCGGCACGCCGTACGGGCTGGCCGAGGTGCCGGTGATCAAGGTGT
CCACCCGCAATGACCTGGCACGGCGCTGGCATGACCTGATGGATGTCAACGCCGGCCGCATCGCGACCGGCGAGGCCACC
ATCGAGGAAGTGGGCTGGGAGCTGTTCCACACGCTCCTGGCCGTGGCCAGTGGCAAGAAGTCATGGGCCGAGCACTGGAA
GATCCGCAATGCGCTGGTGCTGTTCAACCCGGCACCGGTGACCTGA

Upstream 100 bases:

>100_bases
AATAGGGAATTCCCGGGGCCGTAGACCACCATAAACGGATGGCAAGTTGTCAGACAACGTAACACTAAAAGTGCGCAACG
CGCACCCGGCTGGAGATGAC

Downstream 100 bases:

>100_bases
GCCTCGGCTCTGACGGCGCTAGGTGCGCCCGCGCAACGGCCTGAGCAGATGCGACAGGCCGTTGTGGTCCAGCTCATGCA
TCAGCGCCAGCAGCGCGCCG

Product: D-galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTVTPTAGGQAARPLYITMHANDNVAIVANDGGLPAGMEFPCGLVLAEGVPQGHKVALTDLREGDAVIRYNVVIGYALKD
LPRGSWVNERVIRMPAAPELHDLPVGTRVAPLLPLEGYTFEGYRNADGSVGTRNILGITTTVQCVEGVVEFAVRRIKEEL
LPKYPNVDDVVGLEHTYGCGVAIDAPDAGIPIRTIRNIALNPNFGGEVMIVSLGCEKLQPARLIGAGTIPIQQEGEPDVV
CLQDEQHVGFASMIDSIMATAEKHLARLNARRRETCPASDLVVGVQCGGSDAFSGVTANPAVGFATDLLVRAGATVMFSE
VTEVRDGIDQLTARAATPEVAEALVRQMAWYDRYLEQGKVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGTGPIHGVTGP
GEKVTQKGLIYAATPAGDFVCGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVSTRNDLARRWHDLMDVNAGRIATGEAT
IEEVGWELFHTLLAVASGKKSWAEHWKIRNALVLFNPAPVT

Sequences:

>Translated_521_residues
MTVTPTAGGQAARPLYITMHANDNVAIVANDGGLPAGMEFPCGLVLAEGVPQGHKVALTDLREGDAVIRYNVVIGYALKD
LPRGSWVNERVIRMPAAPELHDLPVGTRVAPLLPLEGYTFEGYRNADGSVGTRNILGITTTVQCVEGVVEFAVRRIKEEL
LPKYPNVDDVVGLEHTYGCGVAIDAPDAGIPIRTIRNIALNPNFGGEVMIVSLGCEKLQPARLIGAGTIPIQQEGEPDVV
CLQDEQHVGFASMIDSIMATAEKHLARLNARRRETCPASDLVVGVQCGGSDAFSGVTANPAVGFATDLLVRAGATVMFSE
VTEVRDGIDQLTARAATPEVAEALVRQMAWYDRYLEQGKVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGTGPIHGVTGP
GEKVTQKGLIYAATPAGDFVCGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVSTRNDLARRWHDLMDVNAGRIATGEAT
IEEVGWELFHTLLAVASGKKSWAEHWKIRNALVLFNPAPVT
>Mature_520_residues
TVTPTAGGQAARPLYITMHANDNVAIVANDGGLPAGMEFPCGLVLAEGVPQGHKVALTDLREGDAVIRYNVVIGYALKDL
PRGSWVNERVIRMPAAPELHDLPVGTRVAPLLPLEGYTFEGYRNADGSVGTRNILGITTTVQCVEGVVEFAVRRIKEELL
PKYPNVDDVVGLEHTYGCGVAIDAPDAGIPIRTIRNIALNPNFGGEVMIVSLGCEKLQPARLIGAGTIPIQQEGEPDVVC
LQDEQHVGFASMIDSIMATAEKHLARLNARRRETCPASDLVVGVQCGGSDAFSGVTANPAVGFATDLLVRAGATVMFSEV
TEVRDGIDQLTARAATPEVAEALVRQMAWYDRYLEQGKVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGTGPIHGVTGPG
EKVTQKGLIYAATPAGDFVCGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVSTRNDLARRWHDLMDVNAGRIATGEATI
EEVGWELFHTLLAVASGKKSWAEHWKIRNALVLFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=525, Percent_Identity=64.5714285714286, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=523, Percent_Identity=33.2695984703633, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 55407; Mature: 55276

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTPTAGGQAARPLYITMHANDNVAIVANDGGLPAGMEFPCGLVLAEGVPQGHKVALTD
CEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEEEEE
LREGDAVIRYNVVIGYALKDLPRGSWVNERVIRMPAAPELHDLPVGTRVAPLLPLEGYTF
CCCCCEEEEEEEEEEEHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
EGYRNADGSVGTRNILGITTTVQCVEGVVEFAVRRIKEELLPKYPNVDDVVGLEHTYGCG
CCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VAIDAPDAGIPIRTIRNIALNPNFGGEVMIVSLGCEKLQPARLIGAGTIPIQQEGEPDVV
EEECCCCCCCCHHHHHHEEECCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCEE
CLQDEQHVGFASMIDSIMATAEKHLARLNARRRETCPASDLVVGVQCGGSDAFSGVTANP
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
AVGFATDLLVRAGATVMFSEVTEVRDGIDQLTARAATPEVAEALVRQMAWYDRYLEQGKV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
DRSANTTPGNKKGGLSNIVEKAMGSIVKSGTGPIHGVTGPGEKVTQKGLIYAATPAGDFV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCEEEEECCCCCEE
CGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVSTRNDLARRWHDLMDVNAGRIATGEAT
EEHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEECCCHH
IEEVGWELFHTLLAVASGKKSWAEHWKIRNALVLFNPAPVT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCC
>Mature Secondary Structure 
TVTPTAGGQAARPLYITMHANDNVAIVANDGGLPAGMEFPCGLVLAEGVPQGHKVALTD
EECCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEEEEE
LREGDAVIRYNVVIGYALKDLPRGSWVNERVIRMPAAPELHDLPVGTRVAPLLPLEGYTF
CCCCCEEEEEEEEEEEHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
EGYRNADGSVGTRNILGITTTVQCVEGVVEFAVRRIKEELLPKYPNVDDVVGLEHTYGCG
CCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
VAIDAPDAGIPIRTIRNIALNPNFGGEVMIVSLGCEKLQPARLIGAGTIPIQQEGEPDVV
EEECCCCCCCCHHHHHHEEECCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCEE
CLQDEQHVGFASMIDSIMATAEKHLARLNARRRETCPASDLVVGVQCGGSDAFSGVTANP
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
AVGFATDLLVRAGATVMFSEVTEVRDGIDQLTARAATPEVAEALVRQMAWYDRYLEQGKV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
DRSANTTPGNKKGGLSNIVEKAMGSIVKSGTGPIHGVTGPGEKVTQKGLIYAATPAGDFV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCEEEEECCCCCEE
CGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVSTRNDLARRWHDLMDVNAGRIATGEAT
EEHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEECCCHH
IEEVGWELFHTLLAVASGKKSWAEHWKIRNALVLFNPAPVT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]