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The map label for this gene is thcD [H]

Identifier: 116694890

GI number: 116694890

Start: 1064553

End: 1066085

Strand: Direct

Name: thcD [H]

Synonym: H16_B0945

Alternate gene names: 116694890

Gene position: 1064553-1066085 (Clockwise)

Preceding gene: 116694884

Following gene: 116694892

Centisome position: 36.55

GC content: 66.14

Gene sequence:

>1533_bases
ATGACCAAAAGCTCTTCCTCCACGGATAAGCCCGATCTCACCCAGGGCGTGCCGCTGGCTGACCTTGCTGACGGCGGCAT
GGTCACAGGCCAGGTTGGCGGCAAACCGGTCTTGCTTGTGCGTCGGGCTGATGAACTGTTTGCCATCGGTGCGACCTGTA
CCCACTATGGCGGGCCACTGGCGGACGGCCTGCTGGTCGGCGATACGATCCGATGTCCGTGGCATCACGCCTGCTTCAGT
CTTCGCACCGGCGAGGTCCTCGGGCCGCCCGCCCAGGACGATCTGAAATGCTGGCGCGTGGAACAGCAAGACGGGAAGGC
CTTCGTCCGCGACGCGCTGCCGGCGGTGCGGCCACCGAGGCTTGCCTCGGCCCGGCTCCCTGAATCCGTGGTGATCATCG
GCGGCGGCGCCGCCGGGACTGCCGCGGTCGAAACCCTGCGGCGCGAAGGCTATAGCGGCCCCGTCACGCTGCTGAGTGCC
GATCGCTCATTGCCGTACGATCGGCCGAATCTGTCGAAAGACTACCTCGCCGGGACAGCAAACGCGGACTGGCTGCCAAT
GCGGCCACCGACGTTCTACGCGGATCACGACATCGACGTGCGCTCCGACAACCGTGTCGTCAAGCTCAGCCCCGAGCAAA
AATCCGTCACGCTCTCGGACGGCAGCAACGTGAGCTACGGCGCGCTTCTGCTTGCCGTCGGTGCCGTGCCGATACGGCTG
GACGTTCCTGGCGCAACCTTGCCGCACGTGAGCGTGCTCCGCACGCTGGCCGACTGCGACGCCCTGATCGCGCGTCTCGG
CACGGCACGCCGATGCGTCGTGGTGGGCGCAAGCTTTATCGGGATGGAAGTCGCGGCAGCGCTGCGAACGCGAGGACTGG
AAGTGCATGTTGTCGCGCCCGAAGCGCATCCGATGGAGCGGGTCCTGGGCGCGACGCTCGGCGACATGATCAAGGCGCTT
CACGAATCGCACGGGGTAACGTTCCATCTCGGGGCCACGGTGGCAGAGATTCAGCCGGATCGGGTGAGATTGTCGACCGG
CGCGGAGCTGGCCGCCGACCTGGTCGTGACCGGGATCGGCGTTCGCCCCGACGTGGCCTTGGCGCAGGACGCCGGCCTCG
CACTGGACAAGGGCGTGGCGGTCGATGAATTCCTCCAGACCAGCGAACCTGACATCTATGCCGCGGGCGATATTGCGCGC
TGGCCCGACCCGGGAACGGGCCAGCGTATCCGCGTCGAACACTGGGTGGTCGCCGAGCGCCAGGGCGTGGTGGCAGCGCG
AAATATCCTGGGACAGCGGCAACGCTTTGCGGCCGTGCCATTCTTCTGGACCCAGCACTATGATGTGGCCATCAACTACG
TAGGCCACGCGGAGCAATGGGATCGTTTCGATGTCGACGGCGATCCCGCCGCGCATGACTGTACGGTGACCTATTGGGGC
AAGGATAAAAGGCTTGCGGTCGCCACGGTCGGGCGCGACCTGGCGAGCCTTCGCGCGGAGGTGGCGTTCGAGCAGGAGAC
GACAGGACACTAG

Upstream 100 bases:

>100_bases
ATCCGTGCCGTCGCGCAGTTTGTTACGCTGCATGCGCCTTTCATTTGGCGGCCTCGAGGTCGATAATGGCCCTGTGCCAT
GACCGGCAAGGAGAGACGCA

Downstream 100 bases:

>100_bases
GCAGGGGCGAGTGCGCGGTCCGCAAGCCCTTCAAGCAGGTCGGCCGACGGGACAAAGAACAGGCTGCCGGTGACCGCCTT
GCTGAAGTCAAGCAGCCGGT

Product: pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTKSSSSTDKPDLTQGVPLADLADGGMVTGQVGGKPVLLVRRADELFAIGATCTHYGGPLADGLLVGDTIRCPWHHACFS
LRTGEVLGPPAQDDLKCWRVEQQDGKAFVRDALPAVRPPRLASARLPESVVIIGGGAAGTAAVETLRREGYSGPVTLLSA
DRSLPYDRPNLSKDYLAGTANADWLPMRPPTFYADHDIDVRSDNRVVKLSPEQKSVTLSDGSNVSYGALLLAVGAVPIRL
DVPGATLPHVSVLRTLADCDALIARLGTARRCVVVGASFIGMEVAAALRTRGLEVHVVAPEAHPMERVLGATLGDMIKAL
HESHGVTFHLGATVAEIQPDRVRLSTGAELAADLVVTGIGVRPDVALAQDAGLALDKGVAVDEFLQTSEPDIYAAGDIAR
WPDPGTGQRIRVEHWVVAERQGVVAARNILGQRQRFAAVPFFWTQHYDVAINYVGHAEQWDRFDVDGDPAAHDCTVTYWG
KDKRLAVATVGRDLASLRAEVAFEQETTGH

Sequences:

>Translated_510_residues
MTKSSSSTDKPDLTQGVPLADLADGGMVTGQVGGKPVLLVRRADELFAIGATCTHYGGPLADGLLVGDTIRCPWHHACFS
LRTGEVLGPPAQDDLKCWRVEQQDGKAFVRDALPAVRPPRLASARLPESVVIIGGGAAGTAAVETLRREGYSGPVTLLSA
DRSLPYDRPNLSKDYLAGTANADWLPMRPPTFYADHDIDVRSDNRVVKLSPEQKSVTLSDGSNVSYGALLLAVGAVPIRL
DVPGATLPHVSVLRTLADCDALIARLGTARRCVVVGASFIGMEVAAALRTRGLEVHVVAPEAHPMERVLGATLGDMIKAL
HESHGVTFHLGATVAEIQPDRVRLSTGAELAADLVVTGIGVRPDVALAQDAGLALDKGVAVDEFLQTSEPDIYAAGDIAR
WPDPGTGQRIRVEHWVVAERQGVVAARNILGQRQRFAAVPFFWTQHYDVAINYVGHAEQWDRFDVDGDPAAHDCTVTYWG
KDKRLAVATVGRDLASLRAEVAFEQETTGH
>Mature_509_residues
TKSSSSTDKPDLTQGVPLADLADGGMVTGQVGGKPVLLVRRADELFAIGATCTHYGGPLADGLLVGDTIRCPWHHACFSL
RTGEVLGPPAQDDLKCWRVEQQDGKAFVRDALPAVRPPRLASARLPESVVIIGGGAAGTAAVETLRREGYSGPVTLLSAD
RSLPYDRPNLSKDYLAGTANADWLPMRPPTFYADHDIDVRSDNRVVKLSPEQKSVTLSDGSNVSYGALLLAVGAVPIRLD
VPGATLPHVSVLRTLADCDALIARLGTARRCVVVGASFIGMEVAAALRTRGLEVHVVAPEAHPMERVLGATLGDMIKALH
ESHGVTFHLGATVAEIQPDRVRLSTGAELAADLVVTGIGVRPDVALAQDAGLALDKGVAVDEFLQTSEPDIYAAGDIARW
PDPGTGQRIRVEHWVVAERQGVVAARNILGQRQRFAAVPFFWTQHYDVAINYVGHAEQWDRFDVDGDPAAHDCTVTYWGK
DKRLAVATVGRDLASLRAEVAFEQETTGH

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=503, Percent_Identity=34.7912524850895, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI65787454, Length=503, Percent_Identity=34.7912524850895, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI226437568, Length=503, Percent_Identity=34.7912524850895, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI4757732, Length=328, Percent_Identity=28.3536585365854, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI22202629, Length=328, Percent_Identity=28.3536585365854, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1788892, Length=331, Percent_Identity=28.7009063444109, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=30.6338028169014, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789065, Length=310, Percent_Identity=27.741935483871, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=505, Percent_Identity=30.8910891089109, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI32564386, Length=373, Percent_Identity=26.0053619302949, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281359715, Length=478, Percent_Identity=34.1004184100418, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI281359713, Length=478, Percent_Identity=34.1004184100418, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24639250, Length=478, Percent_Identity=34.1004184100418, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI18543267, Length=478, Percent_Identity=34.1004184100418, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24639252, Length=478, Percent_Identity=34.1004184100418, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24639257, Length=478, Percent_Identity=34.1004184100418, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24585130, Length=476, Percent_Identity=30.0420168067227, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24581020, Length=374, Percent_Identity=29.9465240641711, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI28573993, Length=374, Percent_Identity=29.9465240641711, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 54470; Mature: 54339

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSSSSTDKPDLTQGVPLADLADGGMVTGQVGGKPVLLVRRADELFAIGATCTHYGGPL
CCCCCCCCCCCCCCCCCCHHHCCCCCEEEECCCCCEEEEEEECCCEEEECCCHHHCCCCH
ADGLLVGDTIRCPWHHACFSLRTGEVLGPPAQDDLKCWRVEQQDGKAFVRDALPAVRPPR
HCCEEECCEECCCHHHHHEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
LASARLPESVVIIGGGAAGTAAVETLRREGYSGPVTLLSADRSLPYDRPNLSKDYLAGTA
CHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
NADWLPMRPPTFYADHDIDVRSDNRVVKLSPEQKSVTLSDGSNVSYGALLLAVGAVPIRL
CCCEECCCCCCEECCCCEEECCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCEEEEE
DVPGATLPHVSVLRTLADCDALIARLGTARRCVVVGASFIGMEVAAALRTRGLEVHVVAP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECC
EAHPMERVLGATLGDMIKALHESHGVTFHLGATVAEIQPDRVRLSTGAELAADLVVTGIG
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEECCCCEEEECCCHHHHHHHHEECCC
VRPDVALAQDAGLALDKGVAVDEFLQTSEPDIYAAGDIARWPDPGTGQRIRVEHWVVAER
CCCCCEEECCCCCEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECC
QGVVAARNILGQRQRFAAVPFFWTQHYDVAINYVGHAEQWDRFDVDGDPAAHDCTVTYWG
CCHHHHHHHHHHHHHEEECCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEC
KDKRLAVATVGRDLASLRAEVAFEQETTGH
CCCEEEEEEHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TKSSSSTDKPDLTQGVPLADLADGGMVTGQVGGKPVLLVRRADELFAIGATCTHYGGPL
CCCCCCCCCCCCCCCCCHHHCCCCCEEEECCCCCEEEEEEECCCEEEECCCHHHCCCCH
ADGLLVGDTIRCPWHHACFSLRTGEVLGPPAQDDLKCWRVEQQDGKAFVRDALPAVRPPR
HCCEEECCEECCCHHHHHEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
LASARLPESVVIIGGGAAGTAAVETLRREGYSGPVTLLSADRSLPYDRPNLSKDYLAGTA
CHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
NADWLPMRPPTFYADHDIDVRSDNRVVKLSPEQKSVTLSDGSNVSYGALLLAVGAVPIRL
CCCEECCCCCCEECCCCEEECCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCEEEEE
DVPGATLPHVSVLRTLADCDALIARLGTARRCVVVGASFIGMEVAAALRTRGLEVHVVAP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECC
EAHPMERVLGATLGDMIKALHESHGVTFHLGATVAEIQPDRVRLSTGAELAADLVVTGIG
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEECCCCEEEECCCHHHHHHHHEECCC
VRPDVALAQDAGLALDKGVAVDEFLQTSEPDIYAAGDIARWPDPGTGQRIRVEHWVVAER
CCCCCEEECCCCCEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECC
QGVVAARNILGQRQRFAAVPFFWTQHYDVAINYVGHAEQWDRFDVDGDPAAHDCTVTYWG
CCHHHHHHHHHHHHHEEECCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEC
KDKRLAVATVGRDLASLRAEVAFEQETTGH
CCCEEEEEEHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]