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The map label for this gene is siaT [H]

Identifier: 116694827

GI number: 116694827

Start: 998479

End: 999786

Strand: Direct

Name: siaT [H]

Synonym: H16_B0881

Alternate gene names: 116694827

Gene position: 998479-999786 (Clockwise)

Preceding gene: 116694826

Following gene: 116694828

Centisome position: 34.28

GC content: 66.44

Gene sequence:

>1308_bases
ATGATCGGTCCCATTGAAATCCTGCTCGGGCTGTGCCTGATGCTGGGGCTGATGTTCCTTGGCTTCCATGTGGCCACCAG
CATGCTGGTGGTGGGCGTGGCCGGGGCGCTGGCCTATTTCGGCATGCCAGCGATCGACGCATTGGGCGAACAGTTGTGGG
CAGCCGGCGAGGACTACCTGCTGCTGTCGATCCCGCTCTATATCCTGCTGGGCGAGATCCTGATGCGCGGCGGCGCGACG
GACAAGATGTACCAGTCGCTCGGCGACTGGCTGAACCGCCTGCCGGGCGGCCTGCTCCATACCAATATCGGTGCCTCGGC
CTTGTTCTCGGCGGTGTCAGGGTCATCGGTGGCCACCGCGGCGACGATTTCCACGGTCGCGCTGCCTTCCTTCCGCAATC
GCGGCTATGACCAGCGCATGGTGCTGGGCTCGATCGCGGCCGGCGCCAGCCTGGGCAACCTGATCCCGCCCGGCGTGGCC
TTCATCATCTACGGCTCGATGACCAATACTTCGGCGGCGCGGCTGTATGTGGCGGGCGTGCTCCCCGGGGCCATCATGAC
CGTGCTGTTCCTGGCCACGATCCTGCTGATCTGCCTGTGGAAGCCCGCGGCGGGCGGCCCGCGCGAGGCCCGTGTGCCGC
TGCTCGCCAGGCTGAGCAACTCGCGCCACCTGGCGGGCCCGCTGCTGGTGTTCACGGTGGTGATGGGCAGCATCTACACC
GGCTGGGCCACGGTCACCGAGAGCGCCGCGCTCGGCGTGCTGGTGTGCCTGCCGGTGGCGGCCGCCTACGGCAAGCTCAG
CGTCGCCATGCTGCACGACTGCTTCCGCTCCACGCTGCGCCTGACCGCGATGACCGCGCTGATCCTGGTGGCCGCGCTCT
ACCTGAACTTCGTGCTCTCCATGCTGGGGGTGCCGCAGGCGCTGGCCGGCCTGATGAAGCAGGTGGACGTGTCGCCGGCA
GTGTTGCTGTGGCTGCTGGCAGCCCTCTACCTGGTGCTGGGCATCGCGCTGGAGACCATGCCGATGCTGGTCGGCACGGT
CCCGGTGGTCTTCCCGATCGTGCAGGCGGCCGGCATCGATCCCGTGTTCTTCGGCGTGTTCATGGTGCTGATGTGCGAGA
TCTCGCTGATCAGCCCGCCGATCGGCATGACGCTCTACGTGATCCAGGGCGTGCGCGGCGAAGGATCGATCAATGAAGTC
TTCCAGGGCACGCTGCCGTTCTTTGCCGCCATGGTGGTGATGACCGCCGTGCTGATCCATTTCCCGGCGATTGCCACCTA
TCTGCCCAACCTGATGTTCGGGCATTGA

Upstream 100 bases:

>100_bases
AGATCGAGGCGGAGGCGGACATCCGCGTCGCCGCCGCGCCCGCTGAGGGCAATGGCGCAGGCCAGGGCAGCGCTGAAGGC
AAGAGCGAAGGAGCGGCAGC

Downstream 100 bases:

>100_bases
CCTCCAAGGAAGCTCCCATGAACGCCAAACGAATCGCCGGCGCGCTGATCGCGGCCGCCCATGTCGCCTGCGTTGCCGCC
GTGCTGCTGCCGGGCACTGC

Product: TRAP-type transporter, large permease component

Products: NA

Alternate protein names: N-acetylneuraminic acid permease; N-acetylneuraminic acid transporter; Neu5Ac permease [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIGPIEILLGLCLMLGLMFLGFHVATSMLVVGVAGALAYFGMPAIDALGEQLWAAGEDYLLLSIPLYILLGEILMRGGAT
DKMYQSLGDWLNRLPGGLLHTNIGASALFSAVSGSSVATAATISTVALPSFRNRGYDQRMVLGSIAAGASLGNLIPPGVA
FIIYGSMTNTSAARLYVAGVLPGAIMTVLFLATILLICLWKPAAGGPREARVPLLARLSNSRHLAGPLLVFTVVMGSIYT
GWATVTESAALGVLVCLPVAAAYGKLSVAMLHDCFRSTLRLTAMTALILVAALYLNFVLSMLGVPQALAGLMKQVDVSPA
VLLWLLAALYLVLGIALETMPMLVGTVPVVFPIVQAAGIDPVFFGVFMVLMCEISLISPPIGMTLYVIQGVRGEGSINEV
FQGTLPFFAAMVVMTAVLIHFPAIATYLPNLMFGH

Sequences:

>Translated_435_residues
MIGPIEILLGLCLMLGLMFLGFHVATSMLVVGVAGALAYFGMPAIDALGEQLWAAGEDYLLLSIPLYILLGEILMRGGAT
DKMYQSLGDWLNRLPGGLLHTNIGASALFSAVSGSSVATAATISTVALPSFRNRGYDQRMVLGSIAAGASLGNLIPPGVA
FIIYGSMTNTSAARLYVAGVLPGAIMTVLFLATILLICLWKPAAGGPREARVPLLARLSNSRHLAGPLLVFTVVMGSIYT
GWATVTESAALGVLVCLPVAAAYGKLSVAMLHDCFRSTLRLTAMTALILVAALYLNFVLSMLGVPQALAGLMKQVDVSPA
VLLWLLAALYLVLGIALETMPMLVGTVPVVFPIVQAAGIDPVFFGVFMVLMCEISLISPPIGMTLYVIQGVRGEGSINEV
FQGTLPFFAAMVVMTAVLIHFPAIATYLPNLMFGH
>Mature_435_residues
MIGPIEILLGLCLMLGLMFLGFHVATSMLVVGVAGALAYFGMPAIDALGEQLWAAGEDYLLLSIPLYILLGEILMRGGAT
DKMYQSLGDWLNRLPGGLLHTNIGASALFSAVSGSSVATAATISTVALPSFRNRGYDQRMVLGSIAAGASLGNLIPPGVA
FIIYGSMTNTSAARLYVAGVLPGAIMTVLFLATILLICLWKPAAGGPREARVPLLARLSNSRHLAGPLLVFTVVMGSIYT
GWATVTESAALGVLVCLPVAAAYGKLSVAMLHDCFRSTLRLTAMTALILVAALYLNFVLSMLGVPQALAGLMKQVDVSPA
VLLWLLAALYLVLGIALETMPMLVGTVPVVFPIVQAAGIDPVFFGVFMVLMCEISLISPPIGMTLYVIQGVRGEGSINEV
FQGTLPFFAAMVVMTAVLIHFPAIATYLPNLMFGH

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid [H]

COG id: COG1593

COG function: function code G; TRAP-type C4-dicarboxylate transport system, large permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the TRAP transporter large permease family [H]

Homologues:

Organism=Escherichia coli, GI48994950, Length=402, Percent_Identity=27.1144278606965, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010656
- InterPro:   IPR007387
- InterPro:   IPR004681 [H]

Pfam domain/function: PF06808 DctM; PF04290 DctQ [H]

EC number: NA

Molecular weight: Translated: 45692; Mature: 45692

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGPIEILLGLCLMLGLMFLGFHVATSMLVVGVAGALAYFGMPAIDALGEQLWAAGEDYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEE
LLSIPLYILLGEILMRGGATDKMYQSLGDWLNRLPGGLLHTNIGASALFSAVSGSSVATA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCHHHH
ATISTVALPSFRNRGYDQRMVLGSIAAGASLGNLIPPGVAFIIYGSMTNTSAARLYVAGV
HHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
LPGAIMTVLFLATILLICLWKPAAGGPREARVPLLARLSNSRHLAGPLLVFTVVMGSIYT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GWATVTESAALGVLVCLPVAAAYGKLSVAMLHDCFRSTLRLTAMTALILVAALYLNFVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGVPQALAGLMKQVDVSPAVLLWLLAALYLVLGIALETMPMLVGTVPVVFPIVQAAGID
HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVFFGVFMVLMCEISLISPPIGMTLYVIQGVRGEGSINEVFQGTLPFFAAMVVMTAVLIH
HHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPAIATYLPNLMFGH
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGPIEILLGLCLMLGLMFLGFHVATSMLVVGVAGALAYFGMPAIDALGEQLWAAGEDYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEE
LLSIPLYILLGEILMRGGATDKMYQSLGDWLNRLPGGLLHTNIGASALFSAVSGSSVATA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCHHHH
ATISTVALPSFRNRGYDQRMVLGSIAAGASLGNLIPPGVAFIIYGSMTNTSAARLYVAGV
HHHHHHHCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
LPGAIMTVLFLATILLICLWKPAAGGPREARVPLLARLSNSRHLAGPLLVFTVVMGSIYT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GWATVTESAALGVLVCLPVAAAYGKLSVAMLHDCFRSTLRLTAMTALILVAALYLNFVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGVPQALAGLMKQVDVSPAVLLWLLAALYLVLGIALETMPMLVGTVPVVFPIVQAAGID
HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVFFGVFMVLMCEISLISPPIGMTLYVIQGVRGEGSINEVFQGTLPFFAAMVVMTAVLIH
HHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPAIATYLPNLMFGH
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]