Click here to switch to the map view.

The map label for this gene is kstD [H]

Identifier: 116694590

GI number: 116694590

Start: 713272

End: 715026

Strand: Direct

Name: kstD [H]

Synonym: H16_B0639

Alternate gene names: 116694590

Gene position: 713272-715026 (Clockwise)

Preceding gene: 116694589

Following gene: 116694591

Centisome position: 24.49

GC content: 71.62

Gene sequence:

>1755_bases
ATGAGCCAGAGCAGAGCGCGCATGCAGCCCAGCGAGTTCGACGTGGTGGTGGTCGGCTCGGGCGCCGGCGGCATGCTGGC
CGCCTGCCGCGCCGCGGACCGCGGACTGTCGGTGGTGGTGCTGGAAAAGAGCAGCCAGTACGGCGGCACCTCGGCGGTGT
CCGGCGGCGGCATCTGGATTCCGCTCAACCACCATATCGCGCCGGCGGGCGGGCGCGACGACTACGCCACGGCGCTGGAA
TACATCCTGGCGTGCGCCGGCGAGCACGGCGATCCACAGCGCGTGCGCGCCTATCTTGAGCAGGCGCCCCGCATGCTCCA
GTACCTGGAGCAGCAGGCCGGCGTGCGTTACTACACGCTGCCGCGCTACGCGGACTACTTCCAGAAGGTTCCGGGCGCGA
TGCCGGGCTACCGCGCGCTCGACCCGATGCCGTTCGACGGCGCGCAGCTGCGCGAGGAATTCGCACGGCTGCGGCCGCCG
TCGCCGGGCACGCTGGTGGGCGGGCGCGTGGCCGTGACCTCGGCCGAGGCGCATGCGCTGCTGTGCCGCGCGCCCGGCTG
GCTGGGCCTGGCGGTGCGCCAGTTTGCCCGCTACTGGCTGGACCTGGGCTGGCGCCGCAAGACCCGGCGCGACCGCCGGC
TCACGCTCGGCAACAGCCTGGTCGGCGGCCTGCGGCGGGCCATGATGGACCGCGCGATTCCGCTGTGGCTCGACACCGCG
CTGCAGGACCTGATCGTCGAGGATGGCACCGTGCGCGGCGTGCGCGCGGTGCAGGACGGGCGCGTGGTGGAGATCCGCGC
GCTGCGCGGCGTGATCCTGGCCGCCGGCGGCTTCGAGCGCAACCAGGCCATGCGCGAACAGTACCTGCCGCAGCCGACCC
AGGCCGCCTGGAGCGCGACCCCGCCCAACAACACCGGTGACGGCATCCGCGCCGCCCAGGCCGCCGGCGCCGGCGTGGCG
CTGATGTCCCACGTCTGGGGCGCACCCACCGTGCATGTGCAGGGCGAGGAAAAGCAGCGCGCGCTCTTTATCGAGCGCGC
CATGCCCGGCTGCCTGGTGGTGAACGGGCAGGGCCGGCGCTTCGTCAACGAGGCCGCGCCGTATTCCGAGTTCGTTCCCG
CCATGTACCGCGACCACGAGAAAACCGGCAGCAGCGTGCCGGCGTGGATGGTGTTCGACGCCGCCTTCCGCCACAAATAT
CCGTGCGGGCCGATCCTGCCCGGCTCGGTCATGCCGGACCGCAGCATTCCGGCAAGCCTGTCCGGCATCCTGGTGCGCGC
CGACTCGCTGGCGCAGCTGGCGCAGCGGATCGGTGTCGATGCCGGCGGCCTGGCCGACAGCGTGGCGCGCATGAACCACT
ATGCTGCCACCGGCGTGGACGAGGAGTTCGGCAAGGGCGACAACCTGTTCGATACCTACTACAGCGACCCGGCAGTGCGG
CCCAACCCGTGCCTGGCGCCGATCGGCAAGGCGCCGTTCTACGCCGTGCGCATCGATGCCGGCGACATCGGCACCAAGGG
CGGCCTGGTGACGGACGCCAGCGCGCGCGTGCTGCGCGACGACGGCAGCGCCATTGCCGGGCTGTTCGCCATCGGCAATA
CCAGTGCCTCGATGATGGGCACCAGCTATCCCGGCGCGGGCTCGACACTGGGCCCGGCCATGACCTTCGGCTTTATCGCT
GCCGACGTGCTCTCTCAGAACGCGCGCCAGCCTGCCGCCGCGCCCCATGCTCAAACCGTACAGGAGGCCCTATGA

Upstream 100 bases:

>100_bases
AACAACCTGGACCGCTTCGGCCAGAACCTGGGCGGCGTGATGATGGGCCGCGCCAACACTGACTTCTTCATTTGAGCCGC
GCGCCGCACGGAGATCCAAT

Downstream 100 bases:

>100_bases
GCGCCAACCCCGAACCCAGCACCGCGCTGCGCGAAGACATGCTGATGGCGATGCGCCGCATGGCGCGCTCGGTGGCGGTG
ATCAGCTGCCGCCATGGCGA

Product: 3-oxosteroid 1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSQSRARMQPSEFDVVVVGSGAGGMLAACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWIPLNHHIAPAGGRDDYATALE
YILACAGEHGDPQRVRAYLEQAPRMLQYLEQQAGVRYYTLPRYADYFQKVPGAMPGYRALDPMPFDGAQLREEFARLRPP
SPGTLVGGRVAVTSAEAHALLCRAPGWLGLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLVGGLRRAMMDRAIPLWLDTA
LQDLIVEDGTVRGVRAVQDGRVVEIRALRGVILAAGGFERNQAMREQYLPQPTQAAWSATPPNNTGDGIRAAQAAGAGVA
LMSHVWGAPTVHVQGEEKQRALFIERAMPGCLVVNGQGRRFVNEAAPYSEFVPAMYRDHEKTGSSVPAWMVFDAAFRHKY
PCGPILPGSVMPDRSIPASLSGILVRADSLAQLAQRIGVDAGGLADSVARMNHYAATGVDEEFGKGDNLFDTYYSDPAVR
PNPCLAPIGKAPFYAVRIDAGDIGTKGGLVTDASARVLRDDGSAIAGLFAIGNTSASMMGTSYPGAGSTLGPAMTFGFIA
ADVLSQNARQPAAAPHAQTVQEAL

Sequences:

>Translated_584_residues
MSQSRARMQPSEFDVVVVGSGAGGMLAACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWIPLNHHIAPAGGRDDYATALE
YILACAGEHGDPQRVRAYLEQAPRMLQYLEQQAGVRYYTLPRYADYFQKVPGAMPGYRALDPMPFDGAQLREEFARLRPP
SPGTLVGGRVAVTSAEAHALLCRAPGWLGLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLVGGLRRAMMDRAIPLWLDTA
LQDLIVEDGTVRGVRAVQDGRVVEIRALRGVILAAGGFERNQAMREQYLPQPTQAAWSATPPNNTGDGIRAAQAAGAGVA
LMSHVWGAPTVHVQGEEKQRALFIERAMPGCLVVNGQGRRFVNEAAPYSEFVPAMYRDHEKTGSSVPAWMVFDAAFRHKY
PCGPILPGSVMPDRSIPASLSGILVRADSLAQLAQRIGVDAGGLADSVARMNHYAATGVDEEFGKGDNLFDTYYSDPAVR
PNPCLAPIGKAPFYAVRIDAGDIGTKGGLVTDASARVLRDDGSAIAGLFAIGNTSASMMGTSYPGAGSTLGPAMTFGFIA
ADVLSQNARQPAAAPHAQTVQEAL
>Mature_583_residues
SQSRARMQPSEFDVVVVGSGAGGMLAACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWIPLNHHIAPAGGRDDYATALEY
ILACAGEHGDPQRVRAYLEQAPRMLQYLEQQAGVRYYTLPRYADYFQKVPGAMPGYRALDPMPFDGAQLREEFARLRPPS
PGTLVGGRVAVTSAEAHALLCRAPGWLGLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLVGGLRRAMMDRAIPLWLDTAL
QDLIVEDGTVRGVRAVQDGRVVEIRALRGVILAAGGFERNQAMREQYLPQPTQAAWSATPPNNTGDGIRAAQAAGAGVAL
MSHVWGAPTVHVQGEEKQRALFIERAMPGCLVVNGQGRRFVNEAAPYSEFVPAMYRDHEKTGSSVPAWMVFDAAFRHKYP
CGPILPGSVMPDRSIPASLSGILVRADSLAQLAQRIGVDAGGLADSVARMNHYAATGVDEEFGKGDNLFDTYYSDPAVRP
NPCLAPIGKAPFYAVRIDAGDIGTKGGLVTDASARVLRDDGSAIAGLFAIGNTSASMMGTSYPGAGSTLGPAMTFGFIAA
DVLSQNARQPAAAPHAQTVQEAL

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 62392; Mature: 62261

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSRARMQPSEFDVVVVGSGAGGMLAACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWI
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCEEEE
PLNHHIAPAGGRDDYATALEYILACAGEHGDPQRVRAYLEQAPRMLQYLEQQAGVRYYTL
EECCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PRYADYFQKVPGAMPGYRALDPMPFDGAQLREEFARLRPPSPGTLVGGRVAVTSAEAHAL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCEEEEECCCCEEE
LCRAPGWLGLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLVGGLRRAMMDRAIPLWLDTA
EECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHH
LQDLIVEDGTVRGVRAVQDGRVVEIRALRGVILAAGGFERNQAMREQYLPQPTQAAWSAT
HHHHHHCCCCCCCEEEECCCCEEEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCC
PPNNTGDGIRAAQAAGAGVALMSHVWGAPTVHVQGEEKQRALFIERAMPGCLVVNGQGRR
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEECCCCCC
FVNEAAPYSEFVPAMYRDHEKTGSSVPAWMVFDAAFRHKYPCGPILPGSVMPDRSIPASL
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SGILVRADSLAQLAQRIGVDAGGLADSVARMNHYAATGVDEEFGKGDNLFDTYYSDPAVR
HHHEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHCCCCCCC
PNPCLAPIGKAPFYAVRIDAGDIGTKGGLVTDASARVLRDDGSAIAGLFAIGNTSASMMG
CCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCEEECCCCEEEEEEEECCCCHHHCC
TSYPGAGSTLGPAMTFGFIAADVLSQNARQPAAAPHAQTVQEAL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
SQSRARMQPSEFDVVVVGSGAGGMLAACRAADRGLSVVVLEKSSQYGGTSAVSGGGIWI
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCEEEE
PLNHHIAPAGGRDDYATALEYILACAGEHGDPQRVRAYLEQAPRMLQYLEQQAGVRYYTL
EECCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PRYADYFQKVPGAMPGYRALDPMPFDGAQLREEFARLRPPSPGTLVGGRVAVTSAEAHAL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCEEEEECCCCEEE
LCRAPGWLGLAVRQFARYWLDLGWRRKTRRDRRLTLGNSLVGGLRRAMMDRAIPLWLDTA
EECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHH
LQDLIVEDGTVRGVRAVQDGRVVEIRALRGVILAAGGFERNQAMREQYLPQPTQAAWSAT
HHHHHHCCCCCCCEEEECCCCEEEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCC
PPNNTGDGIRAAQAAGAGVALMSHVWGAPTVHVQGEEKQRALFIERAMPGCLVVNGQGRR
CCCCCCCCHHHHHHCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEECCCCCC
FVNEAAPYSEFVPAMYRDHEKTGSSVPAWMVFDAAFRHKYPCGPILPGSVMPDRSIPASL
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SGILVRADSLAQLAQRIGVDAGGLADSVARMNHYAATGVDEEFGKGDNLFDTYYSDPAVR
HHHEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHCCCCCCC
PNPCLAPIGKAPFYAVRIDAGDIGTKGGLVTDASARVLRDDGSAIAGLFAIGNTSASMMG
CCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCEEECCCCEEEEEEEECCCCHHHCC
TSYPGAGSTLGPAMTFGFIAADVLSQNARQPAAAPHAQTVQEAL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]