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The map label for this gene is bphD [H]

Identifier: 116694551

GI number: 116694551

Start: 673221

End: 674093

Strand: Direct

Name: bphD [H]

Synonym: H16_B0600

Alternate gene names: 116694551

Gene position: 673221-674093 (Clockwise)

Preceding gene: 116694542

Following gene: 116694552

Centisome position: 23.11

GC content: 66.9

Gene sequence:

>873_bases
ATGTCCGCTACGTCACAGCACACGCCTTTGTCCCCACACCCGAACCTGCCGGTCGGGCGCTTCGTCATCACGTCAACGGG
CCTGCGCCTGCACTGCCTGGACTCCGGCAGCGGCGAGCCGGTGGTCTTTATCCACGGCAGCGGGCCGGGCGCGAGCGGGC
ACAGCAACTTCCGCCACAACGCGCCGGCCTTCGCCGAGGCGGGCCTTCGCACCGTGGTGGTGGACCTGCCCGGGTATGGC
CTGTCGTCCAAGCCGGACGATGTCGAGTACACCCTGGACTTCTTTGTCGCCGCGCTGCGTGAGCAGTTGCTGGCACTGGA
GCTGCCGCGCTGCGTGCTGGTGGGCAATTCGCTTGGCGGCGCCATCGCGCTGAAGTACGCGCTGGATTTCCCGGAGCATG
TCAGCCGCCTGGTGATGATGGCGCCCGGCGGCGTGGAAGAACGGGAAACTTATTTCCAGATGGAGGGCATCCAGCGCATG
GTGTCGCTGTTTACCGGCGGCCACATGAACCCGGACACCATGCGGCAGCTGCTGCAACTGCTGGTGCACGACGCCAGCCT
GGTCACCGACGCGCTGGTCGATGAGCGCATGGCGGTGTGCCGCGAGCAGCCGCGCGAGGTGCTGGCCACCATGAAGGTGC
CCAACCTGACCGAGCGGCTGGGCGAGATCCGCTGCCCGGTGCTTGGCTTCTGGGGCACGGAAGACCAGTTCAATCCCGCC
TCGGGCGCCACCAGATTCCTGGCCGGCTGCGCCGACGCGCGCTTCGTCATGATCAACCGCTGCGGCCACTGGGTGATGGT
CGAGCACGCCGCATATTTCAACCGTGAATGTATCGGCTTCCTGGCGGATACCGCCCAGGGCGGGGCGGCATAA

Upstream 100 bases:

>100_bases
CATCGTAGGAGAAGTGGCCGTCCGGATCGTAGTCCGAATGAACGATGCCCGCGCGGGCAGCGCTGCGCAATATGCTGGGC
AAACCACGGGAGACGCCCTC

Downstream 100 bases:

>100_bases
TTGGCTCGCACGTCCACACAAGAAGACCCAGGAGACATCATGGAATACCCGTATAGCCTGTTCGACCTGCGCGGCAAGGT
TGCCGCCATCACCGGTGCCG

Product: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

Products: NA

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSATSQHTPLSPHPNLPVGRFVITSTGLRLHCLDSGSGEPVVFIHGSGPGASGHSNFRHNAPAFAEAGLRTVVVDLPGYG
LSSKPDDVEYTLDFFVAALREQLLALELPRCVLVGNSLGGAIALKYALDFPEHVSRLVMMAPGGVEERETYFQMEGIQRM
VSLFTGGHMNPDTMRQLLQLLVHDASLVTDALVDERMAVCREQPREVLATMKVPNLTERLGEIRCPVLGFWGTEDQFNPA
SGATRFLAGCADARFVMINRCGHWVMVEHAAYFNRECIGFLADTAQGGAA

Sequences:

>Translated_290_residues
MSATSQHTPLSPHPNLPVGRFVITSTGLRLHCLDSGSGEPVVFIHGSGPGASGHSNFRHNAPAFAEAGLRTVVVDLPGYG
LSSKPDDVEYTLDFFVAALREQLLALELPRCVLVGNSLGGAIALKYALDFPEHVSRLVMMAPGGVEERETYFQMEGIQRM
VSLFTGGHMNPDTMRQLLQLLVHDASLVTDALVDERMAVCREQPREVLATMKVPNLTERLGEIRCPVLGFWGTEDQFNPA
SGATRFLAGCADARFVMINRCGHWVMVEHAAYFNRECIGFLADTAQGGAA
>Mature_289_residues
SATSQHTPLSPHPNLPVGRFVITSTGLRLHCLDSGSGEPVVFIHGSGPGASGHSNFRHNAPAFAEAGLRTVVVDLPGYGL
SSKPDDVEYTLDFFVAALREQLLALELPRCVLVGNSLGGAIALKYALDFPEHVSRLVMMAPGGVEERETYFQMEGIQRMV
SLFTGGHMNPDTMRQLLQLLVHDASLVTDALVDERMAVCREQPREVLATMKVPNLTERLGEIRCPVLGFWGTEDQFNPAS
GATRFLAGCADARFVMINRCGHWVMVEHAAYFNRECIGFLADTAQGGAA

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI189027141, Length=250, Percent_Identity=25.2, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI218777840, Length=290, Percent_Identity=23.448275862069, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI13376164, Length=290, Percent_Identity=23.448275862069, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27597073, Length=127, Percent_Identity=32.2834645669291, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081721, Length=265, Percent_Identity=33.9622641509434, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.-

Molecular weight: Translated: 31480; Mature: 31349

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSQHTPLSPHPNLPVGRFVITSTGLRLHCLDSGSGEPVVFIHGSGPGASGHSNFRHN
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
APAFAEAGLRTVVVDLPGYGLSSKPDDVEYTLDFFVAALREQLLALELPRCVLVGNSLGG
CCHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
AIALKYALDFPEHVSRLVMMAPGGVEERETYFQMEGIQRMVSLFTGGHMNPDTMRQLLQL
EEEEEECCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LVHDASLVTDALVDERMAVCREQPREVLATMKVPNLTERLGEIRCPVLGFWGTEDQFNPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCC
SGATRFLAGCADARFVMINRCGHWVMVEHAAYFNRECIGFLADTAQGGAA
CHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SATSQHTPLSPHPNLPVGRFVITSTGLRLHCLDSGSGEPVVFIHGSGPGASGHSNFRHN
CCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
APAFAEAGLRTVVVDLPGYGLSSKPDDVEYTLDFFVAALREQLLALELPRCVLVGNSLGG
CCHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
AIALKYALDFPEHVSRLVMMAPGGVEERETYFQMEGIQRMVSLFTGGHMNPDTMRQLLQL
EEEEEECCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LVHDASLVTDALVDERMAVCREQPREVLATMKVPNLTERLGEIRCPVLGFWGTEDQFNPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCCCCCCC
SGATRFLAGCADARFVMINRCGHWVMVEHAAYFNRECIGFLADTAQGGAA
CHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA