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The map label for this gene is ubiE2 [H]

Identifier: 116694525

GI number: 116694525

Start: 646425

End: 647249

Strand: Direct

Name: ubiE2 [H]

Synonym: H16_B0574

Alternate gene names: 116694525

Gene position: 646425-647249 (Clockwise)

Preceding gene: 116694524

Following gene: 116694526

Centisome position: 22.19

GC content: 69.94

Gene sequence:

>825_bases
ATGGACCACACCGCAGATTCGTTTCGGGCTTTTGAGCTGGCTGGCTGGGAAGATCCCGAGGTCGTCAGCCAGTACCAGGA
GCATCTGTCGCACGTGACCCGGCAATCGGTCGAGTCCCTGCTGGACGAGGCCCATGTGACCAGCGGGCTGCGCGTGCTGG
ATGTGGCCACCGGCTCCGGCCATGTCGCGGCGGGGGCGGTGCGGCGCGGCGCAGAGTCCGTCGGCATCGATTTCTCGCTG
GCGCAGGTGCAGCTGGCGCGCAAGCTCCATCCGGATGTCCATTACGAGCAGGCCGATGCGCAGGCGCTGCCGTTCGGCGA
TGCCAGCTTCGATGCCGTGGTCAATGGCTTCGGCATGTGCCACCTGTCCGACCCCGATGCCGCGCTGGCCGAGGCCTTCC
GCGTGCTGCGCCCGGGCGGGCGCATCGCCTTCACCGTGTGGGACACGCCCGAGCGCGCGGTCGGTTTCGGCGCGGTCTAT
GCCGCGATCCGCGCCTACGGCTCGATGGAGGTCGATATCCCCGTGGGGCCGAACTTCTTCCTGTTCAGCGATCCGGGCCA
TTGCCGCACCGCGTTGCAGCAGGCCGGCTTTGTCTCGCCCACCTGCCGCAACGTGCCGCAGGTATGGCGCTTCTCCACGC
CCGACCAGCTGTTCGATGCGCTGGCCCAGGGCACCGTGCGCGCCGCCGCCACGCTGCGCGCGCAGACTCCCAAGGCCCGC
GACGAGATCCGCGCCGTGCTGCGCGGCACCGTCGCCGCCTATATGCGCGGCAGCGGCTTCGAGGTGCCGATGCCGGCAGT
GCTGGCGGCGGCGGTCAAGCCCTGA

Upstream 100 bases:

>100_bases
CCGCCAGCGCTGTCTATACTTGGTTTTACCGGGGTCAGACGGCGGAGGGGCACTGCGTCCGCGCAAACCGGCAGCCTGGC
GTCGACGCCGAGGAGAGGGC

Downstream 100 bases:

>100_bases
GGGCGGGGCTTACGCACCTGTGGCGGCGCGGACGCCGCCGAGGGTGTTGGAGATCGACCGACATCCCTGCCGCCAGCGTC
GGCCTGGCCCCACCATCCCA

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MDHTADSFRAFELAGWEDPEVVSQYQEHLSHVTRQSVESLLDEAHVTSGLRVLDVATGSGHVAAGAVRRGAESVGIDFSL
AQVQLARKLHPDVHYEQADAQALPFGDASFDAVVNGFGMCHLSDPDAALAEAFRVLRPGGRIAFTVWDTPERAVGFGAVY
AAIRAYGSMEVDIPVGPNFFLFSDPGHCRTALQQAGFVSPTCRNVPQVWRFSTPDQLFDALAQGTVRAAATLRAQTPKAR
DEIRAVLRGTVAAYMRGSGFEVPMPAVLAAAVKP

Sequences:

>Translated_274_residues
MDHTADSFRAFELAGWEDPEVVSQYQEHLSHVTRQSVESLLDEAHVTSGLRVLDVATGSGHVAAGAVRRGAESVGIDFSL
AQVQLARKLHPDVHYEQADAQALPFGDASFDAVVNGFGMCHLSDPDAALAEAFRVLRPGGRIAFTVWDTPERAVGFGAVY
AAIRAYGSMEVDIPVGPNFFLFSDPGHCRTALQQAGFVSPTCRNVPQVWRFSTPDQLFDALAQGTVRAAATLRAQTPKAR
DEIRAVLRGTVAAYMRGSGFEVPMPAVLAAAVKP
>Mature_274_residues
MDHTADSFRAFELAGWEDPEVVSQYQEHLSHVTRQSVESLLDEAHVTSGLRVLDVATGSGHVAAGAVRRGAESVGIDFSL
AQVQLARKLHPDVHYEQADAQALPFGDASFDAVVNGFGMCHLSDPDAALAEAFRVLRPGGRIAFTVWDTPERAVGFGAVY
AAIRAYGSMEVDIPVGPNFFLFSDPGHCRTALQQAGFVSPTCRNVPQVWRFSTPDQLFDALAQGTVRAAATLRAQTPKAR
DEIRAVLRGTVAAYMRGSGFEVPMPAVLAAAVKP

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=119, Percent_Identity=36.1344537815126, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29419; Mature: 29419

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHTADSFRAFELAGWEDPEVVSQYQEHLSHVTRQSVESLLDEAHVTSGLRVLDVATGSG
CCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
HVAAGAVRRGAESVGIDFSLAQVQLARKLHPDVHYEQADAQALPFGDASFDAVVNGFGMC
CHHHHHHHHCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEE
HLSDPDAALAEAFRVLRPGGRIAFTVWDTPERAVGFGAVYAAIRAYGSMEVDIPVGPNFF
EECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCEE
LFSDPGHCRTALQQAGFVSPTCRNVPQVWRFSTPDQLFDALAQGTVRAAATLRAQTPKAR
EECCCCHHHHHHHHCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIRAVLRGTVAAYMRGSGFEVPMPAVLAAAVKP
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MDHTADSFRAFELAGWEDPEVVSQYQEHLSHVTRQSVESLLDEAHVTSGLRVLDVATGSG
CCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
HVAAGAVRRGAESVGIDFSLAQVQLARKLHPDVHYEQADAQALPFGDASFDAVVNGFGMC
CHHHHHHHHCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEE
HLSDPDAALAEAFRVLRPGGRIAFTVWDTPERAVGFGAVYAAIRAYGSMEVDIPVGPNFF
EECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCEE
LFSDPGHCRTALQQAGFVSPTCRNVPQVWRFSTPDQLFDALAQGTVRAAATLRAQTPKAR
EECCCCHHHHHHHHCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIRAVLRGTVAAYMRGSGFEVPMPAVLAAAVKP
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA