Click here to switch to the map view.

The map label for this gene is mhpE1 [C]

Identifier: 116694503

GI number: 116694503

Start: 621579

End: 622643

Strand: Direct

Name: mhpE1 [C]

Synonym: H16_B0552

Alternate gene names: 116694503

Gene position: 621579-622643 (Clockwise)

Preceding gene: 116694502

Following gene: 116694505

Centisome position: 21.34

GC content: 70.14

Gene sequence:

>1065_bases
ATGACCGCACAACCCACCAAGAAGCTCTACATCTCGGACGTGACGCTGCGCGACGGCAGCCACGCCATCCGCCACCAGTA
CAGCCTGGAGCACGTGCGCCGCATCGCGGCCGCGCTCGACGCCGCGCGCGTGGATTCGATCGAGGTCGCGCACGGCGACG
GGCTGTCGGGCTCCAGCTTCAACTACGGCTTTGGCGCCCATACCGACCTGGAATGGATCGCCGCCGTGGCCGAGACCGTG
CGCCATGCCCGCGTGGCCACGCTGCTGCTGCCGGGCGTGGGCACCTTGCACGACCTGCGCGCCGCCTATGATGCCGGCGC
GCGCGTGGTGCGCGTGGCCACGCACTGCACCGAAGCCGATGTCTCGCGCCAGCACATCGAATATGCGCGCGAACTCGGCA
TGGACACCGTCGGCTTCCTGATGATGAGCCACATGACCACGCCCGCGGCGCTGGCGCAGCAGGCGAAGCTGATGGAAAGC
TACGGCGCCCAGTGCGTGTATGTGGTCGATTCCGGCGGCGCGCTGGGCATGCAGGACGTGCGCGAGCGCTTCCGCGCCTT
CAAGGACGTGCTCAGGCCGGAAACCCAGACCGGCATGCACGCCCATCACAACCTGAGCCTGGGCGTGGCCAATTCCATCG
TCGCCGTGGAAGAGGGCTGCGACCGCGTCGACGCCAGCCTGGCCGGCATGGGTGCCGGCGCCGGCAACGCCCCGCTGGAA
GTCTTTATCGCCGCCGCCGAACGCCTGGGCTGGCAGCACGGCTGCGACCTCTACACGCTGATGGATGCCGCCGACGACAT
CGTGCGCCCGCTGCAGGACCGCCCGGTGCGCGTCGACCGCGAGACCCTGGCGCTGGGCTACGCCGGGGTCTATTCAAGCT
TCCTGCGCCATGCCGAGGCCGCCGCGGCGCGCTACGGCCTGGGCACGGTCGACATCCTCGTCGAGCTGGGCCGGCGGCGC
ATGGTGGGCGGGCAGGAAGACATGATCGTGGATGTGGCGCTGGATTTGCTGGCGCGGCGCAGTGCTCAGGCGGGGCAGGC
AGTGCCGTCGGCGGCGGTGGCCTGA

Upstream 100 bases:

>100_bases
CCTATGCCGGCAACCTCGACATCATGACCAGCGCCGCACTGGCCTGCGCCGAGCGCCTGGCCGAGACCCGGCTGTCCGCA
TGAGCCGCAGGGAGACAAAG

Downstream 100 bases:

>100_bases
CCGTCGGCAGGCGCGGTCAGGGCGACGCCGGCGCGGCGCGCGCCGCAACGATCGCCAGCATCGGGCCGCTCACGCTCAAT
GGCCAGGGCCGGCTGCGCGG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 2; 4-hydroxy-2-keto-pentanoic acid aldolase 2; 4-hydroxy-2-oxopentanoate aldolase 2

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTAQPTKKLYISDVTLRDGSHAIRHQYSLEHVRRIAAALDAARVDSIEVAHGDGLSGSSFNYGFGAHTDLEWIAAVAETV
RHARVATLLLPGVGTLHDLRAAYDAGARVVRVATHCTEADVSRQHIEYARELGMDTVGFLMMSHMTTPAALAQQAKLMES
YGAQCVYVVDSGGALGMQDVRERFRAFKDVLRPETQTGMHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLE
VFIAAAERLGWQHGCDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEAAAARYGLGTVDILVELGRRR
MVGGQEDMIVDVALDLLARRSAQAGQAVPSAAVA

Sequences:

>Translated_354_residues
MTAQPTKKLYISDVTLRDGSHAIRHQYSLEHVRRIAAALDAARVDSIEVAHGDGLSGSSFNYGFGAHTDLEWIAAVAETV
RHARVATLLLPGVGTLHDLRAAYDAGARVVRVATHCTEADVSRQHIEYARELGMDTVGFLMMSHMTTPAALAQQAKLMES
YGAQCVYVVDSGGALGMQDVRERFRAFKDVLRPETQTGMHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLE
VFIAAAERLGWQHGCDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEAAAARYGLGTVDILVELGRRR
MVGGQEDMIVDVALDLLARRSAQAGQAVPSAAVA
>Mature_353_residues
TAQPTKKLYISDVTLRDGSHAIRHQYSLEHVRRIAAALDAARVDSIEVAHGDGLSGSSFNYGFGAHTDLEWIAAVAETVR
HARVATLLLPGVGTLHDLRAAYDAGARVVRVATHCTEADVSRQHIEYARELGMDTVGFLMMSHMTTPAALAQQAKLMESY
GAQCVYVVDSGGALGMQDVRERFRAFKDVLRPETQTGMHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLEV
FIAAAERLGWQHGCDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEAAAARYGLGTVDILVELGRRRM
VGGQEDMIVDVALDLLARRSAQAGQAVPSAAVA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=330, Percent_Identity=85.1515151515152, Blast_Score=565, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA2_CUPNH (Q0K3S5)

Other databases:

- EMBL:   AM260480
- RefSeq:   YP_728714.1
- ProteinModelPortal:   Q0K3S5
- SMR:   Q0K3S5
- STRING:   Q0K3S5
- GeneID:   4456317
- GenomeReviews:   AM260480_GR
- KEGG:   reh:H16_B0552
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   ATCVYVV
- ProtClustDB:   PRK08195
- BioCyc:   REUT381666:H16_B0552-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 37958; Mature: 37826

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 171-171 BINDING 200-200 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQPTKKLYISDVTLRDGSHAIRHQYSLEHVRRIAAALDAARVDSIEVAHGDGLSGSSF
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
NYGFGAHTDLEWIAAVAETVRHARVATLLLPGVGTLHDLRAAYDAGARVVRVATHCTEAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHCCHHH
VSRQHIEYARELGMDTVGFLMMSHMTTPAALAQQAKLMESYGAQCVYVVDSGGALGMQDV
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHH
RERFRAFKDVLRPETQTGMHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLE
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHH
VFIAAAERLGWQHGCDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
AAARYGLGTVDILVELGRRRMVGGQEDMIVDVALDLLARRSAQAGQAVPSAAVA
HHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure 
TAQPTKKLYISDVTLRDGSHAIRHQYSLEHVRRIAAALDAARVDSIEVAHGDGLSGSSF
CCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
NYGFGAHTDLEWIAAVAETVRHARVATLLLPGVGTLHDLRAAYDAGARVVRVATHCTEAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHCCHHH
VSRQHIEYARELGMDTVGFLMMSHMTTPAALAQQAKLMESYGAQCVYVVDSGGALGMQDV
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHH
RERFRAFKDVLRPETQTGMHAHHNLSLGVANSIVAVEEGCDRVDASLAGMGAGAGNAPLE
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHH
VFIAAAERLGWQHGCDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
AAARYGLGTVDILVELGRRRMVGGQEDMIVDVALDLLARRSAQAGQAVPSAAVA
HHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA