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The map label for this gene is mhpD1 [H]

Identifier: 116694499

GI number: 116694499

Start: 618770

End: 619552

Strand: Direct

Name: mhpD1 [H]

Synonym: H16_B0548

Alternate gene names: 116694499

Gene position: 618770-619552 (Clockwise)

Preceding gene: 116694498

Following gene: 116694500

Centisome position: 21.25

GC content: 68.2

Gene sequence:

>783_bases
ATGCATAACGAACTTATCCTTGAGATCGGCGCCGGCCTGCACCATGCGCTGGCCTCCCGTCGCCCGGTGGCGCCGCTGAC
CGAATCGTGGCCGGAGCTGAGCATCGACGATGCCTACCGCATCCAGCTGGCGATGGTGCAGCATCGGCTCGACGCCGGCG
AGCGCGTGGTCGGCAAGAAGATCGGTGTGACCAGCCGCGTGGTGATGGACATGCTCGACGTGCGCCAGCCCGACTTTGGC
CACCTGCTGTCCGGCATGGTCTACCCCGACGGCGCGGCGATTCCCGCCGACACCCTGATCGCGCCCAAGGCCGAAGGCGA
GATTGCCTTCGTGCTGAAGGAAGACCTGGAGGGGCCGGGCGTGACCAATGCCGACGTGCTGCGCGCCACCGCCTATGTGC
TGCCTTGCTTCGAGATCGTCGACTCGCGCATCCGCGACTGGAAGATCCGCATCCAGGACACCGTGGCTGACAACGCATCG
TCAGGCGTGTTCGTGCTGGGCGATGCCGCGGTGGACCCGCGCAGCGTGGACCTGGGCACGGTCGGCATGACGCTGGAGAA
AAACGGCGAGATCGTCGCGACCGGCGCCGGCGCGGCCGCGCTCGGGCATCCCGTCAATGCGGTGGCGTGGCTGGCCAATA
CGCTTGGCCGGCTGGGCATTGGCCTGAAGCGGGGCGAGGTGATCCTGTCCGGCTCTCTTGCCGCGATGGTGCCCGTAACC
GCCGGCGACCAGCTGCGCATCAGCCTGGGCGGCATCGGCTCGGCCGGCGTGCGCTTCGTCTGA

Upstream 100 bases:

>100_bases
CGGCGCCAGGCAGTCCGGCATCGGCCGCGAGGGCGGTGTCCATTCGCTCGAGTTCTACACCGAGCTGCGCAACGTCTGCG
TCCGCCTGTGAGGTCCCGTC

Downstream 100 bases:

>100_bases
ACCCGACCCATTTTTCAATTCAGCGCAGGACATGCCATGAATCTGACACACGACACCATTGCCCGGCTCGCCGAGCACCT
GGAGAACGCCGAGCTGCAGC

Product: 2-oxopent-4-enoate hydratase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MHNELILEIGAGLHHALASRRPVAPLTESWPELSIDDAYRIQLAMVQHRLDAGERVVGKKIGVTSRVVMDMLDVRQPDFG
HLLSGMVYPDGAAIPADTLIAPKAEGEIAFVLKEDLEGPGVTNADVLRATAYVLPCFEIVDSRIRDWKIRIQDTVADNAS
SGVFVLGDAAVDPRSVDLGTVGMTLEKNGEIVATGAGAAALGHPVNAVAWLANTLGRLGIGLKRGEVILSGSLAAMVPVT
AGDQLRISLGGIGSAGVRFV

Sequences:

>Translated_260_residues
MHNELILEIGAGLHHALASRRPVAPLTESWPELSIDDAYRIQLAMVQHRLDAGERVVGKKIGVTSRVVMDMLDVRQPDFG
HLLSGMVYPDGAAIPADTLIAPKAEGEIAFVLKEDLEGPGVTNADVLRATAYVLPCFEIVDSRIRDWKIRIQDTVADNAS
SGVFVLGDAAVDPRSVDLGTVGMTLEKNGEIVATGAGAAALGHPVNAVAWLANTLGRLGIGLKRGEVILSGSLAAMVPVT
AGDQLRISLGGIGSAGVRFV
>Mature_260_residues
MHNELILEIGAGLHHALASRRPVAPLTESWPELSIDDAYRIQLAMVQHRLDAGERVVGKKIGVTSRVVMDMLDVRQPDFG
HLLSGMVYPDGAAIPADTLIAPKAEGEIAFVLKEDLEGPGVTNADVLRATAYVLPCFEIVDSRIRDWKIRIQDTVADNAS
SGVFVLGDAAVDPRSVDLGTVGMTLEKNGEIVATGAGAAALGHPVNAVAWLANTLGRLGIGLKRGEVILSGSLAAMVPVT
AGDQLRISLGGIGSAGVRFV

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=259, Percent_Identity=42.0849420849421, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 27325; Mature: 27325

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNELILEIGAGLHHALASRRPVAPLTESWPELSIDDAYRIQLAMVQHRLDAGERVVGKK
CCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
IGVTSRVVMDMLDVRQPDFGHLLSGMVYPDGAAIPADTLIAPKAEGEIAFVLKEDLEGPG
CCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEECCCCCCCEEEEEEECCCCCC
VTNADVLRATAYVLPCFEIVDSRIRDWKIRIQDTVADNASSGVFVLGDAAVDPRSVDLGT
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEECCCCCCCCCCEEE
VGMTLEKNGEIVATGAGAAALGHPVNAVAWLANTLGRLGIGLKRGEVILSGSLAAMVPVT
EEEEEECCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEEC
AGDQLRISLGGIGSAGVRFV
CCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MHNELILEIGAGLHHALASRRPVAPLTESWPELSIDDAYRIQLAMVQHRLDAGERVVGKK
CCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
IGVTSRVVMDMLDVRQPDFGHLLSGMVYPDGAAIPADTLIAPKAEGEIAFVLKEDLEGPG
CCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEECCCCCCCEEEEEEECCCCCC
VTNADVLRATAYVLPCFEIVDSRIRDWKIRIQDTVADNASSGVFVLGDAAVDPRSVDLGT
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEECCCCCCCCCCEEE
VGMTLEKNGEIVATGAGAAALGHPVNAVAWLANTLGRLGIGLKRGEVILSGSLAAMVPVT
EEEEEECCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEEC
AGDQLRISLGGIGSAGVRFV
CCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]