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The map label for this gene is braD [H]

Identifier: 116694454

GI number: 116694454

Start: 567726

End: 568610

Strand: Direct

Name: braD [H]

Synonym: H16_B0503

Alternate gene names: 116694454

Gene position: 567726-568610 (Clockwise)

Preceding gene: 116694453

Following gene: 116694455

Centisome position: 19.49

GC content: 64.97

Gene sequence:

>885_bases
ATGAATCTGTTTGAAATCCCGCTCCCGGCGCTGCTGTCGCAGTTGTTGCTCGGGCTCGTCAACGGCGCCTTCTACGCCAT
GCTGAGCCTGGGGCTGGCGGTCATCTTCGGGCTGCTGAATGTCATCAACTTTGCGCACGGCGCGCTCTTCATGCTGGGTG
CGGTGCTGGCGTGGGCCGGCATGGCCTATGCCGGCCTCAGCTACTGGGTGATGCTGCTGTTGTCGCCGCTGGTGGTTGCC
GCGCTGGGCGTGGGGATCGAGAAGACCATGCTGCGCTGGATCTACAAGCTCGACCATATCTACGGCCTGCTGCTGACGCT
AGGCATCACGCTGGTGATCGAAGGCATCTTCCGCTCGGTCTATGGCGTGTCCGGGCTGCCCTATGCACCGCCCGAGGCGC
TGCAGGGCGCCTCCGACCTCGGCTTCATGCGCTTGCCCAACTACCGCGTGTGGGTGGTCGGGGCATCGCTGGCGGTCTGC
CTGTCGACGTGGTACGTGATCGAGAAGACCAGGCTGGGCGCCTACCTGCGCGCCGGCACCGAGAACGCCAAGGTGGTCGA
GGCGTTCGGCGTCAACGTGCCGCGGATGGTGACGCTGACCTATGGCTTCGGCGTGGCGCTGGCCGCGTTTGCCGGGGTGC
TGGCGGCGCCCGTCATGCAGGTATCGCCGCTGATGGGGCAGAACCTGATCATCGTCGTGTTCGCGGTGGTGGTGATCGGC
GGCATGGGCTCGATCATGGGCGCGATCCTGACCGGCCTGGGGCTGGGCGTGATCGAGGGGCTGGCCAAGGTGTTGTACCC
GGAAGCATCTTCCACCGTTGTCTTCGTCATCATGGTGGTGGTGCTGTTGTTGCGCCCGGCAGGGCTGTTCGGAAAGGAGA
AGTGA

Upstream 100 bases:

>100_bases
GTGATGGAGCACGGCAGCATCGTCGAGCGCTTCGCGGCCAATGAGCTGGAGGCCAGGATGCCGGTGCTGCATGAACTGCT
GGGCGTATAAGGAGCGACTC

Downstream 100 bases:

>100_bases
TGAGCGGACAATCTATCGCCGCAATCGGCATGCGGCAGCAGCAGGCGGCGGGGCGCCTGCCAAAGCAGGTGATGTACGGC
GTGGTGCTGGCGTGCCTGCT

Product: ABC-type transporter, permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNLFEIPLPALLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWAGMAYAGLSYWVMLLLSPLVVA
ALGVGIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSVYGVSGLPYAPPEALQGASDLGFMRLPNYRVWVVGASLAVC
LSTWYVIEKTRLGAYLRAGTENAKVVEAFGVNVPRMVTLTYGFGVALAAFAGVLAAPVMQVSPLMGQNLIIVVFAVVVIG
GMGSIMGAILTGLGLGVIEGLAKVLYPEASSTVVFVIMVVVLLLRPAGLFGKEK

Sequences:

>Translated_294_residues
MNLFEIPLPALLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWAGMAYAGLSYWVMLLLSPLVVA
ALGVGIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSVYGVSGLPYAPPEALQGASDLGFMRLPNYRVWVVGASLAVC
LSTWYVIEKTRLGAYLRAGTENAKVVEAFGVNVPRMVTLTYGFGVALAAFAGVLAAPVMQVSPLMGQNLIIVVFAVVVIG
GMGSIMGAILTGLGLGVIEGLAKVLYPEASSTVVFVIMVVVLLLRPAGLFGKEK
>Mature_294_residues
MNLFEIPLPALLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWAGMAYAGLSYWVMLLLSPLVVA
ALGVGIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSVYGVSGLPYAPPEALQGASDLGFMRLPNYRVWVVGASLAVC
LSTWYVIEKTRLGAYLRAGTENAKVVEAFGVNVPRMVTLTYGFGVALAAFAGVLAAPVMQVSPLMGQNLIIVVFAVVVIG
GMGSIMGAILTGLGLGVIEGLAKVLYPEASSTVVFVIMVVVLLLRPAGLFGKEK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=319, Percent_Identity=26.6457680250784, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31110; Mature: 31110

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFEIPLPALLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAYAGLSYWVMLLLSPLVVAALGVGIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVSGLPYAPPEALQGASDLGFMRLPNYRVWVVGASLAVCLSTWYVIEKTRLGAYLRAGT
HCCCCCCCCCHHHHCCCCCCCCEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENAKVVEAFGVNVPRMVTLTYGFGVALAAFAGVLAAPVMQVSPLMGQNLIIVVFAVVVIG
CCCHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GMGSIMGAILTGLGLGVIEGLAKVLYPEASSTVVFVIMVVVLLLRPAGLFGKEK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNLFEIPLPALLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAYAGLSYWVMLLLSPLVVAALGVGIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVSGLPYAPPEALQGASDLGFMRLPNYRVWVVGASLAVCLSTWYVIEKTRLGAYLRAGT
HCCCCCCCCCHHHHCCCCCCCCEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENAKVVEAFGVNVPRMVTLTYGFGVALAAFAGVLAAPVMQVSPLMGQNLIIVVFAVVVIG
CCCHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GMGSIMGAILTGLGLGVIEGLAKVLYPEASSTVVFVIMVVVLLLRPAGLFGKEK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]