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The map label for this gene is fliA [H]

Identifier: 116694211

GI number: 116694211

Start: 294367

End: 295083

Strand: Direct

Name: fliA [H]

Synonym: H16_B0256

Alternate gene names: 116694211

Gene position: 294367-295083 (Clockwise)

Preceding gene: 116694209

Following gene: 116694212

Centisome position: 10.11

GC content: 66.95

Gene sequence:

>717_bases
ATGTACACGATCCAGGGAAAGCTCGAACAGGCCGATGTGGTCAAGGCGCATGCACAGCTGGTGCGCCGCATCGCCCTGCA
ACTGGCCGCCAGGCTGCCTGCCAGCGTGCAGATCGACGACCTGATCCAGGCCGGCATGATCGGCCTGCTGGATGCCGCCA
AGCGTTATGAAGACACCCACGGCGCGCGCTTCGAGACCTACGCCAGCCAGCGCATCCGCGGCGCCATGCTTGACGAGGTG
CGCGCCAACGACTGGCAGTCGCGCAGCCTGCGCCAGTTCACCCGGCGCATCGAGCGCACGCAGCGCGGGCTGGAGCAGAA
GCTCGGGCGCGCGCCGCTCGACTCCGAAGTGGCCGAGGCCATGGAGATGGCGCTGGACGAATACCAGCTGCTGCTCAATG
AGGTCTATGGCTGCCAGCTGCTGCACTACGAGGATTTCGAGCGCTCCGGCGAAGAAGACTTCCTGGACCGGCACCTTGGC
GGCAGCGATGACGGCAACCCGCTGACCGTGCTGATGGAAAGCGGCATGCGCGAGGCGCTGATCCGCGCCATCGACCGGCT
GCCGGAGCGCGAGAAGCTGGTGCTGTCGCTGTGCTATGACCAGGAGCTGAACCTGCGCGAGATCGGCGCGGTGCTCGATG
TGACCGAGTCGCGCGTGTGCCAGATCCGCGGCCAGGCGATCACGCGGCTGCGCAACCAGCTGCGCGGCTTGCTGTGA

Upstream 100 bases:

>100_bases
TGAGCCTGCCAGCCATGCCATCCAACCGAACCAGACCCTGAGCCAGCCCCCGCCGCACGTTGCGGCGGTCCAGCAATCCA
GCATTCACGAGAATTGCACC

Downstream 100 bases:

>100_bases
TGCAGGCGCTGGGCCGCGCCGCGCGCGGCGCTTGCTGGCTGCTGGCTGCCGCATCGGCCTGCGCCTGGGCCGCCATCGAG
GGCGGCACCCGCCACGCCTG

Product: DNA-directed RNA polymerase sigma subunit / flagellum specific

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MYTIQGKLEQADVVKAHAQLVRRIALQLAARLPASVQIDDLIQAGMIGLLDAAKRYEDTHGARFETYASQRIRGAMLDEV
RANDWQSRSLRQFTRRIERTQRGLEQKLGRAPLDSEVAEAMEMALDEYQLLLNEVYGCQLLHYEDFERSGEEDFLDRHLG
GSDDGNPLTVLMESGMREALIRAIDRLPEREKLVLSLCYDQELNLREIGAVLDVTESRVCQIRGQAITRLRNQLRGLL

Sequences:

>Translated_238_residues
MYTIQGKLEQADVVKAHAQLVRRIALQLAARLPASVQIDDLIQAGMIGLLDAAKRYEDTHGARFETYASQRIRGAMLDEV
RANDWQSRSLRQFTRRIERTQRGLEQKLGRAPLDSEVAEAMEMALDEYQLLLNEVYGCQLLHYEDFERSGEEDFLDRHLG
GSDDGNPLTVLMESGMREALIRAIDRLPEREKLVLSLCYDQELNLREIGAVLDVTESRVCQIRGQAITRLRNQLRGLL
>Mature_238_residues
MYTIQGKLEQADVVKAHAQLVRRIALQLAARLPASVQIDDLIQAGMIGLLDAAKRYEDTHGARFETYASQRIRGAMLDEV
RANDWQSRSLRQFTRRIERTQRGLEQKLGRAPLDSEVAEAMEMALDEYQLLLNEVYGCQLLHYEDFERSGEEDFLDRHLG
GSDDGNPLTVLMESGMREALIRAIDRLPEREKLVLSLCYDQELNLREIGAVLDVTESRVCQIRGQAITRLRNQLRGLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellar operon [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=234, Percent_Identity=46.5811965811966, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=30.2904564315353, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27168; Mature: 27168

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTIQGKLEQADVVKAHAQLVRRIALQLAARLPASVQIDDLIQAGMIGLLDAAKRYEDTH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GARFETYASQRIRGAMLDEVRANDWQSRSLRQFTRRIERTQRGLEQKLGRAPLDSEVAEA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
MEMALDEYQLLLNEVYGCQLLHYEDFERSGEEDFLDRHLGGSDDGNPLTVLMESGMREAL
HHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IRAIDRLPEREKLVLSLCYDQELNLREIGAVLDVTESRVCQIRGQAITRLRNQLRGLL
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTIQGKLEQADVVKAHAQLVRRIALQLAARLPASVQIDDLIQAGMIGLLDAAKRYEDTH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GARFETYASQRIRGAMLDEVRANDWQSRSLRQFTRRIERTQRGLEQKLGRAPLDSEVAEA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
MEMALDEYQLLLNEVYGCQLLHYEDFERSGEEDFLDRHLGGSDDGNPLTVLMESGMREAL
HHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IRAIDRLPEREKLVLSLCYDQELNLREIGAVLDVTESRVCQIRGQAITRLRNQLRGLL
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730257 [H]