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The map label for this gene is rhmD [H]

Identifier: 116694172

GI number: 116694172

Start: 244676

End: 245851

Strand: Direct

Name: rhmD [H]

Synonym: H16_B0217

Alternate gene names: 116694172

Gene position: 244676-245851 (Clockwise)

Preceding gene: 116694171

Following gene: 116694174

Centisome position: 8.4

GC content: 64.37

Gene sequence:

>1176_bases
ATGAAGATCACCAACGTCCGCGCCCGTGTCTTTGAATGGAAGGGCAAGACCGTGCCGCCGCAGGCGCATTTCTGCACCAA
CGCCACCGACATCCTGTTCGAGCGCGGCGACGCCATGGGCTCGTTCCGCTTCCACGGCTGGCTGGTGGTTGAAGTGGAAA
CCGATTCCGGCCTGGTCGGCATCGGCAACTGCGCGCTGGCGCCGCGCGTGGCCAAGGAGATCGTCGACCAGTACCTGGCG
CCGGCGGTGATCGGCCAGGACCCGTTCGACAACGAATACCTGTGGCAGAAGATGTACCGCCGCACCCATGCCTGGGGCCG
CAAGGGCATCGGCATGGCGGCGATCTCGGCGGTCGATATCGCGCTGTGGGACCTGATGGGCAAGGCGGTTGGGAAGCCCG
TGTTCAAGCTGCTGGGCGGGCGCACCAAGGAAAAAATCTGGTGCTACGCCTCCAAGCTCTACAACAATGACGACCGCGAC
GCGTTCCTGGCAGAAGCGCAGCGCTACCTCGACCAGGGCTTCACCGCGATGAAGATGCGCTTCGGCTACGGCCCCAAGGA
CGGCCCCGCCGGCATGCAGAAGAACATCGAACAGGTGCGCCTGCTGCGCGAGCTGGTCGGCGACGGCGTCGACATCATGC
TGGAGTGCTACATGGGCTGGACCCTGGAGTACGCGCGCCGCATGCTGCCGCGCCTGGCCGAGTTCAACCCGCGCTGGCTG
GAAGAGCCGGTGATCGCCGACGATATCGAGGGCTATGCCGAGCTGAAGAAGGCGAGCCCGTTCCCGATCTCCGGCGGTGA
ACATGAATTCACCTCCTACGGCTTCAAGGACCTGCTCGAGCGCCGTGCGGTGGACGTGATCCAGTACGACACCAACCGCG
TCGGCGGCATCACCGCGGCGCAGAAGATCAACGCCATGGCCGAGGCCTGGTCGGTGCCGGTGATCCCGCACGCGGGGCAG
ATGCATAACTACCACCTGACCATGGCCTCGACGGCTTCGCCGATGTCGGAGTACTTCCCCGTGCACGACGTCGAGGTGGG
CAATGAGTTGTTCTACTACATCTTCAAGGGCGATCCGGCGCCGGACAACGGCTACCTGCAGCTCGACGACAACCTGCCGG
GGCTGGGCCTGGAGCTGAACGAGGAGTACTTCAGCCAGTTCGACATCATCGGCTGA

Upstream 100 bases:

>100_bases
CACAGCCTAGCCACCACCTTCACCACACCAAATCCAGCAAGACCGCCCCCGGCGCGGCCGGGGAGCGTTCGCGCCAACGC
ATCGCCATCAGGAGCTGAAC

Downstream 100 bases:

>100_bases
CGGGCCAGTTGCCGGGTTCTGCCCGGCCGGGCAGCGCGCCATGTTTCACGACAATGCGCGCTGCCGCTTCAAGGCCGCCT
CTTCTTCCACTCATAGTCCG

Product: L-alanine-DL-glutamate epimerase/enolase superfamily-like protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKITNVRARVFEWKGKTVPPQAHFCTNATDILFERGDAMGSFRFHGWLVVEVETDSGLVGIGNCALAPRVAKEIVDQYLA
PAVIGQDPFDNEYLWQKMYRRTHAWGRKGIGMAAISAVDIALWDLMGKAVGKPVFKLLGGRTKEKIWCYASKLYNNDDRD
AFLAEAQRYLDQGFTAMKMRFGYGPKDGPAGMQKNIEQVRLLRELVGDGVDIMLECYMGWTLEYARRMLPRLAEFNPRWL
EEPVIADDIEGYAELKKASPFPISGGEHEFTSYGFKDLLERRAVDVIQYDTNRVGGITAAQKINAMAEAWSVPVIPHAGQ
MHNYHLTMASTASPMSEYFPVHDVEVGNELFYYIFKGDPAPDNGYLQLDDNLPGLGLELNEEYFSQFDIIG

Sequences:

>Translated_391_residues
MKITNVRARVFEWKGKTVPPQAHFCTNATDILFERGDAMGSFRFHGWLVVEVETDSGLVGIGNCALAPRVAKEIVDQYLA
PAVIGQDPFDNEYLWQKMYRRTHAWGRKGIGMAAISAVDIALWDLMGKAVGKPVFKLLGGRTKEKIWCYASKLYNNDDRD
AFLAEAQRYLDQGFTAMKMRFGYGPKDGPAGMQKNIEQVRLLRELVGDGVDIMLECYMGWTLEYARRMLPRLAEFNPRWL
EEPVIADDIEGYAELKKASPFPISGGEHEFTSYGFKDLLERRAVDVIQYDTNRVGGITAAQKINAMAEAWSVPVIPHAGQ
MHNYHLTMASTASPMSEYFPVHDVEVGNELFYYIFKGDPAPDNGYLQLDDNLPGLGLELNEEYFSQFDIIG
>Mature_391_residues
MKITNVRARVFEWKGKTVPPQAHFCTNATDILFERGDAMGSFRFHGWLVVEVETDSGLVGIGNCALAPRVAKEIVDQYLA
PAVIGQDPFDNEYLWQKMYRRTHAWGRKGIGMAAISAVDIALWDLMGKAVGKPVFKLLGGRTKEKIWCYASKLYNNDDRD
AFLAEAQRYLDQGFTAMKMRFGYGPKDGPAGMQKNIEQVRLLRELVGDGVDIMLECYMGWTLEYARRMLPRLAEFNPRWL
EEPVIADDIEGYAELKKASPFPISGGEHEFTSYGFKDLLERRAVDVIQYDTNRVGGITAAQKINAMAEAWSVPVIPHAGQ
MHNYHLTMASTASPMSEYFPVHDVEVGNELFYYIFKGDPAPDNGYLQLDDNLPGLGLELNEEYFSQFDIIG

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=321, Percent_Identity=26.791277258567, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI186972148, Length=264, Percent_Identity=28.7878787878788, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI42544117, Length=318, Percent_Identity=26.1006289308176, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI226510960, Length=344, Percent_Identity=33.4302325581395, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI48994953, Length=350, Percent_Identity=29.7142857142857, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787864, Length=373, Percent_Identity=25.4691689008043, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44096; Mature: 44096

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITNVRARVFEWKGKTVPPQAHFCTNATDILFERGDAMGSFRFHGWLVVEVETDSGLVG
CCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHCCCCCCCCEEEEEEEEEEEECCCCEEE
IGNCALAPRVAKEIVDQYLAPAVIGQDPFDNEYLWQKMYRRTHAWGRKGIGMAAISAVDI
CCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ALWDLMGKAVGKPVFKLLGGRTKEKIWCYASKLYNNDDRDAFLAEAQRYLDQGFTAMKMR
HHHHHHHHHHCHHHHHHHCCCCCHHHEEEHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
FGYGPKDGPAGMQKNIEQVRLLRELVGDGVDIMLECYMGWTLEYARRMLPRLAEFNPRWL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EEPVIADDIEGYAELKKASPFPISGGEHEFTSYGFKDLLERRAVDVIQYDTNRVGGITAA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHEEECCCCCCCHHHH
QKINAMAEAWSVPVIPHAGQMHNYHLTMASTASPMSEYFPVHDVEVGNELFYYIFKGDPA
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCEEECCCEEEEEEEECCCC
PDNGYLQLDDNLPGLGLELNEEYFSQFDIIG
CCCCEEEECCCCCCCCCEECHHHHHHCCCCC
>Mature Secondary Structure
MKITNVRARVFEWKGKTVPPQAHFCTNATDILFERGDAMGSFRFHGWLVVEVETDSGLVG
CCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHCCCCCCCCEEEEEEEEEEEECCCCEEE
IGNCALAPRVAKEIVDQYLAPAVIGQDPFDNEYLWQKMYRRTHAWGRKGIGMAAISAVDI
CCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ALWDLMGKAVGKPVFKLLGGRTKEKIWCYASKLYNNDDRDAFLAEAQRYLDQGFTAMKMR
HHHHHHHHHHCHHHHHHHCCCCCHHHEEEHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
FGYGPKDGPAGMQKNIEQVRLLRELVGDGVDIMLECYMGWTLEYARRMLPRLAEFNPRWL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EEPVIADDIEGYAELKKASPFPISGGEHEFTSYGFKDLLERRAVDVIQYDTNRVGGITAA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHEEECCCCCCCHHHH
QKINAMAEAWSVPVIPHAGQMHNYHLTMASTASPMSEYFPVHDVEVGNELFYYIFKGDPA
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCEEECCCEEEEEEEECCCC
PDNGYLQLDDNLPGLGLELNEEYFSQFDIIG
CCCCEEEECCCCCCCCCEECHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA