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The map label for this gene is pcaF [H]

Identifier: 116694155

GI number: 116694155

Start: 225876

End: 227078

Strand: Direct

Name: pcaF [H]

Synonym: H16_B0200

Alternate gene names: 116694155

Gene position: 225876-227078 (Clockwise)

Preceding gene: 116694154

Following gene: 116694156

Centisome position: 7.76

GC content: 69.58

Gene sequence:

>1203_bases
ATGACCGAAGCCTTTATCTGCGACGCCATCCGCACCCCCATCGGCCGCTACGGCGGCAGCCTGTCCGCGGTGCGCCCGGA
CGACCTCGGCGCGGTGCCGCTGAAGGCGCTGATGGCCCGCAATCCGAACCTGGACTGGAAGGCCATCGATGACGTGATCT
ACGGCAACGCCAACCAGGCCGGCGAAGACAACCGCAACGTGGCACGCATGTCGGCGCTGCTGGCGGGCCTGCCGCAAGAG
GTGCCGGGCGCCACCATCAACCGCCTGTGCGGCTCCGGCATGGACGCTACCGGCACCGCCGCGCGCGCCATCAAGGCCGG
CGAAGCCACCCTGATGATCGCCGGCGGCGTGGAAAGCATGAGCCGTGCCCCGTTCGTGATGGGCAAGGCCACCAGCGCGT
TCTCGCGCGATGCGCAGATCTTCGACACCACCATCGGCTGGCGCTTCATCAACCCGGCCATGCGCGCGGCCTACGGCGTG
GACTCGATGCCCGAGACCGCCGAGAACGTCGCCACCGACTACAAGATCAGCCGCGAAGACCAGGACCTGATGGCGCTGCG
CAGCCAGGAAAAGGCCTCGCGCGCGCAGGCCGACGGCACGCTGGCGCAGGAAATAACCCCGGTCACCATCGCCCAGAAGA
AGGGCGACCCGATCGTGGTCGAGCGCGACGAGCACCCGCGCGCCACCAGCATGGAAGCGCTGGCCAAGCTGCGCGGCGTG
GTCCGTCCTGACGGCACCGTGACCGCTGGCAATGCCTCGGGCGTGAACGACGGCGCCTGCGCGCTGCTGCTGGCCAGCGA
AGCCGGCCTCAAGCAGCACGGCCTGACCCCGCGCGCCCGCATCGTCGGCATGGCCACCGCCGGCGTGGCGCCGCGCGTGA
TGGGCATCGGCCCGGCACCGGCGTCGCAGAAGCTGCTCAGGCAGCTGGGCATGACCATGGACCAGATCGACGTGATCGAG
CTGAACGAAGCGTTCGCCGCGCAAGGCCTGGCCGTGCTGCGTGAACTGGGCGTGGCCGACGACGACAAGCGCGTCAACCC
GAACGGCGGCGCGATCGCGCTGGGCCACCCGCTGGGCATGAGCGGCGCGCGCCTGGTGACCACCGCGATGTACCAGCTGC
ATCGCACCGGTGGGCACTTTGCGCTGTGCACGATGTGCATTGGCGTGGGCCAGGGTATTGCGATGGTGATCGAGCGGGTC
TAA

Upstream 100 bases:

>100_bases
GGGCTGAGCTTCGATGAACTGCAGCGCATTACCGGCGTTCCCCTGAAGCTGGCCGCCTGAGCGCCGGCATCACCACACAC
CAAAACGAGAGAGACACACC

Downstream 100 bases:

>100_bases
GTCAAGCCAGTTGGAAAGCTGAGGGTGTACTCCCTCTCCCGCAAGCGGGAGAGGGAGCGAGTTAGCGGTAGTTCTTGGAG
TGAGCAAGAGATGTCCCTTC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFICDAIRTPIGRYGGSLSAVRPDDLGAVPLKALMARNPNLDWKAIDDVIYGNANQAGEDNRNVARMSALLAGLPQE
VPGATINRLCGSGMDATGTAARAIKAGEATLMIAGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGV
DSMPETAENVATDYKISREDQDLMALRSQEKASRAQADGTLAQEITPVTIAQKKGDPIVVERDEHPRATSMEALAKLRGV
VRPDGTVTAGNASGVNDGACALLLASEAGLKQHGLTPRARIVGMATAGVAPRVMGIGPAPASQKLLRQLGMTMDQIDVIE
LNEAFAAQGLAVLRELGVADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTGGHFALCTMCIGVGQGIAMVIERV

Sequences:

>Translated_400_residues
MTEAFICDAIRTPIGRYGGSLSAVRPDDLGAVPLKALMARNPNLDWKAIDDVIYGNANQAGEDNRNVARMSALLAGLPQE
VPGATINRLCGSGMDATGTAARAIKAGEATLMIAGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGV
DSMPETAENVATDYKISREDQDLMALRSQEKASRAQADGTLAQEITPVTIAQKKGDPIVVERDEHPRATSMEALAKLRGV
VRPDGTVTAGNASGVNDGACALLLASEAGLKQHGLTPRARIVGMATAGVAPRVMGIGPAPASQKLLRQLGMTMDQIDVIE
LNEAFAAQGLAVLRELGVADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTGGHFALCTMCIGVGQGIAMVIERV
>Mature_399_residues
TEAFICDAIRTPIGRYGGSLSAVRPDDLGAVPLKALMARNPNLDWKAIDDVIYGNANQAGEDNRNVARMSALLAGLPQEV
PGATINRLCGSGMDATGTAARAIKAGEATLMIAGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVD
SMPETAENVATDYKISREDQDLMALRSQEKASRAQADGTLAQEITPVTIAQKKGDPIVVERDEHPRATSMEALAKLRGVV
RPDGTVTAGNASGVNDGACALLLASEAGLKQHGLTPRARIVGMATAGVAPRVMGIGPAPASQKLLRQLGMTMDQIDVIEL
NEAFAAQGLAVLRELGVADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTGGHFALCTMCIGVGQGIAMVIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=42.0654911838791, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=39.4472361809045, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=35.1620947630923, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=39.5989974937343, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.615925058548, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=29.9492385786802, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.0798004987531, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=46.8982630272953, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=43.7037037037037, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.8927680798005, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=31.8396226415094, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=43.3915211970075, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=35.427135678392, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=34.7290640394089, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=30.787037037037, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.8765432098765, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=39, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=35.6608478802993, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.8902743142145, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=40.6947890818859, Blast_Score=267, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=34.320987654321, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.7830188679245, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41890; Mature: 41758

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFICDAIRTPIGRYGGSLSAVRPDDLGAVPLKALMARNPNLDWKAIDDVIYGNANQA
CCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
GEDNRNVARMSALLAGLPQEVPGATINRLCGSGMDATGTAARAIKAGEATLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCHHHH
SRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVDSMPETAENVATDYKISRED
CCCCEEEECCHHHHCCCCHHEEHHCCCEECCHHHHHHHCCCCCCHHHHHHHHCCEECCCC
QDLMALRSQEKASRAQADGTLAQEITPVTIAQKKGDPIVVERDEHPRATSMEALAKLRGV
HHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLLASEAGLKQHGLTPRARIVGMATAGVAPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECCCCHHHCCCCCHHHEEEEECCCCCCCEEECCCCC
ASQKLLRQLGMTMDQIDVIELNEAFAAQGLAVLRELGVADDDKRVNPNGGAIALGHPLGM
HHHHHHHHHCCCHHCEEEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTAMYQLHRTGGHFALCTMCIGVGQGIAMVIERV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TEAFICDAIRTPIGRYGGSLSAVRPDDLGAVPLKALMARNPNLDWKAIDDVIYGNANQA
CCHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
GEDNRNVARMSALLAGLPQEVPGATINRLCGSGMDATGTAARAIKAGEATLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCHHHH
SRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVDSMPETAENVATDYKISRED
CCCCEEEECCHHHHCCCCHHEEHHCCCEECCHHHHHHHCCCCCCHHHHHHHHCCEECCCC
QDLMALRSQEKASRAQADGTLAQEITPVTIAQKKGDPIVVERDEHPRATSMEALAKLRGV
HHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLLASEAGLKQHGLTPRARIVGMATAGVAPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECCCCHHHCCCCCHHHEEEEECCCCCCCEEECCCCC
ASQKLLRQLGMTMDQIDVIELNEAFAAQGLAVLRELGVADDDKRVNPNGGAIALGHPLGM
HHHHHHHHHCCCHHCEEEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTAMYQLHRTGGHFALCTMCIGVGQGIAMVIERV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]