Click here to switch to the map view.

The map label for this gene is pcaJ [H]

Identifier: 116694154

GI number: 116694154

Start: 225194

End: 225835

Strand: Direct

Name: pcaJ [H]

Synonym: H16_B0199

Alternate gene names: 116694154

Gene position: 225194-225835 (Clockwise)

Preceding gene: 116694153

Following gene: 116694155

Centisome position: 7.73

GC content: 66.98

Gene sequence:

>642_bases
ATGCAACGCCTGACCCGCGATCAGATGGCCGCCCGCGTGGCCAAAGACATTCCCGACGGTGCCGTGGTCAACCTCGGCAT
CGGCCTGCCGACACTGGTCGGCAACCACCTGCCCGCAGACAAGGAAATCCTGCTGCACAGCGAGAACGGCCTGCTTGGCA
TGGGCCCCGCGCCCGCGCCCGGCGAGGAAGACGGCGACCTGATCAATGCCGGCAAGCAGCCGGTGACGGTCAAGCCGGGC
GGCTCGTTCTTCCATCACGCCGATTCGTTCGCGATGATGCGTGGCGGCCACCTGGACTTCTGCGTGCTGGGTGCGTTCCA
GGTCTCAGAGAAGGGCGACCTGGCCAACTGGCACACCGGCGCGCCCGGCGCCATCCCCGCCGTGGGCGGCGCGATGGACC
TGGCCATCGGCGCCAAGCAGGTGTTCGTGATGATGGAGCACCAGACCAAGCAGGGCGAGAGCAAGATTGTGCCGCAGTGC
ACCTATCCGCTGACCGGCATCGGCTGCGTGACCCGCATCTACACTGACCTTGCGACCATCGACGTGACCGCCGACGGGCT
GGTCGCGCGCGACCTGGTCGAAGGGCTGAGCTTCGATGAACTGCAGCGCATTACCGGCGTTCCCCTGAAGCTGGCCGCCT
GA

Upstream 100 bases:

>100_bases
GGAGCTGGGCGAGATGGACCCCGAACACATCGTGACGCCGGGCCTCTTCGTCAAGCGCGTCGTCAAGGTCGCCTGAGCGA
CAGGTAACAGGAGAATCCAG

Downstream 100 bases:

>100_bases
GCGCCGGCATCACCACACACCAAAACGAGAGAGACACACCATGACCGAAGCCTTTATCTGCGACGCCATCCGCACCCCCA
TCGGCCGCTACGGCGGCAGC

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAPGEEDGDLINAGKQPVTVKPG
GSFFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHQTKQGESKIVPQC
TYPLTGIGCVTRIYTDLATIDVTADGLVARDLVEGLSFDELQRITGVPLKLAA

Sequences:

>Translated_213_residues
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAPGEEDGDLINAGKQPVTVKPG
GSFFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHQTKQGESKIVPQC
TYPLTGIGCVTRIYTDLATIDVTADGLVARDLVEGLSFDELQRITGVPLKLAA
>Mature_213_residues
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAPGEEDGDLINAGKQPVTVKPG
GSFFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHQTKQGESKIVPQC
TYPLTGIGCVTRIYTDLATIDVTADGLVARDLVEGLSFDELQRITGVPLKLAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=210, Percent_Identity=48.5714285714286, Blast_Score=188, Evalue=3e-48,
Organism=Homo sapiens, GI11545841, Length=210, Percent_Identity=49.047619047619, Blast_Score=187, Evalue=4e-48,
Organism=Escherichia coli, GI1788552, Length=209, Percent_Identity=43.5406698564593, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17531517, Length=212, Percent_Identity=47.1698113207547, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21358325, Length=213, Percent_Identity=44.131455399061, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655794, Length=213, Percent_Identity=44.131455399061, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22332; Mature: 22332

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAP
CCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCEEEEECCCCEEECCCCCCC
GEEDGDLINAGKQPVTVKPGGSFFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTG
CCCCCCEECCCCCCEEECCCCCCHHCCCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
APGAIPAVGGAMDLAIGAKQVFVMMEHQTKQGESKIVPQCTYPLTGIGCVTRIYTDLATI
CCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
DVTADGLVARDLVEGLSFDELQRITGVPLKLAA
EECCCCHHHHHHHHCCCHHHHHHHCCCCEEECC
>Mature Secondary Structure
MQRLTRDQMAARVAKDIPDGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAP
CCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCEEEEECCCCEEECCCCCCC
GEEDGDLINAGKQPVTVKPGGSFFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTG
CCCCCCEECCCCCCEEECCCCCCHHCCCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
APGAIPAVGGAMDLAIGAKQVFVMMEHQTKQGESKIVPQCTYPLTGIGCVTRIYTDLATI
CCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
DVTADGLVARDLVEGLSFDELQRITGVPLKLAA
EECCCCHHHHHHHHCCCHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]