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The map label for this gene is acoB

Identifier: 116694105

GI number: 116694105

Start: 169147

End: 170163

Strand: Direct

Name: acoB

Synonym: H16_B0145

Alternate gene names: 116694105

Gene position: 169147-170163 (Clockwise)

Preceding gene: 116694104

Following gene: 116694106

Centisome position: 5.81

GC content: 66.67

Gene sequence:

>1017_bases
ATGGCTCGCAAACTGAGCATCAAGCTGGCGATCAACGAGGCGATCGACCAGGAAATGACCCGCGACCCCAGCGTCATCAT
GCTGGGCGAAGATATCGTCGGCGGCGCCGGCGCGGACGGCGAGAAGGACGCCTGGGGCGGCGTGCTGGGCGTGACCAAGG
GTCTTTACGCCAAGCACGGCGACCGGCTGCTGGACACGCCGCTGTCTGAATCCGCTTACGTGGGCGCCGCCATCGGCGCC
GCGGCCTGCGGCATGCGCCCGATCGCCGAGCTGATGTTTATCGATTTCATGGGCGTTTGCTTCGACCAGATCTTCAACCA
GGCGGCCAAGTTCCGCTACATGTTCGGCGGCAAGGCGGAGACGCCGGTGGTGATCCGCGCGATGGTCGGCGCGGGCTTTC
GCGCAGCCGCGCAGCACAGCCAGATGCTGACGCCGCTGTTCACGCATATCCCCGGCCTGAAGGTGGTGTGCCCGTCCACG
CCGTACGACACCAAGGGCCTGCTGATCCAGGCGATCCGCGACAACGACCCGGTGATCTTCTGCGAGCACAAGAACCTGTA
CGGCCTGGAAGGCGAGGTGCCCGAAGGCGCCTACGCGATCCCGTTCGGCGAGGCCAATATCGTGCGCGACGGCAAGGACG
TGTCGATCGTCACCTACGGGTTGATGGTGCATCGCGCGCTCGAAGCGGCGGCAACGCTGGCCAAGGAGGGCATCGAGGCC
GAGATCGTGGACCTGCGCACGCTCTCGCCACTGGACATGGACACGGTGCTGGAGTCGGTCGAGAACACCGGCCGCCTGGT
GGTGGTGGACGAGGCCAGCCCGCGCTGCAATATCGCCACCGATATCTCCGCGCAGGTTGCGCAGCAAGCCTTCGGCGCGC
TCAAGGCCGGCATCGAGATGGTGTGCCCGCCGCACACGCCGGTGCCGTTCTCGCCGACGCTGGAGGACCTGTACATCCCC
AGCGCCGCGCAGATCGCGGCCGCCGCGCGCAAGACCATGAAGGGAGGCAAGCACTGA

Upstream 100 bases:

>100_bases
AGCCAAGGCCGCGCCGCAGCCGGGTCCCGAAGACCTGCTCACCGACGTCTACGTCAGCTACTGATCTGCCACGCGCTAAC
CCAATATCAGGAGACAAACC

Downstream 100 bases:

>100_bases
TGGCAACCGAAATTTCCCCCACGATTATCCCGATCGTGATGCCCAAGTGGGGCCTGTCGATGAAGGAAGGCACGGTCAAT
GCGTGGCTGGTCGACGAAGG

Product: acetoin dehydrogenase E1 component beta-subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGA
AACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPST
PYDTKGLLIQAIRDNDPVIFCEHKNLYGLEGEVPEGAYAIPFGEANIVRDGKDVSIVTYGLMVHRALEAAATLAKEGIEA
EIVDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIP
SAAQIAAAARKTMKGGKH

Sequences:

>Translated_338_residues
MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGA
AACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPST
PYDTKGLLIQAIRDNDPVIFCEHKNLYGLEGEVPEGAYAIPFGEANIVRDGKDVSIVTYGLMVHRALEAAATLAKEGIEA
EIVDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIP
SAAQIAAAARKTMKGGKH
>Mature_337_residues
ARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGAA
ACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTP
YDTKGLLIQAIRDNDPVIFCEHKNLYGLEGEVPEGAYAIPFGEANIVRDGKDVSIVTYGLMVHRALEAAATLAKEGIEAE
IVDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIPS
AAQIAAAARKTMKGGKH

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=339, Percent_Identity=39.5280235988201, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI291084858, Length=339, Percent_Identity=37.7581120943953, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI4557353, Length=337, Percent_Identity=38.5756676557863, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI34101272, Length=337, Percent_Identity=38.5756676557863, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17538422, Length=339, Percent_Identity=39.2330383480826, Blast_Score=246, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI17506935, Length=336, Percent_Identity=40.4761904761905, Blast_Score=204, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319698, Length=338, Percent_Identity=36.6863905325444, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI21358145, Length=342, Percent_Identity=37.719298245614, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24650940, Length=342, Percent_Identity=37.719298245614, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI160714832, Length=331, Percent_Identity=34.7432024169184, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI160714828, Length=331, Percent_Identity=34.7432024169184, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24650943, Length=103, Percent_Identity=34.9514563106796, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650945, Length=103, Percent_Identity=34.9514563106796, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACOB_CUPNH (P27746)

Other databases:

- EMBL:   M66060
- EMBL:   AM260480
- PIR:   C42462
- RefSeq:   YP_728316.1
- ProteinModelPortal:   P27746
- SMR:   P27746
- STRING:   P27746
- GeneID:   4454950
- GenomeReviews:   AM260480_GR
- KEGG:   reh:H16_B0145
- eggNOG:   COG0022
- HOGENOM:   HBG753264
- OMA:   CNIATDI
- ProtClustDB:   CLSK934278
- BioCyc:   REUT381666:H16_B0145-MONOMER
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476
- Gene3D:   G3DSA:3.40.50.920
- SMART:   SM00861

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; SSF52922 Transketo_C_like

EC number: 1.2.4.1

Molecular weight: Translated: 35960; Mature: 35829

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHG
CCCEEEEEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
DRLLDTPLSESAYVGAAIGAAACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAE
CHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTPYDTKGLLIQAIRDNDPVIF
CCEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEEE
CEHKNLYGLEGEVPEGAYAIPFGEANIVRDGKDVSIVTYGLMVHRALEAAATLAKEGIEA
EECCCEECCCCCCCCCCEEECCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCE
EIVDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEM
EEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCEE
VCPPHTPVPFSPTLEDLYIPSAAQIAAAARKTMKGGKH
ECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAWGGVLGVTKGLYAKHG
CCEEEEEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
DRLLDTPLSESAYVGAAIGAAACGMRPIAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAE
CHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTPYDTKGLLIQAIRDNDPVIF
CCEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEEE
CEHKNLYGLEGEVPEGAYAIPFGEANIVRDGKDVSIVTYGLMVHRALEAAATLAKEGIEA
EECCCEECCCCCCCCCCEEECCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCE
EIVDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATDISAQVAQQAFGALKAGIEM
EEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCEE
VCPPHTPVPFSPTLEDLYIPSAAQIAAAARKTMKGGKH
ECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286