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The map label for this gene is acoA

Identifier: 116694104

GI number: 116694104

Start: 168109

End: 169110

Strand: Direct

Name: acoA

Synonym: H16_B0144

Alternate gene names: 116694104

Gene position: 168109-169110 (Clockwise)

Preceding gene: 116694103

Following gene: 116694105

Centisome position: 5.77

GC content: 66.17

Gene sequence:

>1002_bases
ATGACAGCCAGAGCTTCTCAAGATTCCGCCGCGCTGCCGCTCGACAAGGAGACGTTGCTGACGGTGTACCGGAAGATGCG
CACCATCCGCGATTTCGAAGAACGCCTGCACGTGGACTTCGGGCGCGGCGACATCCCGGGCTTCGTCCATCTATACGCGG
GCGAGGAAGCCGCGGGCGTCGGCATTCTGCACCACCTGAACGATGGCGACCGCATCGCCAGCACACACCGCGGCCACGGC
CATTGCATTGCCAAGGGCGTCGATCCGGTGGCGATGATGAAGGAGATCTACGGCAAGAAGGGCGGCTCGTGCAATGGCAA
GGGTGGCTCGATGCATATCGCCGACCTGTCCAAGGGCATGATGGGCGCCAACGGCATCCTGGGCGCAGGCGCGCCGCTGA
TCTGCGGCGCCGCGCTGGCTGCCAAGTTCCGCGGCAAGGGCGAGGTCGGCATCACCTTCTGCGGCGACGGCGCCTCCAAC
CAGGGCACTTTCCTGGAAAGCCTGAACCTGGCCGCGGTCTGGAACCTGCCGGTGATCTTCGTGATCGAGAACAACGGCTA
TGCCGAATCGACCTCGCGCGATTACGGCACCGCGGTGGACAGCTACGTGGACCGCGCCGCCGGCTTCGGCATCCCGGGCG
TGACGGTGGACGGCACCGATTTCTTCGCCGTCCATGAAGCGGCCGGCGAAGTGATCCGGCGCGCGCGCGAAGGCGGCGGG
CCGTCGCTGCTCGAATGCAAGATGGTCCGCTTCTATGGCCACTTCGAGGGCGATGCGCAGACCTACCGCGCCGCCGGCGA
ACTCGACGACATCCGCGCCAACAAGGATTGCCTGAAGCTGTTTGGCCGCGCCGTGACCCAGGCGGGAGTGGTCGCTCGCG
AAGAACTCGACACCATCGACCGCGAGGTCGCCGCGCTGATCGAGCATGCCGTGCAGGAAGCCAAGGCCGCGCCGCAGCCG
GGTCCCGAAGACCTGCTCACCGACGTCTACGTCAGCTACTGA

Upstream 100 bases:

>100_bases
GCCTGCCGCAGCCCGCCGCGGTGGGATGACGCGCGGGCACCGCGTCCGCCCGCTTTTCCCCTTTCTTGAAGTGCAACACC
TGAAAACGAAGGAGACAGAC

Downstream 100 bases:

>100_bases
TCTGCCACGCGCTAACCCAATATCAGGAGACAAACCATGGCTCGCAAACTGAGCATCAAGCTGGCGATCAACGAGGCGAT
CGACCAGGAAATGACCCGCG

Product: acetoin dehydrogenase E1 component alpha-subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTARASQDSAALPLDKETLLTVYRKMRTIRDFEERLHVDFGRGDIPGFVHLYAGEEAAGVGILHHLNDGDRIASTHRGHG
HCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFCGDGASN
QGTFLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGGG
PSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFGRAVTQAGVVAREELDTIDREVAALIEHAVQEAKAAPQP
GPEDLLTDVYVSY

Sequences:

>Translated_333_residues
MTARASQDSAALPLDKETLLTVYRKMRTIRDFEERLHVDFGRGDIPGFVHLYAGEEAAGVGILHHLNDGDRIASTHRGHG
HCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFCGDGASN
QGTFLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGGG
PSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFGRAVTQAGVVAREELDTIDREVAALIEHAVQEAKAAPQP
GPEDLLTDVYVSY
>Mature_332_residues
TARASQDSAALPLDKETLLTVYRKMRTIRDFEERLHVDFGRGDIPGFVHLYAGEEAAGVGILHHLNDGDRIASTHRGHGH
CIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFCGDGASNQ
GTFLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGGGP
SLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFGRAVTQAGVVAREELDTIDREVAALIEHAVQEAKAAPQPG
PEDLLTDVYVSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=314, Percent_Identity=37.5796178343949, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=39.6875, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=39.6875, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=38.8379204892966, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=34.375, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI11386135, Length=318, Percent_Identity=27.6729559748428, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI258645172, Length=318, Percent_Identity=27.6729559748428, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536047, Length=323, Percent_Identity=36.2229102167183, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI32564172, Length=323, Percent_Identity=36.2229102167183, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI86563355, Length=319, Percent_Identity=26.3322884012539, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI86563357, Length=319, Percent_Identity=26.3322884012539, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=36.0501567398119, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24639740, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24639744, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28571106, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=37.5816993464052, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24639748, Length=320, Percent_Identity=34.375, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI21355903, Length=312, Percent_Identity=26.6025641025641, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACOA_CUPNH (P27745)

Other databases:

- EMBL:   M66060
- EMBL:   AM260480
- PIR:   B42462
- RefSeq:   YP_728315.1
- ProteinModelPortal:   P27745
- SMR:   P27745
- STRING:   P27745
- GeneID:   4454949
- GenomeReviews:   AM260480_GR
- KEGG:   reh:H16_B0144
- eggNOG:   COG1071
- HOGENOM:   HBG753263
- OMA:   NGYGEAT
- PhylomeDB:   P27745
- ProtClustDB:   CLSK934279
- BioCyc:   REUT381666:H16_B0144-MONOMER
- InterPro:   IPR001017

Pfam domain/function: PF00676 E1_dh

EC number: 1.2.4.1

Molecular weight: Translated: 35375; Mature: 35244

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARASQDSAALPLDKETLLTVYRKMRTIRDFEERLHVDFGRGDIPGFVHLYAGEEAAGV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCC
GILHHLNDGDRIASTHRGHGHCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGM
EEEEECCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCC
MGANGILGAGAPLICGAALAAKFRGKGEVGITFCGDGASNQGTFLESLNLAAVWNLPVIF
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCEEEEECCCEEE
VIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGGG
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCC
PSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFGRAVTQAGVVAREELDTID
CCHHHHEEEHHHCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
REVAALIEHAVQEAKAAPQPGPEDLLTDVYVSY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TARASQDSAALPLDKETLLTVYRKMRTIRDFEERLHVDFGRGDIPGFVHLYAGEEAAGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCC
GILHHLNDGDRIASTHRGHGHCIAKGVDPVAMMKEIYGKKGGSCNGKGGSMHIADLSKGM
EEEEECCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCC
MGANGILGAGAPLICGAALAAKFRGKGEVGITFCGDGASNQGTFLESLNLAAVWNLPVIF
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCEEEEECCCEEE
VIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFFAVHEAAGEVIRRAREGGG
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCC
PSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLFGRAVTQAGVVAREELDTID
CCHHHHEEEHHHCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
REVAALIEHAVQEAKAAPQPGPEDLLTDVYVSY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286