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The map label for this gene is miaE [H]

Identifier: 113478269

GI number: 113478269

Start: 7532687

End: 7533286

Strand: Reverse

Name: miaE [H]

Synonym: Tery_4943

Alternate gene names: 113478269

Gene position: 7533286-7532687 (Counterclockwise)

Preceding gene: 113478270

Following gene: 113478268

Centisome position: 97.2

GC content: 32.83

Gene sequence:

>600_bases
ATGCAACCATCAAAAAAAAGAACAATTAAGTATATTAAACAACCTACTTCTCAAATTTGGATAGACCAAGCGATCGCTAA
TTTAGATATTATATTATTAGATCATTCCCACTGTGAACGAAAGGCAGCCGGAGTAGCTTTAAATTTAATGTTTCGTTATC
CTTCTAGTGCTAAACTTATCAAAATGTTAACAGCTATTGCCCGTGAAGAACTAGAACATTTTGAAAAAGTAAACCAGTGG
TTAGAACGTCGTAATATTCCATTAGCTCCCTTAAATGCTTCTCCTTATGGAGCAGCTTTAAATAGTCAAGTTCGGCGGAA
TGAGCCAGAAAGAATGTTAGATTTATTATTAGTTTATTGTTTAATTGAAGCTCGTAGTCACGAACGTTTAGGCTTACTTG
CTGATTATTGCAATGACTCTGAATTAGCAAAATTTTATAGTAGTTTAATGGCATCTGAAGCTAGACATTATGGAGTCTAT
TGGATTTTAGCTACAACTTATTTTAAACAGAAAACTGTAGAGAAAAGATTAGAGGAGTTAGCAATATTTGAAAGTGAGAT
ACTAAGTAATTTACATCCAGAACCACGAATTCATAGTTAA

Upstream 100 bases:

>100_bases
AAAATGATTTTAAAAAAGTATTAGAACATTATCCAGATTTTGCAGCTCATGTTAAAAAAATAGCTAAAGAACGTTATAAA
CTTGAAAATAAAGAATAACT

Downstream 100 bases:

>100_bases
TAAATTAAGTCTTCAGGGATTAGCAATTAGCATTTAGTATTCAGGAGCCTAAATGAATTAGTAATTTTTTTGAAACTAGG
AATATTTTCATTCTGAATTA

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MQPSKKRTIKYIKQPTSQIWIDQAIANLDIILLDHSHCERKAAGVALNLMFRYPSSAKLIKMLTAIAREELEHFEKVNQW
LERRNIPLAPLNASPYGAALNSQVRRNEPERMLDLLLVYCLIEARSHERLGLLADYCNDSELAKFYSSLMASEARHYGVY
WILATTYFKQKTVEKRLEELAIFESEILSNLHPEPRIHS

Sequences:

>Translated_199_residues
MQPSKKRTIKYIKQPTSQIWIDQAIANLDIILLDHSHCERKAAGVALNLMFRYPSSAKLIKMLTAIAREELEHFEKVNQW
LERRNIPLAPLNASPYGAALNSQVRRNEPERMLDLLLVYCLIEARSHERLGLLADYCNDSELAKFYSSLMASEARHYGVY
WILATTYFKQKTVEKRLEELAIFESEILSNLHPEPRIHS
>Mature_199_residues
MQPSKKRTIKYIKQPTSQIWIDQAIANLDIILLDHSHCERKAAGVALNLMFRYPSSAKLIKMLTAIAREELEHFEKVNQW
LERRNIPLAPLNASPYGAALNSQVRRNEPERMLDLLLVYCLIEARSHERLGLLADYCNDSELAKFYSSLMASEARHYGVY
WILATTYFKQKTVEKRLEELAIFESEILSNLHPEPRIHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23083; Mature: 23083

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSKKRTIKYIKQPTSQIWIDQAIANLDIILLDHSHCERKAAGVALNLMFRYPSSAKLI
CCCCHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHEEEEEECCCHHHHH
KMLTAIAREELEHFEKVNQWLERRNIPLAPLNASPYGAALNSQVRRNEPERMLDLLLVYC
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
LIEARSHERLGLLADYCNDSELAKFYSSLMASEARHYGVYWILATTYFKQKTVEKRLEEL
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AIFESEILSNLHPEPRIHS
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQPSKKRTIKYIKQPTSQIWIDQAIANLDIILLDHSHCERKAAGVALNLMFRYPSSAKLI
CCCCHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHEEEEEECCCHHHHH
KMLTAIAREELEHFEKVNQWLERRNIPLAPLNASPYGAALNSQVRRNEPERMLDLLLVYC
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
LIEARSHERLGLLADYCNDSELAKFYSSLMASEARHYGVYWILATTYFKQKTVEKRLEEL
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AIFESEILSNLHPEPRIHS
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]