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The map label for this gene is ispE

Identifier: 113478075

GI number: 113478075

Start: 7222153

End: 7223097

Strand: Reverse

Name: ispE

Synonym: Tery_4700

Alternate gene names: 113478075

Gene position: 7223097-7222153 (Counterclockwise)

Preceding gene: 113478076

Following gene: 113478074

Centisome position: 93.2

GC content: 40.53

Gene sequence:

>945_bases
ATGCGTTCCTACTCATTAATTGCTCCTGGCAAAATTAATCTGTATCTGGAAATTATCGGCGATCGCCCAGACGGTTATCA
CGAATTAGCAATGGTCTTGCAAAGCATTAGTCTAGCAGACAAAATACATATTCGTTCGATTGGCATTGAGACTATTGTAG
TGCGATGCGACCATCCTTTAGTGCCTCAAGATGTAGACAATATTGCTTACCGAGCAGCTTCCCTGATGAGCAAAGAATTT
CCAGAAGTATTTGCTCGATACGGAGGTGTTGAAATTACTATCAATAAGTATATTCCTATGGCAGCCGGGTTAGCAGGAGG
TTCGACAAATGCAGCGGCAGTATTAGTTGGTCTAGACTTAATGTGGGAACTGGGTTTAACTCACAGGGAATTACAAGAGT
TGGGAGCACGGTTGGGTTCAGACGTTCCTTTCTGTATTGGTGGGGGAACTGCATTGGGAACGAGGCGAGGTGAAATACTT
TCTCCACTGCCAAATTTAGACCATATTTATGTGGTTTTAGCTAAATACAAAAATCTCTCTATTTCTACACCTTGGGCATA
TAAAACTTATCGTCAAAAGTTTGGCCATACTTATATTAGCGATACAGAAAGTCAGAAAAGTAGTAGGCAAAGAGTACATT
CTGGACCGATGGTATCGGCGATCGTTGAGCACAACTATAAGGAAGTTGGTGAACTATTGCATAATGATCTAGAAAAGGTG
GCTTTGTCAGAATATCCCCAATTATTAAAATTAAGGGAGGCTTTTGCCTCGGAAAATGTATTAGGAACTATGATGTCTGG
TTCAGGACCAACTATGTTTGCTTTAACAGAATCTCAATCTCAAGCAGAAGAAGTGAGGGCAGCGGTAAAAGAGAAAATGG
CTGACCCAGATTTAGAATTTTGGATAGCACAGTTTAATTCTAGTGGTATTAGTATTGCACATTAA

Upstream 100 bases:

>100_bases
TTACTGCTGTAATATTACTCTAATTTCTCTTTTTTGTACAACAAAGCTCCATACTAATATAAATTAAAATAATAAAACTA
TCTAAACTAAACCACTTAAA

Downstream 100 bases:

>100_bases
TATTAGGAGTCATTTGAAGAAATAAGCTCTTTCAGTTATTAGTTATTAGTACTAAGGGAACTCAGCAAAGAATTGAAATA
TGTATGGCATTTTATTCATC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRSYSLIAPGKINLYLEIIGDRPDGYHELAMVLQSISLADKIHIRSIGIETIVVRCDHPLVPQDVDNIAYRAASLMSKEF
PEVFARYGGVEITINKYIPMAAGLAGGSTNAAAVLVGLDLMWELGLTHRELQELGARLGSDVPFCIGGGTALGTRRGEIL
SPLPNLDHIYVVLAKYKNLSISTPWAYKTYRQKFGHTYISDTESQKSSRQRVHSGPMVSAIVEHNYKEVGELLHNDLEKV
ALSEYPQLLKLREAFASENVLGTMMSGSGPTMFALTESQSQAEEVRAAVKEKMADPDLEFWIAQFNSSGISIAH

Sequences:

>Translated_314_residues
MRSYSLIAPGKINLYLEIIGDRPDGYHELAMVLQSISLADKIHIRSIGIETIVVRCDHPLVPQDVDNIAYRAASLMSKEF
PEVFARYGGVEITINKYIPMAAGLAGGSTNAAAVLVGLDLMWELGLTHRELQELGARLGSDVPFCIGGGTALGTRRGEIL
SPLPNLDHIYVVLAKYKNLSISTPWAYKTYRQKFGHTYISDTESQKSSRQRVHSGPMVSAIVEHNYKEVGELLHNDLEKV
ALSEYPQLLKLREAFASENVLGTMMSGSGPTMFALTESQSQAEEVRAAVKEKMADPDLEFWIAQFNSSGISIAH
>Mature_314_residues
MRSYSLIAPGKINLYLEIIGDRPDGYHELAMVLQSISLADKIHIRSIGIETIVVRCDHPLVPQDVDNIAYRAASLMSKEF
PEVFARYGGVEITINKYIPMAAGLAGGSTNAAAVLVGLDLMWELGLTHRELQELGARLGSDVPFCIGGGTALGTRRGEIL
SPLPNLDHIYVVLAKYKNLSISTPWAYKTYRQKFGHTYISDTESQKSSRQRVHSGPMVSAIVEHNYKEVGELLHNDLEKV
ALSEYPQLLKLREAFASENVLGTMMSGSGPTMFALTESQSQAEEVRAAVKEKMADPDLEFWIAQFNSSGISIAH

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=317, Percent_Identity=29.6529968454259, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_TRIEI (Q10VR1)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_724136.1
- ProteinModelPortal:   Q10VR1
- STRING:   Q10VR1
- GeneID:   4246354
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_4700
- NMPDR:   fig|203124.1.peg.4441
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- PhylomeDB:   Q10VR1
- ProtClustDB:   PRK02534
- BioCyc:   TERY203124:TERY_4700-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34552; Mature: 34552

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYSLIAPGKINLYLEIIGDRPDGYHELAMVLQSISLADKIHIRSIGIETIVVRCDHPL
CCCCCEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEECEEEEEEECCCCC
VPQDVDNIAYRAASLMSKEFPEVFARYGGVEITINKYIPMAAGLAGGSTNAAAVLVGLDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCCCCEEHHHHHHH
MWELGLTHRELQELGARLGSDVPFCIGGGTALGTRRGEILSPLPNLDHIYVVLAKYKNLS
HHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHCCCCCCCCEEEEEEHHCCCC
ISTPWAYKTYRQKFGHTYISDTESQKSSRQRVHSGPMVSAIVEHNYKEVGELLHNDLEKV
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHH
ALSEYPQLLKLREAFASENVLGTMMSGSGPTMFALTESQSQAEEVRAAVKEKMADPDLEF
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHH
WIAQFNSSGISIAH
HHEEECCCCCEECC
>Mature Secondary Structure
MRSYSLIAPGKINLYLEIIGDRPDGYHELAMVLQSISLADKIHIRSIGIETIVVRCDHPL
CCCCCEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEECEEEEEEECCCCC
VPQDVDNIAYRAASLMSKEFPEVFARYGGVEITINKYIPMAAGLAGGSTNAAAVLVGLDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCCCCEEHHHHHHH
MWELGLTHRELQELGARLGSDVPFCIGGGTALGTRRGEILSPLPNLDHIYVVLAKYKNLS
HHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHCCCCCCCCEEEEEEHHCCCC
ISTPWAYKTYRQKFGHTYISDTESQKSSRQRVHSGPMVSAIVEHNYKEVGELLHNDLEKV
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHH
ALSEYPQLLKLREAFASENVLGTMMSGSGPTMFALTESQSQAEEVRAAVKEKMADPDLEF
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHH
WIAQFNSSGISIAH
HHEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA