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The map label for this gene is nspVR [H]

Identifier: 113478033

GI number: 113478033

Start: 7153055

End: 7153297

Strand: Reverse

Name: nspVR [H]

Synonym: Tery_4651

Alternate gene names: 113478033

Gene position: 7153297-7153055 (Counterclockwise)

Preceding gene: 113478034

Following gene: 113478032

Centisome position: 92.3

GC content: 31.28

Gene sequence:

>243_bases
ATGAAAGAACTTACTCTATTATTATTTAAAACGGAGACCATAACTTTTGTGAGAGATTTAAGTGTTACTATAATTTTTGA
TTTGTATGGTATTACTGATGGTAAAGCAGTGGGAACTAACTATTTAGGAAAGAAGTATTTATATACTCCAGGCAGTGCAG
CTAGTGGCATAGATTTTCCGGAATTAGAAGTAGATTTAAAGGTAAAATATATAGGACAACCACAATCATCTTGTCCGTTT
TAA

Upstream 100 bases:

>100_bases
AGAATGGATATGTAAAAGAATTGGCAGACAAAACTTTAGATTTAAGCCTCAAACTTGGCAACAGTTAACGTTATTTAGTT
AATTCAATATTTATCAATAT

Downstream 100 bases:

>100_bases
GATGCTAGTCAAAAAGTTTATGGGTTAGGTTATAATTTACTTATTTTTGTATCTAAAAAAATAGATAATTGCGAATTACA
GGCAGGAAGTTTTAATATTC

Product: hypothetical protein

Products: NA

Alternate protein names: R.NspV; Endonuclease NspV; Type II restriction enzyme NspV [H]

Number of amino acids: Translated: 80; Mature: 80

Protein sequence:

>80_residues
MKELTLLLFKTETITFVRDLSVTIIFDLYGITDGKAVGTNYLGKKYLYTPGSAASGIDFPELEVDLKVKYIGQPQSSCPF

Sequences:

>Translated_80_residues
MKELTLLLFKTETITFVRDLSVTIIFDLYGITDGKAVGTNYLGKKYLYTPGSAASGIDFPELEVDLKVKYIGQPQSSCPF
>Mature_80_residues
MKELTLLLFKTETITFVRDLSVTIIFDLYGITDGKAVGTNYLGKKYLYTPGSAASGIDFPELEVDLKVKYIGQPQSSCPF

Specific function: Recognizes the double-stranded sequence TTCGAA and cleaves after T-2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 8871; Mature: 8871

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELTLLLFKTETITFVRDLSVTIIFDLYGITDGKAVGTNYLGKKYLYTPGSAASGIDFP
CCCEEEEEEECCEEEEEECCEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ELEVDLKVKYIGQPQSSCPF
CEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKELTLLLFKTETITFVRDLSVTIIFDLYGITDGKAVGTNYLGKKYLYTPGSAASGIDFP
CCCEEEEEEECCEEEEEECCEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ELEVDLKVKYIGQPQSSCPF
CEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8367311 [H]