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The map label for this gene is ngoBIM [H]

Identifier: 113478034

GI number: 113478034

Start: 7153316

End: 7154557

Strand: Reverse

Name: ngoBIM [H]

Synonym: Tery_4652

Alternate gene names: 113478034

Gene position: 7154557-7153316 (Counterclockwise)

Preceding gene: 113478035

Following gene: 113478033

Centisome position: 92.32

GC content: 30.68

Gene sequence:

>1242_bases
ATGGAGAAATTTATACTCTACATTTACAGGGCTTTTTATATTTCTGTCTGGCTAAAAGGGAGAATAAAGTTGACTCAAAA
TAGACTAAAAGCCATAGAATTATTTGCTGGAATAGGTGGCTTTTGTTTGGGAATGAGGGCTGCGAATATTAAGACTATTT
GGGCGAATGATGTTAGTAAACTTTGTTGTCAAGTTTATCAAAGTAATTTTGGTAGTAGTTCAATAGTCTTAGATGATATT
AATAAAATTAATTTATTAGAAATTCCAGAACACGATATTTTAACAGCGGGATTTCCCTGTCAACCATTTAGTCAAGCTGG
TAAAAAAATGGGAATTAGAGATAGACTCAGGGGAACACTTTTTGAACGAATTATTGAAATTATCCAAGCTAAAAAGCCGA
AATATTTTTTATTAGAAAATGTTAAAAGAATTTTGACTATGGAAAAAGGGTATCATTTCCGAATTATTTTAAATGCCCTT
GCTTCGTTAGACTACTTTATTGAATGGCGAATCATTAACCCTATAAATTTTAGTATTCCCCAGAACCGAGACAGAATTTT
TATTTTTGGTACTTGTATTAAGTCTACACAAAAAACTCTCGATCTAGAAAACCTTTCTGTATTTTTAACAGATAATGATA
TTCAGAGTTTACCTTGGAATGAAAATAGCGAAATAGAACAATATATGAAGGCTATTATTGATTTTAGTAGTAAAAACTAT
CACTGGGGTATTGCTTATCAAAATCAAATGTATAGTCAATCTTTGCCTTTAATGGCTGATATAAAACCCAGACAAAAACT
TAAAGATATTCTACAATATGAATCAGAAATAAACCCACAATTTTATTTTACTTCTGAAACCCTAGAAAGAATCAAACAGA
GTAAAGCAGTAAATAGATATTATAATGGTGTGGAAATATTATATAATCAAGGTGGAGGAGCGCGTCTTGGCTATACAATA
TTTGGCATTAATGGAGTTACTTCTACATTAACTTCTTCTACTTCTAGGCATTATGAAAGATATCAAGTAGGTAATAAATT
TCGTCGTTTAACTAATATTGAATATGCTCGTTTAATGGGATTTCCTGATAATTGGTGTCGAGTTGTTAAAATTTATCACC
AATATGCTTTATATGGTAATTCGGTTGTGCCTGATTGTGTAGAATGGATATGTAAAAGAATTGGCAGACAAAACTTTAGA
TTTAAGCCTCAAACTTGGCAACAGTTAACGTTATTTAGTTAA

Upstream 100 bases:

>100_bases
CCTTGCTCTTAGCACCTTTACTGAAGTATATAAGGACAAAGCTCATGAACACTTTGTTGGAGGAGTCCGTAAGTCTCGTT
GGTATCAAAGTGTGTAAAAA

Downstream 100 bases:

>100_bases
TTCAATATTTATCAATATATGAAAGAACTTACTCTATTATTATTTAAAACGGAGACCATAACTTTTGTGAGAGATTTAAG
TGTTACTATAATTTTTGATT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoBI; Cytosine-specific methyltransferase NgoBI; M.NgoI [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MEKFILYIYRAFYISVWLKGRIKLTQNRLKAIELFAGIGGFCLGMRAANIKTIWANDVSKLCCQVYQSNFGSSSIVLDDI
NKINLLEIPEHDILTAGFPCQPFSQAGKKMGIRDRLRGTLFERIIEIIQAKKPKYFLLENVKRILTMEKGYHFRIILNAL
ASLDYFIEWRIINPINFSIPQNRDRIFIFGTCIKSTQKTLDLENLSVFLTDNDIQSLPWNENSEIEQYMKAIIDFSSKNY
HWGIAYQNQMYSQSLPLMADIKPRQKLKDILQYESEINPQFYFTSETLERIKQSKAVNRYYNGVEILYNQGGGARLGYTI
FGINGVTSTLTSSTSRHYERYQVGNKFRRLTNIEYARLMGFPDNWCRVVKIYHQYALYGNSVVPDCVEWICKRIGRQNFR
FKPQTWQQLTLFS

Sequences:

>Translated_413_residues
MEKFILYIYRAFYISVWLKGRIKLTQNRLKAIELFAGIGGFCLGMRAANIKTIWANDVSKLCCQVYQSNFGSSSIVLDDI
NKINLLEIPEHDILTAGFPCQPFSQAGKKMGIRDRLRGTLFERIIEIIQAKKPKYFLLENVKRILTMEKGYHFRIILNAL
ASLDYFIEWRIINPINFSIPQNRDRIFIFGTCIKSTQKTLDLENLSVFLTDNDIQSLPWNENSEIEQYMKAIIDFSSKNY
HWGIAYQNQMYSQSLPLMADIKPRQKLKDILQYESEINPQFYFTSETLERIKQSKAVNRYYNGVEILYNQGGGARLGYTI
FGINGVTSTLTSSTSRHYERYQVGNKFRRLTNIEYARLMGFPDNWCRVVKIYHQYALYGNSVVPDCVEWICKRIGRQNFR
FKPQTWQQLTLFS
>Mature_413_residues
MEKFILYIYRAFYISVWLKGRIKLTQNRLKAIELFAGIGGFCLGMRAANIKTIWANDVSKLCCQVYQSNFGSSSIVLDDI
NKINLLEIPEHDILTAGFPCQPFSQAGKKMGIRDRLRGTLFERIIEIIQAKKPKYFLLENVKRILTMEKGYHFRIILNAL
ASLDYFIEWRIINPINFSIPQNRDRIFIFGTCIKSTQKTLDLENLSVFLTDNDIQSLPWNENSEIEQYMKAIIDFSSKNY
HWGIAYQNQMYSQSLPLMADIKPRQKLKDILQYESEINPQFYFTSETLERIKQSKAVNRYYNGVEILYNQGGGARLGYTI
FGINGVTSTLTSSTSRHYERYQVGNKFRRLTNIEYARLMGFPDNWCRVVKIYHQYALYGNSVVPDCVEWICKRIGRQNFR
FKPQTWQQLTLFS

Specific function: This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=399, Percent_Identity=23.0576441102757, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788271, Length=195, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48332; Mature: 48332

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFILYIYRAFYISVWLKGRIKLTQNRLKAIELFAGIGGFCLGMRAANIKTIWANDVSK
CCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEHHHHHH
LCCQVYQSNFGSSSIVLDDINKINLLEIPEHDILTAGFPCQPFSQAGKKMGIRDRLRGTL
HHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCEECCCCCCCHHHHHHCCCHHHHHHHHH
FERIIEIIQAKKPKYFLLENVKRILTMEKGYHFRIILNALASLDYFIEWRIINPINFSIP
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCEEEEEEEECCEECCCC
QNRDRIFIFGTCIKSTQKTLDLENLSVFLTDNDIQSLPWNENSEIEQYMKAIIDFSSKNY
CCCCEEEEEEEEHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCE
HWGIAYQNQMYSQSLPLMADIKPRQKLKDILQYESEINPQFYFTSETLERIKQSKAVNRY
EEEEEECCHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
YNGVEILYNQGGGARLGYTIFGINGVTSTLTSSTSRHYERYQVGNKFRRLTNIEYARLMG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
FPDNWCRVVKIYHQYALYGNSVVPDCVEWICKRIGRQNFRFKPQTWQQLTLFS
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MEKFILYIYRAFYISVWLKGRIKLTQNRLKAIELFAGIGGFCLGMRAANIKTIWANDVSK
CCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEHHHHHH
LCCQVYQSNFGSSSIVLDDINKINLLEIPEHDILTAGFPCQPFSQAGKKMGIRDRLRGTL
HHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCEECCCCCCCHHHHHHCCCHHHHHHHHH
FERIIEIIQAKKPKYFLLENVKRILTMEKGYHFRIILNALASLDYFIEWRIINPINFSIP
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCEEEEEEEECCEECCCC
QNRDRIFIFGTCIKSTQKTLDLENLSVFLTDNDIQSLPWNENSEIEQYMKAIIDFSSKNY
CCCCEEEEEEEEHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCE
HWGIAYQNQMYSQSLPLMADIKPRQKLKDILQYESEINPQFYFTSETLERIKQSKAVNRY
EEEEEECCHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
YNGVEILYNQGGGARLGYTIFGINGVTSTLTSSTSRHYERYQVGNKFRRLTNIEYARLMG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
FPDNWCRVVKIYHQYALYGNSVVPDCVEWICKRIGRQNFRFKPQTWQQLTLFS
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9628358 [H]