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The map label for this gene is tvaII [H]

Identifier: 113477792

GI number: 113477792

Start: 6765304

End: 6766785

Strand: Reverse

Name: tvaII [H]

Synonym: Tery_4392

Alternate gene names: 113477792

Gene position: 6766785-6765304 (Counterclockwise)

Preceding gene: 113477794

Following gene: 113477791

Centisome position: 87.31

GC content: 37.92

Gene sequence:

>1482_bases
ATGCAAATTCAAACACCAGAATGGGTTAAACAGGCTGTATTTTACCAAATTTTTCCAGATCGTTTTGCTAAAGCTCAACC
AACGACTGAAACTGGAAAAGGAATAGACAAAGGTTGGTATCCCAGCAGTCTAGAACCTTGGGATGAATATCCTACTCCTC
AAGGTTATAAAGGAGGGAACCTCTGGGGTGTCATTAAAAAACTTGACTATCTTCAGGAACTTGGTATCACAGCTATTTAC
TTTACACCAATTTTTCAGTCAACCTGTAACCACCGCTATCATACTCATGACTATTATCGAGTCGATCCAATTTTAGGAGG
TAATGAGGCTTTAAGGGTATTAATAGATACTGCTCACGATCGCAATATTAAAGTGGTTCTTGATGGAGTTTTCAACCACG
CTAGCCGGGGTTTTTATTTCTTTAACGATATCCTGGAAAACGGTCCTTATTCTCCCTGGTTGCATTGGTTCAAAATAGAA
AATTGGCCTCTGTCTGCTTACGATGGTAGTTTACCTGCTAATTATGTGGGTTGGTTAGATAACCGTGCTTTACCTGTTTT
TAATCATGACAACCCAGAGGTCAAAGAATACATTATGGAAATTGGAGAATATTGGTTAAAATTTGGTATTGATGGTTGGC
GGTTGGATGTACCTTTTGAAGTGAAGACTCCCGGGTTTTGGCAAGAATTTCGCGATCGCGTTAAGGCTATTAACCCAGAA
GCATATATTGTGGGTGAAGTCTGGAGTAACTGCGCACAATGGTTGGATGGTACTCAGTTTGATGGAGTGATGAATTACTT
ATTTACTGGTCCAACTATTGCTTTTACTGTAGGCGATCGTGTGGATCTTGAGCAAATTGAAGGCCGTGACTATGTAGCTT
ATCCGGCTATATCTGCTGTTGAATACGGTCAAAAAATGCAGGAGTTACTGCAACTTTACCCTTGGGAAATTCAATTAACT
CAACTCAATTTATTAGATAGTCATGATACAGCTAGAGTTTTGAGTATTGCTAAAGGGGATAAAGCAAGTGTTGAATTAGC
GACTCTGTTGTTAATGACTTTTCCCGGAGCACCAAGTATTTATTATGGTGATGAGGTAGGTTTACCGGGTAAGTTTGACC
CTGACTGTCGTCGTAGTTTTCCTACTACTGAAAAATGGGATCGTGAAGTTCTGGAATATCATAAACAGTTAATTGCTATT
AGAAAAGAATATTCAGCTTTGCGCACTGGTAATTACGAAGTGTTGTTTGCCGAGGGAAATGTTTATATTTTTGCTCGTAT
TTTAGAAGAGGAAGAAATTATTGTAGCTGTTAACATTGGTATAGAAGCAGCAAAAGTAAATGTTATTTTTAATAAGCTTA
AATCTCAACCTAATAAAGTTGTTTATGGTAGTTGTAAAATTTCCTGGCAAGGAGGAGATGAAGCTAGTAAATTAGAGTTA
TATTTGCCAGGAAGAAAAGGTTGTATTTTGGTTAAAGATTAA

Upstream 100 bases:

>100_bases
GTCTAACTTTTAACTTTTGACTTCTCTTTAATTGTATAATATTATAAGATTAATTGACTGTCAAAATAGTAGTGGTTTTT
GTTCAAAGAGAAAATTATTT

Downstream 100 bases:

>100_bases
TTGGTGTTATACTTGGGAAAATAGTATATGGAGATGAACAATAGAAACTGGGGATTAAGATTTTATCTCTTTGCACCTAT
AATAAAAATAATATGTAATA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: Alpha-amylase II; TVA II [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MQIQTPEWVKQAVFYQIFPDRFAKAQPTTETGKGIDKGWYPSSLEPWDEYPTPQGYKGGNLWGVIKKLDYLQELGITAIY
FTPIFQSTCNHRYHTHDYYRVDPILGGNEALRVLIDTAHDRNIKVVLDGVFNHASRGFYFFNDILENGPYSPWLHWFKIE
NWPLSAYDGSLPANYVGWLDNRALPVFNHDNPEVKEYIMEIGEYWLKFGIDGWRLDVPFEVKTPGFWQEFRDRVKAINPE
AYIVGEVWSNCAQWLDGTQFDGVMNYLFTGPTIAFTVGDRVDLEQIEGRDYVAYPAISAVEYGQKMQELLQLYPWEIQLT
QLNLLDSHDTARVLSIAKGDKASVELATLLLMTFPGAPSIYYGDEVGLPGKFDPDCRRSFPTTEKWDREVLEYHKQLIAI
RKEYSALRTGNYEVLFAEGNVYIFARILEEEEIIVAVNIGIEAAKVNVIFNKLKSQPNKVVYGSCKISWQGGDEASKLEL
YLPGRKGCILVKD

Sequences:

>Translated_493_residues
MQIQTPEWVKQAVFYQIFPDRFAKAQPTTETGKGIDKGWYPSSLEPWDEYPTPQGYKGGNLWGVIKKLDYLQELGITAIY
FTPIFQSTCNHRYHTHDYYRVDPILGGNEALRVLIDTAHDRNIKVVLDGVFNHASRGFYFFNDILENGPYSPWLHWFKIE
NWPLSAYDGSLPANYVGWLDNRALPVFNHDNPEVKEYIMEIGEYWLKFGIDGWRLDVPFEVKTPGFWQEFRDRVKAINPE
AYIVGEVWSNCAQWLDGTQFDGVMNYLFTGPTIAFTVGDRVDLEQIEGRDYVAYPAISAVEYGQKMQELLQLYPWEIQLT
QLNLLDSHDTARVLSIAKGDKASVELATLLLMTFPGAPSIYYGDEVGLPGKFDPDCRRSFPTTEKWDREVLEYHKQLIAI
RKEYSALRTGNYEVLFAEGNVYIFARILEEEEIIVAVNIGIEAAKVNVIFNKLKSQPNKVVYGSCKISWQGGDEASKLEL
YLPGRKGCILVKD
>Mature_493_residues
MQIQTPEWVKQAVFYQIFPDRFAKAQPTTETGKGIDKGWYPSSLEPWDEYPTPQGYKGGNLWGVIKKLDYLQELGITAIY
FTPIFQSTCNHRYHTHDYYRVDPILGGNEALRVLIDTAHDRNIKVVLDGVFNHASRGFYFFNDILENGPYSPWLHWFKIE
NWPLSAYDGSLPANYVGWLDNRALPVFNHDNPEVKEYIMEIGEYWLKFGIDGWRLDVPFEVKTPGFWQEFRDRVKAINPE
AYIVGEVWSNCAQWLDGTQFDGVMNYLFTGPTIAFTVGDRVDLEQIEGRDYVAYPAISAVEYGQKMQELLQLYPWEIQLT
QLNLLDSHDTARVLSIAKGDKASVELATLLLMTFPGAPSIYYGDEVGLPGKFDPDCRRSFPTTEKWDREVLEYHKQLIAI
RKEYSALRTGNYEVLFAEGNVYIFARILEEEEIIVAVNIGIEAAKVNVIFNKLKSQPNKVVYGSCKISWQGGDEASKLEL
YLPGRKGCILVKD

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=380, Percent_Identity=24.7368421052632, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786604, Length=474, Percent_Identity=36.2869198312236, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1789995, Length=529, Percent_Identity=23.062381852552, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790687, Length=407, Percent_Identity=25.7985257985258, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6322245, Length=439, Percent_Identity=24.373576309795, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321726, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=238, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=238, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=238, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=244, Percent_Identity=25.8196721311475, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=244, Percent_Identity=25.8196721311475, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586599, Length=599, Percent_Identity=21.8697829716194, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583747, Length=578, Percent_Identity=23.5294117647059, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583749, Length=578, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586587, Length=231, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586593, Length=603, Percent_Identity=23.3830845771144, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586589, Length=583, Percent_Identity=21.0977701543739, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221330053, Length=253, Percent_Identity=28.8537549407115, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45549022, Length=600, Percent_Identity=22.5, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586597, Length=429, Percent_Identity=23.7762237762238, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586591, Length=229, Percent_Identity=26.2008733624454, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583745, Length=415, Percent_Identity=24.3373493975904, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 56594; Mature: 56594

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIQTPEWVKQAVFYQIFPDRFAKAQPTTETGKGIDKGWYPSSLEPWDEYPTPQGYKGGN
CCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LWGVIKKLDYLQELGITAIYFTPIFQSTCNHRYHTHDYYRVDPILGGNEALRVLIDTAHD
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCEEEECCEECCCCEEEEEEECCCC
RNIKVVLDGVFNHASRGFYFFNDILENGPYSPWLHWFKIENWPLSAYDGSLPANYVGWLD
CCEEEEEEEHHHHCCCCEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCC
NRALPVFNHDNPEVKEYIMEIGEYWLKFGIDGWRLDVPFEVKTPGFWQEFRDRVKAINPE
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHHCCCC
AYIVGEVWSNCAQWLDGTQFDGVMNYLFTGPTIAFTVGDRVDLEQIEGRDYVAYPAISAV
EEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCEEECCCHHHH
EYGQKMQELLQLYPWEIQLTQLNLLDSHDTARVLSIAKGDKASVELATLLLMTFPGAPSI
HHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHEEEECCCCCCHHHHHHEEHHCCCCCCCE
YYGDEVGLPGKFDPDCRRSFPTTEKWDREVLEYHKQLIAIRKEYSALRTGNYEVLFAEGN
EECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
VYIFARILEEEEIIVAVNIGIEAAKVNVIFNKLKSQPNKVVYGSCKISWQGGDEASKLEL
EEEEEEEECCCCEEEEEECCCEEEHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEE
YLPGRKGCILVKD
EECCCCCEEEEEC
>Mature Secondary Structure
MQIQTPEWVKQAVFYQIFPDRFAKAQPTTETGKGIDKGWYPSSLEPWDEYPTPQGYKGGN
CCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LWGVIKKLDYLQELGITAIYFTPIFQSTCNHRYHTHDYYRVDPILGGNEALRVLIDTAHD
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCEEEECCEECCCCEEEEEEECCCC
RNIKVVLDGVFNHASRGFYFFNDILENGPYSPWLHWFKIENWPLSAYDGSLPANYVGWLD
CCEEEEEEEHHHHCCCCEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCC
NRALPVFNHDNPEVKEYIMEIGEYWLKFGIDGWRLDVPFEVKTPGFWQEFRDRVKAINPE
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHHCCCC
AYIVGEVWSNCAQWLDGTQFDGVMNYLFTGPTIAFTVGDRVDLEQIEGRDYVAYPAISAV
EEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCEEECCCHHHH
EYGQKMQELLQLYPWEIQLTQLNLLDSHDTARVLSIAKGDKASVELATLLLMTFPGAPSI
HHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHEEEECCCCCCHHHHHHEEHHCCCCCCCE
YYGDEVGLPGKFDPDCRRSFPTTEKWDREVLEYHKQLIAIRKEYSALRTGNYEVLFAEGN
EECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
VYIFARILEEEEIIVAVNIGIEAAKVNVIFNKLKSQPNKVVYGSCKISWQGGDEASKLEL
EEEEEEEECCCCEEEEEECCCEEEHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEE
YLPGRKGCILVKD
EECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763540; 10222200; 12051850 [H]