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The map label for this gene is pcrA [H]

Identifier: 113477645

GI number: 113477645

Start: 6533207

End: 6535552

Strand: Reverse

Name: pcrA [H]

Synonym: Tery_4232

Alternate gene names: 113477645

Gene position: 6535552-6533207 (Counterclockwise)

Preceding gene: 113477653

Following gene: 113477644

Centisome position: 84.33

GC content: 34.87

Gene sequence:

>2346_bases
ATGCCAAGCACTGACTTTCTGACTCAGCTAAATTTATCCCAACGCCAAGCAGTAGAACATTTCTGCGGACCTATGTTGGT
CGTGGCGGGTGCTGGGTCTGGCAAAACTAGGGCGTTGACTTATCGTGTAGTCCATCTAATACGTCATCATCGGGTACATC
CAGAAAATATTCTGGCGGTTACCTTTACAAATAAAGCTGCACAGGAAATGAAGGACCGCATTGAAAAAGTATTTGCTCAA
GAACAAGCGGAAGCTAAATATAACAAACCATTTTCAGCATTGACATCAGAAGAACAAATCAGGTTGCGATCGCAAGTCTA
CAAAAATATCACTAAACATTTATGGGTGGGAACTTTTCATAATCTTTGCGCTCGTATTCTGAGGTTTGATATTAACAAAT
ATCAGGATGAAAAAAAACGTCATTGGGATAAAAATTTTTCTATATTTGATGAAAGTGATGCTCAAAGTTTAATTAAGCAA
ATTGTCACTAAACAGCTAAATTTGGATGATAAAAAATTTGAACCACGTTCTGTGAGATATGCCATTAGTAATGCTAAAAA
CCAAGGAATGTCACCTCTAGAATATCAAAGAGCCGAGCCAGATTATCGGGGACGGGTAATTGCTGAAGTTTATGGAATTT
ATCAAGATAATTTAGCTGCGAACAATGCTCTTGATTTTGACGACCTAATCAGAATACCAGTAGAATTATTTCGGCAAAAT
GAGCAAATATTAGCTTATTGGTATCAACGTTTTAATCATATTTTAGTAGATGAATATCAAGATACTAATCGGACTCAATA
TAATTTTATTAGATTTTTAGCTACTAATGGTGAAGACCCTAAATATATTAAAAACTGGGAAAATCGTTCTATTTTTGTGG
TAGGAGATGTAGACCAATCTATTTATTCTTTCCGCATGGCAGATTATACAATTTTGCTTGATTTTCAGAATGATTTTGGC
GATGGTTTAGCTGATGAATATACTCAGACAATGATTAAGTTGGAGGAAAATTATCGCTCACGGGAAAATATTTTGGCAGT
GGCAAATAAGTTGATTGAAAATAATACTCAACGTATTGACAAAACTCTCAAACCAACCAGGGGCATAGGGGAAGAAATTT
ATTGTTATGAGGCAGAAAATGAGTTAGAAGAAGCAGAATTTATTTGTAGTAAAATTGCAGAAATTACAGACCAATATCCA
GACTTAGATTTAGGAAGCTTCGCTGTACTTTATCGAACAAACTCTCAGTCACGCTCCCTCGAAGAAAAGTTGATTCATTA
TGATATTAAATATGTCATAATTGGGGGATTGAGATTTTATGATCGGAAAGAAATTAAGGATGCTTTAGCTTATTTACGAG
TCATTGCAAATCCTGCTGATACTGTTAGTTTACTCAGAATTATTAACACTCCGAGAAGGGGTATTGGCAAGGCAACTATT
GATAGTTTATTAAATGCTAGTTCCCAGATGGGAATACCTTTGTGGGAAATTATTAATGATCAAGCTTCAGTAAATGCTTT
GGCAGGTCGTTCATCAAAAGCTGTAAATAAGTTTGCCGAAGTAATTCAACATTTGCAAGATGAGTTAGAAAATTTGACTG
CTCTGGAAATTGTTGAGAGAATTTTAGAAAATTCTGGTTATATTGAAAACTTGAAAAAACAGGATACGGAAGATGCAGAT
AATCGACTGGCAAATTTAGGAGAATTATGTAGTGCTGTAGCTCAGTTTCAAGAAGATAATGAAGATACAACTTTAGGAAG
TTTTTTAGCAAATGCTTCTTTAGCTTCTAATTTAGATAACCTTCAAGATGGGCAGGAAGCTGTGTCTTTAATGACTTTAC
ATTCTGCTAAAGGTTTAGAATTTCCCGTAGTATTTATAGTGGGTTTGGAGCAAGGTTTATTACCTCATTTTCGGAGCATA
AATGACCCTTTATCTTTAGAAGAAGAAAGGCGACTTTGTTATGTAGGTATTACTAGAGCCGAAGAACAATTATTTTTTTC
TTATGCAACTGAAAGACGACAGCTTTGGGGAGCAAGAGATGCCACAGTTCCATCTCAATTTTTGGGAGAATTACCAAGAG
ATTTGATTAATACTAATGGGATGAAAAAAGTAATTTATCCGTCAAAGCATCAAAGGAAAAACACAAAAAATACTGTTGGA
AAAAAATCGGTAAGTAATCAAATAAAAAGTTGGCAAGTCGGGGATAAAGTGATGCATGAAAGTTTTGGTGTGGGGTTAGT
AACAAACATTCTGGGTGAAGGACATAAGATGAGTTTAGGTATCAAATTTGGTAAGAGTAAAAAAATTATTGATCCCAAAA
CGCCATCAATAGAAAAGTTGAATTAA

Upstream 100 bases:

>100_bases
AGGCTTTCAATTCAAGCTGTAAAATAAAATTCAACCTGCTATTTTATTTTAGTCTGGTGATAAAATTCAATATATTATCT
GCTTTATATAAAATCCAAAT

Downstream 100 bases:

>100_bases
AATAGGCAGGGAGTAGTTATAATTAATAGGAACGAGAACAAAAATATAAATTTTATTTGAATTGCATAAAAAACCAGGCT
GCTTTTAGACAATGGTTTGA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 781; Mature: 780

Protein sequence:

>781_residues
MPSTDFLTQLNLSQRQAVEHFCGPMLVVAGAGSGKTRALTYRVVHLIRHHRVHPENILAVTFTNKAAQEMKDRIEKVFAQ
EQAEAKYNKPFSALTSEEQIRLRSQVYKNITKHLWVGTFHNLCARILRFDINKYQDEKKRHWDKNFSIFDESDAQSLIKQ
IVTKQLNLDDKKFEPRSVRYAISNAKNQGMSPLEYQRAEPDYRGRVIAEVYGIYQDNLAANNALDFDDLIRIPVELFRQN
EQILAYWYQRFNHILVDEYQDTNRTQYNFIRFLATNGEDPKYIKNWENRSIFVVGDVDQSIYSFRMADYTILLDFQNDFG
DGLADEYTQTMIKLEENYRSRENILAVANKLIENNTQRIDKTLKPTRGIGEEIYCYEAENELEEAEFICSKIAEITDQYP
DLDLGSFAVLYRTNSQSRSLEEKLIHYDIKYVIIGGLRFYDRKEIKDALAYLRVIANPADTVSLLRIINTPRRGIGKATI
DSLLNASSQMGIPLWEIINDQASVNALAGRSSKAVNKFAEVIQHLQDELENLTALEIVERILENSGYIENLKKQDTEDAD
NRLANLGELCSAVAQFQEDNEDTTLGSFLANASLASNLDNLQDGQEAVSLMTLHSAKGLEFPVVFIVGLEQGLLPHFRSI
NDPLSLEEERRLCYVGITRAEEQLFFSYATERRQLWGARDATVPSQFLGELPRDLINTNGMKKVIYPSKHQRKNTKNTVG
KKSVSNQIKSWQVGDKVMHESFGVGLVTNILGEGHKMSLGIKFGKSKKIIDPKTPSIEKLN

Sequences:

>Translated_781_residues
MPSTDFLTQLNLSQRQAVEHFCGPMLVVAGAGSGKTRALTYRVVHLIRHHRVHPENILAVTFTNKAAQEMKDRIEKVFAQ
EQAEAKYNKPFSALTSEEQIRLRSQVYKNITKHLWVGTFHNLCARILRFDINKYQDEKKRHWDKNFSIFDESDAQSLIKQ
IVTKQLNLDDKKFEPRSVRYAISNAKNQGMSPLEYQRAEPDYRGRVIAEVYGIYQDNLAANNALDFDDLIRIPVELFRQN
EQILAYWYQRFNHILVDEYQDTNRTQYNFIRFLATNGEDPKYIKNWENRSIFVVGDVDQSIYSFRMADYTILLDFQNDFG
DGLADEYTQTMIKLEENYRSRENILAVANKLIENNTQRIDKTLKPTRGIGEEIYCYEAENELEEAEFICSKIAEITDQYP
DLDLGSFAVLYRTNSQSRSLEEKLIHYDIKYVIIGGLRFYDRKEIKDALAYLRVIANPADTVSLLRIINTPRRGIGKATI
DSLLNASSQMGIPLWEIINDQASVNALAGRSSKAVNKFAEVIQHLQDELENLTALEIVERILENSGYIENLKKQDTEDAD
NRLANLGELCSAVAQFQEDNEDTTLGSFLANASLASNLDNLQDGQEAVSLMTLHSAKGLEFPVVFIVGLEQGLLPHFRSI
NDPLSLEEERRLCYVGITRAEEQLFFSYATERRQLWGARDATVPSQFLGELPRDLINTNGMKKVIYPSKHQRKNTKNTVG
KKSVSNQIKSWQVGDKVMHESFGVGLVTNILGEGHKMSLGIKFGKSKKIIDPKTPSIEKLN
>Mature_780_residues
PSTDFLTQLNLSQRQAVEHFCGPMLVVAGAGSGKTRALTYRVVHLIRHHRVHPENILAVTFTNKAAQEMKDRIEKVFAQE
QAEAKYNKPFSALTSEEQIRLRSQVYKNITKHLWVGTFHNLCARILRFDINKYQDEKKRHWDKNFSIFDESDAQSLIKQI
VTKQLNLDDKKFEPRSVRYAISNAKNQGMSPLEYQRAEPDYRGRVIAEVYGIYQDNLAANNALDFDDLIRIPVELFRQNE
QILAYWYQRFNHILVDEYQDTNRTQYNFIRFLATNGEDPKYIKNWENRSIFVVGDVDQSIYSFRMADYTILLDFQNDFGD
GLADEYTQTMIKLEENYRSRENILAVANKLIENNTQRIDKTLKPTRGIGEEIYCYEAENELEEAEFICSKIAEITDQYPD
LDLGSFAVLYRTNSQSRSLEEKLIHYDIKYVIIGGLRFYDRKEIKDALAYLRVIANPADTVSLLRIINTPRRGIGKATID
SLLNASSQMGIPLWEIINDQASVNALAGRSSKAVNKFAEVIQHLQDELENLTALEIVERILENSGYIENLKKQDTEDADN
RLANLGELCSAVAQFQEDNEDTTLGSFLANASLASNLDNLQDGQEAVSLMTLHSAKGLEFPVVFIVGLEQGLLPHFRSIN
DPLSLEEERRLCYVGITRAEEQLFFSYATERRQLWGARDATVPSQFLGELPRDLINTNGMKKVIYPSKHQRKNTKNTVGK
KSVSNQIKSWQVGDKVMHESFGVGLVTNILGEGHKMSLGIKFGKSKKIIDPKTPSIEKLN

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=770, Percent_Identity=38.0519480519481, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994965, Length=727, Percent_Identity=35.9009628610729, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1787196, Length=397, Percent_Identity=26.1964735516373, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=788, Percent_Identity=27.6649746192893, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6324477, Length=476, Percent_Identity=24.1596638655462, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 89395; Mature: 89264

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTDFLTQLNLSQRQAVEHFCGPMLVVAGAGSGKTRALTYRVVHLIRHHRVHPENILAV
CCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
TFTNKAAQEMKDRIEKVFAQEQAEAKYNKPFSALTSEEQIRLRSQVYKNITKHLWVGTFH
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NLCARILRFDINKYQDEKKRHWDKNFSIFDESDAQSLIKQIVTKQLNLDDKKFEPRSVRY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AISNAKNQGMSPLEYQRAEPDYRGRVIAEVYGIYQDNLAANNALDFDDLIRIPVELFRQN
HHHCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
EQILAYWYQRFNHILVDEYQDTNRTQYNFIRFLATNGEDPKYIKNWENRSIFVVGDVDQS
HHHHHHHHHHHCCEEEECCCCCCCCHHHEEEEEECCCCCCHHHHCCCCCEEEEEECCHHH
IYSFRMADYTILLDFQNDFGDGLADEYTQTMIKLEENYRSRENILAVANKLIENNTQRID
HHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KTLKPTRGIGEEIYCYEAENELEEAEFICSKIAEITDQYPDLDLGSFAVLYRTNSQSRSL
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHH
EEKLIHYDIKYVIIGGLRFYDRKEIKDALAYLRVIANPADTVSLLRIINTPRRGIGKATI
HHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHH
DSLLNASSQMGIPLWEIINDQASVNALAGRSSKAVNKFAEVIQHLQDELENLTALEIVER
HHHHCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILENSGYIENLKKQDTEDADNRLANLGELCSAVAQFQEDNEDTTLGSFLANASLASNLDN
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHH
LQDGQEAVSLMTLHSAKGLEFPVVFIVGLEQGLLPHFRSINDPLSLEEERRLCYVGITRA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEECHH
EEQLFFSYATERRQLWGARDATVPSQFLGELPRDLINTNGMKKVIYPSKHQRKNTKNTVG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCCCEEEECCCHHHCCCHHHHHH
KKSVSNQIKSWQVGDKVMHESFGVGLVTNILGEGHKMSLGIKFGKSKKIIDPKTPSIEKL
HHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCC
N
C
>Mature Secondary Structure 
PSTDFLTQLNLSQRQAVEHFCGPMLVVAGAGSGKTRALTYRVVHLIRHHRVHPENILAV
CCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
TFTNKAAQEMKDRIEKVFAQEQAEAKYNKPFSALTSEEQIRLRSQVYKNITKHLWVGTFH
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NLCARILRFDINKYQDEKKRHWDKNFSIFDESDAQSLIKQIVTKQLNLDDKKFEPRSVRY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AISNAKNQGMSPLEYQRAEPDYRGRVIAEVYGIYQDNLAANNALDFDDLIRIPVELFRQN
HHHCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCC
EQILAYWYQRFNHILVDEYQDTNRTQYNFIRFLATNGEDPKYIKNWENRSIFVVGDVDQS
HHHHHHHHHHHCCEEEECCCCCCCCHHHEEEEEECCCCCCHHHHCCCCCEEEEEECCHHH
IYSFRMADYTILLDFQNDFGDGLADEYTQTMIKLEENYRSRENILAVANKLIENNTQRID
HHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KTLKPTRGIGEEIYCYEAENELEEAEFICSKIAEITDQYPDLDLGSFAVLYRTNSQSRSL
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHH
EEKLIHYDIKYVIIGGLRFYDRKEIKDALAYLRVIANPADTVSLLRIINTPRRGIGKATI
HHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHH
DSLLNASSQMGIPLWEIINDQASVNALAGRSSKAVNKFAEVIQHLQDELENLTALEIVER
HHHHCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILENSGYIENLKKQDTEDADNRLANLGELCSAVAQFQEDNEDTTLGSFLANASLASNLDN
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHH
LQDGQEAVSLMTLHSAKGLEFPVVFIVGLEQGLLPHFRSINDPLSLEEERRLCYVGITRA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEECHH
EEQLFFSYATERRQLWGARDATVPSQFLGELPRDLINTNGMKKVIYPSKHQRKNTKNTVG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCCCEEEECCCHHHCCCHHHHHH
KKSVSNQIKSWQVGDKVMHESFGVGLVTNILGEGHKMSLGIKFGKSKKIIDPKTPSIEKL
HHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]