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The map label for this gene is trmH [C]

Identifier: 113476712

GI number: 113476712

Start: 4845808

End: 4846593

Strand: Reverse

Name: trmH [C]

Synonym: Tery_3173

Alternate gene names: 113476712

Gene position: 4846593-4845808 (Counterclockwise)

Preceding gene: 113476713

Following gene: 113476711

Centisome position: 62.54

GC content: 33.97

Gene sequence:

>786_bases
GTGTTGACTAGTTTACAGAATTCTTTGGTAAAGCAAATTCGCCAACTTCACCAATCAAAAGGGAGGCAGGAACAGCAATT
ATTTCTAGTTGAAGGAACTCATCTCCTGGAAGAAGCTTGCAAGAGAAATTATCCTCTTATAACTGTCTGCCATACATTTG
AATGGCAAGAAAAACATAAATTTTTGTATGAATTAGTATCTCAAAAAGCTCAAAGGATAGAATTAGTAAGTACCAAGATT
ATTCAATCCCTTGCTACTACAGTTAGTCCTGATGGAGTAGTGGCTATAGCACCACAGAGTCAGACAAATTTTTTGAAGAT
TACGACTTTGGGAATTGTTTTAGATAAAATTCAAGACCCTGGTAATTTGGGAACTATAATTAGGACAGCTGTAGCAACAA
ATATAGATGGTTTATTCGTAAGTAGAGATAGTGTAGACTTGTCTCATCCTAAAGTAGTTCGGGCGTCAGCTGGGGAATGG
TTTAATTTACATAAGGCAATATCTGAAAACCTAAAGAATGAAATATTAAAATTACAAAGTCAAGGTCTACAAGTTTTAGC
GACTGTTCCAAATTCAAATATTAACTATTGGGAAATAGACTTACGAAAACCTACAATAATAGTTTTGGGAAATGAGGGTT
CTGGTATTTCTAAAGATTTAATTGATTTAGCTGATTATTCTGTGAAAATTCCTTTGAGTAATGGAGTAGAATCTTTGAAT
GTTGCTGTAAGTGCAGCTATAATTTTGTATGAGATTCAACGTCAACGTCAACTTGAATTAAAGTAG

Upstream 100 bases:

>100_bases
AAACTGGATAATATCTCCGGAACTTTTTATTAATAAGAAGTATCCCGAGCTTCTTTCACTTATAAGATAGAAATGCTAAA
TAAGGGATAAAAGATCAACA

Downstream 100 bases:

>100_bases
TCTGTTTTTATGGTTTAATTTGCAGATAGGTAGTGTTTGGTGTTTCTTATCTGTCTAAATATATTTATCTATGTCAAAAA
AGTTTTTATACTAATTATCT

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLTSLQNSLVKQIRQLHQSKGRQEQQLFLVEGTHLLEEACKRNYPLITVCHTFEWQEKHKFLYELVSQKAQRIELVSTKI
IQSLATTVSPDGVVAIAPQSQTNFLKITTLGIVLDKIQDPGNLGTIIRTAVATNIDGLFVSRDSVDLSHPKVVRASAGEW
FNLHKAISENLKNEILKLQSQGLQVLATVPNSNINYWEIDLRKPTIIVLGNEGSGISKDLIDLADYSVKIPLSNGVESLN
VAVSAAIILYEIQRQRQLELK

Sequences:

>Translated_261_residues
MLTSLQNSLVKQIRQLHQSKGRQEQQLFLVEGTHLLEEACKRNYPLITVCHTFEWQEKHKFLYELVSQKAQRIELVSTKI
IQSLATTVSPDGVVAIAPQSQTNFLKITTLGIVLDKIQDPGNLGTIIRTAVATNIDGLFVSRDSVDLSHPKVVRASAGEW
FNLHKAISENLKNEILKLQSQGLQVLATVPNSNINYWEIDLRKPTIIVLGNEGSGISKDLIDLADYSVKIPLSNGVESLN
VAVSAAIILYEIQRQRQLELK
>Mature_261_residues
MLTSLQNSLVKQIRQLHQSKGRQEQQLFLVEGTHLLEEACKRNYPLITVCHTFEWQEKHKFLYELVSQKAQRIELVSTKI
IQSLATTVSPDGVVAIAPQSQTNFLKITTLGIVLDKIQDPGNLGTIIRTAVATNIDGLFVSRDSVDLSHPKVVRASAGEW
FNLHKAISENLKNEILKLQSQGLQVLATVPNSNINYWEIDLRKPTIIVLGNEGSGISKDLIDLADYSVKIPLSNGVESLN
VAVSAAIILYEIQRQRQLELK

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=163, Percent_Identity=34.3558282208589, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1790083, Length=151, Percent_Identity=33.7748344370861, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790623, Length=147, Percent_Identity=29.9319727891156, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=182, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29129; Mature: 29129

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSLQNSLVKQIRQLHQSKGRQEQQLFLVEGTHLLEEACKRNYPLITVCHTFEWQEKHK
CCCHHHHHHHHHHHHHHHHCCCCHHEEEEEECHHHHHHHHHCCCCEEEEEECCCHHHHHH
FLYELVSQKAQRIELVSTKIIQSLATTVSPDGVVAIAPQSQTNFLKITTLGIVLDKIQDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHHHHCCCC
GNLGTIIRTAVATNIDGLFVSRDSVDLSHPKVVRASAGEWFNLHKAISENLKNEILKLQS
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
QGLQVLATVPNSNINYWEIDLRKPTIIVLGNEGSGISKDLIDLADYSVKIPLSNGVESLN
CCCEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCHHHH
VAVSAAIILYEIQRQRQLELK
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTSLQNSLVKQIRQLHQSKGRQEQQLFLVEGTHLLEEACKRNYPLITVCHTFEWQEKHK
CCCHHHHHHHHHHHHHHHHCCCCHHEEEEEECHHHHHHHHHCCCCEEEEEECCCHHHHHH
FLYELVSQKAQRIELVSTKIIQSLATTVSPDGVVAIAPQSQTNFLKITTLGIVLDKIQDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEHHHHHHHHCCCC
GNLGTIIRTAVATNIDGLFVSRDSVDLSHPKVVRASAGEWFNLHKAISENLKNEILKLQS
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
QGLQVLATVPNSNINYWEIDLRKPTIIVLGNEGSGISKDLIDLADYSVKIPLSNGVESLN
CCCEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCHHHH
VAVSAAIILYEIQRQRQLELK
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]