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The map label for this gene is rluE [H]

Identifier: 113476033

GI number: 113476033

Start: 3711108

End: 3711713

Strand: Direct

Name: rluE [H]

Synonym: Tery_2403

Alternate gene names: 113476033

Gene position: 3711108-3711713 (Clockwise)

Preceding gene: 113476032

Following gene: 113476036

Centisome position: 47.88

GC content: 37.62

Gene sequence:

>606_bases
ATGAAATTTATGGTTTATCGCTATATTTTATTTAATAAGCCTTATAATGTTTTGAGTCAGTTTACTGATAACCGTAATGT
TAAGCATCGATCTTCTACTCTTAAAGAGTATATTTCAGTTGATTCAGTTTATCCAGTTGGCAGATTAGACCAAGATAGTG
AAGGTCTTTTATTATTAACAAACCATGGACAGCTTCAATATCGACTAAGTGATCCTAAGTTTGCTCATCCTCGTACTTAT
TGGGTACAAGTAGAACGAATACCAGATACAATAGCATTAAAGCAACTACAAGAGGGTGTAATGATCAAAAATTATCAAAC
ACGACCCGCAAAAGTTAAATTGTTATCTAACGAACCTCTGTTACCATCGCGCCATCCACCAATTCGATTTCGCAAAACTG
TACCTACTGCATGGTTAGAAATCACTCTCACAGAAGGTCGAAATCGTCAAGTACGAAGGATGACAGCAGCAGTAGGTTTT
CCTACACTTCGGTTAGTTAGGATAGCGATCGCTAATCTTCGTCTTGAGGGACTAAAACCTGGCGAGTGGCGAGACCTAAA
TTTAAGTGAAATTCAATCTCTCAAGAACTACCTCGGCTTAGGATAA

Upstream 100 bases:

>100_bases
AAAATAGCATCTGAAGCCCTCTATAAAATCCAGAGATAAGGACGTAATTATACGATTATTAAAACTTTTTGCACTACTAA
TTTTTCTAATTATATTATAG

Downstream 100 bases:

>100_bases
CAAATTGATTCTGGTTTCAATTGTGCTTGATGTTCACACTGAAAACCTTTCTGACACCATTGTGTCATATTTACCTGTGA
GAGTTTGACCACATCTGTAC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKFMVYRYILFNKPYNVLSQFTDNRNVKHRSSTLKEYISVDSVYPVGRLDQDSEGLLLLTNHGQLQYRLSDPKFAHPRTY
WVQVERIPDTIALKQLQEGVMIKNYQTRPAKVKLLSNEPLLPSRHPPIRFRKTVPTAWLEITLTEGRNRQVRRMTAAVGF
PTLRLVRIAIANLRLEGLKPGEWRDLNLSEIQSLKNYLGLG

Sequences:

>Translated_201_residues
MKFMVYRYILFNKPYNVLSQFTDNRNVKHRSSTLKEYISVDSVYPVGRLDQDSEGLLLLTNHGQLQYRLSDPKFAHPRTY
WVQVERIPDTIALKQLQEGVMIKNYQTRPAKVKLLSNEPLLPSRHPPIRFRKTVPTAWLEITLTEGRNRQVRRMTAAVGF
PTLRLVRIAIANLRLEGLKPGEWRDLNLSEIQSLKNYLGLG
>Mature_201_residues
MKFMVYRYILFNKPYNVLSQFTDNRNVKHRSSTLKEYISVDSVYPVGRLDQDSEGLLLLTNHGQLQYRLSDPKFAHPRTY
WVQVERIPDTIALKQLQEGVMIKNYQTRPAKVKLLSNEPLLPSRHPPIRFRKTVPTAWLEITLTEGRNRQVRRMTAAVGF
PTLRLVRIAIANLRLEGLKPGEWRDLNLSEIQSLKNYLGLG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=180, Percent_Identity=56.1111111111111, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1790453, Length=186, Percent_Identity=31.1827956989247, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788510, Length=192, Percent_Identity=32.2916666666667, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787524, Length=156, Percent_Identity=33.974358974359, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23402; Mature: 23402

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFMVYRYILFNKPYNVLSQFTDNRNVKHRSSTLKEYISVDSVYPVGRLDQDSEGLLLLT
CCEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
NHGQLQYRLSDPKFAHPRTYWVQVERIPDTIALKQLQEGVMIKNYQTRPAKVKLLSNEPL
CCCEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCC
LPSRHPPIRFRKTVPTAWLEITLTEGRNRQVRRMTAAVGFPTLRLVRIAIANLRLEGLKP
CCCCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHEEECCCCC
GEWRDLNLSEIQSLKNYLGLG
CCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFMVYRYILFNKPYNVLSQFTDNRNVKHRSSTLKEYISVDSVYPVGRLDQDSEGLLLLT
CCEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
NHGQLQYRLSDPKFAHPRTYWVQVERIPDTIALKQLQEGVMIKNYQTRPAKVKLLSNEPL
CCCEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCC
LPSRHPPIRFRKTVPTAWLEITLTEGRNRQVRRMTAAVGFPTLRLVRIAIANLRLEGLKP
CCCCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHEEECCCCC
GEWRDLNLSEIQSLKNYLGLG
CCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]