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The map label for this gene is pdhC [H]

Identifier: 113475499

GI number: 113475499

Start: 2795958

End: 2797253

Strand: Direct

Name: pdhC [H]

Synonym: Tery_1831

Alternate gene names: 113475499

Gene position: 2795958-2797253 (Clockwise)

Preceding gene: 113475498

Following gene: 113475500

Centisome position: 36.08

GC content: 42.36

Gene sequence:

>1296_bases
ATGATCAAAGAAATATTTATGCCTGCCCTTAGTTCTACCATGACCGAGGGTAAAATTGTTTCTTGGCAAAAAACTTCCGG
TGATTGGGTAGAAAAGGGCGAAACAGTTGTGGTGGTTGAATCAGACAAAGCAGATATGGATGTGGAATCCTTCTTTTCTG
GATATTTGGCCACAATTATTGTGGAAGCAGGAGATGTCGCTCCAGTTGGATCTACTATTGGTTTGTTAGCTGAAACAGAG
GCAGAAATTGAGCAGGCAAAGCAACAAGGTGTGACTACTCTAAACAAAGAACCTGCTAATACTTCTAGCTCCACAACTCC
TGTGGCCACAGCTCCTATTTCTACAGCCACAGAAAACCAAGAAAATTCTAGTCGTCGTAATGGACGAATTATTGCTTCCC
CTCGTGCCCGGAAGTTGGCCAAAGATTTGAAGGTCGATTTGAGTACCCTCAAAGGTAATGGTCCTCATGGTCGTATTGTG
GCAGAGGATGTGGAAATGGCGGCGGGTCGTATACCTGCTGTGGTAGCTGCATCAGCAAAGAGTACTATTCCTACTACTCC
AACTCAAGTATCTATTCCTGCTCCACCACCACCACCATCTGTAGTGTCTGCTCCAGTAACTCCTGGTCAAGTTGTGCCAA
TGAATAGTCTGCAAAATGCAGTGGTGCGGAATATGAATGTAAGTTTATCTGTACCGACTTTCCATGTTGGTTATACTATA
ACTACAGATAATTTGGATAGGTTGTACAAACAAATTAAGTCTAAGGGTGTGACTATGACTGCTATTTTGGCAAAAGCAGT
GGCAATAACCTTACAAAAACATCCTTTGTTGAATGCTGTTTATGTGGATCAGGGTATTCAGTATCCCTCTGGGATTAATA
TTGCAGTGGCAGTAGCAATGCCAGATGGTGGTTTGATTACACCAGTATTGCCAAATGCTGACAAAATGGATATTTATTCT
TTGTCTCGTACTTGGAAAGGTTTGGTAGATAGGGCGCGGGCAAAACAATTACAAGCAAATGAATACAGCACTGGTACCTT
TACTATTTCTAATTTGGGAATGTTTGGGGTAAATAGGTTTGATGCTATTTTACCACCTGCTCAAGGTTCGATTTTGGCGA
TCGGCGCATCTCAACCCCAGGTAGTGGCCACTGATGATGGGATGATTGGGGTTAAGCGACAAATGGAGGTGAATATTACT
TGTGACCATCGGATTATTTATGGTGCTGATGCTGCTGCTTTCTTGCAAGATTTGGCGAATTTGATTGAAAATAATTCACA
GTCTTTGACTATGTAG

Upstream 100 bases:

>100_bases
TAAATTAATAAATTCTGACTCCCGACTTCTGAACTCTTTTTTCAAAAATTCTTTCTCAACAAGTCATCAACAAAAAAACT
ATAAATTATAGAGAACTAAC

Downstream 100 bases:

>100_bases
TTCAGTAGTAGGGTAGCTATCAGTTTTTAGTCACTATTGGTAGCGTTATAGGGTTTGTAGTTTGAAATTCAAAAGCTACT
AATCATATTACAACCCAATG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIKEIFMPALSSTMTEGKIVSWQKTSGDWVEKGETVVVVESDKADMDVESFFSGYLATIIVEAGDVAPVGSTIGLLAETE
AEIEQAKQQGVTTLNKEPANTSSSTTPVATAPISTATENQENSSRRNGRIIASPRARKLAKDLKVDLSTLKGNGPHGRIV
AEDVEMAAGRIPAVVAASAKSTIPTTPTQVSIPAPPPPPSVVSAPVTPGQVVPMNSLQNAVVRNMNVSLSVPTFHVGYTI
TTDNLDRLYKQIKSKGVTMTAILAKAVAITLQKHPLLNAVYVDQGIQYPSGINIAVAVAMPDGGLITPVLPNADKMDIYS
LSRTWKGLVDRARAKQLQANEYSTGTFTISNLGMFGVNRFDAILPPAQGSILAIGASQPQVVATDDGMIGVKRQMEVNIT
CDHRIIYGADAAAFLQDLANLIENNSQSLTM

Sequences:

>Translated_431_residues
MIKEIFMPALSSTMTEGKIVSWQKTSGDWVEKGETVVVVESDKADMDVESFFSGYLATIIVEAGDVAPVGSTIGLLAETE
AEIEQAKQQGVTTLNKEPANTSSSTTPVATAPISTATENQENSSRRNGRIIASPRARKLAKDLKVDLSTLKGNGPHGRIV
AEDVEMAAGRIPAVVAASAKSTIPTTPTQVSIPAPPPPPSVVSAPVTPGQVVPMNSLQNAVVRNMNVSLSVPTFHVGYTI
TTDNLDRLYKQIKSKGVTMTAILAKAVAITLQKHPLLNAVYVDQGIQYPSGINIAVAVAMPDGGLITPVLPNADKMDIYS
LSRTWKGLVDRARAKQLQANEYSTGTFTISNLGMFGVNRFDAILPPAQGSILAIGASQPQVVATDDGMIGVKRQMEVNIT
CDHRIIYGADAAAFLQDLANLIENNSQSLTM
>Mature_431_residues
MIKEIFMPALSSTMTEGKIVSWQKTSGDWVEKGETVVVVESDKADMDVESFFSGYLATIIVEAGDVAPVGSTIGLLAETE
AEIEQAKQQGVTTLNKEPANTSSSTTPVATAPISTATENQENSSRRNGRIIASPRARKLAKDLKVDLSTLKGNGPHGRIV
AEDVEMAAGRIPAVVAASAKSTIPTTPTQVSIPAPPPPPSVVSAPVTPGQVVPMNSLQNAVVRNMNVSLSVPTFHVGYTI
TTDNLDRLYKQIKSKGVTMTAILAKAVAITLQKHPLLNAVYVDQGIQYPSGINIAVAVAMPDGGLITPVLPNADKMDIYS
LSRTWKGLVDRARAKQLQANEYSTGTFTISNLGMFGVNRFDAILPPAQGSILAIGASQPQVVATDDGMIGVKRQMEVNIT
CDHRIIYGADAAAFLQDLANLIENNSQSLTM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=30.0904977375566, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=31.0267857142857, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=31.0267857142857, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI110671329, Length=426, Percent_Identity=26.7605633802817, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI19923748, Length=164, Percent_Identity=37.1951219512195, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.1190476190476, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786305, Length=437, Percent_Identity=28.1464530892449, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=24.8847926267281, Blast_Score=115, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=30.9734513274336, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=442, Percent_Identity=26.2443438914027, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=210, Percent_Identity=34.2857142857143, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25146366, Length=166, Percent_Identity=34.3373493975904, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324258, Length=445, Percent_Identity=28.9887640449438, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=25.6880733944954, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129315, Length=446, Percent_Identity=31.1659192825112, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24582497, Length=439, Percent_Identity=30.5239179954442, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=24.3055555555556, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645909, Length=181, Percent_Identity=36.4640883977901, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45694; Mature: 45694

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEIFMPALSSTMTEGKIVSWQKTSGDWVEKGETVVVVESDKADMDVESFFSGYLATII
CCHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHEEEEE
VEAGDVAPVGSTIGLLAETEAEIEQAKQQGVTTLNKEPANTSSSTTPVATAPISTATENQ
EECCCCCCCCCHHHHEECCHHHHHHHHHCCCEECCCCCCCCCCCCCCEEECCCCCCCCCC
ENSSRRNGRIIASPRARKLAKDLKVDLSTLKGNGPHGRIVAEDVEMAAGRIPAVVAASAK
CCCCCCCCEEEECCHHHHHHHHHHHHHHECCCCCCCCCEEEHHHHHHCCCCCEEEEECCC
STIPTTPTQVSIPAPPPPPSVVSAPVTPGQVVPMNSLQNAVVRNMNVSLSVPTFHVGYTI
CCCCCCCCEEECCCCCCCCCCEECCCCCCCEEEHHHHHHHHHHCCCEEEECCEEEEEEEE
TTDNLDRLYKQIKSKGVTMTAILAKAVAITLQKHPLLNAVYVDQGIQYPSGINIAVAVAM
ECCCHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCEEEEEEE
PDGGLITPVLPNADKMDIYSLSRTWKGLVDRARAKQLQANEYSTGTFTISNLGMFGVNRF
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHH
DAILPPAQGSILAIGASQPQVVATDDGMIGVKRQMEVNITCDHRIIYGADAAAFLQDLAN
HHCCCCCCCCEEEEECCCCEEEEECCCCEEEEEEEEEEEEECCEEEECCCHHHHHHHHHH
LIENNSQSLTM
HHCCCCCCCCC
>Mature Secondary Structure
MIKEIFMPALSSTMTEGKIVSWQKTSGDWVEKGETVVVVESDKADMDVESFFSGYLATII
CCHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHEEEEE
VEAGDVAPVGSTIGLLAETEAEIEQAKQQGVTTLNKEPANTSSSTTPVATAPISTATENQ
EECCCCCCCCCHHHHEECCHHHHHHHHHCCCEECCCCCCCCCCCCCCEEECCCCCCCCCC
ENSSRRNGRIIASPRARKLAKDLKVDLSTLKGNGPHGRIVAEDVEMAAGRIPAVVAASAK
CCCCCCCCEEEECCHHHHHHHHHHHHHHECCCCCCCCCEEEHHHHHHCCCCCEEEEECCC
STIPTTPTQVSIPAPPPPPSVVSAPVTPGQVVPMNSLQNAVVRNMNVSLSVPTFHVGYTI
CCCCCCCCEEECCCCCCCCCCEECCCCCCCEEEHHHHHHHHHHCCCEEEECCEEEEEEEE
TTDNLDRLYKQIKSKGVTMTAILAKAVAITLQKHPLLNAVYVDQGIQYPSGINIAVAVAM
ECCCHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCEEEEEEE
PDGGLITPVLPNADKMDIYSLSRTWKGLVDRARAKQLQANEYSTGTFTISNLGMFGVNRF
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHH
DAILPPAQGSILAIGASQPQVVATDDGMIGVKRQMEVNITCDHRIIYGADAAAFLQDLAN
HHCCCCCCCCEEEEECCCCEEEEECCCCEEEEEEEEEEEEECCEEEECCCHHHHHHHHHH
LIENNSQSLTM
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA