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The map label for this gene is narB [H]

Identifier: 113474851

GI number: 113474851

Start: 1676566

End: 1678770

Strand: Direct

Name: narB [H]

Synonym: Tery_1070

Alternate gene names: 113474851

Gene position: 1676566-1678770 (Clockwise)

Preceding gene: 113474850

Following gene: 113474855

Centisome position: 21.63

GC content: 43.54

Gene sequence:

>2205_bases
ATGAAAACTACAAAAACTGCTTGCCCTTATTGTGGCGTAGGCTGCGGATTAGAAGTATCACCACCAGCAACCCCAGGAAA
AGTAACAAATCGCGATAGTAAAGGTAATCCAACTTGGCAAGTACGGGGCGATCGCTCTCATCCATCAAGTCAAGGCAAAG
TTTGTCTCAAAGGTGCTACCGTTGGCGAAGCATTGGATAAAAGTCGCCTTAAGTATCCAATGATGCGCAATACCCTAGAT
GAAGCTTTTCGTCGTGTTACTTGGGAAGAAGCCTTTCGGCGCATTGTTAACCAAATTCAAAAAGTACGTTTTTCTCAGGG
ACCAGATGCAATTTGTATGTATGGGTCTGGTCAGTTCCAAACAGAAGACTATTACGTTGCTCAAAAGTTGCTTAAAGGAT
GTCTGGGAACTAACAACTTTGATGCCAACTCTCGCCTCTGTATGTCTTCAGCAGTATCTGGATACATTCAAAGTTTTGGG
TCTGATGGTCCTCCTTGTTGCTACGATGACTTAGAAAAGACAGACTGTGCATTTTTAATAGGTACTAATACAGCCGAATG
TCATCCTATTATCTTTAACCGTCTCCGGGCATCTAAAAAAAGAAATAAAAAAGTCAAAATGATTGTGGTAGACCCCCGTC
GTACCAAAACCGCAGAAGTAGCAGACCTACATTTGGCAATTCGACCAGGTACAGATATTGACTTACTTAATGGTATAGCT
AATCTGCTCATACAGTGGGGTTTACTGGATGCAATGTTTATAGATGAGTGTACGAAGGGCTTCCCTGAATACGTAAATAT
CATTCGTCAGTATCCACCGGGGCTGGTAGCACGTCATTGTGGAATTCGCTTGGATGAGTTGGAAACAGCCGCTCGTTATT
GGGGAAAATCCGAGAGAGTTTTATCTTTGTGGTCAATGGGGATGAATCAGTCATCAGAGGGTACAGCAAAAGTGCGAACC
TTAATTAATCTTCATTTAATGACAGGTGATATAGGCAAACCAGGCACAGGTCCGTTTTCTCTTACTGGTCAACCAAATGC
CATGGGTGGAAGGGAAGCAGGTGGACTAGCTCATCTTTTGCCTGGTTATCGAATGGTAAAAAATTCTCAACATCGTGCTG
AGGTTGAAAAATTGTGGGAACTAACTCCCGGAAAAATTTCTGATGTACCTGGTCGCAGTGCTTGGGAAATGATTATGGCT
TTGGAACGCAAAGAAATTGGTTGTTTGTGGATTGCGGCCACTAATCCTGCTGTGAGTATGCCTGATTTAAAACGGACTAA
GGCTGCTTTGTTGCGATCGCCTTTTACTATTTATCAAGATGCTTATTATCCCACCGAAACAGCTGAATATGCTCACCTAT
TATTACCGGCAGCTCAGTGGGGAGAAAAAACAGGCACTATGACAAATTCGGAGCGAGTGGTGACTTTGTGTCCGGCATTT
CGTGAACCTCTCAGAGAAGCTAAAGCAGATTGGGAAATCTTTGCCGAGGTAGGTCGCCGTTTAGGATTTACAAATCACTT
TAATTTTGCTGACTCGGAAGCTGTCAGAGCTGAGTTTTTGGAATTGACACGGGATCGCCCCTGTGATATGACAGGCATTA
CCTATCAACGACTCCAAGAAAAAGGTCCGACTCAATGGCCTTGTCCTGATAGTAGTGGCGTATCTCAGGAGCGTCTGTAT
ACTGACTTTCGATTTAATACTCCCGATGGCAGGGCGAGGTTTGCAGCATATTACTCTCAAGGACTAGCAGAACCACCTAA
TCCTGATTATCCATTCATTTTAACAACTGGACGATTATATGGTCATTGGCACACTCAAACCCGTACAGGGCGTATTGAAA
AAATTCGCAAAATGTATCCCCTACCATTTATGGAAATTCATCCCCAGGATGCTCAGAGATTGGAGATTTCAGATAATGAT
TGGGTAGAAGTAAGGTCGCGAAGAGGTTATAGCAGATTTCCAGCCAAAATCACCAAAGCTATTACTCCAGGTACTGTATT
TGTACCGATGCACTGGGGTGCATTATGGGCACATCAGGCAGAGGCAAATACTCTGACTCATCCTCAAGCTTGTCCTGAGT
CTGGACAACCAGAGTTAAAAGCTTGTGCTGTACAGTTACAACCAGTTAAATTTCAAGCAGCTCCTATAGCGCTATGGGCA
AGTAAAAAGTCAACCCCCTTGGGAGAAGTAAAAAGCTCAAAGTAA

Upstream 100 bases:

>100_bases
TTTACTGACATTTTCTCGGTTATGCTGCACCCTTGAGGTATTTTTTACCCCAGAAAATTTGTAAGTACTATTAGTAAGTT
TCCAAAAAATTAAGTGCACT

Downstream 100 bases:

>100_bases
TTTCTATTTCCTGTTTCCTAATTTATCCCTATTTCCCCACCATCCCACCTCTCTCTACTTTGGAGTGTTATAAGAAACCA
GATTTATACAAAACAATGAT

Product: assimilatory nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MKTTKTACPYCGVGCGLEVSPPATPGKVTNRDSKGNPTWQVRGDRSHPSSQGKVCLKGATVGEALDKSRLKYPMMRNTLD
EAFRRVTWEEAFRRIVNQIQKVRFSQGPDAICMYGSGQFQTEDYYVAQKLLKGCLGTNNFDANSRLCMSSAVSGYIQSFG
SDGPPCCYDDLEKTDCAFLIGTNTAECHPIIFNRLRASKKRNKKVKMIVVDPRRTKTAEVADLHLAIRPGTDIDLLNGIA
NLLIQWGLLDAMFIDECTKGFPEYVNIIRQYPPGLVARHCGIRLDELETAARYWGKSERVLSLWSMGMNQSSEGTAKVRT
LINLHLMTGDIGKPGTGPFSLTGQPNAMGGREAGGLAHLLPGYRMVKNSQHRAEVEKLWELTPGKISDVPGRSAWEMIMA
LERKEIGCLWIAATNPAVSMPDLKRTKAALLRSPFTIYQDAYYPTETAEYAHLLLPAAQWGEKTGTMTNSERVVTLCPAF
REPLREAKADWEIFAEVGRRLGFTNHFNFADSEAVRAEFLELTRDRPCDMTGITYQRLQEKGPTQWPCPDSSGVSQERLY
TDFRFNTPDGRARFAAYYSQGLAEPPNPDYPFILTTGRLYGHWHTQTRTGRIEKIRKMYPLPFMEIHPQDAQRLEISDND
WVEVRSRRGYSRFPAKITKAITPGTVFVPMHWGALWAHQAEANTLTHPQACPESGQPELKACAVQLQPVKFQAAPIALWA
SKKSTPLGEVKSSK

Sequences:

>Translated_734_residues
MKTTKTACPYCGVGCGLEVSPPATPGKVTNRDSKGNPTWQVRGDRSHPSSQGKVCLKGATVGEALDKSRLKYPMMRNTLD
EAFRRVTWEEAFRRIVNQIQKVRFSQGPDAICMYGSGQFQTEDYYVAQKLLKGCLGTNNFDANSRLCMSSAVSGYIQSFG
SDGPPCCYDDLEKTDCAFLIGTNTAECHPIIFNRLRASKKRNKKVKMIVVDPRRTKTAEVADLHLAIRPGTDIDLLNGIA
NLLIQWGLLDAMFIDECTKGFPEYVNIIRQYPPGLVARHCGIRLDELETAARYWGKSERVLSLWSMGMNQSSEGTAKVRT
LINLHLMTGDIGKPGTGPFSLTGQPNAMGGREAGGLAHLLPGYRMVKNSQHRAEVEKLWELTPGKISDVPGRSAWEMIMA
LERKEIGCLWIAATNPAVSMPDLKRTKAALLRSPFTIYQDAYYPTETAEYAHLLLPAAQWGEKTGTMTNSERVVTLCPAF
REPLREAKADWEIFAEVGRRLGFTNHFNFADSEAVRAEFLELTRDRPCDMTGITYQRLQEKGPTQWPCPDSSGVSQERLY
TDFRFNTPDGRARFAAYYSQGLAEPPNPDYPFILTTGRLYGHWHTQTRTGRIEKIRKMYPLPFMEIHPQDAQRLEISDND
WVEVRSRRGYSRFPAKITKAITPGTVFVPMHWGALWAHQAEANTLTHPQACPESGQPELKACAVQLQPVKFQAAPIALWA
SKKSTPLGEVKSSK
>Mature_734_residues
MKTTKTACPYCGVGCGLEVSPPATPGKVTNRDSKGNPTWQVRGDRSHPSSQGKVCLKGATVGEALDKSRLKYPMMRNTLD
EAFRRVTWEEAFRRIVNQIQKVRFSQGPDAICMYGSGQFQTEDYYVAQKLLKGCLGTNNFDANSRLCMSSAVSGYIQSFG
SDGPPCCYDDLEKTDCAFLIGTNTAECHPIIFNRLRASKKRNKKVKMIVVDPRRTKTAEVADLHLAIRPGTDIDLLNGIA
NLLIQWGLLDAMFIDECTKGFPEYVNIIRQYPPGLVARHCGIRLDELETAARYWGKSERVLSLWSMGMNQSSEGTAKVRT
LINLHLMTGDIGKPGTGPFSLTGQPNAMGGREAGGLAHLLPGYRMVKNSQHRAEVEKLWELTPGKISDVPGRSAWEMIMA
LERKEIGCLWIAATNPAVSMPDLKRTKAALLRSPFTIYQDAYYPTETAEYAHLLLPAAQWGEKTGTMTNSERVVTLCPAF
REPLREAKADWEIFAEVGRRLGFTNHFNFADSEAVRAEFLELTRDRPCDMTGITYQRLQEKGPTQWPCPDSSGVSQERLY
TDFRFNTPDGRARFAAYYSQGLAEPPNPDYPFILTTGRLYGHWHTQTRTGRIEKIRKMYPLPFMEIHPQDAQRLEISDND
WVEVRSRRGYSRFPAKITKAITPGTVFVPMHWGALWAHQAEANTLTHPQACPESGQPELKACAVQLQPVKFQAAPIALWA
SKKSTPLGEVKSSK

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=729, Percent_Identity=30.8641975308642, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1788534, Length=544, Percent_Identity=32.3529411764706, Blast_Score=274, Evalue=2e-74,
Organism=Escherichia coli, GI3868720, Length=431, Percent_Identity=27.6102088167053, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI3868719, Length=431, Percent_Identity=26.6821345707657, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1787778, Length=717, Percent_Identity=24.6861924686192, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI87081797, Length=756, Percent_Identity=22.7513227513227, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787870, Length=709, Percent_Identity=25.5289139633286, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI171474008, Length=756, Percent_Identity=22.8835978835979, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI145693196, Length=703, Percent_Identity=23.1863442389758, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI87081994, Length=772, Percent_Identity=21.8911917098446, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 82091; Mature: 82091

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTKTACPYCGVGCGLEVSPPATPGKVTNRDSKGNPTWQVRGDRSHPSSQGKVCLKGAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCC
VGEALDKSRLKYPMMRNTLDEAFRRVTWEEAFRRIVNQIQKVRFSQGPDAICMYGSGQFQ
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TEDYYVAQKLLKGCLGTNNFDANSRLCMSSAVSGYIQSFGSDGPPCCYDDLEKTDCAFLI
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
GTNTAECHPIIFNRLRASKKRNKKVKMIVVDPRRTKTAEVADLHLAIRPGTDIDLLNGIA
ECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHH
NLLIQWGLLDAMFIDECTKGFPEYVNIIRQYPPGLVARHCGIRLDELETAARYWGKSERV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCHHH
LSLWSMGMNQSSEGTAKVRTLINLHLMTGDIGKPGTGPFSLTGQPNAMGGREAGGLAHLL
HHHHHHCCCCCCCCHHHHHHEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHHHC
PGYRMVKNSQHRAEVEKLWELTPGKISDVPGRSAWEMIMALERKEIGCLWIAATNPAVSM
CCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCC
PDLKRTKAALLRSPFTIYQDAYYPTETAEYAHLLLPAAQWGEKTGTMTNSERVVTLCPAF
CCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCEEEECHHH
REPLREAKADWEIFAEVGRRLGFTNHFNFADSEAVRAEFLELTRDRPCDMTGITYQRLQE
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KGPTQWPCPDSSGVSQERLYTDFRFNTPDGRARFAAYYSQGLAEPPNPDYPFILTTGRLY
CCCCCCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE
GHWHTQTRTGRIEKIRKMYPLPFMEIHPQDAQRLEISDNDWVEVRSRRGYSRFPAKITKA
EEEECCCCCCHHHHHHHHCCCCCEEECCCCCCEEECCCCCHHHHHHCCCCCCCCHHHHHH
ITPGTVFVPMHWGALWAHQAEANTLTHPQACPESGQPELKACAVQLQPVKFQAAPIALWA
CCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECEEEECCCEEEEE
SKKSTPLGEVKSSK
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKTTKTACPYCGVGCGLEVSPPATPGKVTNRDSKGNPTWQVRGDRSHPSSQGKVCLKGAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCC
VGEALDKSRLKYPMMRNTLDEAFRRVTWEEAFRRIVNQIQKVRFSQGPDAICMYGSGQFQ
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TEDYYVAQKLLKGCLGTNNFDANSRLCMSSAVSGYIQSFGSDGPPCCYDDLEKTDCAFLI
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
GTNTAECHPIIFNRLRASKKRNKKVKMIVVDPRRTKTAEVADLHLAIRPGTDIDLLNGIA
ECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHH
NLLIQWGLLDAMFIDECTKGFPEYVNIIRQYPPGLVARHCGIRLDELETAARYWGKSERV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCHHH
LSLWSMGMNQSSEGTAKVRTLINLHLMTGDIGKPGTGPFSLTGQPNAMGGREAGGLAHLL
HHHHHHCCCCCCCCHHHHHHEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHHHC
PGYRMVKNSQHRAEVEKLWELTPGKISDVPGRSAWEMIMALERKEIGCLWIAATNPAVSM
CCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCC
PDLKRTKAALLRSPFTIYQDAYYPTETAEYAHLLLPAAQWGEKTGTMTNSERVVTLCPAF
CCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCEEEECHHH
REPLREAKADWEIFAEVGRRLGFTNHFNFADSEAVRAEFLELTRDRPCDMTGITYQRLQE
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KGPTQWPCPDSSGVSQERLYTDFRFNTPDGRARFAAYYSQGLAEPPNPDYPFILTTGRLY
CCCCCCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE
GHWHTQTRTGRIEKIRKMYPLPFMEIHPQDAQRLEISDNDWVEVRSRRGYSRFPAKITKA
EEEECCCCCCHHHHHHHHCCCCCEEECCCCCCEEECCCCCHHHHHHCCCCCCCCHHHHHH
ITPGTVFVPMHWGALWAHQAEANTLTHPQACPESGQPELKACAVQLQPVKFQAAPIALWA
CCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECEEEECCCEEEEE
SKKSTPLGEVKSSK
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]