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The map label for this gene is cca [H]

Identifier: 113474845

GI number: 113474845

Start: 1663280

End: 1664551

Strand: Direct

Name: cca [H]

Synonym: Tery_1064

Alternate gene names: 113474845

Gene position: 1663280-1664551 (Clockwise)

Preceding gene: 113474842

Following gene: 113474847

Centisome position: 21.46

GC content: 33.57

Gene sequence:

>1272_bases
ATGTTGATAAATTCCCTGTCTGCTTTATCACCAGAAAATTGGCCATTTGAGGCAAAAATGCTGCCCCAAAATGTTTACTT
AGTGGGTGGTTCAGTGCGAGATGCTTTACTGAAACGGAAGACTACATATTTAGATTTAGACTTTATTATCCTTGACCAAG
CAGTTAGTATTGCTAGAAATATTGCTAAGGAATATCAAGCTGGTTTTGTGGTTTTGGATGCGGAAAGAAAAATTGCTAGG
GTAGTATTTAAAGGAACTACAGTTGATTTTGCTGAGATTGAAGGTAATAGTTTGAAGGAAGATTTATGGCGACGAGATTT
TACTATAAATGCTATTGCTTATAATCCTTTTACTAATGAATTGATAGATTTATTTAATGCTCTAAAAGATTTGCAAGACG
GTATTATTAGAATGGTAAAATCTGAGAATCTTAAAGAGGATCCTCTCCGACTATTACGGGCATATCGTCAAGCAGCACAA
TTAGGTTTTATTATTTCCCCAGAAACTCAAACAACTATTCGAGAGTTTGCACCTTTAATTACTCAAGTTGCAACAGAAAG
AGTGCAGACAGAATTAGGTTATTTGTTGAGTCATCCCCAAGGAATGATTTGGATAGAAAAGGCTTGGAAAAATGGTTTAC
TCTCAGATTATTTTCCTGCAGCAACAGAAAATTTGGGAATAGGAAAAAAGGTAGAGATAGTAGTAGAAAAATTACAAAAA
AATTGGTCTACTTTAAAAGTAGAGTTAGGGAGTGGGGTGCGCAATACAGTCAAAACGTCTTTATTGGCAATTGCTAAATT
AGCAATATTAGTTAATTCTAACCCACAAAAAGCGGAAGTTGAACTAATGCGGTTAAAGTATAGCAATGCAGAAATTAGAA
GTACAATTGCAGTTCTTAAAAGTTTAGAAAAACTGTCAGAAATAGAAGTTCTTCATATGTCTGTTAGAGAACAATATTTT
TTCTTTCAACAAATAGGAAAAAATTTTCCTGCTTTAGCAGTTGTAGCAGTGGCAAAAGGAATATCGATGGAGGAAATTAA
TTGTTTAATTGAACGTTATATTAATCCAGATGATCTAGTTGCTCATCCCACAACTTTATTGAGTGGAAATGATTTAATTA
CAGCTCTAAAATTACCTAAAGGTCCCATAGTTGGTCAGCTTTTAACTGAGATTCAAATTGCACGAGCTGAAGAGAATATT
TTAACTCAAGATGAAGCTATTAAATTAGCACAAAAATTAATTGCATTCAGGAATGATCCTTCTTCTTTCTAA

Upstream 100 bases:

>100_bases
TGTTTTTCGCATGCCATGAAAAATTTTATCTCATAACCTGGTTTCTGGAAAAGTCCTATAAAAGTTTAACTGCAAAGAAA
TTCATTTTATTTTTGTGGCA

Downstream 100 bases:

>100_bases
ATATGACGTACTTTTTTATTGGTGGATATCTTTGTTATGAAACTACATTTATGAAAAATTATTATTTGCTCCAATACCAA
GGTTGATCCATTAATTGAAT

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MLINSLSALSPENWPFEAKMLPQNVYLVGGSVRDALLKRKTTYLDLDFIILDQAVSIARNIAKEYQAGFVVLDAERKIAR
VVFKGTTVDFAEIEGNSLKEDLWRRDFTINAIAYNPFTNELIDLFNALKDLQDGIIRMVKSENLKEDPLRLLRAYRQAAQ
LGFIISPETQTTIREFAPLITQVATERVQTELGYLLSHPQGMIWIEKAWKNGLLSDYFPAATENLGIGKKVEIVVEKLQK
NWSTLKVELGSGVRNTVKTSLLAIAKLAILVNSNPQKAEVELMRLKYSNAEIRSTIAVLKSLEKLSEIEVLHMSVREQYF
FFQQIGKNFPALAVVAVAKGISMEEINCLIERYINPDDLVAHPTTLLSGNDLITALKLPKGPIVGQLLTEIQIARAEENI
LTQDEAIKLAQKLIAFRNDPSSF

Sequences:

>Translated_423_residues
MLINSLSALSPENWPFEAKMLPQNVYLVGGSVRDALLKRKTTYLDLDFIILDQAVSIARNIAKEYQAGFVVLDAERKIAR
VVFKGTTVDFAEIEGNSLKEDLWRRDFTINAIAYNPFTNELIDLFNALKDLQDGIIRMVKSENLKEDPLRLLRAYRQAAQ
LGFIISPETQTTIREFAPLITQVATERVQTELGYLLSHPQGMIWIEKAWKNGLLSDYFPAATENLGIGKKVEIVVEKLQK
NWSTLKVELGSGVRNTVKTSLLAIAKLAILVNSNPQKAEVELMRLKYSNAEIRSTIAVLKSLEKLSEIEVLHMSVREQYF
FFQQIGKNFPALAVVAVAKGISMEEINCLIERYINPDDLVAHPTTLLSGNDLITALKLPKGPIVGQLLTEIQIARAEENI
LTQDEAIKLAQKLIAFRNDPSSF
>Mature_423_residues
MLINSLSALSPENWPFEAKMLPQNVYLVGGSVRDALLKRKTTYLDLDFIILDQAVSIARNIAKEYQAGFVVLDAERKIAR
VVFKGTTVDFAEIEGNSLKEDLWRRDFTINAIAYNPFTNELIDLFNALKDLQDGIIRMVKSENLKEDPLRLLRAYRQAAQ
LGFIISPETQTTIREFAPLITQVATERVQTELGYLLSHPQGMIWIEKAWKNGLLSDYFPAATENLGIGKKVEIVVEKLQK
NWSTLKVELGSGVRNTVKTSLLAIAKLAILVNSNPQKAEVELMRLKYSNAEIRSTIAVLKSLEKLSEIEVLHMSVREQYF
FFQQIGKNFPALAVVAVAKGISMEEINCLIERYINPDDLVAHPTTLLSGNDLITALKLPKGPIVGQLLTEIQIARAEENI
LTQDEAIKLAQKLIAFRNDPSSF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=250, Percent_Identity=30, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789436, Length=222, Percent_Identity=31.981981981982, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47501; Mature: 47501

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINSLSALSPENWPFEAKMLPQNVYLVGGSVRDALLKRKTTYLDLDFIILDQAVSIARN
CCCCCCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
IAKEYQAGFVVLDAERKIARVVFKGTTVDFAEIEGNSLKEDLWRRDFTINAIAYNPFTNE
HHHHHCCCEEEEECCHHEEHHEEECCCEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHH
LIDLFNALKDLQDGIIRMVKSENLKEDPLRLLRAYRQAAQLGFIISPETQTTIREFAPLI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
TQVATERVQTELGYLLSHPQGMIWIEKAWKNGLLSDYFPAATENLGIGKKVEIVVEKLQK
HHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHC
NWSTLKVELGSGVRNTVKTSLLAIAKLAILVNSNPQKAEVELMRLKYSNAEIRSTIAVLK
CCCEEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCHHHHHHHHHHH
SLEKLSEIEVLHMSVREQYFFFQQIGKNFPALAVVAVAKGISMEEINCLIERYINPDDLV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
AHPTTLLSGNDLITALKLPKGPIVGQLLTEIQIARAEENILTQDEAIKLAQKLIAFRNDP
CCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
SSF
CCC
>Mature Secondary Structure
MLINSLSALSPENWPFEAKMLPQNVYLVGGSVRDALLKRKTTYLDLDFIILDQAVSIARN
CCCCCCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
IAKEYQAGFVVLDAERKIARVVFKGTTVDFAEIEGNSLKEDLWRRDFTINAIAYNPFTNE
HHHHHCCCEEEEECCHHEEHHEEECCCEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHH
LIDLFNALKDLQDGIIRMVKSENLKEDPLRLLRAYRQAAQLGFIISPETQTTIREFAPLI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
TQVATERVQTELGYLLSHPQGMIWIEKAWKNGLLSDYFPAATENLGIGKKVEIVVEKLQK
HHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHC
NWSTLKVELGSGVRNTVKTSLLAIAKLAILVNSNPQKAEVELMRLKYSNAEIRSTIAVLK
CCCEEEEEECCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCHHHHHHHHHHH
SLEKLSEIEVLHMSVREQYFFFQQIGKNFPALAVVAVAKGISMEEINCLIERYINPDDLV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
AHPTTLLSGNDLITALKLPKGPIVGQLLTEIQIARAEENILTQDEAIKLAQKLIAFRNDP
CCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
SSF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA