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The map label for this gene is crp [H]

Identifier: 113474821

GI number: 113474821

Start: 1624555

End: 1625238

Strand: Direct

Name: crp [H]

Synonym: Tery_1037

Alternate gene names: 113474821

Gene position: 1624555-1625238 (Clockwise)

Preceding gene: 113474819

Following gene: 113474822

Centisome position: 20.96

GC content: 38.01

Gene sequence:

>684_bases
ATGCAAACAGAAGCTTTCAGTGAGCTTTTTCCTTTATTTAAAGGAGCTAATCCAGAAACATTGGAATGGCTCTTATCCGT
TGCAGTAAAACATGAGTATCCCTCAAATAGATTAGTCCTAATGGAAGATGCTTGGGGTAATGCTGTTTACTTTGTGGTAT
CGGGATGGGTCAAAGTCAGACGCAGATTGTCCCCTGATAACTCAGTTACATTGGCTATTCTAGGTAGAGGAGATTTTTTT
GGTGAAATGGCCATTCTAGATGAATCCCCCCGCTCAAATGATGTTGTTGCCCTATCTCCGGTTCGTTTGATTAGTATTTC
GGCTCAAAGATTTATTCAAACTCTCTTCAAAGATCCTCAGTTACATCACCGAATGCTTCAACTGATGGTTAAGCGTCTCA
GGCAGACAAACGTTCGTTATCAATTACGTAATAGACCTCCAGCAGTCAAACTTGCTAATACATTAGTAGAATTAGCTGAA
AACTACGGTCAGACCACTGAAAAAACCTGTGAAATATTTAATATTCCTGACCAAGATTTAGGAGATGTAACAGATATTAC
AATTGATGAAACTACTAAAATTATGGCCAAACTACATAATAAGGGTTGGATTAAGAGAGACCCGGAACATCAGTTAATGC
ACTTACTTAATCTTAAACATTTAATGCATTTAGCCAGTCAGTAA

Upstream 100 bases:

>100_bases
ACCAGCATACCTAGGTTTTAAGTCTCGGTAATAGTTTCTCTGACTTTAAGTCAGGTAATATGTGAACACAAAGAAGATAC
CATAAAGAGTGCTAATTTTC

Downstream 100 bases:

>100_bases
ATTAGATTAATAATTTTTTAAATACTCAAGTTGATAATAAAAAGTCAAAAAAATCAGCTATCTTTCTTGAAAAATTTGCA
TTAAAACTATAGCAAATTTG

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MQTEAFSELFPLFKGANPETLEWLLSVAVKHEYPSNRLVLMEDAWGNAVYFVVSGWVKVRRRLSPDNSVTLAILGRGDFF
GEMAILDESPRSNDVVALSPVRLISISAQRFIQTLFKDPQLHHRMLQLMVKRLRQTNVRYQLRNRPPAVKLANTLVELAE
NYGQTTEKTCEIFNIPDQDLGDVTDITIDETTKIMAKLHNKGWIKRDPEHQLMHLLNLKHLMHLASQ

Sequences:

>Translated_227_residues
MQTEAFSELFPLFKGANPETLEWLLSVAVKHEYPSNRLVLMEDAWGNAVYFVVSGWVKVRRRLSPDNSVTLAILGRGDFF
GEMAILDESPRSNDVVALSPVRLISISAQRFIQTLFKDPQLHHRMLQLMVKRLRQTNVRYQLRNRPPAVKLANTLVELAE
NYGQTTEKTCEIFNIPDQDLGDVTDITIDETTKIMAKLHNKGWIKRDPEHQLMHLLNLKHLMHLASQ
>Mature_227_residues
MQTEAFSELFPLFKGANPETLEWLLSVAVKHEYPSNRLVLMEDAWGNAVYFVVSGWVKVRRRLSPDNSVTLAILGRGDFF
GEMAILDESPRSNDVVALSPVRLISISAQRFIQTLFKDPQLHHRMLQLMVKRLRQTNVRYQLRNRPPAVKLANTLVELAE
NYGQTTEKTCEIFNIPDQDLGDVTDITIDETTKIMAKLHNKGWIKRDPEHQLMHLLNLKHLMHLASQ

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=187, Percent_Identity=26.7379679144385, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26100; Mature: 26100

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTEAFSELFPLFKGANPETLEWLLSVAVKHEYPSNRLVLMEDAWGNAVYFVVSGWVKVR
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHH
RRLSPDNSVTLAILGRGDFFGEMAILDESPRSNDVVALSPVRLISISAQRFIQTLFKDPQ
HHCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHH
LHHRMLQLMVKRLRQTNVRYQLRNRPPAVKLANTLVELAENYGQTTEKTCEIFNIPDQDL
HHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCC
GDVTDITIDETTKIMAKLHNKGWIKRDPEHQLMHLLNLKHLMHLASQ
CCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTEAFSELFPLFKGANPETLEWLLSVAVKHEYPSNRLVLMEDAWGNAVYFVVSGWVKVR
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHH
RRLSPDNSVTLAILGRGDFFGEMAILDESPRSNDVVALSPVRLISISAQRFIQTLFKDPQ
HHCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHH
LHHRMLQLMVKRLRQTNVRYQLRNRPPAVKLANTLVELAENYGQTTEKTCEIFNIPDQDL
HHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCC
GDVTDITIDETTKIMAKLHNKGWIKRDPEHQLMHLLNLKHLMHLASQ
CCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]