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The map label for this gene is gldA [C]

Identifier: 113474810

GI number: 113474810

Start: 1594657

End: 1595790

Strand: Direct

Name: gldA [C]

Synonym: Tery_1022

Alternate gene names: 113474810

Gene position: 1594657-1595790 (Clockwise)

Preceding gene: 113474806

Following gene: 113474811

Centisome position: 20.58

GC content: 39.86

Gene sequence:

>1134_bases
ATGATTAAATCTACTCATATCTCCCAGACAGCTACAACCTTACTATCTCTAAACATAGCCCCTACTCAAATTATTCGAGG
TAAAAATGCTCTGGCCCAATCAGGAGAGGCGATCGCTCGTATTGGCCATCATCCCCTAATCGTTGGGAGTCCTCGCTCTT
TGAACATCATCCTGCCCCACCTCCAACCCTTATTACAAATCCATAATTTAAAATTCACCCAAGCTAACTATGGTTCAGAC
TGTAGTGAAACTAGTTTAGCCAATCTTTCTCAAGCTGTAGCCACACAAAAACCAGACCTAATAATTGGTATTGGTGGTGG
TAAAGCTCTTGACACAGCAAAACTCCTAGCTTATAAATGTCATTTGCCCATAATTACCATTCCCACCTCAGCAGCTACTT
GCGCAGCTTGGACAGCATTATCAAATATCTACTCAGACCAAGGGGCCTTCTTATACGATATAAGTTTAGCTAAATGTCCA
GAATTGCTAATTCTAGATTATGGAATTATTAAAACTGGCCCTTCACGGACTTTAGTGGCTGGTATTGGAGATGCGATCGC
CAAATGGTATGAAGCTTCAGTCTCTAGTGGCCATTCAGAGTCAACAATGATTATTTCTGCTGTCCAACAAGCCAGAGTAC
TAAGGGATATCCTTTTTCAAAAATCAACTTTTGCCCTAGAACAACCAGGAGGAGAAACCTGGCAAGAGGTTGTAGATGCA
ACAGTATTATTAGCAGGTTTAATTGGAAGTATTGGTGGTGCACAATGTCGGACTGTGGCAGCCCATGCTATTCATAATGG
CCTCACTCATTTATCTGCTAGCCATGAAAAATTACATGGAGAAAAAGTCGCTTATGGTATATTGGTTCAGCTAAGGTTAG
AAGAAATGCTACAAAGCAATCAACTAGCTGCAACTGCTAGACAACAACTGTTAAAATTCTATAATCAGATTGGTTTACCA
AAAAGCTTAAATGACTTAGGCTTAAAAGAAATTACTTTATCTGAATTACGCCATGTTGCTGAAGTTGCTTGTCATCCAAA
TTCTGACATTCATCGGTTACCATTTAGAATAATTCCAGAGCAATTAGTAGCAGCAATGGTTTCTACAACAGTCCCATTAG
AGCTAGAGAAATAA

Upstream 100 bases:

>100_bases
ATATAGAAAAAAAACTTAAAATTTTTTAAGAAAACCAGACAACCTGAATAATAGTTACCACTTAGTCAATAAAATTTATA
CTAACCTATCCTTGGTCGTT

Downstream 100 bases:

>100_bases
AATTTCAGAACTAACTCATATATTGTTGATATTACTACAACCTCTCAAATGTAGAATGTAGAAATTTTTTATAGTTTTTT
CTTATTCTAGATGTTTAAAA

Product: iron-containing alcohol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIKSTHISQTATTLLSLNIAPTQIIRGKNALAQSGEAIARIGHHPLIVGSPRSLNIILPHLQPLLQIHNLKFTQANYGSD
CSETSLANLSQAVATQKPDLIIGIGGGKALDTAKLLAYKCHLPIITIPTSAATCAAWTALSNIYSDQGAFLYDISLAKCP
ELLILDYGIIKTGPSRTLVAGIGDAIAKWYEASVSSGHSESTMIISAVQQARVLRDILFQKSTFALEQPGGETWQEVVDA
TVLLAGLIGSIGGAQCRTVAAHAIHNGLTHLSASHEKLHGEKVAYGILVQLRLEEMLQSNQLAATARQQLLKFYNQIGLP
KSLNDLGLKEITLSELRHVAEVACHPNSDIHRLPFRIIPEQLVAAMVSTTVPLELEK

Sequences:

>Translated_377_residues
MIKSTHISQTATTLLSLNIAPTQIIRGKNALAQSGEAIARIGHHPLIVGSPRSLNIILPHLQPLLQIHNLKFTQANYGSD
CSETSLANLSQAVATQKPDLIIGIGGGKALDTAKLLAYKCHLPIITIPTSAATCAAWTALSNIYSDQGAFLYDISLAKCP
ELLILDYGIIKTGPSRTLVAGIGDAIAKWYEASVSSGHSESTMIISAVQQARVLRDILFQKSTFALEQPGGETWQEVVDA
TVLLAGLIGSIGGAQCRTVAAHAIHNGLTHLSASHEKLHGEKVAYGILVQLRLEEMLQSNQLAATARQQLLKFYNQIGLP
KSLNDLGLKEITLSELRHVAEVACHPNSDIHRLPFRIIPEQLVAAMVSTTVPLELEK
>Mature_377_residues
MIKSTHISQTATTLLSLNIAPTQIIRGKNALAQSGEAIARIGHHPLIVGSPRSLNIILPHLQPLLQIHNLKFTQANYGSD
CSETSLANLSQAVATQKPDLIIGIGGGKALDTAKLLAYKCHLPIITIPTSAATCAAWTALSNIYSDQGAFLYDISLAKCP
ELLILDYGIIKTGPSRTLVAGIGDAIAKWYEASVSSGHSESTMIISAVQQARVLRDILFQKSTFALEQPGGETWQEVVDA
TVLLAGLIGSIGGAQCRTVAAHAIHNGLTHLSASHEKLHGEKVAYGILVQLRLEEMLQSNQLAATARQQLLKFYNQIGLP
KSLNDLGLKEITLSELRHVAEVACHPNSDIHRLPFRIIPEQLVAAMVSTTVPLELEK

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=360, Percent_Identity=33.6111111111111, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786815, Length=292, Percent_Identity=38.013698630137, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 40490; Mature: 40490

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSTHISQTATTLLSLNIAPTQIIRGKNALAQSGEAIARIGHHPLIVGSPRSLNIILPH
CCCCCCCHHHHHHHHEECCCHHHHHCCCHHHHHCCHHHHHCCCCCEEEECCCCEEEEHHH
LQPLLQIHNLKFTQANYGSDCSETSLANLSQAVATQKPDLIIGIGGGKALDTAKLLAYKC
HHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHEEE
HLPIITIPTSAATCAAWTALSNIYSDQGAFLYDISLAKCPELLILDYGIIKTGPSRTLVA
CCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCEEEEECCCEECCCCCCHHH
GIGDAIAKWYEASVSSGHSESTMIISAVQQARVLRDILFQKSTFALEQPGGETWQEVVDA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHH
TVLLAGLIGSIGGAQCRTVAAHAIHNGLTHLSASHEKLHGEKVAYGILVQLRLEEMLQSN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCC
QLAATARQQLLKFYNQIGLPKSLNDLGLKEITLSELRHVAEVACHPNSDIHRLPFRIIPE
HHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHCHH
QLVAAMVSTTVPLELEK
HHHHHHHHCCCCEEECC
>Mature Secondary Structure
MIKSTHISQTATTLLSLNIAPTQIIRGKNALAQSGEAIARIGHHPLIVGSPRSLNIILPH
CCCCCCCHHHHHHHHEECCCHHHHHCCCHHHHHCCHHHHHCCCCCEEEECCCCEEEEHHH
LQPLLQIHNLKFTQANYGSDCSETSLANLSQAVATQKPDLIIGIGGGKALDTAKLLAYKC
HHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHEEE
HLPIITIPTSAATCAAWTALSNIYSDQGAFLYDISLAKCPELLILDYGIIKTGPSRTLVA
CCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCEEEEECCCEECCCCCCHHH
GIGDAIAKWYEASVSSGHSESTMIISAVQQARVLRDILFQKSTFALEQPGGETWQEVVDA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHH
TVLLAGLIGSIGGAQCRTVAAHAIHNGLTHLSASHEKLHGEKVAYGILVQLRLEEMLQSN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCC
QLAATARQQLLKFYNQIGLPKSLNDLGLKEITLSELRHVAEVACHPNSDIHRLPFRIIPE
HHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHCHH
QLVAAMVSTTVPLELEK
HHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]