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The map label for this gene is dnaK [H]

Identifier: 113474706

GI number: 113474706

Start: 1414773

End: 1416365

Strand: Direct

Name: dnaK [H]

Synonym: Tery_0893

Alternate gene names: 113474706

Gene position: 1414773-1416365 (Clockwise)

Preceding gene: 113474705

Following gene: 113474707

Centisome position: 18.25

GC content: 39.55

Gene sequence:

>1593_bases
ATGACTATAGCTATAGATTTCGGAACAAGTAATACTGTTATTTCTCGTTGGAATCAAACAACTAAACAACCAGAAATTGT
CAAGTTACCAAATATCTCAATTCTTTCTGTGGAAAATCCGCCTTTAATTCCTAGCTTAGTCTATGTGGAAAATGTTGCTG
AAAAAAAAGTAGTAGTAGGTCAACAGGTTAGAGATAAAGGTTACGATGTTAATACGGAATCTAGATTTTTTCGTAATTTC
AAACGGGGTATTGGTGCAAATGTTCAAGGATTTTTGCCGGAACTTGACGGTCAAATTGTTTCTTTTGAACAGGTAGGGAA
ATTGTTTTTAGAAGAAGTTGTAGCAGGTCTTCAGAAAGTGCCTTTGCCAGTAGAGTCTTTAATTTTTACTGTTCCAGTAG
ATAGTTTTGAAGCTTATCGAAATTGGTTGGGGGAAGTTTGTCAGTCTCTAGAGGTTGAACAGGTAAGAATGATTGATGAA
CCTACTGCTGCGGCTTTAGGTTATGGGATGGCAGATGCGGAAATTTTGTTGGTGGTTGATTTTGGTGGGGGAACTTTAGA
TTTATCTTTGGTAAGGTTGGATAATGTGATGGCAAAACAACCTTTGGGTTATATTCTGAAATGGGGTAAAAAATCTTATG
GTAACAATTCAAGTCAAAAGGCAAGAACTGCAAGGGTTTTGGCAAAGGCTGGAGAGAATTTAGGGGGTTCGGATATTGAT
AATTGGTTGGTGGATTATTTTGTTAAGAGTCAAGGGATAGAAAAGAGCTCTTTAGTTACTCGTTTGGCAGAAAAGTTAAA
AATTCAATTGTCTTTGCAGCAAGAGGGAACGGAGGTTTTTTTTGATGATGAAAGTTTGGAAAGTTATGAGCTGGGGTTGG
GGCGGGATGCTTTTGAGGAAATTCTCCGGGAAAATGGTTTTTTTGCTAGTCTTGATCAGTCAATGAGTCGGGTTTTACAG
CAGGCAAAAAGACAAGGAATAGAGTTATCGGATATTGGTGCTGTTTTATTGGTGGGAGGAACGGTGCAAATTCCGGCGGT
TCAGAGGTGGGTAAGGGAATATTTTGATGCTGGAAAAATTCGTTGTGAAAAACCTTTTGAGGCAATTTCTCAGGGAGCGT
TGCAATTAGTTCAAGGAGTGGAGGTTAAGGATTTTTTGTATCACGGTTATGGTATCCGGTATTGGGATAGAAAGAAAAAT
GCTCATGGTTGGCATCCGTTGATTAAGTCTGGTCAACCGTATCCAATGAGTAGTCCTGTAGAGCTGGTTTTGGGTGCTTC
TATGAATAGTCAACCTAGTATAGAGTTGGTTTTGGGAGAGTTGGGTGCTGAGTCTGCTGGTACAGAGGTTTTTTTTGATG
GCGATCGCCTTATTACTCGGAAAGTTTCTGGGGAGCAAACTGCTGTACAACTTTTAAATGATAGAGAGGGAGGTCGTAGT
ATTGCTCAATTAAGTCCTTTGGGAAATCCGGGGAGGGATCGCATTAAGGTTTTGTTTTGGGTGGATGAGTCTAGGTTTTT
GAGAATAACGGTGGAGGATTTATTGACGAATGAAATTTTGTTAAATGAGCAGGCTGTTGTACAGTTGAGTTAG

Upstream 100 bases:

>100_bases
TTCCTTTGGGATTTATCAAAATGATTTTCATTATTTAAGTGAAGCATTTAAAAGATATAAAGAAGGAAGCAATATATCAA
TTTCCATCAATTAAGAACTA

Downstream 100 bases:

>100_bases
GGTAGGAGGCTGTTAGTTAGTGGCTGGAAAAAGAGGTTTTGGAGGGATTTTATTCAATATTATCCTTTTGTTTTAGTATT
ATATTTAACATAATAATTTA

Product: heat shock protein 70

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTIAIDFGTSNTVISRWNQTTKQPEIVKLPNISILSVENPPLIPSLVYVENVAEKKVVVGQQVRDKGYDVNTESRFFRNF
KRGIGANVQGFLPELDGQIVSFEQVGKLFLEEVVAGLQKVPLPVESLIFTVPVDSFEAYRNWLGEVCQSLEVEQVRMIDE
PTAAALGYGMADAEILLVVDFGGGTLDLSLVRLDNVMAKQPLGYILKWGKKSYGNNSSQKARTARVLAKAGENLGGSDID
NWLVDYFVKSQGIEKSSLVTRLAEKLKIQLSLQQEGTEVFFDDESLESYELGLGRDAFEEILRENGFFASLDQSMSRVLQ
QAKRQGIELSDIGAVLLVGGTVQIPAVQRWVREYFDAGKIRCEKPFEAISQGALQLVQGVEVKDFLYHGYGIRYWDRKKN
AHGWHPLIKSGQPYPMSSPVELVLGASMNSQPSIELVLGELGAESAGTEVFFDGDRLITRKVSGEQTAVQLLNDREGGRS
IAQLSPLGNPGRDRIKVLFWVDESRFLRITVEDLLTNEILLNEQAVVQLS

Sequences:

>Translated_530_residues
MTIAIDFGTSNTVISRWNQTTKQPEIVKLPNISILSVENPPLIPSLVYVENVAEKKVVVGQQVRDKGYDVNTESRFFRNF
KRGIGANVQGFLPELDGQIVSFEQVGKLFLEEVVAGLQKVPLPVESLIFTVPVDSFEAYRNWLGEVCQSLEVEQVRMIDE
PTAAALGYGMADAEILLVVDFGGGTLDLSLVRLDNVMAKQPLGYILKWGKKSYGNNSSQKARTARVLAKAGENLGGSDID
NWLVDYFVKSQGIEKSSLVTRLAEKLKIQLSLQQEGTEVFFDDESLESYELGLGRDAFEEILRENGFFASLDQSMSRVLQ
QAKRQGIELSDIGAVLLVGGTVQIPAVQRWVREYFDAGKIRCEKPFEAISQGALQLVQGVEVKDFLYHGYGIRYWDRKKN
AHGWHPLIKSGQPYPMSSPVELVLGASMNSQPSIELVLGELGAESAGTEVFFDGDRLITRKVSGEQTAVQLLNDREGGRS
IAQLSPLGNPGRDRIKVLFWVDESRFLRITVEDLLTNEILLNEQAVVQLS
>Mature_529_residues
TIAIDFGTSNTVISRWNQTTKQPEIVKLPNISILSVENPPLIPSLVYVENVAEKKVVVGQQVRDKGYDVNTESRFFRNFK
RGIGANVQGFLPELDGQIVSFEQVGKLFLEEVVAGLQKVPLPVESLIFTVPVDSFEAYRNWLGEVCQSLEVEQVRMIDEP
TAAALGYGMADAEILLVVDFGGGTLDLSLVRLDNVMAKQPLGYILKWGKKSYGNNSSQKARTARVLAKAGENLGGSDIDN
WLVDYFVKSQGIEKSSLVTRLAEKLKIQLSLQQEGTEVFFDDESLESYELGLGRDAFEEILRENGFFASLDQSMSRVLQQ
AKRQGIELSDIGAVLLVGGTVQIPAVQRWVREYFDAGKIRCEKPFEAISQGALQLVQGVEVKDFLYHGYGIRYWDRKKNA
HGWHPLIKSGQPYPMSSPVELVLGASMNSQPSIELVLGELGAESAGTEVFFDGDRLITRKVSGEQTAVQLLNDREGGRSI
AQLSPLGNPGRDRIKVLFWVDESRFLRITVEDLLTNEILLNEQAVVQLS

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI16507237, Length=268, Percent_Identity=29.1044776119403, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI24234688, Length=299, Percent_Identity=26.7558528428094, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786196, Length=429, Percent_Identity=29.3706293706294, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17534771, Length=318, Percent_Identity=28.3018867924528, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17568549, Length=277, Percent_Identity=30.6859205776173, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI32564617, Length=277, Percent_Identity=27.0758122743682, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71984444, Length=332, Percent_Identity=25.9036144578313, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322505, Length=430, Percent_Identity=27.906976744186, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322426, Length=412, Percent_Identity=25.2427184466019, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641406, Length=269, Percent_Identity=29.7397769516729, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641408, Length=269, Percent_Identity=29.7397769516729, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641402, Length=269, Percent_Identity=29.7397769516729, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641404, Length=269, Percent_Identity=29.7397769516729, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 58836; Mature: 58705

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAIDFGTSNTVISRWNQTTKQPEIVKLPNISILSVENPPLIPSLVYVENVAEKKVVVG
CEEEEEECCCCHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
QQVRDKGYDVNTESRFFRNFKRGIGANVQGFLPELDGQIVSFEQVGKLFLEEVVAGLQKV
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHC
PLPVESLIFTVPVDSFEAYRNWLGEVCQSLEVEQVRMIDEPTAAALGYGMADAEILLVVD
CCCHHHEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCCCCCCEEEEEEE
FGGGTLDLSLVRLDNVMAKQPLGYILKWGKKSYGNNSSQKARTARVLAKAGENLGGSDID
CCCCEEEEEEEHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
NWLVDYFVKSQGIEKSSLVTRLAEKLKIQLSLQQEGTEVFFDDESLESYELGLGRDAFEE
HHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCCCEEEECCCCCCHHHCCCCHHHHHH
ILRENGFFASLDQSMSRVLQQAKRQGIELSDIGAVLLVGGTVQIPAVQRWVREYFDAGKI
HHHCCCCEEHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCEEECHHHHHHHHHHHCCCCE
RCEKPFEAISQGALQLVQGVEVKDFLYHGYGIRYWDRKKNAHGWHPLIKSGQPYPMSSPV
EECCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCCCCCCCCH
ELVLGASMNSQPSIELVLGELGAESAGTEVFFDGDRLITRKVSGEQTAVQLLNDREGGRS
HEEEECCCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHCCCCCCC
IAQLSPLGNPGRDRIKVLFWVDESRFLRITVEDLLTNEILLNEQAVVQLS
HHHCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TIAIDFGTSNTVISRWNQTTKQPEIVKLPNISILSVENPPLIPSLVYVENVAEKKVVVG
EEEEEECCCCHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
QQVRDKGYDVNTESRFFRNFKRGIGANVQGFLPELDGQIVSFEQVGKLFLEEVVAGLQKV
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHC
PLPVESLIFTVPVDSFEAYRNWLGEVCQSLEVEQVRMIDEPTAAALGYGMADAEILLVVD
CCCHHHEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCCCCCCEEEEEEE
FGGGTLDLSLVRLDNVMAKQPLGYILKWGKKSYGNNSSQKARTARVLAKAGENLGGSDID
CCCCEEEEEEEHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
NWLVDYFVKSQGIEKSSLVTRLAEKLKIQLSLQQEGTEVFFDDESLESYELGLGRDAFEE
HHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCCCEEEECCCCCCHHHCCCCHHHHHH
ILRENGFFASLDQSMSRVLQQAKRQGIELSDIGAVLLVGGTVQIPAVQRWVREYFDAGKI
HHHCCCCEEHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCEEECHHHHHHHHHHHCCCCE
RCEKPFEAISQGALQLVQGVEVKDFLYHGYGIRYWDRKKNAHGWHPLIKSGQPYPMSSPV
EECCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCCCCCCCCH
ELVLGASMNSQPSIELVLGELGAESAGTEVFFDGDRLITRKVSGEQTAVQLLNDREGGRS
HEEEECCCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHCCCCCCC
IAQLSPLGNPGRDRIKVLFWVDESRFLRITVEDLLTNEILLNEQAVVQLS
HHHCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA