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The map label for this gene is rne [H]

Identifier: 113474351

GI number: 113474351

Start: 772170

End: 774218

Strand: Reverse

Name: rne [H]

Synonym: Tery_0483

Alternate gene names: 113474351

Gene position: 774218-772170 (Counterclockwise)

Preceding gene: 113474352

Following gene: 113474350

Centisome position: 9.99

GC content: 40.31

Gene sequence:

>2049_bases
ATGCCGAAGCAAATAATTATCGCTGAACAGCACAGAATTGCCGCCGTATTTTCTGAAGACCAAATACAAGAACTTGTTGT
TGCTAAAGGTAGCCATCAAGTTAGTGATATCTATCTCGGAACCGTAGAAAACGTAATACCTGGAATAGATGCAGCTTTTG
TCAATATTGGAGATCCTGAGCGCAATGGATTTATTCATGTCAGTGATTTAGGCCCATTGCGATTGAAACGATCCTCTGGT
GCAATTACAGAACTACTTACACCCCAACAAAAAGTCTTAGTGCAAGTGATGAAAGAACCCACTGGTACAAAAGGACCACG
TTTAACAGGTAATATAACCCTACCAGGTCGTTATTTAGTTTTGATGCCTTATGGAAGGGGTGTAAATCTATCAAGGCGTA
TTAGTTCTGAGAGTGAACGAAATCGTCTTAGAGCGTTGGCAATTTTAATCAAACCAGCAGGTATGGGCCTGTTAGTTCGT
ACTGAAGCAGAAGGTATGGAAGAAGAGGCAATTCTGGAAGATTTAGAGGTTTTGCAGCAACAATGGGAAATAATCCAAAT
TGAAGCTAATTCTAACAGAGCTCCTGCACTACTAAATCGTGATAATGATTTTATTCAACGGGTACTGCGGGATATGTATA
GTACTGATGTTAATCGAATTGTAGTGGACTATAGTGAGGGAGTGAAGCGAGTCAAGCAACATTTGCTCAACTGGAGTGGG
GGTAAACCATTGCAAGTTTTAATTGACCATCATGGCGATCGCATCCCTATTCTAGAATACTTCCGGGTGAATGCTGCGAT
TAGAGAAGCTCTCAGACCAAGAGTAGATTTACCTTCTGGTGGTTATGTTATTATCCAACCAACTGAAGCACTAACTGTTA
TAGATGTCAACTCTGGTTCTTTTACACGGTCTGCTACAGCTCGGGAAACTGTACTTTGGACAAATTGTGAAGCGGCTACG
GAAATAGCCCGTCAACTACGCCTACGTAACATTGCAGGAGTTATTATTGTTGATTTTATCGACATGGACTCACGACGAGA
CCAACTGCAAGTTCTGGAACATTTTAATCAAGTTCTCAGAGCTGATAAAGCTAGGCCTCAGATAGCTCAGTTGTCAGAAT
TGGGTTTAGTCGAATTGACTCGCAAACGCCAAGGTCAAAATATATATGAGCTATTTGGTCGTAATTGTCCTAGTTGTAAT
GGATTAGGACATTTAGTTCATTTACCTGGTGAATCTGAAGTATCTATTGGGGATGTTAATTTGTCTGTGGTTAATCGAAA
TGTAGATGCTGAGGAATTAACTAAACCTGTAGCTGTTACTCCTCCAACTTTGGTGGGTGTGGCTCAAAACTATTCTTGGG
AGACTAGACAAGGATATCAAGAAGGGGAGAATGCTAATAATGCTTCTGATTTGGAATTGTTGAACCATCCTAGTTACCAG
GAAACTCGTCGTCGTCGTCGTCGTCGAGTAGGGGTTGGTGAAACAATGCAGGATGAGAGTTTGGAATTGCATGAAAGAGA
ATTAGGATCGGGAATGGAGTATCCTATCCTTTCGGAGGGATATTCTCTGACAAGTAATACGAGACGTTCAGGAAGAGAGA
TAATTAGACCCATTACTGAAGCTCCAGAAATAGTAACGGTAGAAATGACTTTTGATGAACAAGATGTATATGCTTTGATG
GGTATTTCTCCATTAACACTTGTAGAGGAGGGAATGGTAAAAAATCCTAAATCAGTCATTATCTCTATTAAACAACCAGG
GGAAGGAGAGAATCATTTGAGCAGAACAAGGGTAGATAAGGAAGAGGTTGAAGTTGAATCAGTTGCAGACTATGAGGAAA
ATGATGATGATCAGGAATTAGATAATTTTGATTTATCTGTTTCTTCTGCTCAAGATGATGATTTTGATGTTCGTGAGGAA
AATGGTAACTATGATTTAGATTTGAGTGATTCAGATGTTGATTTGAATTCTGATAGTAATGACTCTGATGATTCTGGGCC
AATTCGTCGCCGTCGTCGTCGTTCTTCGGCAGTTACAGCGGAAGAATAA

Upstream 100 bases:

>100_bases
GCTAGGAATTCCGTAGTTGTAGGCCGGGGAGAATGTCAAAACGCCACATTTTTATAAAAGGGAAAGACTTGGTAAGCAAG
TCTGCACAGGAGGAAATTGA

Downstream 100 bases:

>100_bases
GCATAAAGTTTGGCTTTTGGACAAGATTATTAAGAGTTGAGAGTGTGGAGTATGGCTGAAGTGAGAAGTTGGGTATCAGT
AACTCAGACATCAGAGGTAG

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MPKQIIIAEQHRIAAVFSEDQIQELVVAKGSHQVSDIYLGTVENVIPGIDAAFVNIGDPERNGFIHVSDLGPLRLKRSSG
AITELLTPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYLVLMPYGRGVNLSRRISSESERNRLRALAILIKPAGMGLLVR
TEAEGMEEEAILEDLEVLQQQWEIIQIEANSNRAPALLNRDNDFIQRVLRDMYSTDVNRIVVDYSEGVKRVKQHLLNWSG
GKPLQVLIDHHGDRIPILEYFRVNAAIREALRPRVDLPSGGYVIIQPTEALTVIDVNSGSFTRSATARETVLWTNCEAAT
EIARQLRLRNIAGVIIVDFIDMDSRRDQLQVLEHFNQVLRADKARPQIAQLSELGLVELTRKRQGQNIYELFGRNCPSCN
GLGHLVHLPGESEVSIGDVNLSVVNRNVDAEELTKPVAVTPPTLVGVAQNYSWETRQGYQEGENANNASDLELLNHPSYQ
ETRRRRRRRVGVGETMQDESLELHERELGSGMEYPILSEGYSLTSNTRRSGREIIRPITEAPEIVTVEMTFDEQDVYALM
GISPLTLVEEGMVKNPKSVIISIKQPGEGENHLSRTRVDKEEVEVESVADYEENDDDQELDNFDLSVSSAQDDDFDVREE
NGNYDLDLSDSDVDLNSDSNDSDDSGPIRRRRRRSSAVTAEE

Sequences:

>Translated_682_residues
MPKQIIIAEQHRIAAVFSEDQIQELVVAKGSHQVSDIYLGTVENVIPGIDAAFVNIGDPERNGFIHVSDLGPLRLKRSSG
AITELLTPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYLVLMPYGRGVNLSRRISSESERNRLRALAILIKPAGMGLLVR
TEAEGMEEEAILEDLEVLQQQWEIIQIEANSNRAPALLNRDNDFIQRVLRDMYSTDVNRIVVDYSEGVKRVKQHLLNWSG
GKPLQVLIDHHGDRIPILEYFRVNAAIREALRPRVDLPSGGYVIIQPTEALTVIDVNSGSFTRSATARETVLWTNCEAAT
EIARQLRLRNIAGVIIVDFIDMDSRRDQLQVLEHFNQVLRADKARPQIAQLSELGLVELTRKRQGQNIYELFGRNCPSCN
GLGHLVHLPGESEVSIGDVNLSVVNRNVDAEELTKPVAVTPPTLVGVAQNYSWETRQGYQEGENANNASDLELLNHPSYQ
ETRRRRRRRVGVGETMQDESLELHERELGSGMEYPILSEGYSLTSNTRRSGREIIRPITEAPEIVTVEMTFDEQDVYALM
GISPLTLVEEGMVKNPKSVIISIKQPGEGENHLSRTRVDKEEVEVESVADYEENDDDQELDNFDLSVSSAQDDDFDVREE
NGNYDLDLSDSDVDLNSDSNDSDDSGPIRRRRRRSSAVTAEE
>Mature_681_residues
PKQIIIAEQHRIAAVFSEDQIQELVVAKGSHQVSDIYLGTVENVIPGIDAAFVNIGDPERNGFIHVSDLGPLRLKRSSGA
ITELLTPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYLVLMPYGRGVNLSRRISSESERNRLRALAILIKPAGMGLLVRT
EAEGMEEEAILEDLEVLQQQWEIIQIEANSNRAPALLNRDNDFIQRVLRDMYSTDVNRIVVDYSEGVKRVKQHLLNWSGG
KPLQVLIDHHGDRIPILEYFRVNAAIREALRPRVDLPSGGYVIIQPTEALTVIDVNSGSFTRSATARETVLWTNCEAATE
IARQLRLRNIAGVIIVDFIDMDSRRDQLQVLEHFNQVLRADKARPQIAQLSELGLVELTRKRQGQNIYELFGRNCPSCNG
LGHLVHLPGESEVSIGDVNLSVVNRNVDAEELTKPVAVTPPTLVGVAQNYSWETRQGYQEGENANNASDLELLNHPSYQE
TRRRRRRRVGVGETMQDESLELHERELGSGMEYPILSEGYSLTSNTRRSGREIIRPITEAPEIVTVEMTFDEQDVYALMG
ISPLTLVEEGMVKNPKSVIISIKQPGEGENHLSRTRVDKEEVEVESVADYEENDDDQELDNFDLSVSSAQDDDFDVREEN
GNYDLDLSDSDVDLNSDSNDSDDSGPIRRRRRRSSAVTAEE

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=376, Percent_Identity=38.2978723404255, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI87082235, Length=387, Percent_Identity=37.7260981912145, Blast_Score=221, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 76435; Mature: 76304

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIIIAEQHRIAAVFSEDQIQELVVAKGSHQVSDIYLGTVENVIPGIDAAFVNIGDPE
CCCCEEEECCCEEEEEECHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCCEEEEECCCCC
RNGFIHVSDLGPLRLKRSSGAITELLTPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYLV
CCCEEEEECCCCEEEECCCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPYGRGVNLSRRISSESERNRLRALAILIKPAGMGLLVRTEAEGMEEEAILEDLEVLQQ
EEECCCCCCHHHHHCCHHHHHHHEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
QWEIIQIEANSNRAPALLNRDNDFIQRVLRDMYSTDVNRIVVDYSEGVKRVKQHLLNWSG
CCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCC
GKPLQVLIDHHGDRIPILEYFRVNAAIREALRPRVDLPSGGYVIIQPTEALTVIDVNSGS
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCC
FTRSATARETVLWTNCEAATEIARQLRLRNIAGVIIVDFIDMDSRRDQLQVLEHFNQVLR
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
ADKARPQIAQLSELGLVELTRKRQGQNIYELFGRNCPSCNGLGHLVHLPGESEVSIGDVN
HCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEE
LSVVNRNVDAEELTKPVAVTPPTLVGVAQNYSWETRQGYQEGENANNASDLELLNHPSYQ
EEEEECCCCHHHHCCCCCCCCCHHEEHHCCCCCHHHCCHHCCCCCCCCCHHHHHCCCCHH
ETRRRRRRRVGVGETMQDESLELHERELGSGMEYPILSEGYSLTSNTRRSGREIIRPITE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
APEIVTVEMTFDEQDVYALMGISPLTLVEEGMVKNPKSVIISIKQPGEGENHLSRTRVDK
CCCEEEEEEEECCCCEEEEECCCHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCH
EEVEVESVADYEENDDDQELDNFDLSVSSAQDDDFDVREENGNYDLDLSDSDVDLNSDSN
HHHHHHHHHCCCCCCCCHHHCCCCEEECCCCCCCCCEEECCCCEEEECCCCCCCCCCCCC
DSDDSGPIRRRRRRSSAVTAEE
CCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PKQIIIAEQHRIAAVFSEDQIQELVVAKGSHQVSDIYLGTVENVIPGIDAAFVNIGDPE
CCCEEEECCCEEEEEECHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCCEEEEECCCCC
RNGFIHVSDLGPLRLKRSSGAITELLTPQQKVLVQVMKEPTGTKGPRLTGNITLPGRYLV
CCCEEEEECCCCEEEECCCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPYGRGVNLSRRISSESERNRLRALAILIKPAGMGLLVRTEAEGMEEEAILEDLEVLQQ
EEECCCCCCHHHHHCCHHHHHHHEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
QWEIIQIEANSNRAPALLNRDNDFIQRVLRDMYSTDVNRIVVDYSEGVKRVKQHLLNWSG
CCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCC
GKPLQVLIDHHGDRIPILEYFRVNAAIREALRPRVDLPSGGYVIIQPTEALTVIDVNSGS
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCC
FTRSATARETVLWTNCEAATEIARQLRLRNIAGVIIVDFIDMDSRRDQLQVLEHFNQVLR
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
ADKARPQIAQLSELGLVELTRKRQGQNIYELFGRNCPSCNGLGHLVHLPGESEVSIGDVN
HCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEE
LSVVNRNVDAEELTKPVAVTPPTLVGVAQNYSWETRQGYQEGENANNASDLELLNHPSYQ
EEEEECCCCHHHHCCCCCCCCCHHEEHHCCCCCHHHCCHHCCCCCCCCCHHHHHCCCCHH
ETRRRRRRRVGVGETMQDESLELHERELGSGMEYPILSEGYSLTSNTRRSGREIIRPITE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
APEIVTVEMTFDEQDVYALMGISPLTLVEEGMVKNPKSVIISIKQPGEGENHLSRTRVDK
CCCEEEEEEEECCCCEEEEECCCHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCH
EEVEVESVADYEENDDDQELDNFDLSVSSAQDDDFDVREENGNYDLDLSDSDVDLNSDSN
HHHHHHHHHCCCCCCCCHHHCCCCEEECCCCCCCCCEEECCCCEEEECCCCCCCCCCCCC
DSDDSGPIRRRRRRSSAVTAEE
CCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]