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The map label for this gene is atfA1 [H]

Identifier: 110835603

GI number: 110835603

Start: 3102693

End: 3104066

Strand: Direct

Name: atfA1 [H]

Synonym: ABO_2742

Alternate gene names: 110835603

Gene position: 3102693-3104066 (Clockwise)

Preceding gene: 110835601

Following gene: 110835604

Centisome position: 99.44

GC content: 55.31

Gene sequence:

>1374_bases
ATGAAAGCGCTTAGCCCAGTGGATCAACTGTTCCTGTGGCTGGAAAAACGACAGCAACCCATGCACGTAGGCGGTTTGCA
GCTGTTTTCCTTCCCGGAAGGTGCCGGCCCCAAGTATGTGAGTGAGCTGGCCCAGCAAATGCGGGATTACTGCCACCCAG
TGGCGCCATTCAACCAGCGCCTGACCCGTCGACTCGGCCAGTATTACTGGACTAGAGACAAACAGTTCGATATCGACCAC
CACTTCCGCCACGAAGCACTCCCCAAACCCGGTCGCATTCGCGAACTGCTTTCTTTGGTCTCCGCCGAACATTCCAACCT
GCTGGACCGGGAGCGCCCCATGTGGGAAGCCCATTTGATCGAAGGGATCCGCGGTCGCCAGTTCGCTCTCTATTATAAGA
TCCACCATTCGGTGATGGATGGCATATCCGCCATGCGTATCGCCTCCAAAACGCTTTCCACTGACCCCAGTGAACGTGAA
ATGGCTCCGGCTTGGGCGTTCAACACCAAAAAACGCTCCCGCTCACTGCCCAGCAACCCGGTTGACATGGCCTCCAGCAT
GGCGCGCCTAACCGCGAGCATAAGCAAACAAGCTGCCACAGTGCCCGGTCTCGCGCGGGAGGTTTACAAAGTCACCCAAA
AAGCCAAAAAAGATGAAAACTATGTGTCTATTTTTCAGGCTCCCGACACGATTCTGAATAATACCATCACCGGTTCACGC
CGCTTTGCCGCCCAGAGCTTTCCATTACCGCGCCTGAAAGTTATCGCCAAGGCCTATAACTGCACCATTAACACCGTGGT
GCTCTCCATGTGTGGCCACGCTCTGCGCGAATACTTGATTAGCCAACACGCGCTGCCCGATGAGCCACTGATTGCCATGG
TGCCCATGAGCCTGCGGCAGGACGACAGCACTGGCGGCAACCAGATCGGTATGATCTTGGCTAACCTGGGCACCCACATC
TGTGATCCAGCTAATCGCCTGCGCGTCATCCACGATTCCGTCGAGGAAGCCAAATCCCGCTTCTCGCAGATGAGCCCGGA
AGAAATTCTCAATTTCACCGCCCTCACCATGGCTCCCACCGGCTTGAACTTACTGACCGGCCTAGCGCCAAAATGGCGGG
CCTTCAACGTGGTGATTTCCAACATACCCGGGCCGAAAGAGCCGCTGTACTGGAATGGTGCACAGCTGCAAGGAGTGTAT
CCAGTATCCATTGCCTTGGATCGCATCGCCCTAAATATCACCCTCACCAGTTATGTAGACCAGATGGAATTTGGGCTTAT
CGCCTGCCGCCGTACTCTGCCTTCCATGCAGCGACTACTGGATTACCTGGAACAGTCCATCCGCGAATTGGAAATCGGTG
CAGGAATTAAATAG

Upstream 100 bases:

>100_bases
TTACTATGATTTACTCTTGCGCAGCTGGCATGGAGAGTGCATAACCTGTTAAAGGTAGACAGCGTGTGTCTAGGGAGAAA
ATTATTACCAAGGAGAATAT

Downstream 100 bases:

>100_bases
TCACACCAGGCTGTTGATTCCTGACCGGTCCGACGTTTGGCATCCGGCGAACAATTATGGCAACTGAACTAAACCGCATC
AGGCGAGGCGGTCTCAACTG

Product: acyltransferase

Products: NA

Alternate protein names: Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKALSPVDQLFLWLEKRQQPMHVGGLQLFSFPEGAGPKYVSELAQQMRDYCHPVAPFNQRLTRRLGQYYWTRDKQFDIDH
HFRHEALPKPGRIRELLSLVSAEHSNLLDRERPMWEAHLIEGIRGRQFALYYKIHHSVMDGISAMRIASKTLSTDPSERE
MAPAWAFNTKKRSRSLPSNPVDMASSMARLTASISKQAATVPGLAREVYKVTQKAKKDENYVSIFQAPDTILNNTITGSR
RFAAQSFPLPRLKVIAKAYNCTINTVVLSMCGHALREYLISQHALPDEPLIAMVPMSLRQDDSTGGNQIGMILANLGTHI
CDPANRLRVIHDSVEEAKSRFSQMSPEEILNFTALTMAPTGLNLLTGLAPKWRAFNVVISNIPGPKEPLYWNGAQLQGVY
PVSIALDRIALNITLTSYVDQMEFGLIACRRTLPSMQRLLDYLEQSIRELEIGAGIK

Sequences:

>Translated_457_residues
MKALSPVDQLFLWLEKRQQPMHVGGLQLFSFPEGAGPKYVSELAQQMRDYCHPVAPFNQRLTRRLGQYYWTRDKQFDIDH
HFRHEALPKPGRIRELLSLVSAEHSNLLDRERPMWEAHLIEGIRGRQFALYYKIHHSVMDGISAMRIASKTLSTDPSERE
MAPAWAFNTKKRSRSLPSNPVDMASSMARLTASISKQAATVPGLAREVYKVTQKAKKDENYVSIFQAPDTILNNTITGSR
RFAAQSFPLPRLKVIAKAYNCTINTVVLSMCGHALREYLISQHALPDEPLIAMVPMSLRQDDSTGGNQIGMILANLGTHI
CDPANRLRVIHDSVEEAKSRFSQMSPEEILNFTALTMAPTGLNLLTGLAPKWRAFNVVISNIPGPKEPLYWNGAQLQGVY
PVSIALDRIALNITLTSYVDQMEFGLIACRRTLPSMQRLLDYLEQSIRELEIGAGIK
>Mature_457_residues
MKALSPVDQLFLWLEKRQQPMHVGGLQLFSFPEGAGPKYVSELAQQMRDYCHPVAPFNQRLTRRLGQYYWTRDKQFDIDH
HFRHEALPKPGRIRELLSLVSAEHSNLLDRERPMWEAHLIEGIRGRQFALYYKIHHSVMDGISAMRIASKTLSTDPSERE
MAPAWAFNTKKRSRSLPSNPVDMASSMARLTASISKQAATVPGLAREVYKVTQKAKKDENYVSIFQAPDTILNNTITGSR
RFAAQSFPLPRLKVIAKAYNCTINTVVLSMCGHALREYLISQHALPDEPLIAMVPMSLRQDDSTGGNQIGMILANLGTHI
CDPANRLRVIHDSVEEAKSRFSQMSPEEILNFTALTMAPTGLNLLTGLAPKWRAFNVVISNIPGPKEPLYWNGAQLQGVY
PVSIALDRIALNITLTSYVDQMEFGLIACRRTLPSMQRLLDYLEQSIRELEIGAGIK

Specific function: Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS us

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20; =2.3.1.75 [H]

Molecular weight: Translated: 51495; Mature: 51495

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALSPVDQLFLWLEKRQQPMHVGGLQLFSFPEGAGPKYVSELAQQMRDYCHPVAPFNQR
CCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
LTRRLGQYYWTRDKQFDIDHHFRHEALPKPGRIRELLSLVSAEHSNLLDRERPMWEAHLI
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EGIRGRQFALYYKIHHSVMDGISAMRIASKTLSTDPSEREMAPAWAFNTKKRSRSLPSNP
HCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHCCCCHHHHCCCCCCH
VDMASSMARLTASISKQAATVPGLAREVYKVTQKAKKDENYVSIFQAPDTILNNTITGSR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCCH
RFAAQSFPLPRLKVIAKAYNCTINTVVLSMCGHALREYLISQHALPDEPLIAMVPMSLRQ
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHCC
DDSTGGNQIGMILANLGTHICDPANRLRVIHDSVEEAKSRFSQMSPEEILNFTALTMAPT
CCCCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GLNLLTGLAPKWRAFNVVISNIPGPKEPLYWNGAQLQGVYPVSIALDRIALNITLTSYVD
CHHHHHCCCCCHHEEEEEEECCCCCCCCCEECCCEECCEEHHHHEEEHHEEEEEHHHHHH
QMEFGLIACRRTLPSMQRLLDYLEQSIRELEIGAGIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKALSPVDQLFLWLEKRQQPMHVGGLQLFSFPEGAGPKYVSELAQQMRDYCHPVAPFNQR
CCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
LTRRLGQYYWTRDKQFDIDHHFRHEALPKPGRIRELLSLVSAEHSNLLDRERPMWEAHLI
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EGIRGRQFALYYKIHHSVMDGISAMRIASKTLSTDPSEREMAPAWAFNTKKRSRSLPSNP
HCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHCCCCHHHHCCCCCCH
VDMASSMARLTASISKQAATVPGLAREVYKVTQKAKKDENYVSIFQAPDTILNNTITGSR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCCH
RFAAQSFPLPRLKVIAKAYNCTINTVVLSMCGHALREYLISQHALPDEPLIAMVPMSLRQ
HHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHCC
DDSTGGNQIGMILANLGTHICDPANRLRVIHDSVEEAKSRFSQMSPEEILNFTALTMAPT
CCCCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GLNLLTGLAPKWRAFNVVISNIPGPKEPLYWNGAQLQGVYPVSIALDRIALNITLTSYVD
CHHHHHCCCCCHHEEEEEEECCCCCCCCCEECCCEECCEEHHHHEEEHHEEEEEHHHHHH
QMEFGLIACRRTLPSMQRLLDYLEQSIRELEIGAGIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12502715 [H]