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The map label for this gene is ushA [H]

Identifier: 110835562

GI number: 110835562

Start: 3053775

End: 3055754

Strand: Direct

Name: ushA [H]

Synonym: ABO_2701

Alternate gene names: 110835562

Gene position: 3053775-3055754 (Clockwise)

Preceding gene: 110835561

Following gene: 110835564

Centisome position: 97.87

GC content: 56.72

Gene sequence:

>1980_bases
ATGAGTACTTCACTGCGATCCCTGCTGGTTCTGTCTCTGGCCAGCGGCGCCTTGCTGAGTGGCTGTGGCGGCGATGATAA
CAACACCCCACGGGTGGATCTTCGCCTGATGGAAACCACCGACATCCACGCCAACGTAATGGACTATAACTACTACAGCG
ACAGCGTGGATAACGGCCACGGCCTGGTACGCACCGCCATGCTGGTCAAACAGGCCCGCGAGGAAATCACCTACCCGGGT
AACTCCGCGCTGGTGGATAACGGCGACTTAATCCAGGGCAGCCCTATGGGTGACTGGCGTCAAGCTCAAGGGCTCACCAG
CGGCGAGGTGCATCCGGTCTATAAAGTCATGAATGAAATGGCCTACGACGTGGGTAACTACGGCAACCACGAATTCAACT
ATGGCTTGGACTTCCTCGCCGAGAGCGTAAACGACGCCGCCTTCCCTTACATCAGCGCCAACGTTTTTAGCGCCGACGGC
AGCACCCCCTACTTTGACCAGTACACGCTAATCACCAAGACCGTTTATGATCGCGACGGCAATCCTCATAAGCTGACTCT
CGGTTTTATCGGCTTTCTACCGCCACAGATTATGCAGTGGGACAAGAAGAACCTGGAGGGTAAGGTCAGCACTAAAGACA
TCAAGGCCGTTGCCGAGCTGTTGGTCCCGCAGATGAAAGCGGAGGGCGCGGACATCATCGTCGCCATCCCCCATTCCGGT
ATCAACACCACCGCCTATTCGGAAAACGCCAAGGCAGAAAACAGCAGCTGGTACCTGGCCGATGTGGAGGGTATCGACGC
CATCATGTTCGGCCACAGCCACCTAACCTTTCCTTCCATAGACTTTGCCAAAACCGCCAACGTGGATCTGGAAAAAGGCA
CCATCAAGGGTGTGCCCTCGGTGATGCCCGGCTACTGGGGCAGCCACCTGGGCATCATCGACCTGACTCTGGCACTCAAT
GAAGACGACGAAAGCTGGGCCGTGGTGGACAGCAAGGTAGAAGCCCGAGCCCTAAACCCGGAAAGCGAGGCAGACGCCGC
ACTGACCGCATTGGTCAAACCCGACCACGACGCCACACTGGAGTACATGGACCAAGTGATCGGCGAATCCACCGACGACA
TTTTCAGCTTCCTGGCCGTGGTAAAGGATGATCCTTCCATCCAGATTGTTAGCGATGCCCAGAAGGCCTATGTGGAAGAC
GCCATTGTCGGCACCGAGCTGGATGGCCTGCCGGTGCTGTCTGCTGCGGCGCCGTTCAAAGCCTGCGACCGCAAGGGCGT
CTGCGCCGAGGAAAGCAGTTTCACCGTGGTGCCCGCCGGCGAATTGAAAGTGAAAAACATTGCCGACCTGTACCTGTATA
ACAACCGCCTGATGGCGGTTAAAGTCACCGGTGCCGAACTGGAACAATGGCTGGAGTGTTCCGCCTCGCAGTTCTTCCAG
ATCACCGATACGGCTGACCGTCAGGAGCTGGTGGATTTCACTGGCTTCCCCACCTACAACTTCGATGTGATCGACGGCGT
CACCTACCAAATCGATGTAACCGAACCGGCCCGCTACAACGGCGACTGTGTCAAAGTCAGCGATGGAACACGCATTGTCG
ACCTGCAATTTGAAGGAACCGCCATTGATCCTGACCAGACGTTTCTTATTGCCAGTAACGACTACCGCGCCCACGGCGGT
AAATTCGCCGGCACGGGTGGCGATCATGTGGTGATTGAATCCCCGGATGAAAACCGCCAGGTAGTGGGTAACTACATTCA
GGCCAACAGCCCGGTAACACCCACCGCCGACAATAACTGGTCCTTCGCACCCATCCTGGGTTACACCAGCAATCTGGAAA
TCGTATTCCGGGTCCCCAATACCAACCGCGCAAAGAATTTTGTGGCGGATGCGGTTGCCGGAGCGGGTGGCAGCTTCCTC
GCCACGTTACTGGACGACAGTGGTAACGAAGCCATCTACGAACTGAACCTGCAACCCTGA

Upstream 100 bases:

>100_bases
GAGCCATCTCGCGGTAATGGGTAACTGCCCCTGAAACACCCAAGACATCACCCGCGGCGTTCGCGTCGCCCTTCTTTCAA
CTCAACACACAGGAAACACC

Downstream 100 bases:

>100_bases
GGCATCCCGGCCTGCCCCGCACGGTGGGCCGGGTAATATCGCTGTACCCGAAATAACACCAGCCCCCTTACGGCTCGGCC
AAAGGTTCATCGCGTCGAAT

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSTSLRSLLVLSLASGALLSGCGGDDNNTPRVDLRLMETTDIHANVMDYNYYSDSVDNGHGLVRTAMLVKQAREEITYPG
NSALVDNGDLIQGSPMGDWRQAQGLTSGEVHPVYKVMNEMAYDVGNYGNHEFNYGLDFLAESVNDAAFPYISANVFSADG
STPYFDQYTLITKTVYDRDGNPHKLTLGFIGFLPPQIMQWDKKNLEGKVSTKDIKAVAELLVPQMKAEGADIIVAIPHSG
INTTAYSENAKAENSSWYLADVEGIDAIMFGHSHLTFPSIDFAKTANVDLEKGTIKGVPSVMPGYWGSHLGIIDLTLALN
EDDESWAVVDSKVEARALNPESEADAALTALVKPDHDATLEYMDQVIGESTDDIFSFLAVVKDDPSIQIVSDAQKAYVED
AIVGTELDGLPVLSAAAPFKACDRKGVCAEESSFTVVPAGELKVKNIADLYLYNNRLMAVKVTGAELEQWLECSASQFFQ
ITDTADRQELVDFTGFPTYNFDVIDGVTYQIDVTEPARYNGDCVKVSDGTRIVDLQFEGTAIDPDQTFLIASNDYRAHGG
KFAGTGGDHVVIESPDENRQVVGNYIQANSPVTPTADNNWSFAPILGYTSNLEIVFRVPNTNRAKNFVADAVAGAGGSFL
ATLLDDSGNEAIYELNLQP

Sequences:

>Translated_659_residues
MSTSLRSLLVLSLASGALLSGCGGDDNNTPRVDLRLMETTDIHANVMDYNYYSDSVDNGHGLVRTAMLVKQAREEITYPG
NSALVDNGDLIQGSPMGDWRQAQGLTSGEVHPVYKVMNEMAYDVGNYGNHEFNYGLDFLAESVNDAAFPYISANVFSADG
STPYFDQYTLITKTVYDRDGNPHKLTLGFIGFLPPQIMQWDKKNLEGKVSTKDIKAVAELLVPQMKAEGADIIVAIPHSG
INTTAYSENAKAENSSWYLADVEGIDAIMFGHSHLTFPSIDFAKTANVDLEKGTIKGVPSVMPGYWGSHLGIIDLTLALN
EDDESWAVVDSKVEARALNPESEADAALTALVKPDHDATLEYMDQVIGESTDDIFSFLAVVKDDPSIQIVSDAQKAYVED
AIVGTELDGLPVLSAAAPFKACDRKGVCAEESSFTVVPAGELKVKNIADLYLYNNRLMAVKVTGAELEQWLECSASQFFQ
ITDTADRQELVDFTGFPTYNFDVIDGVTYQIDVTEPARYNGDCVKVSDGTRIVDLQFEGTAIDPDQTFLIASNDYRAHGG
KFAGTGGDHVVIESPDENRQVVGNYIQANSPVTPTADNNWSFAPILGYTSNLEIVFRVPNTNRAKNFVADAVAGAGGSFL
ATLLDDSGNEAIYELNLQP
>Mature_658_residues
STSLRSLLVLSLASGALLSGCGGDDNNTPRVDLRLMETTDIHANVMDYNYYSDSVDNGHGLVRTAMLVKQAREEITYPGN
SALVDNGDLIQGSPMGDWRQAQGLTSGEVHPVYKVMNEMAYDVGNYGNHEFNYGLDFLAESVNDAAFPYISANVFSADGS
TPYFDQYTLITKTVYDRDGNPHKLTLGFIGFLPPQIMQWDKKNLEGKVSTKDIKAVAELLVPQMKAEGADIIVAIPHSGI
NTTAYSENAKAENSSWYLADVEGIDAIMFGHSHLTFPSIDFAKTANVDLEKGTIKGVPSVMPGYWGSHLGIIDLTLALNE
DDESWAVVDSKVEARALNPESEADAALTALVKPDHDATLEYMDQVIGESTDDIFSFLAVVKDDPSIQIVSDAQKAYVEDA
IVGTELDGLPVLSAAAPFKACDRKGVCAEESSFTVVPAGELKVKNIADLYLYNNRLMAVKVTGAELEQWLECSASQFFQI
TDTADRQELVDFTGFPTYNFDVIDGVTYQIDVTEPARYNGDCVKVSDGTRIVDLQFEGTAIDPDQTFLIASNDYRAHGGK
FAGTGGDHVVIESPDENRQVVGNYIQANSPVTPTADNNWSFAPILGYTSNLEIVFRVPNTNRAKNFVADAVAGAGGSFLA
TLLDDSGNEAIYELNLQP

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=656, Percent_Identity=47.7134146341463, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1786687, Length=597, Percent_Identity=19.9329983249581, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 71514; Mature: 71383

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLRSLLVLSLASGALLSGCGGDDNNTPRVDLRLMETTDIHANVMDYNYYSDSVDNGH
CCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCC
GLVRTAMLVKQAREEITYPGNSALVDNGDLIQGSPMGDWRQAQGLTSGEVHPVYKVMNEM
HHHHHHHHHHHHHHHCCCCCCCEEECCCCEEECCCCCCHHHHCCCCCCCCHHHHHHHHHH
AYDVGNYGNHEFNYGLDFLAESVNDAAFPYISANVFSADGSTPYFDQYTLITKTVYDRDG
HHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEEECCCC
NPHKLTLGFIGFLPPQIMQWDKKNLEGKVSTKDIKAVAELLVPQMKAEGADIIVAIPHSG
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
INTTAYSENAKAENSSWYLADVEGIDAIMFGHSHLTFPSIDFAKTANVDLEKGTIKGVPS
CCCCEECCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCC
VMPGYWGSHLGIIDLTLALNEDDESWAVVDSKVEARALNPESEADAALTALVKPDHDATL
CCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCEEECCCCCCCCCEEEEEECCCCCHHH
EYMDQVIGESTDDIFSFLAVVKDDPSIQIVSDAQKAYVEDAIVGTELDGLPVLSAAAPFK
HHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCEEECCCCHH
ACDRKGVCAEESSFTVVPAGELKVKNIADLYLYNNRLMAVKVTGAELEQWLECSASQFFQ
HCCCCCCCCCCCCEEEEECCCEEECCEEEEEEECCEEEEEEECHHHHHHHHHCCCHHEEE
ITDTADRQELVDFTGFPTYNFDVIDGVTYQIDVTEPARYNGDCVKVSDGTRIVDLQFEGT
EECCCCHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCCEEEECCCCEEEEEEECCC
AIDPDQTFLIASNDYRAHGGKFAGTGGDHVVIESPDENRQVVGNYIQANSPVTPTADNNW
EECCCCEEEEECCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHEECCCCCCCCCCCCC
SFAPILGYTSNLEIVFRVPNTNRAKNFVADAVAGAGGSFLATLLDDSGNEAIYELNLQP
EEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure 
STSLRSLLVLSLASGALLSGCGGDDNNTPRVDLRLMETTDIHANVMDYNYYSDSVDNGH
CCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCC
GLVRTAMLVKQAREEITYPGNSALVDNGDLIQGSPMGDWRQAQGLTSGEVHPVYKVMNEM
HHHHHHHHHHHHHHHCCCCCCCEEECCCCEEECCCCCCHHHHCCCCCCCCHHHHHHHHHH
AYDVGNYGNHEFNYGLDFLAESVNDAAFPYISANVFSADGSTPYFDQYTLITKTVYDRDG
HHHHCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEEECCCC
NPHKLTLGFIGFLPPQIMQWDKKNLEGKVSTKDIKAVAELLVPQMKAEGADIIVAIPHSG
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
INTTAYSENAKAENSSWYLADVEGIDAIMFGHSHLTFPSIDFAKTANVDLEKGTIKGVPS
CCCCEECCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCC
VMPGYWGSHLGIIDLTLALNEDDESWAVVDSKVEARALNPESEADAALTALVKPDHDATL
CCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCEEECCCCCCCCCEEEEEECCCCCHHH
EYMDQVIGESTDDIFSFLAVVKDDPSIQIVSDAQKAYVEDAIVGTELDGLPVLSAAAPFK
HHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCEEECCCCHH
ACDRKGVCAEESSFTVVPAGELKVKNIADLYLYNNRLMAVKVTGAELEQWLECSASQFFQ
HCCCCCCCCCCCCEEEEECCCEEECCEEEEEEECCEEEEEEECHHHHHHHHHCCCHHEEE
ITDTADRQELVDFTGFPTYNFDVIDGVTYQIDVTEPARYNGDCVKVSDGTRIVDLQFEGT
EECCCCHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCCEEEECCCCEEEEEEECCC
AIDPDQTFLIASNDYRAHGGKFAGTGGDHVVIESPDENRQVVGNYIQANSPVTPTADNNW
EECCCCEEEEECCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHEECCCCCCCCCCCCC
SFAPILGYTSNLEIVFRVPNTNRAKNFVADAVAGAGGSFLATLLDDSGNEAIYELNLQP
EEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA