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The map label for this gene is estB [H]

Identifier: 110835525

GI number: 110835525

Start: 3011696

End: 3012553

Strand: Reverse

Name: estB [H]

Synonym: ABO_2664

Alternate gene names: 110835525

Gene position: 3012553-3011696 (Counterclockwise)

Preceding gene: 110835527

Following gene: 110835524

Centisome position: 96.55

GC content: 57.58

Gene sequence:

>858_bases
ATGGATCTGATCATTTTTCTGCTGTCGCTGCTAGTGATGAGCACGGTTACCCTGACACTGGCGTTTTATCTGTGCTGGTA
CTACGACCGATGCAGCTTTCCCGAGCAATCTGAGCTGCCCGGTGAACCCCCTTTACGGCTGTTGCCGACGCTACTTGGCA
TGGCCAAGGAGGCCGCCGCCCTGACAGTGCTAGTGCTTAGTTATCCGTTGCGCCTGATCCACGATTCTTCACCGGTACGT
AGCCGCCATCACGGCGAGCCCCCGGTGATTCTGGTCCACGGTTACGGCGGCAACAGTGCCAACTTTTTATTTATGCAGTG
GCGCCTGAAATGGCGCGGCTGGTCCAATGTGTATTCGGTGAGTTATACCCCGCCGCATATCAATGCCCGCAAACTATCCC
AGCAGGTGGTTGATCATGTAGAGCGTATCCTGGCGACCACAGGCGCGGAAAAAGCCCACTTGGTTTGCCACTCCATGGGC
GGCCCGTTAACCCGCTATGCGCTCAAGAATTTAGGGCTGGCTGGCAAGGTAGACCGGGTGATTACCTTGGGTAGCCCGCA
TTATGGAACGCGAGTTGCCGGCCTGTTTCCGCCATTGGGGGCTGCCGCCCAACTGCGCTACCAGAGCCCTTTTATCCGAG
AGCTGGCCACCGAGGCAACCTGCCCAGGGGGCGCGCGGTACTTCTCCATCTTCTCCAACATGGATAATTTTGTGCTGCCG
GTGTCCGCGGCGGTATTGCACGGTGCGGAAGACAATATCCATGTTCCTTATCTGGGGCACTGCTCGCTGCTCTACAGTAG
CCGGGTTATGGATCAAGTGGAGCGTTGTCTTTTGGCCCCGCCCAAGGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
TGTCTTTTCCACGCGCGTTGTCCGACAGTCATACTAGCGACATGGTATCTTCGGTCACGCTGCCCTAGCGTTGGCACAAT
AACAACGGAGCAATGAAGAG

Downstream 100 bases:

>100_bases
ATATTGATCTGCAACATGGTTGCCTTTCCTCTCTTCGCGGATCATGGTGAATATAAAAATGAGAGAGGGAGTTTGATCAT
GAGTGAATATGGCGCAACGG

Product: hypothetical protein

Products: NA

Alternate protein names: Lipase B; Triacylglycerol lipase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDLIIFLLSLLVMSTVTLTLAFYLCWYYDRCSFPEQSELPGEPPLRLLPTLLGMAKEAAALTVLVLSYPLRLIHDSSPVR
SRHHGEPPVILVHGYGGNSANFLFMQWRLKWRGWSNVYSVSYTPPHINARKLSQQVVDHVERILATTGAEKAHLVCHSMG
GPLTRYALKNLGLAGKVDRVITLGSPHYGTRVAGLFPPLGAAAQLRYQSPFIRELATEATCPGGARYFSIFSNMDNFVLP
VSAAVLHGAEDNIHVPYLGHCSLLYSSRVMDQVERCLLAPPKAAE

Sequences:

>Translated_285_residues
MDLIIFLLSLLVMSTVTLTLAFYLCWYYDRCSFPEQSELPGEPPLRLLPTLLGMAKEAAALTVLVLSYPLRLIHDSSPVR
SRHHGEPPVILVHGYGGNSANFLFMQWRLKWRGWSNVYSVSYTPPHINARKLSQQVVDHVERILATTGAEKAHLVCHSMG
GPLTRYALKNLGLAGKVDRVITLGSPHYGTRVAGLFPPLGAAAQLRYQSPFIRELATEATCPGGARYFSIFSNMDNFVLP
VSAAVLHGAEDNIHVPYLGHCSLLYSSRVMDQVERCLLAPPKAAE
>Mature_285_residues
MDLIIFLLSLLVMSTVTLTLAFYLCWYYDRCSFPEQSELPGEPPLRLLPTLLGMAKEAAALTVLVLSYPLRLIHDSSPVR
SRHHGEPPVILVHGYGGNSANFLFMQWRLKWRGWSNVYSVSYTPPHINARKLSQQVVDHVERILATTGAEKAHLVCHSMG
GPLTRYALKNLGLAGKVDRVITLGSPHYGTRVAGLFPPLGAAAQLRYQSPFIRELATEATCPGGARYFSIFSNMDNFVLP
VSAAVLHGAEDNIHVPYLGHCSLLYSSRVMDQVERCLLAPPKAAE

Specific function: An esterase which preferentially hydrolyzes triacylglyceride substrates with short chain fatty acids (less than C10) with the maximum activity towards tricaprylin (C8:0). Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18 [H]

COG id: COG1075

COG function: function code R; Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002918 [H]

Pfam domain/function: PF01674 Lipase_2 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 31511; Mature: 31511

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIIFLLSLLVMSTVTLTLAFYLCWYYDRCSFPEQSELPGEPPLRLLPTLLGMAKEAAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LTVLVLSYPLRLIHDSSPVRSRHHGEPPVILVHGYGGNSANFLFMQWRLKWRGWSNVYSV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEEEE
SYTPPHINARKLSQQVVDHVERILATTGAEKAHLVCHSMGGPLTRYALKNLGLAGKVDRV
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHCCCCCCCCEE
ITLGSPHYGTRVAGLFPPLGAAAQLRYQSPFIRELATEATCPGGARYFSIFSNMDNFVLP
EEECCCCCCCCHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEH
VSAAVLHGAEDNIHVPYLGHCSLLYSSRVMDQVERCLLAPPKAAE
HHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDLIIFLLSLLVMSTVTLTLAFYLCWYYDRCSFPEQSELPGEPPLRLLPTLLGMAKEAAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LTVLVLSYPLRLIHDSSPVRSRHHGEPPVILVHGYGGNSANFLFMQWRLKWRGWSNVYSV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEEEE
SYTPPHINARKLSQQVVDHVERILATTGAEKAHLVCHSMGGPLTRYALKNLGLAGKVDRV
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHCCCCCCCCEE
ITLGSPHYGTRVAGLFPPLGAAAQLRYQSPFIRELATEATCPGGARYFSIFSNMDNFVLP
EEECCCCCCCCHHHCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEH
VSAAVLHGAEDNIHVPYLGHCSLLYSSRVMDQVERCLLAPPKAAE
HHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]