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The map label for this gene is metF [H]

Identifier: 110835480

GI number: 110835480

Start: 2964020

End: 2964847

Strand: Direct

Name: metF [H]

Synonym: ABO_2619

Alternate gene names: 110835480

Gene position: 2964020-2964847 (Clockwise)

Preceding gene: 110835479

Following gene: 110835481

Centisome position: 95.0

GC content: 54.59

Gene sequence:

>828_bases
ATGAAACGAATCAGTTTTGAATTCTTTCCGCCAAAAACCGACCAGGGGAAAGAAAAGCTACTGGCGACGCAGCAAAAACT
GCTGGTCAAGAACCCCGCGTTTTTTTCCTGCACCTTTGGTGCTGGTGGCTCCACCCGTGACAATACCCGCGACACCGTCA
ATAAGTTGCGCGAGCAACACGGCGACACCGCACCGCATCTGAGCTGCATCGGTCAGAGCCGCGAAGAAATCTTGGAGCTG
GTCAACGGCTACAAAGAAGCCGGCGTGCAACGCATCGTTGCCCTGCGTGGCGACTTACCTTCCGGCATGGGTTACGCCCA
GAGCGAGATTAAATACGCCGACGAACTGGTGCAATTGATCCGCCAGGAAACCGGCGATCACTTCACCCTGGAAGTGGCAG
CCTACCCGGAAATGCACCCGCAAGCGCGCAGCTTCGAAGATGATATTGCGCACTTCAAACGCAAAGTAGATGCCGGCGCC
AACAGCGGCCTAACCCAGTATTTCTACAATGCAGATGCCTACGGCTATTATATGGATGCGTTACAAAAGATTGACTGTGA
CGCTCCTGTCTACCCCGGCATCATGCCCATTCTCAACGTGGCCAACCTGTTACGCTTTTCCAATACCTGTGGCGCCGATG
TGCCACGCTGGCTGCAAAGCAAGCTTAACGACATCAAGGATGATGACGCCGCAGTGAAAGCATTCGGTGAAGAAGTGGTT
ACCCGTTTATGCGAGCGACTGCTGGCCATGGGCGCACCGGGCTTGCACTTCTACACCATGAACCAAGCCGGCCCCAACCT
TCGTATTCTCGACAATCTGGGGATGTGA

Upstream 100 bases:

>100_bases
CCCTACAAGCCCGAACATTATAAGTACTAAACACTGGTACGCCCCACGCATCATGCAACACGCGTGGGGCGTGAGGTGTG
CAGCATGAAGCGAGTTCCCA

Downstream 100 bases:

>100_bases
AAAGGCACCGTTAAGCTTCACGCTACAACACGGGCCGGGGTGGCACCATCCGATAACCGCCCTGCCCGTGCAATGCAGCT
GAACATTTAAACGTGAAAAT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKRISFEFFPPKTDQGKEKLLATQQKLLVKNPAFFSCTFGAGGSTRDNTRDTVNKLREQHGDTAPHLSCIGQSREEILEL
VNGYKEAGVQRIVALRGDLPSGMGYAQSEIKYADELVQLIRQETGDHFTLEVAAYPEMHPQARSFEDDIAHFKRKVDAGA
NSGLTQYFYNADAYGYYMDALQKIDCDAPVYPGIMPILNVANLLRFSNTCGADVPRWLQSKLNDIKDDDAAVKAFGEEVV
TRLCERLLAMGAPGLHFYTMNQAGPNLRILDNLGM

Sequences:

>Translated_275_residues
MKRISFEFFPPKTDQGKEKLLATQQKLLVKNPAFFSCTFGAGGSTRDNTRDTVNKLREQHGDTAPHLSCIGQSREEILEL
VNGYKEAGVQRIVALRGDLPSGMGYAQSEIKYADELVQLIRQETGDHFTLEVAAYPEMHPQARSFEDDIAHFKRKVDAGA
NSGLTQYFYNADAYGYYMDALQKIDCDAPVYPGIMPILNVANLLRFSNTCGADVPRWLQSKLNDIKDDDAAVKAFGEEVV
TRLCERLLAMGAPGLHFYTMNQAGPNLRILDNLGM
>Mature_275_residues
MKRISFEFFPPKTDQGKEKLLATQQKLLVKNPAFFSCTFGAGGSTRDNTRDTVNKLREQHGDTAPHLSCIGQSREEILEL
VNGYKEAGVQRIVALRGDLPSGMGYAQSEIKYADELVQLIRQETGDHFTLEVAAYPEMHPQARSFEDDIAHFKRKVDAGA
NSGLTQYFYNADAYGYYMDALQKIDCDAPVYPGIMPILNVANLLRFSNTCGADVPRWLQSKLNDIKDDDAAVKAFGEEVV
TRLCERLLAMGAPGLHFYTMNQAGPNLRILDNLGM

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=36.1702127659575, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1790377, Length=273, Percent_Identity=35.5311355311355, Blast_Score=191, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=33.0935251798561, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=33.0935251798561, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6325234, Length=276, Percent_Identity=34.4202898550725, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321313, Length=280, Percent_Identity=32.5, Blast_Score=145, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30650; Mature: 30650

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRISFEFFPPKTDQGKEKLLATQQKLLVKNPAFFSCTFGAGGSTRDNTRDTVNKLREQH
CCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHC
GDTAPHLSCIGQSREEILELVNGYKEAGVQRIVALRGDLPSGMGYAQSEIKYADELVQLI
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RQETGDHFTLEVAAYPEMHPQARSFEDDIAHFKRKVDAGANSGLTQYFYNADAYGYYMDA
HHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHH
LQKIDCDAPVYPGIMPILNVANLLRFSNTCGADVPRWLQSKLNDIKDDDAAVKAFGEEVV
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
TRLCERLLAMGAPGLHFYTMNQAGPNLRILDNLGM
HHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCC
>Mature Secondary Structure
MKRISFEFFPPKTDQGKEKLLATQQKLLVKNPAFFSCTFGAGGSTRDNTRDTVNKLREQH
CCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHC
GDTAPHLSCIGQSREEILELVNGYKEAGVQRIVALRGDLPSGMGYAQSEIKYADELVQLI
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RQETGDHFTLEVAAYPEMHPQARSFEDDIAHFKRKVDAGANSGLTQYFYNADAYGYYMDA
HHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHH
LQKIDCDAPVYPGIMPILNVANLLRFSNTCGADVPRWLQSKLNDIKDDDAAVKAFGEEVV
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
TRLCERLLAMGAPGLHFYTMNQAGPNLRILDNLGM
HHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]