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The map label for this gene is kefA [H]

Identifier: 110835420

GI number: 110835420

Start: 2900779

End: 2901582

Strand: Direct

Name: kefA [H]

Synonym: ABO_2559

Alternate gene names: 110835420

Gene position: 2900779-2901582 (Clockwise)

Preceding gene: 110835419

Following gene: 110835427

Centisome position: 92.97

GC content: 53.48

Gene sequence:

>804_bases
ATGAATGATGGAAATATAGTCAACGACCTGACTGTCGTAGTGGAATTGTTCAACGTCTACGCATTAATGCTGCTGTTTAT
CGGTGGATTTGTGCTGTGGGCCCTGAACTGGGGCATCAGTAAAGCAAGCCTGAAAATCACCGAGAAAATGCCCACCCGAC
GCTTCCTGATTTTGCAGATCACCACACTGGTCGGCTTTGTACTCTACACCGTGGGCACCATCGCGCTGGTCATCGGCGTA
CTGCAGCCCCCCAAAGAATTCATGCTCGCTGCCGGCGGCTCCATCGCGGTGGCCCTGGGCTTTGCCCTGAAAGACATTGC
CGCCTCACTCGTAGCCGGTTTGCTGTTGTTATTTGATCGCCCCTTCCGGGTGGGTGACCGGGTCAGCTTCGATAACGTTT
ATGGAGAAATCGTTTCCATCGGCCTGCGCACCGTGCGCCTGCAAACCCTGGACGATAATCTGGTCACCATCCCCAACTCG
CGGTTTATTACCGATGTGGTGGCCTCCGGCAATGCCGGCGAATTGGACATGATGGTGGTCACCGATTTCCACGTGGCCTT
GAATGCCGATATCGAACAGGCCAAAGCCATCGTGGAAGAAATTATCGTCACCAGCCGCTACGCCTACCTGAAAAAGCCAG
TCACCTTTGCCATTGAAGAAATTGAAGTGGGTTACCGGTTGGCAATTCGCCTACGCGCCAAAGCCTATGTTCTGGATGTG
CATTACGAGAAAGCATTTCAGAGCGACATCGTGGTGCGGGTATCCAAACGCTTTACAGAAATGCAGGTGGCTCGGCCGGG
ATAA

Upstream 100 bases:

>100_bases
GATGCAGGGTTTCTGCACAAGAGCGATGATGGCCGCTACCGGTTGGTGTCCCTATGGTACCAGCAGGTGATCAGCTACCT
AACACGGAAGAACCTGCTCA

Downstream 100 bases:

>100_bases
TGGCCAATGCCTTATGACCTGTGCTTTTAGCTCTTCTCATTGGCGGGTTTTTCTGCCTCATCGGACAGCACCGCTTCCAG
CTCCTCACGGGGCATGTAGT

Product: hypothetical protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNDGNIVNDLTVVVELFNVYALMLLFIGGFVLWALNWGISKASLKITEKMPTRRFLILQITTLVGFVLYTVGTIALVIGV
LQPPKEFMLAAGGSIAVALGFALKDIAASLVAGLLLLFDRPFRVGDRVSFDNVYGEIVSIGLRTVRLQTLDDNLVTIPNS
RFITDVVASGNAGELDMMVVTDFHVALNADIEQAKAIVEEIIVTSRYAYLKKPVTFAIEEIEVGYRLAIRLRAKAYVLDV
HYEKAFQSDIVVRVSKRFTEMQVARPG

Sequences:

>Translated_267_residues
MNDGNIVNDLTVVVELFNVYALMLLFIGGFVLWALNWGISKASLKITEKMPTRRFLILQITTLVGFVLYTVGTIALVIGV
LQPPKEFMLAAGGSIAVALGFALKDIAASLVAGLLLLFDRPFRVGDRVSFDNVYGEIVSIGLRTVRLQTLDDNLVTIPNS
RFITDVVASGNAGELDMMVVTDFHVALNADIEQAKAIVEEIIVTSRYAYLKKPVTFAIEEIEVGYRLAIRLRAKAYVLDV
HYEKAFQSDIVVRVSKRFTEMQVARPG
>Mature_267_residues
MNDGNIVNDLTVVVELFNVYALMLLFIGGFVLWALNWGISKASLKITEKMPTRRFLILQITTLVGFVLYTVGTIALVIGV
LQPPKEFMLAAGGSIAVALGFALKDIAASLVAGLLLLFDRPFRVGDRVSFDNVYGEIVSIGLRTVRLQTLDDNLVTIPNS
RFITDVVASGNAGELDMMVVTDFHVALNADIEQAKAIVEEIIVTSRYAYLKKPVTFAIEEIEVGYRLAIRLRAKAYVLDV
HYEKAFQSDIVVRVSKRFTEMQVARPG

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=235, Percent_Identity=24.6808510638298, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367355, Length=148, Percent_Identity=29.0540540540541, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 29488; Mature: 29488

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDGNIVNDLTVVVELFNVYALMLLFIGGFVLWALNWGISKASLKITEKMPTRRFLILQI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
TTLVGFVLYTVGTIALVIGVLQPPKEFMLAAGGSIAVALGFALKDIAASLVAGLLLLFDR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PFRVGDRVSFDNVYGEIVSIGLRTVRLQTLDDNLVTIPNSRFITDVVASGNAGELDMMVV
CCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCEEECCCCCCHHHHHCCCCCCCEEEEEE
TDFHVALNADIEQAKAIVEEIIVTSRYAYLKKPVTFAIEEIEVGYRLAIRLRAKAYVLDV
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEEEEEEEEE
HYEKAFQSDIVVRVSKRFTEMQVARPG
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNDGNIVNDLTVVVELFNVYALMLLFIGGFVLWALNWGISKASLKITEKMPTRRFLILQI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
TTLVGFVLYTVGTIALVIGVLQPPKEFMLAAGGSIAVALGFALKDIAASLVAGLLLLFDR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PFRVGDRVSFDNVYGEIVSIGLRTVRLQTLDDNLVTIPNSRFITDVVASGNAGELDMMVV
CCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCEEECCCCCCHHHHHCCCCCCCEEEEEE
TDFHVALNADIEQAKAIVEEIIVTSRYAYLKKPVTFAIEEIEVGYRLAIRLRAKAYVLDV
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEEEEEEEEE
HYEKAFQSDIVVRVSKRFTEMQVARPG
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]