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The map label for this gene is tolC [H]

Identifier: 110835361

GI number: 110835361

Start: 2835232

End: 2836677

Strand: Direct

Name: tolC [H]

Synonym: ABO_2500

Alternate gene names: 110835361

Gene position: 2835232-2836677 (Clockwise)

Preceding gene: 110835352

Following gene: 110835363

Centisome position: 90.87

GC content: 56.78

Gene sequence:

>1446_bases
ATGAAGCGTAGTCCGGCAACGTCCCTAGTTCTTCTTCTTACCTCACTGGCCAGCGCCGTACAGGCCGCTGATCTCACTCA
AGTCGCTAACGCCGCCTGGAGTTACGATGGTACCTGGCGCTCTGCCCGGATGAACTGGAATGCCGACCAAGAGCTGATGG
TACAAGGCCGTGCCGCCCTGTTCCCGAACATCTCCGCCAGCTACGGACGTTACGACAACGACCGTGAAATCGAAGACGCT
CCTCCTCTCGCACCCGACGAGCAAAATTACGGCAGCGAAGTCAGCACCTTCACTCTAACGCAGCCCCTGTTCCGTGTTGA
TGCCTGGTATGGCTATAAAGAAGCACAGTCCAGCACCGATATCGCGCAGGCTAACTTCGAACAAGCTCGCCAAGACTTTG
TGCTGCGCGTCGCCCAAGGCTACTTCGGTGTACTGCGTGCCTGGGATACCTGGGTATCGGCAAAAGCTGAAGAGAAGGCC
ATCGGTCGCCAATTGGAACAGACCCAAGAGCGGTTTGATGTGGGCCTAGTACCGGCCACCGACGTGGAGGAAGCGCAAGC
AACCTACGACCTGACCCAGGTCAACCTGATTGTCGCCCGCCAGCAATACGAGATTGCCCGTGACCAGCTGGAAACACTCA
CCGGCCAAAAATGGGCAACCCTGCTAGAGCTGCGCAATGAACTGCCCATGGAGGGCCCGCAGCCTACGGACATGAACGAC
TGGATTGAAAAAGCCAAAGCACAAAATCCACAAGTGCTGGCCGCGCTCTACAATACCGAGCTAACCGAAAACACGGCCAA
ACGTCAGCTGGGCACCATGCTGCCTCAGGTTCAACTAGTGGGACAATATCAGCATTCCCACCTCTCTCCAGACAGTTCAT
CGTCAACCGGCAGCTTAAGCAGCAGCTCACTCTACAGCAGCAGCTCACTCTACGATACTGAAACCAAACAGATCGGCATT
GAAGTTAGCATGCCTCTGTTCCAGGGGGGCGGCCTGAACAGCCGCCGCAAAGAAGCCTACCTGCGAGCAGATGCGGCAGA
TTACCAATACGATCAAGCCGTACGCGATATCGTCCAACAGACCCGTAGCGCTTACCGCACCGTGGAAGCGGACGCCCTGC
GCATCAAGGCTCGCCAGCAGGCTATCCGCTCCACTCGCTCTGCTCTGGATGCTACCCAGTCCGGTTATGAAGTGGGCACC
CGTAATGTGGTCGACGTGCTCAATGCTCAGCGCGCCCTGTTCGCCGCCGAACGTGATTACGCCAATGCCCGCTACGACTA
CATCATCAACTCGTTGACCCTGAAATCCACCACCGGAGACCTGCAGCAGGGAGACCTCGCTGCGGTGAATAACTGGCTGG
CACCAGAACAACCCCTAGACCTTTACAACCCGGATCTGGACGGTAAGAGTGAAAAAATGGTCCCGGAGAATTCGTCCATC
GATTGA

Upstream 100 bases:

>100_bases
AGCCAAACAACCGCGGAACTGGCCCAAACAGGCAGTGTTAATTTTCTGTACTTGCCAGTACCATAATGACCAACCAGACA
TACAATAAGAGTGCACCAGA

Downstream 100 bases:

>100_bases
CGCTAACCACCTGAGGCAAACACCGCGCCAGAGATTGCCGTCAACAGGATCGTAAAATATAAAAAAACCGCTAGCCGTTA
CCGGACAGCGGTTTTTTTAT

Product: outer membrane protein TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MKRSPATSLVLLLTSLASAVQAADLTQVANAAWSYDGTWRSARMNWNADQELMVQGRAALFPNISASYGRYDNDREIEDA
PPLAPDEQNYGSEVSTFTLTQPLFRVDAWYGYKEAQSSTDIAQANFEQARQDFVLRVAQGYFGVLRAWDTWVSAKAEEKA
IGRQLEQTQERFDVGLVPATDVEEAQATYDLTQVNLIVARQQYEIARDQLETLTGQKWATLLELRNELPMEGPQPTDMND
WIEKAKAQNPQVLAALYNTELTENTAKRQLGTMLPQVQLVGQYQHSHLSPDSSSSTGSLSSSSLYSSSSLYDTETKQIGI
EVSMPLFQGGGLNSRRKEAYLRADAADYQYDQAVRDIVQQTRSAYRTVEADALRIKARQQAIRSTRSALDATQSGYEVGT
RNVVDVLNAQRALFAAERDYANARYDYIINSLTLKSTTGDLQQGDLAAVNNWLAPEQPLDLYNPDLDGKSEKMVPENSSI
D

Sequences:

>Translated_481_residues
MKRSPATSLVLLLTSLASAVQAADLTQVANAAWSYDGTWRSARMNWNADQELMVQGRAALFPNISASYGRYDNDREIEDA
PPLAPDEQNYGSEVSTFTLTQPLFRVDAWYGYKEAQSSTDIAQANFEQARQDFVLRVAQGYFGVLRAWDTWVSAKAEEKA
IGRQLEQTQERFDVGLVPATDVEEAQATYDLTQVNLIVARQQYEIARDQLETLTGQKWATLLELRNELPMEGPQPTDMND
WIEKAKAQNPQVLAALYNTELTENTAKRQLGTMLPQVQLVGQYQHSHLSPDSSSSTGSLSSSSLYSSSSLYDTETKQIGI
EVSMPLFQGGGLNSRRKEAYLRADAADYQYDQAVRDIVQQTRSAYRTVEADALRIKARQQAIRSTRSALDATQSGYEVGT
RNVVDVLNAQRALFAAERDYANARYDYIINSLTLKSTTGDLQQGDLAAVNNWLAPEQPLDLYNPDLDGKSEKMVPENSSI
D
>Mature_481_residues
MKRSPATSLVLLLTSLASAVQAADLTQVANAAWSYDGTWRSARMNWNADQELMVQGRAALFPNISASYGRYDNDREIEDA
PPLAPDEQNYGSEVSTFTLTQPLFRVDAWYGYKEAQSSTDIAQANFEQARQDFVLRVAQGYFGVLRAWDTWVSAKAEEKA
IGRQLEQTQERFDVGLVPATDVEEAQATYDLTQVNLIVARQQYEIARDQLETLTGQKWATLLELRNELPMEGPQPTDMND
WIEKAKAQNPQVLAALYNTELTENTAKRQLGTMLPQVQLVGQYQHSHLSPDSSSSTGSLSSSSLYSSSSLYDTETKQIGI
EVSMPLFQGGGLNSRRKEAYLRADAADYQYDQAVRDIVQQTRSAYRTVEADALRIKARQQAIRSTRSALDATQSGYEVGT
RNVVDVLNAQRALFAAERDYANARYDYIINSLTLKSTTGDLQQGDLAAVNNWLAPEQPLDLYNPDLDGKSEKMVPENSSI
D

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=462, Percent_Identity=32.034632034632, Blast_Score=207, Evalue=9e-55,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53611; Mature: 53611

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSPATSLVLLLTSLASAVQAADLTQVANAAWSYDGTWRSARMNWNADQELMVQGRAAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHCCCHHH
FPNISASYGRYDNDREIEDAPPLAPDEQNYGSEVSTFTLTQPLFRVDAWYGYKEAQSSTD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHCCCCH
IAQANFEQARQDFVLRVAQGYFGVLRAWDTWVSAKAEEKAIGRQLEQTQERFDVGLVPAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
DVEEAQATYDLTQVNLIVARQQYEIARDQLETLTGQKWATLLELRNELPMEGPQPTDMND
CHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHH
WIEKAKAQNPQVLAALYNTELTENTAKRQLGTMLPQVQLVGQYQHSHLSPDSSSSTGSLS
HHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SSSLYSSSSLYDTETKQIGIEVSMPLFQGGGLNSRRKEAYLRADAADYQYDQAVRDIVQQ
CCHHCCCCCCCCCHHHHEEEEEECCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
TRSAYRTVEADALRIKARQQAIRSTRSALDATQSGYEVGTRNVVDVLNAQRALFAAERDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ANARYDYIINSLTLKSTTGDLQQGDLAAVNNWLAPEQPLDLYNPDLDGKSEKMVPENSSI
CCCHHHHHHHHEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
D
C
>Mature Secondary Structure
MKRSPATSLVLLLTSLASAVQAADLTQVANAAWSYDGTWRSARMNWNADQELMVQGRAAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHCCCHHH
FPNISASYGRYDNDREIEDAPPLAPDEQNYGSEVSTFTLTQPLFRVDAWYGYKEAQSSTD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCHHHHCCCCH
IAQANFEQARQDFVLRVAQGYFGVLRAWDTWVSAKAEEKAIGRQLEQTQERFDVGLVPAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
DVEEAQATYDLTQVNLIVARQQYEIARDQLETLTGQKWATLLELRNELPMEGPQPTDMND
CHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHH
WIEKAKAQNPQVLAALYNTELTENTAKRQLGTMLPQVQLVGQYQHSHLSPDSSSSTGSLS
HHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SSSLYSSSSLYDTETKQIGIEVSMPLFQGGGLNSRRKEAYLRADAADYQYDQAVRDIVQQ
CCHHCCCCCCCCCHHHHEEEEEECCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
TRSAYRTVEADALRIKARQQAIRSTRSALDATQSGYEVGTRNVVDVLNAQRALFAAERDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ANARYDYIINSLTLKSTTGDLQQGDLAAVNNWLAPEQPLDLYNPDLDGKSEKMVPENSSI
CCCHHHHHHHHEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]